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March 2015.Indd NEWSLETTER PP 100000910 ISSN 1442-8725 March 2015 High-dimensional single cell mass cytometry (CyTOF) comes to Australia with the establishment of the Ramaciotti Facility for Human Systems Biology Thomas Ashhurst, Nicholas King, Adrian Smith and Barbara Fazekas de St Groth Understanding immunity through measurements of the single cell Single cell analysis (cytometry) is a critical tool for understanding biological systems composed of heterogeneous cell populations. In such systems, complex interactions between a large number of individual cells defi ne the biological behaviour of the system. Conclusions drawn from bulk measurements that average cellular information – even when they contain many thousands of parameters (e.g. microarray or RNA-seq) – can never provide the degree of mechanistic understanding that is routinely derived from a single cell analysis of the entire population of cells. Important features present in small sub-populations can be obscured or missed completely in a population-based analysis, as can co-variance of multiple characteristics throughout the population. When cancer is involved the situation is even more diffi cult, as heterogeneity exists at all levels from Ramaciotti team (LtoR): Helen McGuire, Thomas Ashhurst, Andrew Mitchell, Barbara Fazekas cancer types to individual patients and even to de St Groth, Nicholas King, Adrian Smith (Photo courtesy Dr Adrian Smith) the point of spatial and/or temporal variation within the one patient. Single cell analysis is by labelling cell surface proteins with Contents therefore a critical technique underpinning fl uorescently-tagged antibodies. Currently, Ramaciotti Facility 1 the analysis of primary cell populations, and high-end cytometers are capable of detecting Editorial 3 provides data that is both complementary up to 18 individual fl uorescent signals Anne Kelso, New CEO of NHMRC 3 to, and necessary for, understanding the simultaneously (with 27-colours reported Obituary – Donald Metcalf 9 biological context of genomic, proteomic on cutting edge fl uorescent platforms), but and functional analyses. the process to achieve this is very complex. Upcoming Conferences 10 Most users can reasonably detect 8-10 President’s Column 11 The challenges of multi parametric single fl uorescent signals simultaneously, with Honorary Secretary’s News 14 cell analysis advanced expertise required for panels of The task of characterising complex 15-18 fl uorescent signals. A combination Visiting Speaker Program 15 heterogeneous cellular populations and of 15 fl uorescently tagged antibodies can Day of Immunology in Victoria 16 their response to disease is inherently identify an enormous range of immune cells 2014 Annual Conference 18 diffi cult, eluding the capacity of our normal in a single sample, enabling pan-leukocyte methodologies for investigating the immune immunophenotyping in various tissues, Councillors’ News 22 system. Using fl ow cytometry, a wide variety Travel Award Conference Reports 25 of cells can be identifi ed and characterised cont.p4 Publications List 30 ASI Inc. Newsletter March 2015 ASI Inc. COUNCIL Non-Voting Councillors: Newsletter Editor President Past President Ms Joanna Roberts Professor Christopher Goodnow Professor Dale Godfrey Ph: 64 6 357 0654 Department Immunology Dept of Microbiology & Immunology Email: [email protected] JCSMR, ANU Peter Doherty Institute PO Box 334, Canberra ACT 2601 University of Melbourne Parkville Vic 3010 Journal Editor Ph: 61 2 6125 2394 Ph: 61 3 8344 6831 Dr Gabrielle Belz Email: [email protected] Email: [email protected] Ph: 61 3 9345 2544 Email: [email protected] Honorary Secretary Honorary Treasurer A/Prof. Stuart Berzins Dr John Stambas Visiting Speakers Co-ordinator CRN Section, School of Health Sciences AAHL, CSIRO Deakin Collaborative Lab. Dr Joanna Kirman Federation University Australia Private Bag 24 Ph: 64 3 479 7712 Mt Helen Vic 3352 East Geelong Vic 3220 Email: [email protected] Ph: 61 3 5320 2039 Ph: 61 3 5227 5740 Email: [email protected] Email: [email protected] Day of Immunology Co-ordinator Dr Claerwen Jones Branch Councillors Ph: 61 3 8344 9595 New South Wales Victoria & Tasmania Email: [email protected] Dr Mainthan Palendira Dr Daniel Gray Ph: 61 2 9565 6211 Ph: 61 3 9345 2497 Meeting Co-ordinator Email: [email protected] Email: [email protected] Dr Susanne Heinzel Ph: 61 3 9345 2609 Queensland South Australia & Northern Territory Email: [email protected] Dr Kristen Radford Dr Cara Fraser Ph: 61 7 3443 7638 Ph: 0422 903 093 Council Member of IUIS Email: [email protected] Email: [email protected] Professor Alan Baxter Ph: 61 7 4781 6265 Western Australia Australian Capital Territory Email: [email protected] Dr Andrew Currie Dr Anselm Enders Ph: 61 8 9360 7426 Ph: 