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Mass Spectrometric Analysis of Environmental MASS SPECTROMETRIC ANALYSIS OF ENVIRONMENTAL CONTAMINANTS, PROTEIN STRUCTURE AND EXPRESSION IAN E. ATKINSON Bachelor of Science in Zoology Miami University August 1996 Master of Science in Chemistry Cleveland State University May 2001 Submitted in partial fulfillment of requirement for the degree DOCTOR OF PHILOSOPHY IN CLINICAL AND BIOANALYTICAL CHEMISTRY at the CLEVELAND STATE UNIVERSITY DECEMBER, 2008 ©COPYRIGHT BY IAN ERIC ATKINSON 2008 ii This dissertation has been approved for the Department of CHEMISTRY and the College of Graduate Studies by _______________________________________________________ Dissertation Chairperson, Dr. Robert Wei ____________________________ Department & Date _______________________________________________________ Dr. Lily Ng ____________________________ Department & Date _______________________________________________________ Dr. John Masnovi ____________________________ Department & Date _______________________________________________________ Dr. Xiang Zhou ____________________________ Department & Date _______________________________________________________ Dr. Teresa Cutright ____________________________ Department & Date iii Acknowledgements This dissertation is dedicated to my loving parents, Linda & Les, without whose encouragement and support none of this would have been possible. They have taken every opportunity to enrich my life and education, and for that I am beyond thankful. They are truly the most wonderful parents a son could ask for. I would like to deeply thank my advisor, Dr Robert Wei. His direction, guidance and patience have been fundamental in my progress and development as a scientist, a researcher, and an educator. It has been an honor and a privilege working under his tutelage, and I look forward to continuing our work for the good of society and the world. I would like to express how very grateful I am for Dr Lily Ng’s involvement in my life. Her wise counsel and insight has been fundamental in my education. Without her I would have never found my place here at Cleveland State University. I would like to thank Dr Alan Riga and Dr Jerry Mundell, both of whom took a tremendous personal interest in my professional development and have helped prosper a wonderful outlook on science education and research within me. I owe so much of my holistic growth while at Cleveland State to these two gentlemen. I would like to thank Dr Xiang Zhou for so many countless days of training and education on mass spectrometry. I will miss our conversations. I would like to thank my family whose love and concern has motivated me to succeed. My brother David, sister-in-law Kristin, my niece Molly and nephew Colin, all my love, you are the light of my life. My Aunt Sondra, who helped me tremendously with editing this work, my Uncle Joe, my Aunt Wendy, my Uncle Fred, my cousins iv Aaron and Elliot, I cannot express how much your endless encouragement to follow my own path has meant to me. I would like to express my humblest thanks to all of my friends, Adam, Amanda, Ann, Bill, Brian, Carrie, Chad, Curt, Chamari, Deley, Eric, Jason, Jim, Jimmy, Jinhua, John, Kate, Kerri, Lynn, Meg, Mike, Miles, Pam, Pat, Paul, Ram, Rachel, Robert, Brooke & Tony, Isis & Ron, Kelly & Kevin, Meghan & Michael, Melissa & Michael, Nancy & Dick, Nancy & Jeff, Rachel & Ray, Ruth & David, Sheila & Johnny, Wendy & David and many more that I do not have the room to mention here. I started this project in the hopes of taking direction of my dreams to reach a point wherein I could enact change upon the world. It has been my friends support and strength over the years that has held me up, kept my life full and allowed me to see this through. I would also like to express my deep thanks and fullest respect to Mr. Ray Fogg. My mentor, role-model and dear friend, who was very much responsible in my choice of continuing my education and this path I have taken in life. I take the next steps with your inspiration and I follow in your footsteps to ensure tikkun olam. v MASS SPECTROMETRIC ANALYSIS OF ENVIRONMENTAL CONTAMINANTS, PROTEIN STRUCTURE AND EXPRESSION IAN E. ATKINSON ABSTRACT There are a wide array of sciences at our disposal to further our understanding of the roles of toxins in the environment and their effects on living organisms. Of these disciplines, the field of mass spectrometry offers a powerful analytical means to identify compounds in varying and complex sample media. The presence of altered expressions of biomolecules upon exposure to toxins, the effects that these toxins may have on biomolecule structure and the identity of toxins in the sample all can be determined by mass spectrometry. In this study mass spectrometric analysis has been applied to environmental toxicological