DOCSLIB.ORG

Isolation and Characterization of a Multiple Herbicide Resistant

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Isolation and Characterization of a Multiple Herbicide Resistant Indian Journal of Biotechnology Vol 11, January 2012, pp 77-85 Isolation and characterization of a multiple herbicide resistant strain [Av(MHR)Ar,Al,B,D] of diazotrophic cyanobacterium Anabaena variabilis Surendra Singh, Pallavi Datta* and Archna Tirkey Algal Biotechnology Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur 482 001, India Received 1 August 2010; revised 18 December 2010; accepted 20 February 2011 The present study was aimed to isolate a multiple herbicide resistant strain [(MHR)Ar,Al,B,D] of Anabaena variabilis exhibiting resistance against four common rice-field herbicides, viz., Arozin, Alachlor, Butachlor and 2,4-D. The multiple herbicide resistant (MHR) strain was isolated by spontaneous mutational techniques. Of the four spontaneously occurring mutants, Arozin resistant mutant (Ar-R) exhibited 92-98% survival in lethal concentrations of Arozin, Alachlor, Butachlor and 2,4-D, and was designated as MHR strain, Av(MHR)Ar,Al,B,D. The MHR strain exhibited faster growth rate, substantial increase in macromolecular contents, photosynthesis, nitrogenase and glutamine synthetase (GS) activity not only in graded concentration of herbicides but below (0-25 mg L-1) and above the lethal dosages (25-80 mg L-1), and even in the presence of combined lethal dosages of all four herbicides as compared to wild type as well as Alachlor, Butachlor and 2,4-D resistant strains. The cross resistance relationship in Arozin resistant (Ar-R) mutant strain of A. variabilis with lethal dosages of Alachlor, Butachlor and 2,4-D did indicate that it was a single mutational event and resistance to Arozin was associated with the acquisition of resistance to Alachlor, Butachlor and 2,4-D. Evidently such improved Av(MHR)Ar,Al,B,D strain of diazotrophic cyanobacterium A. variabilis under herbicide(s) stressed agro-ecosystem, particularly rice-fields, would likely to serve as efficient biofertilizer. Keywords: Biofertilizer, cyanobacteria, herbicides, mutant Introduction Some reports are available on genetic improvement Diazotrophic cyanobacteria (blue green algae) have in certain cyanobacteria through mutagenesis been implicated in sustaining the cultivation of conferring herbicide (Norflurazon, Fluridone, wetland rice for centuries because of their inherent Dinoseb, Amitrole) resistance to Synechococcus sp. ability to add fixed nitrogen to such habitats under PCC 7942 and Synechocystis sp. PCC 68039-11. aerobic photosynthetic conditions1-3. This has led to Gloeocapsa sp., a natural isolate, showing resistance the development of biofertilizer technology involving against two rice field herbicides, Machete (Butachlor) inoculation of the paddy fields with suitable and Basalin12 and Nostoc muscorum exhibiting diazotrophic cyanobacterial strains4-7. In recent years resistance against Amitrole and Carbendazine13 have the practice of using diazotrophic cyanobacteria as an been isolated. Evidently most of the work has been efficient source of nitrogenous biofertilizer for rice concentrated on isolation of cyanobacterial mutants crops has been advocated and adapted in many through mutagenesis exhibiting resistance either developing countries8. However, the productivity of against one or two herbicides. Hence, there is a need the wetland rice agriculture is heavily dependent on to develop more and more herbicide resistant the periodic and extensive use of highly toxic diazotrophic cyanobacterial strains native to rice field herbicides for selective elimination of weeds of rice ecosystem by mutational techniques conferring crops. The extreme sensitivity of cyanobacteria to resistance to a number of rice-field herbicides. The rice-field herbicides is limiting the wide utilization of concept of developing multiple herbicide resistant cyanobacterial biofertilizer technology. A successful strains of diazotrophic cyanobacteria is important biotechnology involving the use of N2-fixing since combination of herbicides are applied in modern cyanobacteria as biofertilizer in modern rice rice agriculture and the residual toxic effects of these agriculture would require them to have the additive agrochemicals persists in the environment14,15. property of resistance to various rice field herbicides. Therefore, efforts have been made to