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Original Manufacturer of Antibodies, Proteins, Kits and Reagents Sandwich ELISA ABCB1 ADAM17 ChIP C9orf39 ADAR CCT7 EBI3HMOX2 GSS MAP3K14 MGST1PKIG RPL39L Gene : RASSF1A 3.6 PDCD6 STAR 3.2 RIN2 SYT4 ADAM9CD24 ECH1 2.8 ABCC4CA3 FBXO25 HNMT MIFPLA2G4A RPL9 10 2.4 HADHSC MAP3K6HBB TACC3 ADAMTS1CD34 EDG1 2.0 RNF11STAT6 ABCC6 HOXA9 MKI67PLEKHC1 RPS19 1.6 OD 450 CACNA1C DNM1LHAPLN3 TAF2 ADAMTS17CD48 EEF1A2 1.2 MAPK1PDK2 RNF139STC2 HOXC13 MLL4PLEKHG5 RRM2B 5 0.8 0.4 ABCF2CALCRL DNMT1CUL3 TAF7 ADCY5CDC37 EFNA1PAM Percent Input % 0 MARCKSPDK4 RNF14CNN1 HOXD9 MLLT11PLK2 RPS3MID 0.01 0.1 1 10 100 1000 ABCG1CALR Dnmt3bHBXAP TAGLN3 ADORA3CDC42BPA 0 Recombinant Protein Concentration (ng/ml) HPN PLK4 MARK2PDLIM5 RNF4STK25 EFNB2 MOCS3 PFC ChIP ab: IgG beads MeCP2 ABP1CALR3 DOCK4hCAPD3 RPS3ATAOK1 AEBP1CDH1 MASTLPEX10 RNF8STX5A EHD3HPSE MPHOSPH10PLN Product Name : Human BIRC5 Matched Ab Pair ACACACANT1 DPH1HCAP RPS7TARDBP AHRRCDH18 Product Name : Mecp2 polyclonal antibody Catalog # : H00000332-AP11 MAT2BPFN2 RNPC2STX6 EHD4HRSP12 MRC1PLOD1 Catalog # : PAB0646 Standard curve using recombinant protein CBARA1 DPYDHDAC11 ACHE RRAGCTBL1XR1 AHSA1TCF ChIP results obtained with the Mecp2 (H00000332-P01) as an analyte. MCF2LPGM1 RNU3IP2DAO CDK5R1 EIF2AK3HS6ST1 GPX polyclonal antibody (PAB0646). ACO2CBX2 DSUHDGFRP3 MRPL12PML RRASTBX2 GAA ChIP assays were performed using human MCM7PGM2 ROCK1SUFU AIF1CDK5RAP3 EIF2C3SHC2 osteosarcoma ( U-2 OS ) cells. ACOT9CBX3 DUSP10DDO HSD17B12 MRPL48POLA DND1 HECA MDH1PGM3 ROPN1 RRAS2TBX5 AKAP10CDX2 Flow Cytometry SURF4 ACP1CCNG2 GCA EIF2S1HSF4 MSR1POLI RPS23 HECW2 MED31PGRMC2 TCAP AKR1C4CELSR3 EIF4G 64 RPA3SV2C ACSL1CCNH HSPA1L MT3POLR1C RTKN Immunocytochemistry DUSP6HERC5 MEIS2PHB AKR7A2CEP250 ELAVL1TCF3 RPL10ASWAP70 ACTG2 MTAPPOLR2A RUVBL1HSPB7 CCNK DVL3HHIP MFN1 ALAS1CETN3 ELAVL4HSPE1 PHLDA2 RPL13SYMPK MTMR2POMZP3 S100A11TCF Events CCRN4L DYRK1AHIF1AN ALAS2CETP EMILIN2HSPG2 MFN2PIAS3 RPL14SYNJ2 MTNR1APPARGC1B S100A13 ACTR3CCT2 E2F3HIST1H3D TCF8 ALDH2CGGBP1 EML4 MGAT4APIK3C3 RPL19SYT11 HUWE1 MTSS1PPIG S100A ACVR1CCT5 E2F8HLA-DMA