Revision: 1 Effective Date: 11/20/2019 Appearance Prepared Appearance Dehydrated Specifications Control Quality 3. 2. 1. Procedure Test 4. 3. 2. 1. Preparation toSDS Refer Precaution specifications. performance to meet required as supplemented and/or adjusted be may Formula 25°C at 0.2 ± 6.7 Final pH: *10 Agar Brom Thiosulfate Sodium Citrate Ammonium Ferric L Dextrose Extract Yeast Gelatin of Digest Enzymatic Typical Formulation lactose rapid detecting for is recommended medium This sulfide. hydrogen abundant produce and lysine deaminate or decarboxylate to ability on their based bacilli enteric differentiating a medium devised Fife and Edwards incorporati and lactose Byeliminating infections. borne in food implicated been it has because H expected to detect medium the who developed Fife, and Edwards of formulation the to according is prepared Agar Lysine Iron Description humans. in other conditions production. sulfide hydrogen and Agar Lysine Iron Use Intended Sheet Specification Technical -

Lysine - blackening at the apex of slant. of apex at the blackening at 18 Examine 18 for at 35°C Incubate conditions. aerobic ensure to tube the Loosely cap medium Inoculate position. a slanted in to solidify medium allow autoclaving, After minutes. 15 for at 121°C autoclave and tubes into test Dispense medium. the dissolve completely to minute one boil for and agitation frequent with Heat water. purified inof one liter the medium of 33 g Suspend 15 g according to gel strength strength gel to according g 15

o cresol Purple cresol

2

S production on Triple Sugar Iron Agar was suppressed. Detection of of Detection suppressed. was Agar Iron Sugar Triple on production S

-

fermenting fermenting

lysine decarboxylase of onbasis the microorganisms of differentiation the is used for arizonae Salmonella –

24 and 40and 24

by stabbing base of tube butt and streaking slant with a needle. a needle. with slant streaking and butt tube of base by stabbing : Prepared medium is red medium : Prepared

S. arizonae S.

: Powder is homogeneous, free flowing, and gray to gray to gray and flowing, free homogeneous, is Powder :

– LysineAgarIron *13.5 g/L *13.5

0.02 g/L 0.02 g/L 0.04 10

Lysine Iron Agar Iron Lysine 48 hours for growth and color changes in tube butt and slant, and for and for slant, and butt tube in changes color and growth for hours 48 0.5 g/L 0.5 1 3 5 .0 .0 .0 .0

.

g/L g/L g/L g/L . S.

arizonae arizonae dish is not intended for use in the diagnosis of disease or disease of diagnosis the in use for intended is not ferments lactose rapidly, and the authors found found authors the and lactose rapidly, ferments purple and trace to slightly hazy. to slightly trace and purple

( NCM0140

)

-

48 hours. hours. 48 S. arizonae S. arizonae - beige.

ng lysine, lysine, ng is important important is

Revision: 1 Effective Date: 11/20/2019 5. 4. 3. 2. 1. References and light. moisture from Protect temperature. place recapped, and opened Once media: culture Dehydrated Storage 3. 2. 1. Procedure the of Limitations container. in its intact medium to applies Expiry color. the original from has changed appearance or if flowing, free discar be should medium dehydrated The on the container. stamped date toexpiration Refer Expiration Results H +, A, acid deamination), (oxidative R, red K, alkaline, KEY: testing. qualitycontrol used thatfor beshould minimum the listed are organisms The Incubation. Cultural Expected Sheet Specification Technical sonnei Shigella typ Salmonella mirabilis Proteus coli Escherichia freundii Citrobacter

20 Chemists. OfficialAnalytical of Association 1 products, 103 p. products, Koenig. E. Donnelly,and C. Andrews, W. S., R. Flowers, W. food, of examination (eds.). Splittstoesser F. D. C.and Vanderzant, MD. Baltimore, & Williams Wilkins, F. J. MacFaddin, Microbiol. Fife. A. M. R., and P. Edwards, incubation. longer of reaction The differentiation. to confirm other media or Agar SugarIron with Triple in conjunction H resu paratyphi Salmonella • • •

2 S th

- A positive hydrogen sulfide reaction is blackened medium at the apex of the slant. atof the apex medium is blackened reaction sulfide hydrogen A positive spp. ( slant. a purple is reaction negative A slant. is a red reaction deaminase lysine A positive (alkaline) slant. purple butt, yellowis (acid) reaction A negative slant. purple butt, (alkaline) is purple reaction decarboxylase lysine A positive ed lting in an alkaline slant and an acid butt. butt. acid an and slant anin alkaline lting producing producing

. AOAC International, Arlington, VA. VA. Arlington, International, AOAC .

and and Microorganism 9: 7 h 478. 478. th ed. American Public Health Association, Washington, D.C. D.C. Association, Washington, Health Public American ed. th

ATCC® imurium Providencia Providencia ATCC® 25922 ATCC®

ATCC® ATCC® Proteus Proteus Morgane Response -

ATCC® 8090 ATCC® 212. 212.

Media for isolation for Media

4th

25931 A, unlike other unlike A, ATCC® 14028 ATCC® In 29906 Store sealed bottle containing the dehydrated medium at 2 medium dehydrated the containing bottle Store sealed spp. do not blacken the medium. It is suggested that Lysine Iron Agar be used be Agar Iron Lysine It is suggested that medium. the blacken not do spp.

R. T. Marshall, (eds.). Standard methods for the examination of dairy of the examination for methods Standard Marshall, (eds.). T. R. lla morganii morganii lla ed. American Public Health Association, Health Public American ed.

spp. produce a red slant over a yellow [acid] butt.) butt.) [acid] yellow a over a redslant produce spp. : Cultural response on Lysine Iron Agar at 35 ± 2°C after 18 after 2°C ± 35 at Agar Lysine Iron on response : Cultural

container in a low humidity low in a container 1961. Lysine 1961.

- cultivatio

may be variable after 23 hours incubation and may require may require and incubation 23 hours after variable be may Salmonella Direct Inoculation Direct Inoculation Direct Inoculation Direct Inoculation Direct Inoculation Direct

Inoculum (CFU) Inoculum 2016

- Approx. Approx. iron agar in the detection of of detection in the agar iron n

- 2015. 2015.

identification . Official methods of analysis of AOAC International, AOAC International, of analysis of methods . Official spp., does not produce lysine decarboxylase decarboxylase lysine produce does not spp., Compendium of methods for the microbiological for the microbiological of methods Compendium 2 S produced, produced, S

environment at the same storage same at the environment - 2004 1. vol bacteria, medial of maintenance

Response Growth Growth Growth Growth Growth

Washington, D.C. D.C. Washington, . Pathogens in milk and milk and milk in milk Pathogens . ---

, H Arizona Arizona

2 S not produced not S

Slant R K K K K

cultures. Appl. cultures. -

30°C. 30°C.

Reactions

– Butt

ded if not not if ded 48 hours 48 A K A K A

Proteus Proteus

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+ + 2 S

Revision: 1 Effective Date: 11/20/2019 7. 6. Sheet Specification Technical

Mosby Company, St. Louis, MO. Louis, St. Company, Mosby J. Martin. and S. M., W. Finegold, manualBAM/default.htm www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical .

1982. Bailey and Scott’s diagnostic , 6th ed. The CV CV The ed. 6th microbiology, diagnostic Scott’s and Bailey 1982.