develop a __________ multiple herbicide resistant strain (MHR) of a natural *Author for correspondence: Tel: +91-9300126788; Fax: +91-761-603752. diazotrophic cyanobacterial isolate Anabaena E-mail: [email protected] variabilis by isolating and examining cross resistant 78 INDIAN J BIOTECHNOL, JANUARY 2012 relationship among the spontaneous mutant clones conditions as mentioned earlier. After 3-4 wk, exhibiting resistance against four common rice-field cross-resistant colonies appearing on the surface of herbicides, viz., Arozin, Alachlor, Butachlor and agar plates were picked up and transferred to sterile 2,4-D. Arozin resistant mutant of A. variabilis exhibited N2-medium for the growth. Among the four herbicide cross resistance against lethal dosages of Alachlor, resistant mutants tested, Arozin resistant mutant Butachlor and 2,4-D. This multiple herbicide resistant (Ar-R) clones survived in the lethal concentrations of mutant exhibited enhanced growth, survivability, Alachlor, Butachlor and 2,4-D. This mutant was N2-fixation, photosynthesis and other vital metabolic considered to be cross resistant and was designated as activities as compared to wild type strain. MHR strain [Av(MHR)Ar,Al,B,D]. Butachlor (B-R), Alachlor (Al-R) and 2,4-D resistant (D-R) strains Materials and Methods could not survive in lethal dosages of other Organism and Growhth Conditions herbicides. For spontaneous reversion studies, The axenic clonal culture of N2-fixing exponentially growing culture of MHR strain was cyanobacterium A. variabilis, a rice field isolate16, harvested by repeated centrifugation (5000× g; 17 was routinely grown in BG11 medium devoid of any 5 min) and washed with sterile double distilled water combined nitrogen source (N2-medium). Cultures and resuspended in fresh N2-medium. Inoculum 5 were incubated for the growth in an air-conditioned (3 × 10 CFU) was plated on solid N2-medium with or culture room maintained at 25±1°C fitted with cool without herbicide treatment and incubated for 15 d day fluorescent light. Photon flux density of light on under photo autotrophic growth conditions for scoring the surface of the vessel was 45 µE m-2 s-1 for 18 h d-1. revertants. The same experiment was repeated thrice. No change in the colonial growth in both the Mutant Isolation treatment was recorded. A. variabilis did not survive beyond a concentration -1 Characterization of MHR Strain in Graded Concentrations of of 25 mg L of Arozin, Alachlor, Butachlor and Herbicides 2,4-D. Accordingly, its diazotrophically grown The MHR strain was characterized in terms of 7 cultures (5.0 × 10 CFU) were seeded per diazotrophic following parameters: nutrient plate containing 50 mg L-1 Arozin, 75 mg L-1 -1 -1 Determination of Growth Kinetics Butachlor, 75 mg L 2,4-D and 100 mg L Alachlor Growth kinetics of MHR strain along with wild to select out spontaneously occurring herbicide type A. variabilis was determined in diazotrophic resistant mutant clones. Colonies of the mutant growth conditions by estimating changes in appearing on the respective nutrient plates containing chlorophyll a and total protein content at regular herbicide were tested 3-4 times for their stability by interval of 24 h19,20 up to 12 d of growth. Generation streaking them on fresh nutrient plates containing time and specific growth rate was calculated using the same concentration of herbicides. Stable Arozin following formula: (Ar-R), Butachlor (B-R), 2,4-D (D-R) and Alachlor resistant (Al-R) mutant clones thus obtained were Kt = log10 (Nt/N0) grown and maintained in N2-medium along with their Where, K = Growth rate constant, t = Growth period, wild type strains under photoautotrophic growth Nt = Absorbance at time t, No = Absorbance at time 0. conditions as described above. 24 Generation time = Examination of Cross Resistant Relationship among Various Specific growth rate cons tan t Herbicide Resistant Mutant Strains of A. variabilis and Isolation of Multiple Herbicide Resistant (MHR) Mutant Determination of Macromolecular Contents Strain [Av (MHR)Ar,Al,B,D] Macromolecular contents, i.e., chlorophyll a, To establish cross resistant relationship between protein, carotenoids, phycocyanin, phycoerythrin, Arozin, Alachlor, Butachlor and 2,4-D resistant carbohydrate, DNA and RNA were determined at strains of A. variabilis, exponentially grown (6-d-old) regular intervals of 48 h of diazotrophic growth19-24. cultures of each resistant strain was plated