TCOF1 ALOX12BCGREF1 0 100 101 102 103 104 MGAT5PIM3 RPL27ASYT2 ENO3HYAL2 MTUS1PPM1D SAE Green Fluorescence (GRN-HLog) TFEB ALOX15CH25H ENPP3IBRDC2 MUC7PPP1R2 SAMM TFF3 ALPICHD3 ENTPD6IDH2 MUTYHPPP2R2B SCGB Product Name : CCRL2 purified MaxPab mouse TFRC ALPK2CHEK2 ERCC6IFI44L MVPPPP3CA SCN3 polyclonal antibody (B02P) TGFBR2 AMY1ACHMP1B ERRFI1IFT122 MYBPC1 Catalog # : H00009034-B02P Product Name : TUBB3 monoclonal antibody, PPP3CB SCN8ATicam1 ANAPC11CHST11 ERVK2 clone TU-20 FACS analysis of negative control 293 cells (black) IGSF4 MYBPHPRICKLE1 SCN9ATIMM8A ANP32A Catalog # : MAB0907 and CCRL2 expressing 293 cells (green) using CHTF18 ESM1IKBKAP MYCPRKAA2 SDF2TKTL1 CCRL2 purified MaxPab mouse polyclonal AP1M1CIDEB ESPL1IL11RA MYL2PRKCBP1 Immunofluorescence staining of Neuro 2a antibody. mouse neuroblastoma cell line using TUBB3 SDHDTLE6 APEG1CIT ETV4IL1F9 MYL4 monoclonal antibody, clone TU - 20 PRKCD SEC22L1TLK1 API5CLCN2 ( Cat # MAB0907 ; green ) , 3 ug / ml. ETV6IMMT MYLKPRKCDBP SEMA4D Nuclei were stained with DAPI ( blue ) . OneTLR8 APO L3CLGeneCN7 EVLIMP-3 MYST2PRKCI SENP6TMEFF2 in situ Proximity Ligation Assay AQP8CLDN7 EXOC3INPP5A NANS PRKD2 SEP15TMEPAI ARCLEC2D EXOC7INVS NAT5PRKD3 SEPT7ENSA Immunofluorescence ARHGAP4CLIC5 EXT1IPF1 NCAM1PROK2 SERPINE1TNFRSF19 ARHGEF5CLK1 EYA1IRF8 ND1PRPF19 SETTNFSF14 ARHGEF7CLN3 SH2D1A NDE1PRPF3 SETXTNP1 ARID1ACLTB F2RL3ITGA11 OneNDRG4PR UNEAntibodySF1TNPO2 ARL6IP4CMAS FANCGITIH4 NDST1PSCD2 SF3A3TOP1 ARMCX3CNAP1 FATE1ITM1 GDF8 PSIP1 SFRS3TOP2A ART3CNTFR FBXO22ITSN2 NDUFS4PSMA7 SGK3TPBG ARTS-1COL5A1 FBXW11JARID2 NEFHPSMB10 EYA2 TPM4 ARVCFCOL6A2 FBXW8 NEK11PSMB4 SH3BP4TPMT JMJD1C ASAH1CORO1C TPT1 NEK9PSMD10 SH3BP5 FCGR2AK-ALPHA-1 ASB COTL1 FCGR2BKCNA3 NEU1 SH3GL2COVA1 FER1L3 KCNE2 NFAT5PSMD5 SH3GLB1 Product Name : Phospho-RAF1(S259) & RAF1 TRAF4 ASNA1COX4I2 NFIAPSMD9 SH3RF2TRAPPC4 Dual Recognition Antibody Pair KCNE4 ASPSCR1CPB1 KCNH1 NFX1PSTPIP1 SIX2TRIM24 Catalog # : DP0003 ATF5CPNE1 FGGKCNJ1 NFYCPTBP1 SLAMF6TRIM35 Product Name : SMAD3 monoclonal antibody The phosphorylation of RAF1 protein on ATP12ACPSF3 FGL2TPR NGBPTGDR SLBPTRIO ASB9 (M20), clone 