Test Report Comprehensive Hormone Insights™
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Steroid Profiling in Urine of Intact Glucuronidated and Sulfated
CORE Metadata, citation and similar papers at core.ac.uk Provided by Ghent University Academic Bibliography Journal of Chromatography A 1624 (2020) 461231 Contents lists available at ScienceDirect Journal of Chromatography A journal homepage: www.elsevier.com/locate/chroma Steroid profiling in urine of intact glucuronidated and sulfated steroids using liquid chromatography-mass spectrometry ∗ Laurie De Wilde , Kris Roels, Pieter Van Renterghem, Peter Van Eenoo, Koen Deventer 1 Doping Control Laboratory (DoCoLab), Ghent University (UGent), Department Diagnostic Sciences, Technologiepark 30B, B-9052 Zwijnaarde, Belgium a r t i c l e i n f o a b s t r a c t Article history: Detection of endogenous anabolic androgenic steroids (EAAS) misuse is a major challenge in doping con- Received 26 November 2019 trol analysis. Currently, a number of endogenous steroids, which constitute the steroid profile, are quanti- Revised 6 May 2020 fied using gas chromatography (GC). With this methodology, only the sum of the free and glucuronidated Accepted 10 May 2020 steroids is measured together. A dilute-and-shoot LC-MS method, which is compliant with the quality Available online 23 May 2020 requirements for measuring EAAS established by the World Anti-Doping Agency (WADA), was devel- Keywords: oped and validated containing glucuronidated and sulfated steroids in order to gain some extra infor- Doping mation and to expand the existing steroid profile. The developed method is, to the best of our knowl- Urine edge, the first method to combine both steroid glucuronides and sulfates, which is compliant with the Steroid profile quality standards of the technical document on EAAS, established by WADA. -
Estroquench™ Hormone Specific Formulation™
PRODUCT DATA DOUGLAS LABORATORIES® 08/2014 1 EstroQuench™ Hormone Specific Formulation™ DESCRIPTION EstroQuench™ is a Hormone Specific Formulation™ of ingredients that have documented anti-aromatase activity as well as androgenic adaptogens which support the function of endogenous aromatase inhibitors. Collectively these herbs promote minimal production and function of estrogens, while promoting testosterone function, including optimal sexual function in both genders. This formulation is designed to quench excessive production of estrogens and aberrant functions of them while supporting optimal function of androgens by maintaining the health of androgen producing glands.† Hormone Specific Formulation™ provided by Douglas Laboratories® and formulated by Dr. Joseph J Collins is created to support the optimal function of specific hormones through the use of hormone specific adaptogens, hormone specific agonists and hormone specific functional mimetics. This formulation may be used as part of a hormone health program with dietary and nutrient support. In addition, this formulation may be used by clinicians as an adjuvant to support optimal hormone health in patients who have been prescribed bioidentical hormone therapies. FUNCTIONS Aromatase (a cytochrome P450 enzyme {CYP19}) is the enzyme that controls the conversion of androgens to estrogens. More specifically, aromatase is the enzyme responsible for catalyzing the biosynthesis of androstenedione into estrone, and the biosynthesis of testosterone to estradiol. Estrogens include the broad range of aromatized hormones created form androgens. The specific attribute of estrogens that separate them from progestogens, androgens and corticoids is that estrogens are the only aromatized steroid hormones. Estradiol, the most potent endogenous estrogen, is biosynthesized by aromatization from androgens by aromatase (which is also called estrogen synthase). -
2018 Apigenin Male Infertility (2).Pdf
Original Article Thai Journal of Pharmaceutical Sciences Apigenin and baicalin, each alone or in low-dose combination, attenuated chloroquine induced male infertility in adult rats Amira Akilah, Mohamed Balaha*, Mohamed-Nabeih Abd-El Rahman, Sabiha Hedya Department of Pharmacology, Faculty of Medicine, Tanta University, Postal No. 31527, El-Gish Street, Tanta, Egypt Corresponding Author: Department of Pharmacology, ABSTRACT Faculty of Medicine, Tanta University, Postal No. 31527, Introduction: Male infertility is a worldwide health problem, which accounts for about 50% of all El-Gish Street, Tanta, Egypt. cases of infertility and considered as the most common single defined cause of infertility. Recently, Tel.: +201284451952. E-mail: apigenin and baicalin exhibited a powerful antioxidant and antiapoptotic activities. Consequently, in [email protected]. the present study, we evaluated the possible protective effect of apigenin and baicalin, either alone or Edu.Eg (M. Balaha). in low-dose combination, on a rat model of male infertility, regarding for its effects on the hormonal assay, testicular weight, sperm parameters, oxidative-stress state, apoptosis, and histopathological Received: Mar 06, 2018 changes. Material and methods: 12-week-old adult male Wister rats received 10 mg/kg/d Accepted: May 08, 2018 chloroquine orally for 30 days to induce male infertility. Either apigenin (30 or 15 mg/kg/d), baicalin Published: July 10, 2018 (100 or 50 mg/kg/d) or a combination of 15 mg/kg/d apigenin and 50 mg/kg/d baicalin received daily -