on solid agar (2.0% w/v) based N2-medium containing lethal Determination of Heterocyst Frequency concentrations18 of all four herbicides individually. Heterocyst frequency of exponentially grown The plates were incubated in photoautotrophic growth diazotrophic culture of both MHR and wild type SINGH et al: ISOLATION AND CHARACTERIZATION OF A MULTIPLE HERBICIDE RESISTANT STRAIN 79 strains was determined microscopically and expressed Results in percentage as total number of heterocysts occurring The N2-fixing culture of wild type A. variabilis per 100 vegetative cells. exhibited a decrease in survival with increase in the concentrations of herbicides and reached almost a -1 Determination of Photosynthetic O2 Evolution and Dark O2 zero percent survival in 25 mg L of Arozin, Uptake Butachlor, Alachlor and
Load more

Recommended publications
  • 2,4-Dichlorophenoxyacetic Acid
    2,4-Dichlorophenoxyacetic acid 2,4-Dichlorophenoxyacetic acid IUPAC (2,4-dichlorophenoxy)acetic acid name 2,4-D Other hedonal names trinoxol Identifiers CAS [94-75-7] number SMILES OC(COC1=CC=C(Cl)C=C1Cl)=O ChemSpider 1441 ID Properties Molecular C H Cl O formula 8 6 2 3 Molar mass 221.04 g mol−1 Appearance white to yellow powder Melting point 140.5 °C (413.5 K) Boiling 160 °C (0.4 mm Hg) point Solubility in 900 mg/L (25 °C) water Related compounds Related 2,4,5-T, Dichlorprop compounds Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) 2,4-Dichlorophenoxyacetic acid (2,4-D) is a common systemic herbicide used in the control of broadleaf weeds. It is the most widely used herbicide in the world, and the third most commonly used in North America.[1] 2,4-D is also an important synthetic auxin, often used in laboratories for plant research and as a supplement in plant cell culture media such as MS medium. History 2,4-D was developed during World War II by a British team at Rothamsted Experimental Station, under the leadership of Judah Hirsch Quastel, aiming to increase crop yields for a nation at war.[citation needed] When it was commercially released in 1946, it became the first successful selective herbicide and allowed for greatly enhanced weed control in wheat, maize (corn), rice, and similar cereal grass crop, because it only kills dicots, leaving behind monocots. Mechanism of herbicide action 2,4-D is a synthetic auxin, which is a class of plant growth regulators.
    [Show full text]
  • Response of Multiple Herbicide Resistant Strain of Diazotrophic Cyanobacterium, Anabaena Variabilis , Exposed to Atrazine and DCMU
    Indian Journal of Experimental Biology Vol. 49, April 2011, pp.298-303 Response of multiple herbicide resistant strain of diazotrophic cyanobacterium, Anabaena variabilis , exposed to atrazine and DCMU Surendra Singh, Pallavi Datta * & Archna Tirkey Algal Biotechnology Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur 482 001, India Received 1 June 2010; revised 24 January 2011 Effect of two photosynthetic inhibitor herbicides, atrazine (both purified and formulated) and [3-(3,4-dichlorophenyl)- 1,1-dimethyl urea] (DCMU), on the growth, macromolecular contents, heterocyst frequency, photosynthetic O 2 evolution and dark O 2 uptake of wild type and multiple herbicide resistant (MHR) strain of diazotrophic cyanobacterium A. variabilis was studied. Cyanobacterial strains showed gradual inhibition in growth with increasing dosage of herbicides. Both wild type and MHR strain tolerated < 6.0 mg L -1 of atrazine (purified), < 2.0 mg L -1 of atrazine (formulated) and < 0.4 mg L -1 of DCMU indicating similar level of herbicide tolerance. Atrazine (pure) (8.0 mg L -1) and 4.0 mg L -1 of atrazine (formulated) were growth inhibitory concentrations (lethal) for both wild type and MHR strain indicating formulated atrazine was more toxic than the purified form. Comparatively lower concentrations of DCMU were found to be lethal for wild type and MHR strain, respectively. Thus, between the two herbicides tested DCMU was more growth toxic than atrazine. At sublethal dosages of herbicides, photosynthetic O 2 evolution showed highest inhibition followed by chlorophyll a, phycobhiliproteins and heterocyst differentiation as compared to carotenoid, protein and respiratory O 2 uptake. Keyword s: Atrazine, Cyanobacteria, DCMU, Herbicides, Mutants, Photosynthesis In modern agriculture weed control by herbicides is a tolerate or resist toxic actions of various rice field common practice to increase in crop productivity 1.