4F9 T269 in HeLa cell. Each red dot represents a ATP13A2CPT2 FHKCNK5 NIFUNPTGIR SLC11A2TRIP6 Catalog # : H00004088-M20 phosphorylated protein. ATP2A3CPVL FHL2KCNN3 NLGN4YPTK2B SLC12A6TRPC3 Immunofluorescence of monoclonal antibody to ATP2C1CRB1 FHL5 KCNJ11 NME1PTMA SLC15A1TRPC6 SMAD3 on HeLa cell . ATP5ECREB5 FIS1KIAA0101 NNTPTRF SLC19A2TSPAN7 [antibody concentration 10 ug/ml] CRK FKBP4KIAA1967 NOVA1 PX19 SLC20A1TUBB ATP5G1 FLNAKIF13B NPC1KHDRBS1 SLC22A2TUBB2C ATP6V0A2 CRLF1 ATP6V1ACROP FMNL1 KIF2 NQO1RAB3B FDPS Immunoprecipitation TXNDC4 ATP7BCRTAP FMNL2 KIF2C NRXN1RAB43 FGF8 UBB CRY1 1 2 3 4 NT5C2RAB4A SLC27A4 UBE2D3 ATPIF1 FOLR1 250 KIFC1 BACE1CSF1 FOXC2 KLF13 NT5ERAB5A SLC5A2 Immunohistochemistry 150 UBE2G1 BACH1CSK FPGS KLF2 NUDT1RAB6A SLC9A1 100 SLCO1B1UBE2H BAG2LUM FRG1KLF6 NUMA1RAB7L1 PSMD14 BAIAP2CTBP1 FSHR KNTC1 NUMBRAB8A SLCO2A1 75 UBE2L3 BAIAP2L1 RAPGEF6 FUCA1KNTC2 NUP133CPS1 50 SLKUBE2M BAMBICTNNBL1 FUSIP1KPNA5 OLFM1RALB 37 UBE2T BBOX1CTNND2 FUT2 KRIT1 OLIG2RAMP3 SMNDC1 BBS1CYGB FXYD2KRT4 OPA1 RANGAP1 SMPD2UBR1 OPRL1 25 RAPGEF2 SMURF1UGCG BCHE CYSLTR1 G3BPKRTHB1 BCL2L1 20 DAG1 GABBR1L3MBTL4 OTUB1 RAPGEF3 SNAP23UGT8 BCL9L 15 DAPK2 GABPB2LAMA2 P15RS SNF1LK2ULK2 BCS1LDCPS GAL LASP1 P53AIP1RASGRP4 SOCS5 UMOD BDKRB2DCTN1 GALNT2 PAFAH1B3RBBP4 SORT1UNC5B BGNDCUN1D1 GAPDHSLDLRAP1 Product Name : TERF2 monoclonal antibody, PAICSRBBP6 SOX3UNC5D FZD3 DDX3Y GARTLEPRE1 PANK2 clone 4A794.15 SP100URP2 BICD1DDX6 GAS2 LGALS4 PARD6BRBMS3 SP110 Catalog # : MAB0047 Product Name : CDH17 monoclonal antibody BRCA2DEF6 GDAP1LILRB2 PARP1RBPSUH SPAM1USP33 (M03), clone 3H2 IP / WB Analysis of TERF2 in HL-60 cells BRD2DEFA1 GGPS1LMO2 PARVBRCC1 SPARCUSP4 BRD4 Catalog # : H00001015-M03 ( Lane 1 ) IP with mouse TERF2 monoclonal DEFB4 GKN1LNPEP PBX3 RDH5 SPINLW1VBP1 BRRN1CNN3 antibody GLIPR1LNX2 PCBP1REN SPON2VCL BSCL2DGKH GLRX Immunoperoxidase of monoclonal antibody to ( Lane 2 ) IP with control mouse IgG. LRRK2 PCCBREV3L SPRR3 VDAC1 BTBD2DHFR GNA14LSM16 CDH17 on formalin-fixed paraffin-embedded ( Lane 3 ) IP with goat anti-TRF2. PCDH19RFWD2 SPRY2VPS11 BVESDHX8 GNG2LTBP1 PCDHA5 human colon. [antibody