Aquatic on Line Ver 1 1 of 102
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Aquatic on line ver 1 1 of 102 ! Laboratory Animal Science & Training (Last-Ireland) Training Course Materials Aquatic Module Tutor :Dr T.Murphy Course web page http://www.LAST-Ireland.ie Email [email protected] Aquatic on line ver 1 2 of 102 LAST Ireland online aquatic module for captive fish and frogs Aquatic Module Tutors: Drs T Murphy, Susan Mitchell , Aileen Cronin, Gerhard Schlosser & Peter Nowlan EU Modules Schedule of work study Done Natural History of fish 4 Basic Anatomy and Physiology of fish TM 3.1; 4; 5; Diseases and welfare of fish TM 3.1; 4;5;20;22 Biology of frogs GS 3.14 Biology and Husbandry of farmed fish SM 3.1;4;5 Biology and Husbandry of Zebra Fish Welfare, stress and pain in fish. TM & Humane endpoints 4;5 Euthanasia and humane end-points TM 6.1 Quiz Aquatic on line ver 1 13‐Jan‐20 3 of 102 Introduction to fish biology: A brief natural history Tom Murphy, MVB, MVSc, Ph.D., MRCVS Introduction (i) Natural history of fish Vertebrates : Animals with a backbone or spinal column Five categories of vertebrates: (i) Fish (Salmon, Plaice, Haddock etc) (ii) Amphibians (Frogs etc) (iii) Reptiles (Crocodiles, Snakes) (iv) Birds (Sparrows, Starlings, Hawks etc) (v) Mammals (Horse, Dog, Human etc) Vertebrates Characterised by: (a) Being bilaterally symmetrical (e.g. muscular system consists of paired muscle masses) (b) Central nervous system partially enclosed within a backbone and bony skull Fish: 1st vertebrates: To evolve from the chordates [Notochord evolved to become part of the vertebral column protecting the main nerve] Ancestry: At least 500 million years Fish: Aquatic poikilothermic vertebrates with gills and limbs in the shape of fins and whose bodies are usually covered in scales 1 Aquatic on line ver 1 13‐Jan‐20 4 of 102 Why study fish? Physiology Biochemistry For a fundamental understanding of Developmental Biology general processes Immunology Pathology i) Economic Commercial Fisheries Sport Economic importance ii) Political Fish farming Distribution of fishes: Occupy all aquatic environments (almost everywhere water occurs) Highest diversity : Tropics Ecological Main factor Distribution due to Phylogenetic Factors Continental drift (180-90 millon yrs ago ) Geological Various tectonic plates Fish have successfully conquered almost all naturally occurring aquatic ecosystems: Distribution of fishes: 58% Marine: Ecological division of marine species: i) Epipelagic (surface dwelling (1%)) ii) Deep pelagic (5%) iii) Deep Benthic (6%) iv) Inshore, littoral, continental shelf (46%) 41% Freshwater: Freshwater regions i) Neoartic (N. America) 950 sp. ii) Neotropical (Middle and S. America), 450 sp. Iii) Paleartic (Europe, Asia( north of Himalayans), 500 sp. iv) Africa, 2,900 sp. v) Orient (India, S. China, S.E. Asia), 3,000sp vi) Australia (Australia, New Zealand), 2 sp. 1% Move between freshwater and the marine : 160 sp Anadromous species (Salmon) Northern hemisphere ( High latitudes). 2 Aquatic on line ver 1 13‐Jan‐20 5 of 102 Demonstrated: Physiological In response to specific Anatomical Adaptations environmental Ecological pressures Ocean: Deeper one goes there is: i) Less light ii) Decreasing temperature iii) Less food iv) Increasing pressure Increase up to 20‐400 atmospheres. Fish in Epipelagic, Mesopelagic and Bathypelagic zones: i) Proteins < sensitive to pressure ii) Modified gas bladder (negative buoyancy)/lost in some species iii) Modified musculature + skeleton (no heavy components in their bodies) (little movement) iv) Adaptations of eyes: Large, > sensitivity to light, wavelength 470nm v) Bioluminescence for attracting prey + sexual mates vi) Modified sexual behaviour vii) Metabolic activity: Low Mesopelagic/bathypelagic deep sea fish Anglerfish with two males attached Other features: Large eyes Large expandable mouths Reduced musculature 3 13‐Jan‐20 Aquatic on line ver 1 6 of 102 Epipelagic zone: Numerous fish species: Herring, Sardines, Mackerel, Cod Very productive zone Tuna, Sword fish Fish abundant Fish undergo long migrations Features: i) Counter shaded, silver undersurface ii) Very active: High % red muscle for prolonged swimming iii) Negatively buoyant, have to keep swimming iv) Ram gill ventilation: Mouth open water flows across gills v) Efficient circulatory system, large heart, large blood volume, enhanced O2 to muscles vi) Respiration facilitated by numerous thin gill lamellae vii) Feed on plankton Polar regions: 0 H2O: ‐2.1 C Zone is O2 & nutrient rich a) Blood contains anti freeze glycoproteins b) Kidneys lack glomeruli (stops filtration of glycoproteins) c) Neutral buoyancy: Reduced mineralisation of skeleton( cartilaginous skull and caudal bones) d) Increased lipid deposits throughout the body E) Loss of blood pigments (reduced