    [Show full text]
  • Multi-Residue Method I for Agricultural Chemicals by LC-MS (Agricultural Products)
    Multi-residue Method I for Agricultural Chemicals by LC-MS (Agricultural Products) 1. Analytes See Table 2 or 3. 2. Instruments Liquid chromatograph-mass spectrometer (LC-MS) Liquid chromatograph-tandem mass spectrometer (LC-MS/MS) 3. Reagents Use the reagents listed in Section 3 of the General Rules except for the following. 0.5 mol/L Phosphate buffer (pH 7.0): Weigh 52.7 g of dipotassium hydrogenphosphate (K2HPO4) and 30.2 g of potassium dihydrogenphosphate (KH2PO4), dissolve in about 500 mL of water, adjust the pH to 7.0 with 1 mol/L sodium hydroxide or 1 mol/L hydrochloric acid, and add water to make a 1 L solution. Reference standards of agricultural chemicals: Reference standards of known purities for each agricultural chemical. 4. Procedure 1) Extraction i) Grains, beans, nuts and seeds Add 20 mL of water to 10.0 g of sample and let stand for 15 minutes. Add 50 mL of acetonitrile, homogenize, and filter with suction. Add 20 mL of acetonitrile to the residue on the filter paper, homogenize, and filter with suction. Combine the resulting filtrates, and add acetonitrile to make exactly 100 mL. Take a 20 mL aliquot of the extract, add 10 g of sodium chloride and 20 mL of 0.5 mol/L phosphate buffer (pH 7.0), and shake for 10 minutes. Let stand, and discard the separated aqueous layer. Add 10 mL of acetonitrile to an octadecylsilanized silica gel cartridge (1,000 mg) and discard the effluent. Transfer the acetonitrile layer to the cartridge, elute with 2 mL of acetonitrile, collect the total eluates, dehydrate with anhydrous sodium sulfate, and filter out the anhydrous sodium sulfate.
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 7,943,644 B2 Uhr Et Al
    USOO794364.4B2 (12) United States Patent (10) Patent No.: US 7,943,644 B2 Uhr et al. (45) Date of Patent: May 17, 2011 (54) STABILIZATION OF IODINE-CONTAINING (56) References Cited BOCDES BY MEANS OF SPECIAL AZOLE COMPOUNDS U.S. PATENT DOCUMENTS 2,739,922 A * 3/1956 Shelanski ..................... 524,548 (75) Inventors: Hermann Uhr, Leverkusen (DE); 4,276,211 A 6/1981 Singer et al. ... 260/29.6 MN Johannes Kaulen, Odenthal (DE); 4.297.258 A 10/1981 Long, Jr. .............. 260f29.6 MN Thomas Jaetsch, Köln (DE); Peter 4,552,885. A 1 1/1985 Gabriele et al. .............. 514/316 Spetmann, Leverkusen (DE) 5,051,256 A * 9/1991 Barnes ........... ... 424/402 6,143,204. A 1 1/2000 Lutz et al. ...... ... 252/384 rsr rr 6.353,021 B1 3/2002 Gaglani et al. ... 514,478 (73) Assignee: NNESS putschland GmbH, 6,472,424 B1 10/2002 Gaglani et al. ... 514,478 everkusen (DE) 6,946,427 B2* 9/2005 Lutz et al. ...... ... 504,140 c - 2006/00 13833 A1 1/2006 Bartko ........... ... 424/400 (*) Notice: Subject to any disclaimer, the term of this 2007/0128246 A1* 6/2007 Hossainy et al. ............. 424/423 patent is extended or adjusted under 35 U.S.C. 154(b) by 18 days. FOREIGN PATENT DOCUMENTS WO 98.22543 5, 1998 (21) Appl. No.: 12/281,163 WO 99.291.76 6, 1999 WO OOf 16628 3, 2000 (22) PCT Filed: Feb. 21, 2007 WO 2007 O28527 3, 2007 (86). PCT No.: PCT/EP2007/001480 OTHER PUBLICATIONS S371 (c)(1), Nomiya, Kenji, et al.