concentration 3 ug/ml] ( Lane 4 ) IP with pre-immune goat Ig. RGL2 SRGAP1VPS41 C10orf7 DHX9 GNG5LY6H PCGF4RGS3 SRPK1VPS4B C1GALT1VRK2 DLL4 GNG7M6PR PCK1RGS4 C20orf31DLX4 GOLGA5MACF PCMT1RHCG SSBP3WASF2 GOT2MAD2L1 PCQAPRHOF SSH3WASL C20orf42DMTF1 C22orf18DNAI2 GPC1MAML PCSK1RHOJ ST3GAL5WDR58 RNAi Knockdown C3DNAJA2 GRID1MAN1B1 PDCD10RICTOR STAM2WDR77 Western Blot Product Name : CACNB1 CHUKFBXL18 LANCL1CPM SDSLUSP31 CHL1FBL KCNQ5 Product Name : AKR1A1 MaxPab mouse polyclonal 1 2 monoclonal antibody (M01), SCYL1UNC13B CHKBFARSLB KCNQ4P2RY12 SCGNUGT2B15 1 2 3 250 clone 1G6 CHI3L1FAM84A JAM2ORC2L SAMD4AUBE2N CFL2EXT2 IRS4 250 antibody (B01) 150 150 100 Catalog # : H00000782- OPRM1 SALL2TXNL1 SSBP1 CFDP1EXOC1 IL2RAOLIG1 S100A6 Catalog # : H00010327- 100 B01 75 M01 TUBG2 CDO1EXO1 BXDC2 OLFM4 RPS6KC1TTC3 CDC42SE2 IL1R2 75 Lane 1: 50 Western blot analysis of ETV1 IL17BNR1H4 TPST1 CD81ERG IL11NPPB RPS14 CACNB1 over-expressed TOB2 CD58ENTPD5 IFT81 NOTCH2NL RPN1TNFSF18 CCL3 50 (Tissue lysate) 37 293 cell line, cotransfected IFNW1NOPE RNUT1DENR CASREMP1 IFI44NLK RNMTTESK1 Western Blot analysis of with CACNB1 Validated TNFAIP8L2 CASP9ELA3A IDH3GNKX6-1 RNF168TMPRSS2 37 AKR1A1 expression in human liver. 25 Chimera RNAi (Lane 2) CARM1EIF4EBP1 IDH3B NKIRAS2 RNF126TMEM49 CANX (H00000782-R01V ) DYNLL2 ID3NETO2 RGL1 TLK2 CAMK4DUOX1 HRG TJP3 Lane 2: 25 or non-transfected control NDUFB11 RFPL1DTYMK HOXD8NAGLU RFP2TIPIN (Transfected lysate) (Lane 1). 20 CABIN1DSG4 HOXC4NAG RETTGFBI C6orf32DPF2 HN1 20 Western Blot Blot probed with CACNB1 15 MYO1B REPS2UBE2G2 C1QTNF1 ENDLINHMGCR analysis of AKR1A1 monoclonal antibody MYL1 RDH11TECTA C1DDNMT2 HLA-DMBMYCN expression in transfected (H00000782-M01). Control RASGEF1CTDG DNAI1 HIST1H2ACMTHFS 15 293T cell line. GAPDH ( 36.1 kDa ) RASEFTCF19 BSGDMPK HERC6 Lane 3: Non-transfected used as specificity and MTF2 RASD1 lysate. loading control. TBRG4 CACNG3 TBK1 TAPBPL www.abnova.com [email protected] Abnova USA Abnova Taiwan Abnova GmbH Tel : +1-877-625-4564 (Toll Free) Tel : +886-2-87511888 Tel : +49-6221-3632226 Fax : +1-909-992-3401 Fax : +886-2-6602-1218 Fax : +49-6221-825878.
