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Development and Evaluation of Rrna Targeted in Situ Probes and Phylogenetic Relationships of Freshwater Fungi
Development and evaluation of rRNA targeted in situ probes and phylogenetic relationships of freshwater fungi vorgelegt von Diplom-Biologin Christiane Baschien aus Berlin Von der Fakultät III - Prozesswissenschaften der Technischen Universität Berlin zur Erlangung des akademischen Grades Doktorin der Naturwissenschaften - Dr. rer. nat. - genehmigte Dissertation Promotionsausschuss: Vorsitzender: Prof. Dr. sc. techn. Lutz-Günter Fleischer Berichter: Prof. Dr. rer. nat. Ulrich Szewzyk Berichter: Prof. Dr. rer. nat. Felix Bärlocher Berichter: Dr. habil. Werner Manz Tag der wissenschaftlichen Aussprache: 19.05.2003 Berlin 2003 D83 Table of contents INTRODUCTION ..................................................................................................................................... 1 MATERIAL AND METHODS .................................................................................................................. 8 1. Used organisms ............................................................................................................................. 8 2. Media, culture conditions, maintenance of cultures and harvest procedure.................................. 9 2.1. Culture media........................................................................................................................... 9 2.2. Culture conditions .................................................................................................................. 10 2.3. Maintenance of cultures.........................................................................................................10 -
Boletus Mushrooms La Tia Jackson, Ian C
Genetic Diversity within Alaskan Boletus Mushrooms www.fungi-zette.com La Tia Jackson, Ian C. Herriott, József Geml, Gary A. Laursen, D. Lee Taylor Discussion Abstract •Clade 1 is composed of Alaskan samples from the interior and Northwest and We analyzed the genetic differences within the collection of samples from Europe. Although the species names do not match up they are genetically very similar, and B. citrinovirens is thought to be in what is called the Boletus mushroom from the UAF Fungal Herbarium, representing Identification on Tree Location subtomentosus group. samples from all over Alaska. Upon analyzing the DNA sequence Results DQ066405_Boletus_citrinovirens Europe from 18 samples, we found that most Alaskan Boletus are closely DQ066407_Boletus_spadiceus Europe •Clade 2 is composed of Alaskan samples from Southeast Alaska and GenBank DQ066397_Boletus_citrinovirens Europe samples from Europe, both identified as Boletus mirabilis. related to samples from the same species found in other parts of the world (clades 1,2,5,6,8,12). We also found that some species DQ066410_Boletus_spadiceus Europe •Clade 3 is composed of samples only from Alaska. None of the sequences DQ384578_Boletellus_mirabilis Europe collected in other parts of the world were not found in our sample 1 mycorance.free.fr obtained from GenBank are closely related. These were morphologically collection (clades 4,7,9,10,11). Finally, our results suggested that AJ419187_Boletus_impolitus Spain identified as B. subglabripes. “B. subglabripes” is in GenBank, but only Large 100 DQ131632_Xerocomus_subtomentosus Europe clade 3 on our phylogenetic tree is not represented among the Subunit gene, not Internally Transcribed Spacer gene (reference 2) so comparison AJ889931_Boletus_pruinatus Europe GenBank sequences from other parts of the world and may be a 100 is not possible to determine if morphological species attribution is the same or 2 AM087271_Xerocomus_pruinatus Europe genetic lineage endemic to Alaska. -
The Contribution of DNA Metabarcoding