    [Show full text]
  • Increasing the Efficacy and Safety of Butachlor and Its 2,4-D Mixture in Direct Wet-Seeded Rice (Oryza Sat/Val.)
    Trans. Nat. Acad. Science & TechnoL 1984.6:43-50 INCREASING THE EFFICACY AND SAFETY OF BUTACHLOR AND ITS 2,4-D MIXTURE IN DIRECT WET-SEEDED RICE (ORYZA SAT/VAL.) Ponciano M. Halos* and R.W. Schumacher** "'Monsanto Philippines, Inc. "'"'Monsanto Singapore Company (Pte.) Ltd. ABSTRACT On pre-germinated wet seeded rice, the 24 days before seeding (DBS) treatments for butachlor (1 kg. aj./ha.) and 6-8 DBS treatment for the butachlor + 2,4-D combination (0.15 + 0.5 kg. a.i./ha.) provided improved crop safely, bet­ ter control of grasses, sedges and broadleaves and higher yields compared with the 6-9 days after seeding herbicide treatments. Screen (R) solution, when properly coated on seeds before germination prior to sowing at 1.3 ml./kg. and at higher concentrations effectively served as rice seed safener in field plots treated with the increased concentrations of 2 kg. a.i./ha. butachlor and 1 + 0.67 kg. a.i./ha. butachlor + 2,4-D even when these herbicides were applied at 2 days before seeding. futroduction Direct seeding of pre-germinated rice under puddled condition offers several advantages over transplanting. These include dramatic reduction in production costs and manpower requirements and increased frequency of planting. The latter is especially relevant in the "Kabsaka Program" on 235,000 hectares in Iloilo wherein two rice plantings are targeted per wet season to realize increased income for the farmers. This necessitates direct seeding as the practical approach due to the time constraints within the seven months wet period. Also, with the opening up of the Magat River Irrigation Project in Northem Luzon, there will be very limited time to plant the additional 75-80 thousand hectares.
    [Show full text]
  • Committee for Risk Assessment RAC Annex 3 Records of the Targeted
    Committee for Risk Assessment RAC Annex 3 Records of the targeted public consultation on the reproductive toxicity of Acetochlor (ISO); 2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6- methylphenyl)acetamide EC number: 251-899-3 CAS number: 34256-82-1 CLH-O-0000001412-86-26/F 1 The proposal for the harmonised classification and labelling (CLH) of acetochlor was submitted by Spain in September 2013; it was subject to public consultation from 5 December 2013 until 20 January 2014. The comments received by that date are compiled in Annex 2 to this opinion. After the closure of the public consultation, the Committee for Risk Assessment (RAC) noted that the data from the repeated dose toxicity studies as well as from the reproductive toxicity studies indicated that classification for reproductive toxicity should be considered for acetochlor . As the Dossier Submitter initially did not propose classification for reproductive toxicity, and in order to strengthen the information base, ECHA launched an additional public consultation focussing on the potential reproductive toxicity of acetochlor. The consultation started on 18 September 2014 and finished on 6 October 2014. The comments received are compiled in this annex. 2 COMMENTS AND RESPONSE TO COMMENTS RECEIVED DURING THE TARGETED CONSULTATION (18 SEPTEMBER – 6 OCTOBER 2014) Substance name: Acetochlor (ISO); 2-chloro-N-(ethoxymethyl)-N-(2-ethyl-6-methylphenyl)acetamide CAS number: 34256-82-1 EC number: 251-899-3 Date Country / Comment RAC response to comment Person / Organisation / MSCA 06/10/2014 French CA France is of the opinion that a classification of acetochlor for fertility Thank you for your comments.