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  • Olig1 and Sox10 Interact Synergistically to Drivemyelin Basic

    Olig1 and Sox10 Interact Synergistically to Drivemyelin Basic

    The Journal of Neuroscience, December 26, 2007 • 27(52):14375–14382 • 14375 Cellular/Molecular Olig1 and Sox10 Interact Synergistically to Drive Myelin Basic Protein Transcription in Oligodendrocytes Huiliang Li,1 Yan Lu,2 Hazel K. Smith,1 and William D. Richardson1 1Wolfson Institute for Biomedical Research and Department of Biology, University College London, London WC1E 6BT, United Kingdom, and 2Medical Research Council, Clinical Sciences Centre, Imperial College London, London W12 0NN, United Kingdom The oligodendrocyte lineage genes (Olig1/2), encoding basic helix-loop-helix transcription factors, were first identified in screens for master regulators of oligodendrocyte development. OLIG1 is important for differentiation of oligodendrocyte precursors into myelin- forming oligodendrocytes during development and is thought to play a crucial role in remyelination during multiple sclerosis. However, itisstillunclearhowOLIG1interactswithitstranscriptionalcofactorsandDNAtargets.OLIG1wasreportedlyrestrictedtomammals,but we demonstrate here that zebrafish and other teleosts also possess an OLIG1 homolog. In zebrafish, as in mammals, Olig1 is expressed in the oligodendrocyte lineage. Olig1 associates physically with another myelin-associated transcription factor, Sox10, and the Olig1/Sox10 complex activates mbp (myelin basic protein) transcription via conserved DNA sequence motifs in the mbp promoter region. In contrast, Olig2 does not bind to Sox10 in zebrafish, although both OLIG1 and OLIG2 bind SOX10 in mouse. Key words: Olig1; Olig2; Sox10; Mbp; oligodendrocyte; myelin; zebrafish; mouse; evolution; development Introduction directly regulates Mbp transcription (Stolt et al., 2002), and over- Myelin, the multilayered glial sheath around axons, is one of the expression of SOX10 alone is sufficient to induce myelin gene defining features of jawed vertebrates (gnathostomes). It is expression in embryonic chick spinal cord (Liu et al., 2007).
  • Genome-Wide DNA Methylation Analysis of KRAS Mutant Cell Lines Ben Yi Tew1,5, Joel K

    Genome-Wide DNA Methylation Analysis of KRAS Mutant Cell Lines Ben Yi Tew1,5, Joel K

    www.nature.com/scientificreports OPEN Genome-wide DNA methylation analysis of KRAS mutant cell lines Ben Yi Tew1,5, Joel K. Durand2,5, Kirsten L. Bryant2, Tikvah K. Hayes2, Sen Peng3, Nhan L. Tran4, Gerald C. Gooden1, David N. Buckley1, Channing J. Der2, Albert S. Baldwin2 ✉ & Bodour Salhia1 ✉ Oncogenic RAS mutations are associated with DNA methylation changes that alter gene expression to drive cancer. Recent studies suggest that DNA methylation changes may be stochastic in nature, while other groups propose distinct signaling pathways responsible for aberrant methylation. Better understanding of DNA methylation events associated with oncogenic KRAS expression could enhance therapeutic approaches. Here we analyzed the basal CpG methylation of 11 KRAS-mutant and dependent pancreatic cancer cell lines and observed strikingly similar methylation patterns. KRAS knockdown resulted in unique methylation changes with limited overlap between each cell line. In KRAS-mutant Pa16C pancreatic cancer cells, while KRAS knockdown resulted in over 8,000 diferentially methylated (DM) CpGs, treatment with the ERK1/2-selective inhibitor SCH772984 showed less than 40 DM CpGs, suggesting that ERK is not a broadly active driver of KRAS-associated DNA methylation. KRAS G12V overexpression in an isogenic lung model reveals >50,600 DM CpGs compared to non-transformed controls. In lung and pancreatic cells, gene ontology analyses of DM promoters show an enrichment for genes involved in diferentiation and development. Taken all together, KRAS-mediated DNA methylation are stochastic and independent of canonical downstream efector signaling. These epigenetically altered genes associated with KRAS expression could represent potential therapeutic targets in KRAS-driven cancer. Activating KRAS mutations can be found in nearly 25 percent of all cancers1.