The Contribution of DNA Metabarcoding to Fungal Conservation: Diversity Assessment, Habitat Partitioning and Mapping Red-Listed Fungi in Protected Coastal Salix repens Communities in the Netherlands Jo´ zsef Geml1,2*, Barbara Gravendeel1,2,3, Kristiaan J. van der Gaag4, Manon Neilen1, Youri Lammers1, Niels Raes1, Tatiana A. Semenova1,2, Peter de Knijff4, Machiel E. Noordeloos1 1 Naturalis Biodiversity Center, Leiden, The Netherlands, 2 Faculty of Science, Leiden University, Leiden, The Netherlands, 3 University of Applied Sciences Leiden, Leiden, The Netherlands, 4 Forensic Laboratory for DNA Research, Human Genetics, Leiden University Medical Centre, Leiden, The Netherlands Abstract Western European coastal sand dunes are highly important for nature conservation. Communities of the creeping willow (Salix repens) represent one of the most characteristic and diverse vegetation types in the dunes. We report here the results of the first kingdom-wide fungal diversity assessment in S. repens coastal dune vegetation. We carried out massively parallel pyrosequencing of ITS rDNA from soil samples taken at ten sites in an extended area of joined nature reserves located along the North Sea coast of the Netherlands, representing habitats with varying soil pH and moisture levels. Fungal communities in Salix repens beds are highly diverse and we detected 1211 non-singleton fungal 97% sequence similarity OTUs after analyzing 688,434 ITS2 rDNA sequences. Our comparison along a north-south transect indicated strong correlation between soil pH and fungal community composition. The total fungal richness and the number OTUs of most fungal taxonomic groups negatively correlated with higher soil pH, with some exceptions. With regard to ecological groups, dark-septate endophytic fungi were more diverse in acidic soils, ectomycorrhizal fungi were represented by more OTUs in calcareous sites, while detected arbuscular mycorrhizal genera fungi showed opposing trends regarding pH. -
SOMA News March 2011
VOLUME 23 ISSUE 7 March 2011 SOMA IS AN EDUCATIONAL ORGANIZATION DEDICATED TO MYCOLOGY. WE ENCOURAGE ENVIRONMENTAL AWARENESS BY SHARING OUR ENTHUSIASM THROUGH PUBLIC PARTICIPATION AND GUIDED FORAYS. WINTER/SPRING 2011 SPEAKER OF THE MONTH SEASON CALENDAR March Connie and Patrick March 17th » Meeting—7pm —“A Show and Tell”— Sonoma County Farm Bureau Speaker: Connie Green & Patrick March 17th—7pm Hamilton Foray March. 19th » Salt Point April April 21st » Meeting—7pm Sonoma County Farm Bureau Speaker: Langdon Cook Foray April 23rd » Salt Point May May 19th » Meeting—7pm Sonoma County Farm Bureau Speaker: Bob Cummings Foray May: Possible Morel Camping! eparated at birth but from the same litter Connie Green and Patrick Hamilton have S traveled (endured?) mushroom journeys together for almost two decades. They’ve been to the humid and hot jaguar jungles of Chiapas chasing tropical mushrooms and to EMERGENCY the cloud forests of the Sierra Madre for boletes and Indigo milky caps. In the cold and wet wilds of Alaska they hiked a spruce and hemlock forest trail to watch grizzly bears MUSHROOM tearing salmon bellies just a few yards away. POISONING IDENTIFICATION In the remote Queen Charlotte Islands their bush plane flew over “fields of golden chanterelles,” landed on the ocean, and then off into a zany Zodiac for a ride over a cold After seeking medical attention, contact and roiling sea alongside some low flying puffins to the World Heritage Site of Ninstints. Darvin DeShazer for identification at The two of them have gazed at glaciers and berry picked on muskeg bogs. More than a (707) 829-0596. -
Revista Mexicana De Ingeniería Química
Vol. 19, No. 3 (2020) 1277-1290 Revista Mexicana de Ingeniería Química Hypomyces chrysospermus ACL-01 isolatedCONTENIDO from Boletus edulis and its effect against fungal Volumen 8, número 3, 2009cereal / Volume pathogens 8, number 3, 2009 Hypomyces chrysospermus ACL-01 aislado a partir de Boletus edulis y su efecto sobre hongos patógenos de cereales 213 Derivation and application of the Stefan-Maxwell equations A. Cartagena-Luna1, A.R. Gayosso-Mexia1, M.A. Anducho-Reyes1, E.O. López-Villegas2, Y. Mercado-Flores1* 1Laboratorio de Agrobiotecnología. (Desarrollo y Universidad aplicación de Politécnica las ecuaciones de Pachuca.de Stefan-Maxwell) Carretera Pachuca-Cd. Sahagún Km 20 Ex-Hacienda Stephen Whitakerde Santa Bárbara, 43830, Zempoala Hidalgo, México. 2 Central de Microscopía. Escuela Nacional de Ciencias Biológicas. Instituto Politécnico Nacional. Prolongación de Carpio y Plan de Ayala S/N, Santo Tomás, 11340, Miguel Hidalgo, CDMX, México. Biotecnología / Biotechnology Received: August 16, 2019; Accepted: January 17, 2020 245 Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo Abstract intemperizados en suelos y sedimentos In this study, the Hypomyces chrysospermus ACL-01 fungus was isolated from the fruiting body of edible basidiomycete Boletus edulis, collected in Acaxochitlán (Biodegradation in the modeling State of of Hidalgo, sludge bioreactors Mexico popularly of total petroleum known as hydrocarbons tlacoayel. Theweathering antagonistic in soil effect of the isolated fungus againstand di sediments)fferent fungal cereal pathogens was evaluated. The dual confrontation test and scanning electron microscopy analysis revealed that this ascomycete grows on the basidiomycetes Sporisorium reilianum and Tilletia sp., causing loss of cell viability. However,S.A. -