    [Show full text]
  • Classification of Herbicides
    Title of the course : Weed Management Credit: 3(2+1) Class : 3rd Year IInd Semester Title of the topic : Principles of weed management College : Krishi vigyan Kendra,College of Agriculture, Rewa, JNKVV, Jabalpur Name of Teacher : Dr. (Mrs.) Smita Singh Classification of Herbicides Herbicides: Chemical method of weed control is very effective in certain cases and have great scope provided the herbicides are cheap, efficient and easily available. The chemicals used for killing the weeds or inhibiting growth of weeds are called herbicides (Weedicides). Classification of Herbicides: Herbicides are classified in different ways: A) First Group Chemical Herbicides: I) Classification of herbicides according to chemical composition. II) Classification of herbicides according to their use. III) Classification of herbicides based on time of application. IV) Classification of herbicides according to Formulation. V) Classification of herbicides according to residual effect. B) Second Group – Bio herbicides C) Third Group herbicidal mixtures. Classification of herbicide I) Classification of Herbicide Based on Chemical Nature or Composition Compounds having chemical affinities are grouped together. This is useful in liting and characterising herbicides. i) Inorganic Herbicides:Contain no carbon actions in their molecules. These were the first chemicals used for weed control before the introduction of the organic compounds, example are: a) Acids:Arsenic acid, arsenious acid, arsenic trioxide sulphuric acid. b) Salts:Borax, copper sulphate, ammonium sulphate, Na chlorate , Na arsenite , copper nitrate. ii) Organic Herbicides:Oils and non oils contain carbon and hydrogen in their molecules. a) Oils: Diesel oil, standard solvent, xylene-type, aromatic oils, polycyclic , aromatic oils etc. b) Aliphatics:Dalapon, TCA, Acrolein, Glyphosphate methyl bromide.
    [Show full text]
  • Recommended Classification of Pesticides by Hazard and Guidelines to Classification 2019 Theinternational Programme on Chemical Safety (IPCS) Was Established in 1980
    The WHO Recommended Classi cation of Pesticides by Hazard and Guidelines to Classi cation 2019 cation Hazard of Pesticides by and Guidelines to Classi The WHO Recommended Classi The WHO Recommended Classi cation of Pesticides by Hazard and Guidelines to Classi cation 2019 The WHO Recommended Classification of Pesticides by Hazard and Guidelines to Classification 2019 TheInternational Programme on Chemical Safety (IPCS) was established in 1980. The overall objectives of the IPCS are to establish the scientific basis for assessment of the risk to human health and the environment from exposure to chemicals, through international peer review processes, as a prerequisite for the promotion of chemical safety, and to provide technical assistance in strengthening national capacities for the sound management of chemicals. This publication was developed in the IOMC context. The contents do not necessarily reflect the views or stated policies of individual IOMC Participating Organizations. The Inter-Organization Programme for the Sound Management of Chemicals (IOMC) was established in 1995 following recommendations made by the 1992 UN Conference on Environment and Development to strengthen cooperation and increase international coordination in the field of chemical safety. The Participating Organizations are: FAO, ILO, UNDP, UNEP, UNIDO, UNITAR, WHO, World Bank and OECD. The purpose of the IOMC is to promote coordination of the policies and activities pursued by the Participating Organizations, jointly or separately, to achieve the sound management of chemicals in relation to human health and the environment. WHO recommended classification of pesticides by hazard and guidelines to classification, 2019 edition ISBN 978-92-4-000566-2 (electronic version) ISBN 978-92-4-000567-9 (print version) ISSN 1684-1042 © World Health Organization 2020 Some rights reserved.