(Apigenin, Luteolin, Chrysin) and Their Importance for Health
Mellifera 2020, 20(1):16-27 MELLIFERA REVIEW ARTICLE Flavones (Apigenin, Luteolin, Chrysin) and Their Importance for Health Fatma Özsel ÖZCAN1*, Ozan ALDEMİR2, Bülent KARABULUT3 1Beykoz Üniversity, Faculty of Art and Design, Department of Gastronomy and Culinary Arts, İstanbul, Turkey 2Ege University Faculty of Medicine, Department of Biochemistry, İzmir, TURKEY 3Ege University Faculty of Medicine, Department of Oncology, İzmir, TURKEY * Corresponding author e-mail: [email protected] Received: 14th March, 2020; accepted: 4th May, 2020; published: 29th June, 2020 A B S T R A C T It has been shown in recent years that foods called functional foods may protect against certain types of cancer, cardiovascular diseases and cognitive dysfunctions. In the studies performed, the flavonoids (apigenin, chrysin, luteolin) which are subclass of flavonoids have been shown to have antioxidant, antiinflammatory, antiallergic, neuroprotective and cardioprotective effects and it is presented as the current treatment method in the treatment of some diseases. The structure function, nutritional resources and potential therapeutic properties of the flavones, which are also used as supplement in the compost, have been studied. The purpose of this review is to evaluate the therapeutic effects of flavones in certain diseases. The positive effect of 16 flavones on health can be proven in many experimental studies and can be proven in the long run. Keywords: Functional nutrients, flavones, apigenin, luteolin, chrysin Introduction Polyphenolic flavonoids are among the affected by factors such as plant species, wide variety of phytochemicals found in the environment, genetics, light, maturity, human diet. Current studies reveal that harvest [3]. Flavones, a subset of dietary flavonoids are inversely related to flavonoids, form glycosylation and contain many cancers and age-related diseases [1]. -
Normal Steroid Levels in Racehorses (Print) Page 1 of 2
The Horse | Normal Steroid Levels in Racehorses (print) Page 1 of 2 Normal Steroid Levels in Racehorses by: Christy West February 28 2010 Article # 15889 Steroid usage in racehorses has received a good deal of attention in the media, perhaps reaching a peak during the 2009 Triple Crown season when Big Brown won the Kentucky Derby and Preakness on the legally administered steroid stanozolol, then flopped in the Belmont without it. While no one could ever prove the steroid helped the horse win or that his loss was associated with being steroid-free, the situation added significant fuel to the fire of medication regulation in racehorses. One of the tough aspects of regulating substances that are naturally produced in the horse's body, such as many steroids, is that before you can decide how high a level of the substance constitutes an administered medication or abuse, you have to find out how much horses produce normally. At the 2009 American Association of Equine Practitioners (AAEP) Convention held Dec. 5-9 in Las Vegas, Nev., one presenter discussed a study that sought to answer that question for anabolic androgenic steroids (hormones that stimulate masculine physical characteristics) in young Thoroughbreds. Currently there are four anabolic androgenic steroids commonly used therapeutically in racehorses: Stanozolol, nandrolone, testosterone, and boldenone, said presenter Benjamin C. Moeller, BS, a graduate student at the K.L. Maddy Equine Analytical Chemistry Laboratory at the University of California, Davis. In collaboration with veterinarians at Rood & Riddle Equine Hospital and Hagyard Equine Medical Institute (both in Lexington, Ky.), and Craig Van Balen (private practitioner in Lexington), 142 un-medicated Thoroughbred colts and 62 fillies in training at Central Kentucky farms, from five to 24 months of age, were blood tested monthly for 13 months. -
Genetics of Androgen Disposition
