UC Riverside UC Riverside Electronic Theses and Dissertations
Total Page:16
File Type:pdf, Size:1020Kb
Load more
Recommended publications
-
Acidic Phospholipids,47, 58 Acidification Steps, 23 Adsorbent, 53
Index acidic phospholipids,47, 58 ATPase, 22, 107 acidification steps, 23 auto-oxidation, 48,49 adsorbent, 53 aequorin, 95 aggregated mitochondria, 23 bacterial cells, 10 alcohols, 49 bacteriorhodopsin (BR), 218,232 alkaline hydrolysis, I bee venom, 121 alkyl esters, 57, 59 binders,53 amide bond, 129 silica gel, 53, 65, 176 amide linkage, 14 silica gel H, 176 amino alcohol, II bioactive phospholipids, 144 amino-group labelling reagents, 121 blanching, 50 aminopeptidase, 17 buoyant density, 16 aminophospholipid, 113, 118 butyl hydroxy toluene (BHT), 49,212 aminophospholipid pump, 119, 140 aminophospholipid translocase, 11 3 amphotericin B (AmB), 107 Cal+ -ATPases, 28, 107 N-(1-deoxyD-fructos-1-yl) AmB, 107 Cal+ -uptake, 27 anchored lipids, 38 campesterol, 104 angular amplitude, 89 calciferol, 78 anilino-8-naphthalene sulfonate (ANS), Candida albicans, 59, 60, 61, 76, 78, 102, 94, 103 104, 108 animal cells, 10 caproic acid, 129, 130 animal tissues, 9 carotenoids, 37 anion transporter, 135 cell debris, 33 anisotropy, 81 , 89, 95, 96, 97, 102, 104, cell disintegrator, 19 105 ceramide, 14, 129, 194 antagonists, 178 ceramide monohexosides ( CMH), 60 antioxidant, 49, 50 cerebroside, 14 apolar lipids, 56, 57, 69 chemical probes,112 arachidonic acid (AA), 144,153, 161 chloroplast, 32, 33 artificial membrane, 83 isolation, 32, 33 ascending chromatography, 54 purification, 33 asymmetric topology, 128 cholesterol, 14, 15,212 asymmetry, 112, 113,119 choline, 7, 9 atebrin, 95 chromatograms, 55 Index 249 chromatographic analyses, 52 ELISA, 146, 148 -
The Vascular Plants of Massachusetts
The Vascular Plants of Massachusetts: The Vascular Plants of Massachusetts: A County Checklist • First Revision Melissa Dow Cullina, Bryan Connolly, Bruce Sorrie and Paul Somers Somers Bruce Sorrie and Paul Connolly, Bryan Cullina, Melissa Dow Revision • First A County Checklist Plants of Massachusetts: Vascular The A County Checklist First Revision Melissa Dow Cullina, Bryan Connolly, Bruce Sorrie and Paul Somers Massachusetts Natural Heritage & Endangered Species Program Massachusetts Division of Fisheries and Wildlife Natural Heritage & Endangered Species Program The Natural Heritage & Endangered Species Program (NHESP), part of the Massachusetts Division of Fisheries and Wildlife, is one of the programs forming the Natural Heritage network. NHESP is responsible for the conservation and protection of hundreds of species that are not hunted, fished, trapped, or commercially harvested in the state. The Program's highest priority is protecting the 176 species of vertebrate and invertebrate animals and 259 species of native plants that are officially listed as Endangered, Threatened or of Special Concern in Massachusetts. Endangered species conservation in Massachusetts depends on you! A major source of funding for the protection of rare and endangered species comes from voluntary donations on state income tax forms. Contributions go to the Natural Heritage & Endangered Species Fund, which provides a portion of the operating budget for the Natural Heritage & Endangered Species Program. NHESP protects rare species through biological inventory, -
