GM and KM Immunoglobulin Allotypes in the Galician Population: New Insights Into the Peopling of the Iberian Peninsula
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GM and KM immunoglobulin allotypes in the Galician population: new insights into the peopling of the Iberian Peninsula. Rosario Calderon, Rosa Lodeiro, Tito a Varela, Jose Farina, Beatriz Ambrosio, Evelyne Guitard, Antonio Gonzalez-Martin, Jean-Michel Dugoujon To cite this version: Rosario Calderon, Rosa Lodeiro, Tito a Varela, Jose Farina, Beatriz Ambrosio, et al.. GM and KM immunoglobulin allotypes in the Galician population: new insights into the peopling of the Iberian Peninsula.. BMC Genetics, BioMed Central, 2007, 8 (1), pp.37. hal-00164878 HAL Id: hal-00164878 https://hal.archives-ouvertes.fr/hal-00164878 Submitted on 24 Jul 2007 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. 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GM and KM immunoglobulin allotypes in the Galician population: new insights into the peopling of the Iberian Peninsula BMC Genetics 2007, 8:37 doi:10.1186/1471-2156-8-37 Rosario Calderon ([email protected]) Rosa Lodeiro ([email protected]) Tito A Varela ([email protected]) Jose Farina ([email protected]) Beatriz Ambrosio ([email protected]) Evelyne Guitard ([email protected]) Antonio Gonzalez-Martin ([email protected]) Jean M Dugoujon ([email protected]) ISSN 1471-2156 Article type Research article Submission date 14 March 2007 Acceptance date 27 June 2007 Publication date 27 June 2007 Article URL http://www.biomedcentral.com/1471-2156/8/37 Like all articles in BMC journals, this peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below). Articles in BMC journals are listed in PubMed and archived at PubMed Central. For information about publishing your research in BMC journals or any BioMed Central journal, go to http://www.biomedcentral.com/info/authors/ © 2007 Calderon et al., licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. GMandKMimmunoglobulinallotypesintheGalicianpopulation:newinsightsintothe peoplingoftheIberianPeninsula RosarioCalderón1,RosaLodeiro2,TitoA.Varela2,JoséFariña3,BeatrizAmbrosio1, EvelyneGuitard4,AntonioGonzález-Martín1,JeanM.Dugoujon4 1DepartamentodeZoologíayAntropologíaFísica,FacultaddeBiología,Universidad Complutense,Madrid,Spain. 2DepartamentodeBiologíaAnimal,UnidaddeAntropologíaFísica,FacultaddeBiología, UniversidaddeSantiagodeCompostela,SantiagodeCompostela,Spain 3DepartamentodeEcologíayBiologíaAnimal,ÁreadeAntropologíaFísica,Facultadde Biología,UniversidaddeVigo,Vigo,Spain 4Centred’Anthropologie,FRE2960,CNRS,UniversitéPaulSabatier,Toulouse,France Emailaddresses:RosarioCalderón:[email protected];RosaLodeiro:[email protected];Tito A. Varela: [email protected]; José Fariña: [email protected]; Beatriz Ambrosio: [email protected]; Evelyne Guitard: [email protected]; Antonio González-Martín: [email protected];JeanM.Dugoujon:[email protected] Correspondenceto: RosarioCALDERON Dpto.deZoologíayAntropologíaFísica FacultaddeBiología UniversidadComplutense 28040MADRID(Spain) phone:+34913945111 fax:+34913944947 1 Abstract Background:ThecurrentgeneticstructureofIberianpopulationshaspresumablybeenaffected bythecomplexorographyofitsterritory,thedifferentpeopleandcivilizationsthatsettledthere, itsancientandcomplexhistory,thediverseandpersistentsocioculturalpatternsinitsdifferent regions,andalsobytheeffectsoftheIberianPeninsularepresentingarefugiumareaafterthe last glacial maximum. This paper presents the first data on GM and KM immunoglobulin allotypesintheGalicianpopulationand,thus,providesfurtherinsightsintotheextentofgenetic diversityinpopulationssettledinthegeographicextremesoftheCantabrianregionofnorthern Spain.Furthermore,thegeneticrelationshipsofGalicianswithotherEuropeanpopulationshave beeninvestigated. Results:GalicianpopulationshowsageneticprofileforGMhaplotypesthatisdefinedbythe highpresenceoftheEuropeanMediterraneanGM*3235*haplotype,andtherelativelyhigh incidenceoftheAfricanmarkerGM*1,1723´5*.DatabasedoncomparisonsbetweenGalician andotherSpanishpopulations(mainlyfromthenorthofthepeninsula)revealapoorcorrelation betweengeographicandgeneticdistances(r=0.30,P=0.105),anoticeablebutvariablegenetic distances between Galician and Basque subpopulations, and a rather close genetic affinity between Galicia and Valencia, populations which are geographically separated by a long distanceandhavequitedissimilarculturesandhistories.Interestingly,Galiciaoccupiesacentral positionintheEuropeangeneticmap,despitebeinggeographicallyplacedatoneextremeofthe European continent, while displaying a close genetic proximity to Portugal, a finding that is consistentwiththeirsharedhistoriesovercenturies. Conclusion:ThesefindingssuggestthatthepopulationofGaliciaistheresultofarelatively balancedmixtureofEuropeanpopulationsoroftheancestralpopulationsthatgaverisetothem. ThiswouldsupporttheimportanceofthemigratorymovementsthathavetakenplaceinEurope overthecourseofrecenthumanhistoryandtheireffectsontheEuropeangeneticlandscape. 