problems. First, the altered protein expression under various metal stress in Helianthus annuus (dwarf sunflower), a proven hyperaccumulator of toxic metals was analyzed by this research. Second, a model was developed for mass spectrometrically determining the location and structural effects that another class of environmental toxins, poly aromatic hydrocarbons (PAHs) can have on proteins. Third, the presence of PAHs in environmental samples taken from Lake Bolgoda and Lake Beira, Sri Lanka, was analyzed by mass spectrometry to determine the types, relative concentrations and potential sources of these toxins. The work has demonstrated the versatility and effectiveness of the methodology, discovering novel protein expression in H. annuus upon metal exposure, pinpointing site vi mediated adductions reactions on protein structure, and the qualitative and quantitative determination of toxins in environmental samples. vii TABLE OF CONTENTS ABSTRACT……………………..……………………………………………………… vi TABLE OF CONTENTS…………………………………………………………….…viii LIST OF TABLES…………………………………………………………………….…xii LIST OF FIGURES…………...…………………………………………………….…..xiv CHAPTER I LC ESI ION TRAP MS DETERMINATION OF ALTERED PROTEIN EXPRESSION IN HELIANTHUS ANNUUS AFTER EXPOSURE TO HEAVY METALS As, Cd, Cr, Ni AND Pb…………...……………………………..............…….1 1.1. Introduction…………….…………………………………………………………2 1.2. Methodology……………..…………………………………………………….. 13 1.2.1. Plant growth and exposure.………………..………………………………13 1.2.2. Protein extraction and gel separation…..……………...…..………………14 1.2.3. Sample preparation and tryptic digest…..……………………..………….14 1.2.4. LC separation…………………………….…………………..……………16 1.2.5. MS analysis……………………………….…………………..…………...17 1.2.6. MASCOT bioinformatics………………….………………..……………..17 1.3. Results……………….…………………………...……………………………..18 1.3.1. Expression of proteins in Sundance……………………..………………..18 1.3.1.1. Expression of proteins in response to arsenic and other metals in Sundance…………………………………………………………………18 1.3.1.2. Expression of proteins in response to arsenic only in Sundance..24 1.3.1.3. Expression of proteins in response to lead and other metals in viii Sundance………………………………………………………………...25 1.3.2. Expression of proteins in Teddy Bear…………………………….30 1.3.2.1. Expression of proteins in response to arsenic and other metals in Teddy Bear………………………………………………………………30 1.3.2.2. Expression of proteins in response to chromium only in Teddy Bear……………………………………………………………………....30 1.3.2.3. Expression of proteins in response to lead and other metals in Teddy Bear………………………………………………………………31 1.3.2.4. Expression of proteins in response to lead and other metals 2D gel separation in Teddy Bear………………………………………………..34 1.4. Discussion…………………………………………………………………...42 1.5. Conclusions………………………………………………………………….54 1.6. References…………………………………………………………………...58 CHAPTER II STUDY ON ADDUCTION REACTIONS IN CHYMOTRYPSIN’S ACTIVE SITE…………………………………………………………………………...67 2.1. Background and Significance……………………………………………….68 2.2. Methodology………………………………………………………………...77 2.2.1. CHY adduct formation………………….……………………………77 2.2.1.1. CHY-TPCK adduct reaction……...……………………….…….77 2.2.1.2. Proposed CHY-PAH adduct reaction…………………………...77 2.2.2. Whole protein adduct analyses…………………….…………………78 2.2.2.1. LC separation and QQQ analysis……………….……………….78 2.2.2.2. LC separation and Ion Trap Analysis………………...…………79 ix 2.2.3. Hydrazinolysis analysis…………………………….………………...80 2.2.3.1. Hydrazinolysis…………………………………………………..80 2.2.3.2. LC separation and QQQ analysis……………………………….80 2.2.3.3. LC separation and Ion Trap analysis……………………………81 2.2.4. Enzymatic digest analysis……………………………………………83 2.2.5. Functional analysis of protein………………………………………..85 2.3. Results & Discussion 2.3.1. QQQ MS characterization of adduct in chymotrypsin………...…..…85 2.3.2. LC ESI-MS Ion characterization of adduct in chymotrypsin...…..…..90 2.3.3. UV spectroscopic analysis……………..…………………….……….92 2.3.4. Tryptic digest……………………………………..…………………..92 2.4. Conclusions………………………………………………………………….93 2.5. References…………………………………………………………………...95 CHAPTER III MS DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN SEDIMENT AND WATER SAMPLES FROM BOLGODA AND BEIRA LAKES……………………………………………….……..98 3.1. Abstract……………………………………………………………………...99 3.2. Introduction………………………………………………………………...101 3.3. Methodology……………………………………………………………….103 3.3.1. Sample site selection & collection……………...…………………...103 3.3.2. Sample preparation……………………………….…………………105 3.3.3. HPLC Sample analysis…………………………………..………….106 3.3.4. GCMS Sample analysis…………………………………..…………106 x 3.3.5. Evaluation of sediment toxicity…………………………..………...107 3.4. Results & Discussion………………………………………….…..………109 3.4.1. Beira Lake…………………………………………………………..109 3.4.2. Bolgoda Lake…………………………………………….………….113 3.4.3. PAH Ratios……………………………………………….…………116 3.4.4. Microtox Assay…………………………………………….………..119
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