Influence of Tree Species on Richness and Diversity of Epigeous Fungal
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Archive Ouverte en Sciences de l'Information et de la Communication fungal ecology 4 (2011) 22e31 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/funeco Influence of tree species on richness and diversity of epigeous fungal communities in a French temperate forest stand Marc BUE´Ea,*, Jean-Paul MAURICEb, Bernd ZELLERc, Sitraka ANDRIANARISOAc, Jacques RANGERc,Re´gis COURTECUISSEd, Benoıˆt MARC¸AISa, Franc¸ois LE TACONa aINRA Nancy, UMR INRA/UHP 1136 Interactions Arbres/Microorganismes, 54280 Champenoux, France bGroupe Mycologique Vosgien, 18 bis, place des Cordeliers, 88300 Neufchaˆteau, France cINRA Nancy, UR 1138 Bioge´ochimie des Ecosyste`mes Forestiers, 54280 Champenoux, France dUniversite´ de Lille, Faculte´ de Pharmacie, F59006 Lille, France article info abstract Article history: Epigeous saprotrophic and ectomycorrhizal (ECM) fungal sporocarps were assessed during Received 30 September 2009 7 yr in a French temperate experimental forest site with six 30-year-old mono-specific Revision received 10 May 2010 plantations (four coniferous and two hardwood plantations) and one 150-year-old native Accepted 21 July 2010 mixed deciduous forest. A total of 331 fungal species were identified. Half of the fungal Available online 6 October 2010 species were ECM, but this proportion varied slightly by forest composition. The replace- Corresponding editor: Anne Pringle ment of the native forest by mono-specific plantations, including native species such as beech and oak, considerably altered the diversity of epigeous ECM and saprotrophic fungi. Keywords: Among the six mono-specific stands, fungal diversity was the highest in Nordmann fir and Conifer plantation Norway spruce plantations and the lowest in Corsican pine and Douglas fir plantations. -
CZECH MYCOLOGY Publication of the Czech Scientific Society for Mycology
CZECH MYCOLOGY Publication of the Czech Scientific Society for Mycology Volume 57 August 2005 Number 1-2 Central European genera of the Boletaceae and Suillaceae, with notes on their anatomical characters Jo s e f Š u t a r a Prosetická 239, 415 01 Tbplice, Czech Republic Šutara J. (2005): Central European genera of the Boletaceae and Suillaceae, with notes on their anatomical characters. - Czech Mycol. 57: 1-50. A taxonomic survey of Central European genera of the families Boletaceae and Suillaceae with tubular hymenophores, including the lamellate Phylloporus, is presented. Questions concerning the delimitation of the bolete genera are discussed. Descriptions and keys to the families and genera are based predominantly on anatomical characters of the carpophores. Attention is also paid to peripheral layers of stipe tissue, whose anatomical structure has not been sufficiently studied. The study of these layers, above all of the caulohymenium and the lateral stipe stratum, can provide information important for a better understanding of relationships between taxonomic groups in these families. The presence (or absence) of the caulohymenium with spore-bearing caulobasidia on the stipe surface is here considered as a significant ge neric character of boletes. A new combination, Pseudoboletus astraeicola (Imazeki) Šutara, is proposed. Key words: Boletaceae, Suillaceae, generic taxonomy, anatomical characters. Šutara J. (2005): Středoevropské rody čeledí Boletaceae a Suillaceae, s poznámka mi k jejich anatomickým znakům. - Czech Mycol. 57: 1-50. Je předložen taxonomický přehled středoevropských rodů čeledí Boletaceae a. SuiUaceae s rourko- vitým hymenoforem, včetně rodu Phylloporus s lupeny. Jsou diskutovány otázky týkající se vymezení hřibovitých rodů. Popisy a klíče k čeledím a rodům jsou založeny převážně na anatomických znacích plodnic. -