Hb & myoglobin) f) Increased vascularisation of skin (for O2 exchange Hillstream Loach Fast flowing rivers: [fish hide under rocks etc] a) Dorso ventrally compressed b) Sub‐terminal mouth for feeding on algae on rocks, stones c) Some species: Pelvic fins fused to form a suction disc To be continued: 4 7 of 102 1/21/20 Aquatic on line ver 1 Basic anatomy and physiology of fish T.M. Murphy 1 Basic anatomy Surface anatomy Internal organs 2 Skin: Multipurpose tissue Epidermis Dermis Physical Protection Chemical Metabolically active Epidermis: Stratified squamous epithelium A few cell layers (5-10) thick, non keratinised Covered by a layer of mucus secreted by goblet cells Mucus has antimicrobial properties (first line of defense). Dermis: Stratum spongiosum Also specialised Two layers Dense collagen fibres and scales Chromatophores Stratum compactum Hypodermis: Closest to muscle, collagen fibres and blood vessels 3 1 8 of 102 1/21/20 Aquatic on line ver 1 4 Chromatophores [Melanophores Iridophores ] Specialised cells in the skin Mucous cells [ Antimicrobial activity] Keratocytes [Rapid wound healing] Ion regulated cells Fish mucus: Antimicrobial peptides Still present in mammals including humans Salmon: No. of mucus cells in skin affected by stress and also by gonad development Zebra fish: Ion regulated cells in embryos: Analogous to mammalian kidney tubule cells 5 Types of muscle: Musculature and locomotion i) Skeletal: Striated most of fishes’ mass ii) Smooth: Non-striated, involuntary contractions associated with intestines and blood vessels iii) Cardiac: Non-skeletal, striated (only found in the heart) Two types of skeletal muscle: White: i) Lies on either side of the dorsal column ii) Divided by a horizontal tissue septum ( Epaxial: Upper pair of myomeres) (Hypaxial : Lower pair of myomeres) Red muscle: Wedge shaped under skin along horizontal septum [between the epaxial and hypaxial muscle on each side] 6 2 9 of 102 1/21/20 Aquatic on line ver 1 Skeletal muscle continued Red Muscle White Muscle Aerobic Anaerobic Hard to fatigue Easily fatigued No build up of lactates High build up of lactate Recovery fast Recovery slow, up to 12 hrs Abundant myoglobin Little myoglobin Highly vascularised Little vascularisation Rich oxygen supply Poor oxygen supply Fibres rich in mitochondria Fibres poor in mitochondria 7 8 Blood circulation Heart: Two chambers: (i) Sinus venosus (ii) Atrium (iii) Ventricle (iv) Bulbus arteriosus Various valves between each chamber (to prevent backflow of blood) Bulbus arteriosus: Dampens pulsing (allows a continuous flow of blood) Nerve innervation: Vagus nerve Blood circulation is continuous Only passes through the heart once Deoxygenated blood returns to the heart via afferent blood vessels Then is pumped by the heart through the gill where it’s oxygenated and then to the rest of the body( via Dorsal Aorta) 9 3 10 of 102 1/21/20 Aquatic on line ver 1 Respiration i) Fish pumps H2O over gills ii) Ram ventilation Anatomy: 4 gill arches on each side of the body Large Numerous gill filaments on each arch surface Secondary gill lamellae on the filaments area H2O flows between gill filaments & over the 2ndary lamellae Secondary lamellae : Site of O2 exchange Gas exchange enhanced by: a) Thin epithelial membrane on 2ndary lamellae b) Counter flow of blood [blood flows in opposite direction to water passing over lamellae] 10 Gills and osmoregulation: Process of maintaining an internal balance of salt & H2O in the body Blood hyperosmotic to freshwater Blood hypo-osmotic to seawater Chloride cells: Site of active Na+, Ca++ and Cl- uptake (freshwater) and extrusion (marine) Other cells: Neuro-endocrine cells[paraneurons] [ oxygen sensing cells l][signal transmitted to hypothalamus via glossopharyngeal (ix) and vagus (x) nerves] 11 Gas transport (Oxygen) O2 is transported to the tissues in red blood cells attached to haemoglobin Red blood cells in fish nucleated Haemoglobin (Hb) Molecule : Transports 4 molecules of oxygen Haemoglobin /oxygen transport system: Central to the evolutionary success of teleost fish 12 4 11 of 102 1/21/20 Aquatic on line ver 1 Cell metabolism Basic reaction: CH2O + O2 CH2O3 CO2 + H2O CO2 has to be transported from the tissues to the gills 13 O2 and CO2 transport (Bohr Effect) The Bohr Effect describes how C02 produced in metabolising tissues acidifies the surrounding >pH7.4 <pH 7.4 blood and reduces the affinity of Hb for 02, enhancing the unloading of 02 from Hb to the tissues CO2 C02 transported to the gills as: (i) (i) Bicarbonate (CHO3) in the plasma [70%] (ii) (ii) Attached to proteins in the plasma or attached to the amine group of Hb in the erythrocyte [23%] (iii) (iii) Dissolved in plasma [7%] 14 Bohr Effect versus Root Effect Rete Mirabile: Looping bundle of blood vessels associated with gas bladder that