2 Background Galiciaislocatedatthenorth-westtipoftheIberianPeninsulaandcoversanareaof29424 km2, 5.8% of the total area of Spain. It forms part of the Cantabrian mountain range, which extendsalongthecoastoftheCantabrianSeafromCapeFinisterretothewesternlimitofthe Pyrenees.TheterritoryisborderedtothenorthandwestbytheAtlanticOcean,tothesouthby PortugalandtotheeastbytheregionsofAsturiasandLeon(Figure1). Galicia is currently divided into four provinces: La Coruña, Lugo, Orense and Pontevedra.Itseconomyhastraditionallybeenbasedonagricultureandfishing.Thepopulation of Galicia has a high level of ethnicity as a result of its extreme geographic position and difficultiesofcommunicationwithadjacentregions.Adistinctivecharacteristicoftheregionis thatitpossessesitsownlanguage,Gallego,whichbegantobespokenasalanguagethatcould bedistinguishedfromLatininthe9thCentury.GallegoandPortuguesebecamedifferentiated fromoneanotherasromancelanguagesaroundthemiddleofthe14thcentury. Thepresenceofmegalithicdolmensknownasmámoasthroughouttheregionprovides evidencesupportingtheprehistoricpeoplingofGalicia[1].Theexistenceofsubstantialdeposits ofgold,tin,andcoppermadewayfortheintroductionofmetalworkinthismegalithicculture,a processthatmarkedthebeginningoftheBronzeAgeintheregion,whenpermanentsettlements appeared and commercial relations began via the Atlantic with the British Isles and various Mediterranean populations. Ironwork was introduced to Galicia by the Celts. This people, originatingincentralEurope,appearstohavearrivedintheIberianPeninsulaintwophases,the firstinthe9thcenturyBCandthesecond,whentheypresumablybecameestablishedinGalicia, aroundthe6thcenturyBC[2].TheCeltsdevelopedtheso-called“northerncultureoffortified townsorCastros”thatprevailedinGaliciauntiltheperiodofRomandominationand,mayhave beenthebasisforthecurrentdistributionofthepopulationofGalicia[3].Onlysomeofthepre- Roman languages of the peninsula belonged to the Indo-European group, which extended throughout most of the western and central parts of the peninsula and to which the language spokenatthattimeinGaliciabelonged. 3 RomanizationwasanimportantprocessinthehistoryofEurope,particularlythatofthe Iberian Peninsula. The populations in the north of the peninsula avoided Roman domination until the campaign of Augustus in the 1st century BC and maintained some aspects of their indigenous cultures. Roman domination of Gallaetia gave rise to important communications routes(e.g.viaXXXIVintheItineraryofAntoninus),aswellasanewpoliticalorganizationand restructuring of the population through two important cities, Braga (Portugal) and Lugo (Galicia).ItshouldbenotedthatthemainrouteofcommunicationinthenorthofthePeninsula and in an east–west direction occurred through the flat areas of the central plateau and not through the mountainous costal areas, which were accessed by branches at various points leading off the main route. Thus, this communication system maintained a certain degree of isolationbetweennearbycoastalpopulations. Atthebeginningof5thcentury,whentheRomanEmpirefell,theSwabians,apeopleof German origin, settled for a period of time in Galicia. They lived alongside the native population and at the end of 6th century; the Swabian kingdom was incorporated into the VisigothicHispania.BothSwabiansandVisigothsmaintainedthesocioculturalorganizationof theRomanEmpire. AsoccurredwiththeprocessofRomanization,theimpactoftheMusliminvasionofthe IberianPeninsulaintheyear711waslessinbothGaliciaandothernorthernterritoriesthanin otherregions.Asaconsequence,theregionsofGalicia,northernPortugal,andtheareatothe northoftheRiverDueroactedasimportantrefugesfromtheMusliminvasionduringthe9th and10thcenturies.Thisledtoaprocessofrepopulationthatwasmostrapidinthewesternpart ofGaliciaandPortugal.