Bioluminescent Properties of Semi-Synthetic Obelin and Aequorin Activated by Coelenterazine Analogues with Modifications of C-2, C-6, and C-8 Substituents
International Journal of Molecular Sciences Article Bioluminescent Properties of Semi-Synthetic Obelin and Aequorin Activated by Coelenterazine Analogues with Modifications of C-2, C-6, and C-8 Substituents 1, 2,3, 1 2, Elena V. Eremeeva y , Tianyu Jiang y , Natalia P. Malikova , Minyong Li * and Eugene S. Vysotski 1,* 1 Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk 660036, Russia; [email protected] (E.V.E.); [email protected] (N.P.M.) 2 Key Laboratory of Chemical Biology (MOE), Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China; [email protected] 3 State Key Laboratory of Microbial Technology, Shandong University–Helmholtz Institute of Biotechnology, Shandong University, Qingdao 266237, China * Correspondence: [email protected] (M.L.); [email protected] (E.S.V.); Tel.: +86-531-8838-2076 (M.L.); +7-(391)-249-44-30 (E.S.V.); Fax: +86-531-8838-2076 (M.L.); +7-(391)-290-54-90 (E.S.V.) These authors contributed equally to this work. y Received: 23 June 2020; Accepted: 27 July 2020; Published: 30 July 2020 Abstract: Ca2+-regulated photoproteins responsible for bioluminescence of a variety of marine organisms are single-chain globular proteins within the inner cavity of which the oxygenated coelenterazine, 2-hydroperoxycoelenterazine, is tightly bound. Alongside with native coelenterazine, photoproteins can also use its synthetic analogues as substrates to produce flash-type bioluminescence. However, information on the effect of modifications of various groups of coelenterazine and amino acid environment of the protein active site on the bioluminescent properties of the corresponding semi-synthetic photoproteins is fragmentary and often controversial. -
Bioluminescence Related Publications from JNC Corporation
Bioluminescence related publications since 1985 110) Inouye, S. and Hojo, H. (2018) Revalidation of recombinant aequorin as a light emission standard: Estimation of specific activity of Gaussia luciferase. Biochem. Biophys. Res. Commun. 507: 242-245. 109) Yokawa, S., Suzuki, T., Hayashi, A., Inouye, S., Inoh, Y. and Furuno, T. (2018) Video-rate bioluminescence imaging of degranulation of mast cells attached to the extracellular matrix. Front. Cell Dev. Biol. 6: 74. 108) Inouye, S., Tomabechi, Y., Hosoya, T., Sekine, S. and Shirouzu, M. (2018) Slow luminescence kinetics of semi-synthetic aequorin:expression, purification and structure determination of cf3-aequorin. J. Biochem. 164(3): 247–255. 107) Inouye, S. (2018) Single-step purification of recombinant Gaussia luciferase from serum-containing culture medium of mammalian cells. Protein Expr. Purif. 141: 32-38. 106) Inouye, S. and Sahara-Miura, Y. (2017) A fusion protein of the synthetic IgG-binding domain and aequorin: Expression and purification from E. coli cells and its application. Protein Expr. Purif. 137: 58-63. 105) Suzuki, T., Kanamori, T. and Inouye, S. (2017) Quantitative visualization of synchronized insulin secretion from 3D-cultured cells. Biochem. Biophys. Res. Commun. 486: 886-892. 104) Yokawa, S., Suzuki, T., Inouye, S., Inoh, Y., Suzuki, R., Kanamori, T., Furuno, T. and Hirashima, N. (2017) Visualization of glucagon secretion from pancreatic α cells by bioluminescence video microscopy: Identification of secretion sites in the intercellular contact regions. Biochem. Biophys. Res. Commun. 485: 725-730. 103) Inouye, S. and Suzuki, T. (2016) Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells. -
