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(12) United States Patent (10) Patent No.: US 8,148,067 B2 Lal Et Al

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(12) United States Patent (10) Patent No.: US 8,148,067 B2 Lal Et Al US008148067B2 (12) United States Patent (10) Patent No.: US 8,148,067 B2 Lal et al. (45) Date of Patent: Apr. 3, 2012 (54) METHODS FOR DIAGNOSING AND 5,219,727 A 6/1993 Wang et al. MONITORING THE STATUS OF SYSTEMC 5,264,351 A 1 1/1993 Harley 5,278,043 A 1/1994 Bannwarth et al. LUPUSERYTHEMATOSUS 5,310,652 A 5/1994 Gelfand et al. 5,314,809 A 5/1994 Erlich et al. (75) Inventors: Preeti G. Lal, Santa Clara, CA (US); 5,322,770 A 6/1994 Gelfand 5,340,720 A 8, 1994 Stetler Gavin E. Williams, Menlo Park, CA 5,346,994 A 9/1994 Chomczynski (US); Kirk E. Fry, Palo Alto, CA (US); 5,352,600 A 10, 1994 Gelfand et al. Jingtao Sun, Foster City, CA (US); 5,374,553 A 12/1994 Gelfand et al. Russel L. Dedrick, Kensington, CA 5,385,824 A 1/1995 Hoet et al. (US) 5,389,512 A 2/1995 Sninsky et al. 5,393,672 A 2f1995 Van Ness et al. 5,405,774 A 4, 1995 Abramson et al. 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Primary Examiner — James Martinell 4,800,159 A 1, 1989 Mullis et al. 4,818,418 A 4, 1989 Saunders (74) Attorney, Agent, or Firm — Morrison & Foerster LLP 4,843,155 A 6/1989 Chomczynski 4,889,818 A 12/1989 Gelfand et al. 4,908,318 A 3, 1990 Lerner (57) ABSTRACT 4.946,778 A 8, 1990 Ladner et al. 4.946,952 A 8, 1990 Kiefer The invention presents a method of diagnosing or monitoring 4,965,188 A 10, 1990 Mullis et al. the status of systemic lupus erythematosus (SLE) in a subject 5,053,134 A 10, 1991 Luderer et al. or patient comprising detecting the expression of all genes of 5,063,162 A 11, 1991 Kiefer 5,066,584 A 1 1/1991 Gyllensten et al. a diagnostic set in the Subject or patient wherein the diagnos 5,075.216 A 12/1991 Innis et al. tic set comprises two or more genes having expression cor 5,079,352 A 1/1992 Gelfand et al. related with the classification or status of SLE; and diagnos 5,091,310 A 2f1992 Innis ing or monitoring the status of SLE in the Subject or patient by 5, 120,525 A 6/1992 Goldenberg 5,142,033. 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