    [Show full text]
  • Download Article (PDF)
    Isolation and Characterization of a Chlamydomonas reinhardtii Mutant Resistant to Photobleaching Herbicides Hiromichi Oshioa, Hideyuki Shibata*, Nobuaki Mitoa, Masako Yamamoto2, Elizabeth H. Harris*5, Nicholas W. Gillhamb, John E. Boyntonb, and Ryo Satoa b a Agricultural Science Research Laboratory, Takarazuka Research Center, Sumitomo Chemical Co. Ltd., Takatsukasa, Takarazuka, Hyogo 665, Japan b Departments of Botany and Zoology, Duke University, Durham, NC 27708-0338, U.S.A. Z. Naturforsch. 48c, 339-344 (1993); received November 5, 1992 N-Phenylimide Herbicide, Photobleaching Herbicide, Protoporphyrin IX, Protoporphyrinogen Oxidase, Chlamydomonas reinhardtii A group of highly active N-phenylimide photobleaching herbicides have been synthesized. These N-phenylimide herbicides as well as diphenyl ether herbicides induce protoporphyrin IX accumulation and inhibit protoporphyrinogen oxidase activity at extremely low concentrations in higher plants. The binding of a , 4C-labeled N-phenylimide herbicide S-23121 [N-[4-chloro- 2-fluoro-5-[(l-methyl-2-propynyl)oxy]phenyl]-3,4,5,6-tetrahydrophthalimide] to the solubil­ ized plastid fractions of greening corn seedlings is competed by the diphenyl ether herbicide acifluorfen-ethyl, but not by diuron, an inhibitor of photosynthetic electron transport. These results indicate a similar mode of action for both N-phenylimide and diphenyl ether herbi­ cides. In order to investigate the mechanism of photobleaching herbicides at the molecular level, a strain o f Chlamydomonas reinhardtii RS-3 resistant to N-phenylimide S-23142 [N-(4-chloro- 2-fluoro-5-propargyloxyphenyl)-3,4,5,6-tetrahydrophthalimide] was isolated by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. The 90% inhibition concentration of N-phenyl­ imide S-23142 for growth of RS-3 was 100 times higher than that for wild type.
    [Show full text]
  • Conditions of Paraquat Use in India Contacts
    CONDITIONSDILEEP KUMAR OF PARAQUAT A D USE IN INDIA AprilApril 2015 2015// 1 CONDITIONS OF PARAQUAT USE IN INDIA CONTACTS Pesticide Action Network (PAN) India IUF/UITA Vilangottil House 8 Rampe du pont rouge, House No. 104 1213 Petit Lancy, Switzerland Prayaga, Warriam Road Thalore P.O. Thrissur, Phone +41 22 793 2233 Kerala, India Fax +41 22 793 2238 PIN-680306 [email protected] www.iuf.org [email protected] www.pan-india.org Berne Declaration Dienerstrasse 12 PAN Asia and the Pacific Postfach P.O. Box 1170 8026 Zurich, Switzerland 10850 Penang, Malaysia Phone +41 44 277 70 00 Phone 604-6570271 or 604-6560381 Fax +41 44 277 70 01 Fax 604-6583960 [email protected] [email protected] www.evb.ch www.panap.net Frontpage A farmer with a hand operated sprayer (West Bengal). Often paraquat and other pesticides are applied with this kind of sprayer. IMPRINT Author Dileep Kumar A. D. | Edited by Sreedevi Lakshmikutty | Photos Dileep Kumar A. D.: Frontpage, page 17, 25; Kondala Reddy: page 5, 30; IUF: page 7, 9, 29 | Design Karin Hutter CONDITIONS OF PARAQUAT USE IN INDIA April 2015 // 3 FOREWORD BY THE EDITORS For many years, IUF, Pesticide Action Network and the trinsic hazards and a realistic assessment of the common Berne Declaration have been asking governments for conditions of use. a global ban on paraquat, and also calling on industry to The editors would like to thank the main author, Dileep stop the production and sale of this highly hazardous Kumar A. D., the whole team of PAN India, and all the oth- pesticide.