BPS Complete Catalog
BPSCATALOG ENZYMES KITS CELL LINES SCREENING SERVICES INNOVATIVE PRODUCTS TO FUEL YOUR EXPERIMENTS Unique, expert portfolio 2017 OUR MISSION: To provide the highest quality life science products and services worldwide in a timely manner to assist in accelerating drug discovery research and development for the treatment of human diseases. INNOVATION WE CONTINUOUSLY STRIVE TO BE FIRST TO MARKET BY PROVIDING EXPERTISE IN EXPRESSING HIGHLY ACTIVE ENZYMES MULTIPLE ASSAY DETECTION FORMATS HUMAN, MURINE AND MONKEY VERSIONS OF RECOMBINANT PROTEINS BIOTINYLATED VERSIONS OF MANY IMMUNE CHECKPOINT RECEPTORS 1ST AND MOST EXTENSIVE PARP ISOZYME PORTFOLIO 1ST AND MOST EXTENSIVE PDE ISOZYME PORTFOLIO 1ST COMPLETE SUITE OF HDAC AND SIRT ENZYMES 1ST AVAILABLE PARP AND TANKYRASE PROFILING SERVICES 1ST COMMERCIALLY AVAILABLE HISTONE DEMETHYLASES LARGEST OFFERING OF METHYLTRANSFERASES LARGEST OFFERING OF DEMETHYLASES OVER 200 PRODUCTS AND SERVICES EXCLUSIVE TO BPS RELIABILITY BPS IS CITED IN PEER-REVIEWED JOURNALS AND PUBLICATIONS AROUND THE WORLD NATURE GENETICS THE JOURNAL OF BIOLOGICAL CHEMISTRY JOURNAL OF CELL SCIENCE ANALYTICAL BIOCHEMISTRY BBRC NATURE CHEMICAL BIOLOGY ACS MEDICINAL CHEMISTRY LETTERS JOURNAL OF NATURAL PRODUCTS CHEMMEDCHEM MOLECULAR CANCER THERAPEUTICS & MANY MORE TABLE OF CONTENTS ACETYLTRANSFERASE 4 APOPTOSIS 4-5 ANTIBODIES 6-9 BIOTINYLATION 10-11 BROMODOMAINS 12-14 CELL BASED ASSAY KITS 15 CELL LINES 16-17 CELL SURFACE RECEPTORS 18-20 CYTOKINE(S) 21-24 DEACETYLASE(S) 25-27 DEMETHYLASE(S) 28-29 HEAT SHOCK PROTEINS 30 IMMUNOTHERAPY -
Neobeckia Aquatica Eaton (Greene) North American Lake Cress
New England Plant Conservation Program Conservation and Research Plan Neobeckia aquatica Eaton (Greene) North American Lake Cress Prepared by: John D. Gabel and Donald H. Les University of Connecticut Storrs, Connecticut For: New England Wild Flower Society 180 Hemenway Road Framingham, MA 01701 508/877-7630 e-mail: [email protected] ! website: www.newfs.org Approved, Regional Advisory Council, 2000 SUMMARY The North American lake cress, Neobeckia aquatica (Eaton) Greene (Brassicaceae), is listed as S1 in Vermont, SH in Massachusetts, and “SH?” in Maine. Lake cress likely requires clear, slow-moving water. A requirement of sites is that they have regular fluctuations in water level. Sites are typically located in gently flowing riverine systems and have little or no shoreline development. Special threats include invasive plant species, eutrophication, and development of habitat. All extant New England element occurrences of lake cress are located in Vermont at four sites. VT.002, Orwell is characterized by small population numbers (two to five plants). The site is highly eutrophic and threatened by invasive aquatic plants (Butomus umbellatus, Lythrum salicaria, and Trapa natans). VT.006, Orwell is characterized by a relatively large population (100-500 plants). The site is threatened by invasive aquatic plants (Butomus umbellatus , Lythrum salicaria, and Trapa natans.) VT.009, Shoreham is a highly eutrophic site with 500-1000 plants in the population. VT.010, Isle La Motte represents a population located in a pristine habitat with around 500 plants. The conservation objectives for Neobeckia aquatica in New England are to: C remove the threat of invasive plants from extant lake cress populations. -