Four Newly Recorded Amanita Taxa from India
BIODIVERSITAS ISSN: 1412-033X Volume 17, Number 1, April 2016 E-ISSN: 2085-4722 Pages: 342-348 DOI: 10.13057/biodiv/d170146 Four newly recorded Amanita taxa from India YADWINDER SINGH♥, MUNRUCHI KAUR Department of Botany, Punjabi University, University College of Engineering Rd, Urban Estate Phase II, Patiala-147002, Punjab, India. Tel.::+91 175 304 6265, email: [email protected] Manuscript received: 9 March 2015. Revision accepted: 16 April 2016. Abstract. Singh Y, Kaur M. 2016. Four newly recorded Amanita taxa from India. Biodiversitas 17: 342-348. During the fungal forays in district Himachal Pradesh of North Western India, four unrecorded taxa of Amanita were collected. They are Amanita flavoconia var. flavoconia, A. flavoconia var. inquinata, A. pilosella f. pilosella and A. porphyria. A. flavoconia var. flavoconia is distinctive in having a brilliant yellow to yellow orange cap, with white lamellae and a stipe base turning light brownish on injury. Whereas. A. flavoconia var. inquinata possesses brownish orange to yellowish orange to orange yellow, subvicid pileus, having white to pastel yellow stipe, annulus grayish yellow, superior and volva forming broken rings of yellow patches around the bulb. A. pilosella f. pilosella unique in possessing grayish brown or brownish beige pileus having thick irregular warts and white lamellae edges. A. porphyria is represented by nonstriate pileus margin, off white stipe, decorated with grayish squamules, with violaceous tinge and has a marginate bulb, annulus is persistent, off white above and light grey below and volva is friable, as grey, cottony mass at the margin of the bulb. Keywords: Amanitaceae, new record, India, taxonomy INTRODUCTION microscope (Olympus) on 100x and 40x by cutting free hand sections of revived part of the dried specimen and The genus Amanita belongs to the family Amanitaceae staining them in 1% cotton blue or 2% congo red. -
Forest Fungi in Ireland
FOREST FUNGI IN IRELAND PAUL DOWDING and LOUIS SMITH COFORD, National Council for Forest Research and Development Arena House Arena Road Sandyford Dublin 18 Ireland Tel: + 353 1 2130725 Fax: + 353 1 2130611 © COFORD 2008 First published in 2008 by COFORD, National Council for Forest Research and Development, Dublin, Ireland. All rights reserved. No part of this publication may be reproduced, or stored in a retrieval system or transmitted in any form or by any means, electronic, electrostatic, magnetic tape, mechanical, photocopying recording or otherwise, without prior permission in writing from COFORD. All photographs and illustrations are the copyright of the authors unless otherwise indicated. ISBN 1 902696 62 X Title: Forest fungi in Ireland. Authors: Paul Dowding and Louis Smith Citation: Dowding, P. and Smith, L. 2008. Forest fungi in Ireland. COFORD, Dublin. The views and opinions expressed in this publication belong to the authors alone and do not necessarily reflect those of COFORD. i CONTENTS Foreword..................................................................................................................v Réamhfhocal...........................................................................................................vi Preface ....................................................................................................................vii Réamhrá................................................................................................................viii Acknowledgements...............................................................................................ix -
And Interspecific Hybridiation in Agaric Fungi
Mycologia, 105(6), 2013, pp. 1577–1594. DOI: 10.3852/13-041 # 2013 by The Mycological Society of America, Lawrence, KS 66044-8897 Evolutionary consequences of putative intra- and interspecific hybridization in agaric fungi Karen W. Hughes1 to determine the outcome of hybridization events. Ronald H. Petersen Within Armillaria mellea and Amanita citrina f. Ecology and Evolutionary Biology, University of lavendula, we found evidence of interbreeding and Tennessee, Knoxville, Tennessee 37996-1100 recombination. Within G. dichrous and H. flavescens/ D. Jean Lodge chlorophana, hybrids were identified but there was Center for Forest Mycology Research, USDA-Forest no