    [Show full text]
  • Public Health Assessment: Highway 36 Corridor Exposure Investigation
    Final Public Health Assessment Highway 36 Corridor Exposure Investigation October 2014 Prepared by the Environmental Health Assessment Program Oregon Health Authority; Public Health Division i Funded under a cooperative agreement with the Agency for Toxic Substances and Disease Registry. (Grant no. 280603) ii Oregon Health Authority - Highway 36 Public Health Assessment October 2014 Foreword The Oregon Health Authority (OHA), in cooperation with state and federal partners, prepared this Public Health Assessment (PHA). The Agency for Toxic Substances and Disease Registry (ATSDR) and its Oregon cooperative agreement partner, the Environmental Health Assessment Program (EHAP), conduct public health assessments to evaluate environmental data and community concerns. Contained within this PHA are the results of the Highway 36 Corridor Exposure Investigation (EI). The EI was conducted in response to resident’s concerns about potential exposures from pesticide applications occurring on forestlands near their homes and schools. At an April 2011 Board of Forestry meeting, several residents announced the results of a community- led, urine sampling effort. The results showed elevated levels of atrazine and 2,4-D in their urine. The Oregon Department of Forestry (ODF) notified the Pesticide Analytic and Response Center (PARC) of the results. As co-chair of PARC, OHA joined a multi-agency workgroup to develop the Highway 36 Corridor Exposure Investigation (EI) in order to determine if people are being exposed to pesticides in the Highway 36 corridor, and if so, the health implications of these exposures. For the purposes of this document, the following definitions apply: Public Health Assessment (PHA): A PHA is an evaluation tool of choice when a site contains multiple contaminants and multiple, potential pathways of exposure.
    [Show full text]
  • Common Name Or Designation Chemical Name Common And
    Common Name or Designation Chemical Name isouron (i-so- u-ron) N'-[5-(l,l-dimethylethyl)-3-isoxazolyl] -N,iV-dimethylurea isoxaben (i-'sok-a-ben) /V-[3-(l-ethyl-l-methylpropyl)-5-isoxazolyl] -2,6-dimethoxybenzamide karbutilate (kar- 'bu-ta-lat) 3-[[(dimethylamino)carbonylJ amino] phenyl (l,l-dimethylethyl)carbamate linuron (lin-u-ron) N'-(3,4-dichlorophenyl)-/V-methoxy-A/-methylurea MAA methylarsonic acid MAMA monoammonium salt of MAA MCPA (4-chloro-2-methylphenoxy)acetic acid MCPB 4-(4-chloro-2-methylphenoxy)butanoic acid mecoprop (Ynek-o-prop)^ (±)-2-(4-chloro-2-methylphenoxy)propanoic acid mefluidide (me- floo-i-did) N-[2,4-dimethyl-5-[ [(trifluoromethyl)sulfonyll amino] phenyl] acetamide metham ( meth-am) methylcarbamodithioic acid methazole ( meth-a-zol) 2-(3,4-dichlorophenyl)-4-methyl-l,2,4-oxadiazolidine-3,5-dione metolachlor (ma- tol-a-klor) 2-chloro-N-(2-ethyl-6-methylphenyl)-A/-(2-methoxy-l-methylethyl)acetamide metribuzin ('me-tri- 'bu-zan) 4-amino-6-(l,l-dimethylethyl)-3-(methylthio)-l,2,4-triazin-5(4H)-one metsulfuron (met-'sul-fu-ron) 2-[[ [ [(4-methoxy-6-methyl-l,3,5-triazin-2-yl)amino] carbonyl] amino] sulfonyl] benzoic acid MH l,2-dihydro-3,6-pyridazinedione molinate ( mo-lin-at) S-ethyl hexahydro-lH-azepine-l-carbothioate monuron TCA ( mon-u-ron) Salt of monuron and TCA MSMA monosodium salt of MAA napropamide (na-pro-pa-mid) /V,N-diethyl-2-(l-naphthalenyloxy)propanamide naptalam ('nap-ta-lam) 2-[(l-naphthalenylamino)carbonyl] benzoic acid nitrofen ( ni-tro-fen) 2,4-dichloro-l-(4-nitrophenqxy)benzene norea (nor- e-a)
    [Show full text]