Platelet-Neutrophil Interaction
PLATELET-NEUTROPHIL INTERACTION: NEUTROPHILS MODULATE PLATELET FUNCTION Richard William FAINT 1992 A thesis submitted to the University of London for the degree of Ph.D The Department of Haematology University College and Middlesex School of Medicine University College London 98 Chenies Mews London WC1E 6HX ProQuest Number: U049250 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a com plete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest U049250 Published by ProQuest LLC(2017). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States C ode Microform Edition © ProQuest LLC. ProQuest LLC. 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106- 1346 2 ABSTRACT Platelets are vital for the arrest of bleeding following tissue injury. Neutrophils play an important role in host defence against bacterial infection. In the milieu of the acute inflammatory response both cellular and non-cellular elements may interact to modify behaviour. Evidence suggests that leukocytes may play an active role in the modulation of platelet function. This interaction may be abnormal in certain pathological states. This study examined the effect of purified neutrophils upon both washed platelets and platelet-rich plasma, as well as in whole blood in vitro. Neutrophils were found to alter platelet behaviour by several mechanisms. These included transcellular metabolism of eicosanoids. -
Summary Report of Nonindigenous Aquatic Species in U.S. Fish and Wildlife Service Region 5
Summary Report of Nonindigenous Aquatic Species in U.S. Fish and Wildlife Service Region 5 Summary Report of Nonindigenous Aquatic Species in U.S. Fish and Wildlife Service Region 5 Prepared by: Amy J. Benson, Colette C. Jacono, Pam L. Fuller, Elizabeth R. McKercher, U.S. Geological Survey 7920 NW 71st Street Gainesville, Florida 32653 and Myriah M. Richerson Johnson Controls World Services, Inc. 7315 North Atlantic Avenue Cape Canaveral, FL 32920 Prepared for: U.S. Fish and Wildlife Service 4401 North Fairfax Drive Arlington, VA 22203 29 February 2004 Table of Contents Introduction ……………………………………………………………………………... ...1 Aquatic Macrophytes ………………………………………………………………….. ... 2 Submersed Plants ………...………………………………………………........... 7 Emergent Plants ………………………………………………………….......... 13 Floating Plants ………………………………………………………………..... 24 Fishes ...…………….…………………………………………………………………..... 29 Invertebrates…………………………………………………………………………...... 56 Mollusks …………………………………………………………………………. 57 Bivalves …………….………………………………………………........ 57 Gastropods ……………………………………………………………... 63 Nudibranchs ………………………………………………………......... 68 Crustaceans …………………………………………………………………..... 69 Amphipods …………………………………………………………….... 69 Cladocerans …………………………………………………………..... 70 Copepods ……………………………………………………………….. 71 Crabs …………………………………………………………………...... 72 Crayfish ………………………………………………………………….. 73 Isopods ………………………………………………………………...... 75 Shrimp ………………………………………………………………….... 75 Amphibians and Reptiles …………………………………………………………….. 76 Amphibians ……………………………………………………………….......... 81 Toads and Frogs -
Antibody List