evidence for F2 or higher progeny in natural Service, Northern Research Station, Box 137, Luquillo, populations suggesting that the hybrid fruitbodies Puerto Rico 00773-1377 might be an evolutionary dead end and that the Sarah E. Bergemann genetically divergent Mendelian populations from which they were derived are, in fact, different species. Middle Tennessee State University, Department of Biology, PO Box 60, Murfreesboro Tennessee 37132 The association between ITS haplotype divergence of less than 5% (Armillaria mellea 5 2.6% excluding Kendra Baumgartner gaps; Amanita citrina f. lavendula 5 3.3%) with the USDA-Agricultural Research Service, Department of presence of putative recombinants and greater than Plant Pathology, University of California, Davis, California 95616 5% (Gymnopus dichrous 5 5.7%; Hygrocybe flavescens/ chlorophana 5 14.1%) with apparent failure of F1 2 Rodham E. Tulloss hybrids to produce F2 or higher progeny in popula- PO Box 57, Roosevelt, New Jersey 08555-0057 tions may suggest a correlation between genetic Edgar Lickey distance and reproductive isolation. -
Response of Ectomycorrhizal Fungal Fruiting to Nitrogen And
RESPONSE OF ECTOMYCORRHIZAL FUNGAL FRUITING TO NITROGEN AND PHOSPHORUS ADDITIONS IN BARTLETT EXPERIMENTAL FOREST, NEW HAMPSHIRE By Claudia N. Victoroff A thesis submitted in partial fulfillment of the requirements for the Master of Science Degree State University of New York College of Environmental Science and Forestry Syracuse, New York Department of Environmental and Forest Biology Approved by: Thomas R. Horton, Major Professor Theodore Dibble, Examining Committee Chairperson Melissa Fierke, Department Chairperson S. Scott Shannon, Dean, the Graduate School Acknowledgments Throughout the course of my master’s I have benefitted from the support of my lab mates, friends, and loved ones. I owe so much to my mentor Dr. Tom Horton. Tom has helped me to grow into a scientist. I entered ESF the summer after finishing my undergraduate and Tom’s guidance has helped me to develop away from insecurity and (closer) to self-directedness. The lab culture that Tom inspires is a cooperative and productive work environment and I am so thankful that I was able to be a part of it. The ESF community is unique. The curious minds of the undergraduates have inspired me, and the expertise of the faculty has challenged and motivated me. I have been supported through teaching assistantships by Tom (EFB 320 General Ecology Laboratory) and by Dr. Stewart Diemont (EFB 120 Global Environments Lecture). Tom and Stew have been excellent role models for me to adapt my own teaching style from. I am thankful for my graduate committee. Together my committee has directed my research and each member has benefitted my academic career significantly. -
Taxons BW Fin 2013
Liste des 1863 taxons en Brabant Wallon au 31/12/2013 (1298 basidios, 436 ascos, 108 myxos et 21 autres) [1757 taxons au 31/12/2012, donc 106 nouveaux taxons] Remarque : Le nombre derrière le nom du taxon correspond au nombre de récoltes. Ascomycètes Acanthophiobolus helicosporus : 1 Cheilymenia granulata : 2 Acrospermum compressum : 4 Cheilymenia oligotricha : 6 Albotricha acutipila : 2 Cheilymenia raripila : 1 Aleuria aurantia : 31 Cheilymenia rubra : 1 Aleuria bicucullata : 1 Cheilymenia theleboloides : 2 Aleuria cestrica : 1 Chlorociboria aeruginascens : 3 Allantoporthe decedens : 2 Chlorosplenium viridulum : 4 Amphiporthe leiphaemia : 1 Choiromyces meandriformis : 1 Anthostomella rubicola : 2 Ciboria amentacea : 9 Anthostomella tomicoides : 2 Ciboria batschiana : 8 Anthracobia humillima : 1 Ciboria caucus : 15 Anthracobia macrocystis : 3 Ciboria coryli : 2 Anthracobia maurilabra : 1 Ciboria rufofusca : 1 Anthracobia melaloma : 3 Cistella grevillei : 1 Anthracobia nitida : 1 Cladobotryum dendroides : 1 Apiognomonia errabunda : 1 Claussenomyces atrovirens : 1 Apiognomonia hystrix : 4 Claviceps microcephala : 1 Aporhytisma urticae : 1 Claviceps purpurea : 2 Arachnopeziza aurata : 1 Clavidisculum caricis : 1 Arachnopeziza aurelia : 1 Coleroa robertiani : 1 Arthrinium sporophleum : 1 Colletotrichum dematium : 1 Arthrobotrys oligospora : 3 Colletotrichum trichellum : 2 Ascobolus albidus : 16 Colpoma quercinum : 1 Ascobolus brassicae : 4 Coniochaeta ligniaria : 1 Ascobolus carbonarius : 5 Coprotus disculus : 1 Ascobolus crenulatus : 11