61 2 6125 7605 FIMSA Councillor Email: [email protected] Email: [email protected] Professor Alan Baxter Ph: 61 7 4781 6265 New Zealand Email: [email protected] Dr Roslyn Kemp Ph: 64 3 479 7708 Honorary Archivist: Email: [email protected] Dr Judith Greer Project Manager Ph: 61 7 3346 6018 Miss Sarah Fardy Email: [email protected] Ph: 61 3 5227 5794 / 0413 917 990 ICI2016 Councillor Email: [email protected] Professor Jose Villadangos Administrative Correspondence Ph: 61 3 9035 7684 Ms Judi Anderson Email: [email protected] ASI Inc. Secretariat PO Box 7108 Upper Ferntree Gully Vic 3156 Ph: 61 3 9756 0128 Email: [email protected] The ASI Website The ASI web site (www.immunology.org.au) has been fully remodelled and updated. New services include: ¾ Links providing members with free access to Immunology & Cell Biology, Nature Immunology, Nature Reviews Immunology ¾ Special offers for ASI members ¾ Download and upload forms for ASI awards ¾ Positions vacant page ¾ Online membership renewal ¾ Upcoming conference listings ¾ Women's initiative ¾ Twitter feed as well as many links to sites of immunological interest at home and abroad. If you would like to advertise a job or conference, or if you have an immunology news story, or a favourite immunology-related site that you would like to see linked to the ASI website, please email Sarah Fardy at [email protected] 2 ASI Inc. Newsletter March 2015 EDITORIAL Congratulations to Professor Anne Kelso It is exciting to be the new editor of the ASI quarterly newsletter. Simon Apte, the past – new CEO of the editor, along with Judi Anderson at the ASI Secretariat, have done a brilliant job putting NHMRC this together through the last few years and on behalf of the Society, I thank them whole heartedly for that. They have also both been tremendously helpful as I take on board the ins and outs of this, and thanks also to Sarah Fardy. I’d like to say a huge thanks in advance to Judi, who continues in the role of co-ordinating and preparing this with me. Her work is much appreciated. I asked Simon, as his one last editorial job, to determine the 2014 winner of the best submitted article prize for this newsletter ($200). Margaret Baird is the winner for her contribution on Barbara Heslop. Congratulations Margaret. Australia’s fi rst CyToF features widely A World Health Organisation infl uenza expert in this edition of the newsletter (see lead and celebrated ASI member, Professor Anne article contributed by Thomas Ashhurst Kelso has been appointed the new Chief and colleagues, and Limericks on page Executive Offi cer of the National Health and 18). As a fl ow nerd, I can barely conceal Medical Research Council (NHMRC) and my excitement. This is a great new toy to will take up her position in April. have in our back yard with a really strong to be published here next quarter. We’d love Shared Resource Laboratory (SRL) behind to hear what you get up to. On the announcement, the Minister for it to support users. Health, Sussan Ley said, “Professor Kelso’s I have many ideas about what we can do distinguished career in medical research The Day of Immunology (DOI) is coming with this newsletter – all of them brilliant, and her track record in internationally up fast (Wednesday April 29th) . Be inspired of course (note tongue in cheek) – and I’d be competitive research in immunology and by the article in this edition on the DOI in DELIGHTED to hear from any of you with infl uenza make her an ideal choice to head Victoria and do some great things in your area material or ideas about making this what up the NMHRC.” to promote immunology as well. Then send you want it to be. Send me an email joanna. me a contribution (pictures/words/drawings) [email protected] or give me a call. Anne obtained a Bachelor of Science (Honours) and PhD from the University of Joanna Roberts Melbourne and has developed signifi cant global health experience and networks through her work with the World Health Organisation (WHO). In her previous role as Director of the WHO Collaborating Centre on Infl uenza, Anne worked on surveillance and vaccine policy relating to pandemic viruses ASI Secretariat and provided technical advice to ministers PO Box 7108, of health throughout the Asia-Pacifi c region. Upper Ferntree Gully,Vic. 3156 She was also the Director of the Co-operative Australia Research Centre for Vaccine Technology from 2000 to 2006. Tel: +61 3 9756 0128 Fax: +61 3 9753 6372 In 2004, Anne delivered The Burnet Oration Email: [email protected] at the ASI annual conference entitled ‘Chance and necessity in the immune response’ and she was also ASI President in 1995/96 and ASI Treasurer, 1988-90. 3 ASI Inc. Newsletter March 2015 Ramaciotti Facility (cont) allowing for an analysis of the state of the immune system.
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