產品編號 產品名稱 PA569955 1110059E24Rik Polyclonal Antibody PA569956 1110059E24Rik Polyclonal Antibody PA570131 1190002N15Rik Polyclonal Antibody 01-1234-42 123count eBeads Counting Beads MA512242 14.3.3 Pan Monoclonal Antibody (CG15) LFMA0074 14-3-3 beta Monoclonal Antibody (60C10) LFPA0077 14-3-3 beta Polyclonal Antibody PA137002 14-3-3 beta Polyclonal Antibody PA14647 14-3-3 beta Polyclonal Antibody PA515477 14-3-3 beta Polyclonal Antibody PA517425 14-3-3 beta Polyclonal Antibody PA522264 14-3-3 beta Polyclonal Antibody PA529689 14-3-3 beta Polyclonal Antibody MA134561 14-3-3 beta/epsilon/zeta Monoclonal Antibody (3C8) MA125492 14-3-3 beta/zeta Monoclonal Antibody (22-IID8B) MA125665 14-3-3 beta/zeta Monoclonal Antibody (4E2) 702477 14-3-3 delta/zeta Antibody (1H9L19), ABfinity Rabbit Monoclonal 711507 14-3-3 delta/zeta Antibody (1HCLC), ABfinity Rabbit Oligoclonal 702241 14-3-3 epsilon Antibody (5H10L5), ABfinity Rabbit Monoclonal 711273 14-3-3 epsilon Antibody (5HCLC), ABfinity Rabbit Oligoclonal PA517104 14-3-3 epsilon Polyclonal Antibody PA528937 14-3-3 epsilon Polyclonal Antibody PA529773 14-3-3 epsilon Polyclonal Antibody PA575298 14-3-3 eta (Lys81) Polyclonal Antibody MA524792 14-3-3 eta Monoclonal Antibody PA528113 14-3-3 eta Polyclonal Antibody PA529774 14-3-3 eta Polyclonal Antibody PA546811 14-3-3 eta Polyclonal Antibody MA116588 14-3-3 gamma Monoclonal Antibody (HS23) MA116587 14-3-3 gamma Monoclonal Antibody (KC21) PA529690 14-3-3 gamma Polyclonal Antibody PA578233 14-3-3 gamma Polyclonal Antibody 510700 14-3-3 Pan Polyclonal -
Arbuscular Mycorrhizal Fungi and Dark Septate Fungi in Plants Associated with Aquatic Environments Doi: 10.1590/0102-33062016Abb0296
Arbuscular mycorrhizal fungi and dark septate fungi in plants associated with aquatic environments doi: 10.1590/0102-33062016abb0296 Table S1. Presence of arbuscular mycorrhizal fungi (AMF) and/or dark septate fungi (DSF) in non-flowering plants and angiosperms, according to data from 62 papers. A: arbuscule; V: vesicle; H: intraradical hyphae; % COL: percentage of colonization. MYCORRHIZAL SPECIES AMF STRUCTURES % AMF COL AMF REFERENCES DSF DSF REFERENCES LYCOPODIOPHYTA1 Isoetales Isoetaceae Isoetes coromandelina L. A, V, H 43 38; 39 Isoetes echinospora Durieu A, V, H 1.9-14.5 50 + 50 Isoetes kirkii A. Braun not informed not informed 13 Isoetes lacustris L.* A, V, H 25-50 50; 61 + 50 Lycopodiales Lycopodiaceae Lycopodiella inundata (L.) Holub A, V 0-18 22 + 22 MONILOPHYTA2 Equisetales Equisetaceae Equisetum arvense L. A, V 2-28 15; 19; 52; 60 + 60 Osmundales Osmundaceae Osmunda cinnamomea L. A, V 10 14 Salviniales Marsileaceae Marsilea quadrifolia L.* V, H not informed 19;38 Salviniaceae Azolla pinnata R. Br.* not informed not informed 19 Salvinia cucullata Roxb* not informed 21 4; 19 Salvinia natans Pursh V, H not informed 38 Polipodiales Dryopteridaceae Polystichum lepidocaulon (Hook.) J. Sm. A, V not informed 30 Davalliaceae Davallia mariesii T. Moore ex Baker A not informed 30 Onocleaceae Matteuccia struthiopteris (L.) Tod. A not informed 30 Onoclea sensibilis L. A, V 10-70 14; 60 + 60 Pteridaceae Acrostichum aureum L. A, V, H 27-69 42; 55 Adiantum pedatum L. A not informed 30 Aleuritopteris argentea (S. G. Gmel) Fée A, V not informed 30 Pteris cretica L. A not informed 30 Pteris multifida Poir. -
Site-Specific Mutagenesis of the Calcium-Binding Photoprotein