  • SUPPLEMENTAL FIGURE LEGENDS Supplemental Figure S1. RBPJ

    SUPPLEMENTAL FIGURE LEGENDS Supplemental Figure S1. RBPJ

    Xie et al. SUPPLEMENTAL FIGURE LEGENDS Supplemental Figure S1. RBPJ correlates with BTIC marker expression. A-D. The TCGA GBM dataset was downloaded and correlations analyzed by R. RBPJ mRNA levels were highly correlated with (A) Olig2, (B) Sox2, (C) CD133, and (D) Sox4 levels. E. RBPJ is preferentially expressed in proneural glioblastomas. The glioblastoma TCGA dataset was interrogated for RBPJ mRNA expression segregated by transcriptional profile. The proneural tumors were further divided into G-CIMP (glioma CpG-island methylator phenotype) or non-G-CIMP. **, p < 0.01. ****, p < 0.0001. *****, p < 0.00001. Supplemental Figure S2. Targeting RBPJ induces BTIC apoptosis. A. 3691 BTICs were transduced with shCONT, shRBPJ-1, or shRBPJ-2. Lysates were prepared and immunoblotted with the indicated antibodies. shRNA-mediated knockdown of RBPJ was associated with increased cleaved (activated) PARP. B. 3691 BTICs were transduced with shCONT, shRBPJ-1, or shRBPJ-2. Apoptosis measured by Annexin V staining. Data are presented as mean ± SEM (two- way ANOVA; **, p < 0.01; n = 3). Supplemental Figure S3. Targeting RBPJ does not affect non-BTIC proliferation. Non-BTICs (Top, 3691; Bottom, 4121) were transduced with shCONT, shRBPJ-1, or shRBPJ-2. Cell proliferation was measured by CellTiter-Glo. 42 Xie et al. Supplemental Figure S4. RBPJ induces transcriptional profiles in BTICs distinct from Notch activation. A. In parallel experiments, 3691 BTICs were either treated with DAPT (at either 5 μM or 10 μM) vs. vehicle control (DMSO) or transduced with shRBPJ vs. shCONT. RNA-Seq was performed and the results displayed as a heat map with normalization to the relevant control.
  • L-Type Cav 1.2 Calcium Channel-Α-1C Regulates Response to Rituximab in Diffuse Large B-Cell Lymphoma

    L-Type Cav 1.2 Calcium Channel-Α-1C Regulates Response to Rituximab in Diffuse Large B-Cell Lymphoma

    Published OnlineFirst March 1, 2019; DOI: 10.1158/1078-0432.CCR-18-2146 Translational Cancer Mechanisms and Therapy Clinical Cancer Research L-Type Cav 1.2 Calcium Channel-a-1C Regulates Response to Rituximab in Diffuse Large B-Cell Lymphoma Jiu-Yang Zhang1, Pei-Pei Zhang1,Wen-Ping Zhou1, Jia-Yu Yu2, Zhi-Hua Yao1, Jun-Feng Chu1, Shu-Na Yao1, Cheng Wang2, Waseem Lone2, Qing-Xin Xia3, Jie Ma3, Shu-Jun Yang1, Kang-Dong Liu4,5, Zi-Gang Dong4, Yong-Jun Guo3, Lynette M. Smith6, Timothy W. McKeithan7, Wing C. Chan7, Javeed Iqbal2, and Yan-Yan Liu1 Abstract Purpose: One third of patients with diffuse large B-cell an independent prognostic factor for RCHOP resistance after lymphoma (DLBCL) succumb to the disease partly due to adjusting for International Prognostic Index, cell-of-origin rituximab resistance. Rituximab-induced calcium flux is an classification, and MYC/BCL2 double expression. Loss of important inducer of apoptotic cell death, and we investigated CACNA1C expression reduced rituximab-induced apoptosis the potential role of calcium channels in rituximab resistance. and tumor shrinkage. We further demonstrated direct inter- Experimental Design: The distinctive expression of calcium action of CACNA1C with CD20 and its role in CD20 stabi- channel members was compared between patients sensitive lization. Functional modulators of L-type calcium channel and resistant to rituximab, cyclophosphamide, vincristine, showed expected alteration in rituximab-induced apoptosis doxorubicin, prednisone (RCHOP) regimen. The observation and tumor suppression. Furthermore, we demonstrated that was further validated through mechanistic in vitro and in vivo CACNA1C expression was directly regulated by miR-363 studies using cell lines and patient-derived xenograft mouse whose high expression is associated with worse prognosis in models.