Proc. Natd. Acad. Sci. USA Vol. 83, pp. 8107-8111, November 1986 Biochemistry Site-specific mutagenesis of the calcium-binding photoprotein aequorin (bioluninescence/oxygenase/oligonudeotide-directed mutagenesis/protein modification) FREDERICK I. TsuJI*, SATOSHI INOUYEtt, TOSHIO GOTO§, AND YOSHIYUKI SAKAKIt *Marine Biology Research Division, Scripps Institution of Oceanography, University of California-San Diego, La Jolla, CA 92093, and V. A. Medical Center Brentwood, Los Angeles, CA 90073; tResearch Laboratory for Genetic Information, Kyushu University, Fukuoka 812, Japan; and §Department of Agricultural Chemistry, Faculty of Agriculture, Nagoya University, Nagoya 464, Japan Communicated by Martin D. Kamen, July 21, 1986 ABSTRACT The luminescent protein aequorin from the which amino acid substitutions were made at the three jellyfish Aequoria victoria emits light by an intramolecular Ca2l-binding sites, at the three cysteines, and at histidine in reaction in the presence of a trace amount of Ca2 . In order to the hydrophobic region. understand the mechanism of the reaction, a study of structure-function relationships was undertaken with respect MATERIALS AND METHODS to modifying certain of its amino acid residues. This was done by carrying out oligonudeotide-directed site-specific mutagen- Enzymes and Chemicals. All restriction endonucleases, esis of apoaequorin cDNA and expressing the mutagenized Escherichia coli T4 DNA ligase, Klenow enzyme of DNA cDNA in Escherichia cofi. Amino acid substitutions were made polymerase 1, and T4 polynucleotide kinase were purchased at the three Ca2+-binding sites, the three cysteines, and a from Takara Shuzo (Kyoto, Japan). Tris, 2-mercaptoethanol, histidine in one of the hydrophobic regions. Subsequent assay and isopropyl-,fD-thiogalactopyranoside were obtained from of the modified aequorin showed that the Ca2 -binding sites, Wako Pure Chemicals (Osaka, Japan). -
Chimeric Green Fluorescent Protein-Aequorin As Bioluminescent Ca2؉ Reporters at the Single-Cell Level
Chimeric green fluorescent protein-aequorin as bioluminescent Ca2؉ reporters at the single-cell level Vale´ rie Baubet*, Herve´ Le Mouellic*, Anthony K. Campbell†, Estelle Lucas-Meunier‡, Philippe Fossier‡, and Philippe Bruˆlet*§ *Unite´d’Embryologie Mole´culaire, Unite´de Recherche Associe´e 1947, Centre National de la Recherche Scientifique, Institut Pasteur, 25 rue du docteur Roux, 75724 Paris Cedex 15, France; †Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF4 4XN, United Kingdom; and ‡Laboratoire de Neurobiologie Cellulaire et Mole´culaire, UPR 9040 Centre National de la Recherche Scientifique, avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France Communicated by Franc¸ois Jacob, Institut Pasteur, Paris, France, April 14, 2000 (received for review February 1, 2000) 2ϩ ϭ Monitoring calcium fluxes in real time could help to understand the Its low affinity for Ca (Kd 10 M) is probably responsible development, the plasticity, and the functioning of the central for this and makes aequorin a good sensor in the range of nervous system. In jellyfish, the chemiluminescent calcium binding biological Ca2ϩ concentration variations. Although providing a aequorin protein is associated with the green fluorescent protein good ratio of signal over background, aequorin signals are very ,and a green bioluminescent signal is emitted upon Ca2؉ stimula- difficult to detect because of aequorin’s low light quantum yield tion. We decided to use this chemiluminescence resonance energy that is the number of emitted photons per protein that bind Ca2ϩ. transfer between the two molecules. Calcium-sensitive biolumi- In the jellyfish Aequorea victoria from which aequorin has been nescent reporter genes have been constructed by fusing green isolated (10), the protein is associated with the GFP (11).