From the Department of Laboratory Medicine Division of Clinical Pharmacology Karolinska Institutet, Stockholm, Sweden GENETICS OF ANDROGEN DISPOSITION - Implications for Doping Tests Jenny Jakobsson Schulze Stockholm 2007 All previously published papers were reproduced with permission from the publisher. Published by Karolinska Institutet. © Jenny Jakobsson Schulze, 2007 ISBN 978-91-7357-397-9 Printed by 2007 Gårdsvägen 4, 169 70 Solna One must sit down before truth without preconception, like a little child, and follow where the facts lead- or one will learn nothing. Thomas Huxley (1825-1895) To Joe and Dante ABSTRACT Anabolic androgenic steroids (AAS) are derivatives of testosterone. Doping with AAS is a severe challenge to the vision, moral and ethics in sports and has also become an increasing problem in society. Testosterone abuse is conventionally assessed by the urinary testosterone glucuronide/ epitestosterone glucuronide (T/E) ratio, levels above 4.0 being considered suspicious. However, there is a large inter-individual variation in testosterone glucuronide and epitestosterone glucuronide excretion, which challenges the accuracy of the test. There are reasons to believe that genetic variation is the single most important cause of variation in disposition of many androgenic compounds. Twin studies in men have demonstrated heritability estimates of 85% and 96% for production rates of testosterone and dihydrotestosterone, respectively. The primary aim of this thesis was to investigate the contribution of genetic components to inter-individual variation in androgen disposition. We found that a deletion polymorphism in the UGT2B17 gene was strongly associated with the urinary testosterone glucuronide levels. All individuals homozygous for the deletion had negligible amounts of urinary testosterone glucuronide. -
Development and Application of Methods for Extraction and LC/MS/MS Analysis of Sex Steroids and Conjugates from Fish Feces
Development and Application of Methods for Extraction and LC/MS/MS Analysis of Sex Steroids and Conjugates from Fish Feces By Lisa E. Peters A Thesis submitted to the Faculty of Graduate Studies of The University of Manitoba in partial fulfilment of the requirements of the degree of Doctor of Philosophy Department of Environment and Geography University of Manitoba Winnipeg Copyright © 2014 Lisa E. Peters 1 Acknowledgments I would like to start by thanking my supervisor, Dr. Gregg Tomy, for his support, patience and resources during my Ph.D. program. He took a chance when I tried to convince him that my biology background would be a great addition to his chemistry lab. My husband, Vince Palace, and I also appreciated his support and genuine happiness for us when I announced we were expecting a baby in the middle of my studies. A sincere thanks also goes to my co-supervisor, Dr. Mark Hanson, for his support on so many levels (I wouldn’t even know where to start), and for doing his best to keep me on track, which was no small task. I would also like to thank my other thesis committee members, Drs. Gary Anderson and Feiyue Wang, for their encouragement and insightful comments. Gary gave a lot of extra time to review various thesis chapters and data, and to lend his expertise during the fish surgeries. I also had the pleasure of taking his Endocrinology course, which was probably the most informative and enjoyable class of my entire university student career. I would like to thank the technical staff and students from DFO, Suzanne Mittermuller, Kerry Wautier, Alea Goodmanson, Danielle Godard, Lisa Friedrich and Kirstin Dangerfield, and my lab mates Bruno Rosenberg, Kerri Pleskach, Colin Darling, Bonnie Gemmill and Lianna Bestvater for their technical input and help during my experiments. -
Inhibitory Effect of Acacetin, Apigenin, Chrysin and Pinocembrin on Human Cytochrome P450 3A4
ORIGINAL SCIENTIFIC PAPER Croat. Chem. Acta 2020, 93(1), 33–39 Published online: August 03, 2020 DOI: 10.5562/cca3652 Inhibitory Effect of Acacetin, Apigenin, Chrysin and Pinocembrin on Human Cytochrome P450 3A4 Martin Kondža,1 Hrvoje Rimac,2,3 Željan Maleš,4 Petra Turčić,5 Ivan Ćavar,6 Mirza Bojić2,* 1 University of Mostar, Faculty of Pharmacy, Matice hrvatske bb, 88000 Mostar, Bosnia and Herzegovina 2 University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, A. Kovačića 1, 10000 Zagreb, Croatia 3 South Ural State University, Higher Medical and Biological School, Laboratory of Computational Modeling of Drugs, 454000 Chelyabinsk, Russian Federation 4 University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, 10000 Zagreb, Croatia 5 University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmacology, Domagojeva 2, 10000 Zagreb, Croatia 6 University of Mostar, Faculty of Medicine, Kralja Petra Krešimira IV bb, 88000 Mostar, Bosnia and Herzegovina * Corresponding author’s e-mail address: [email protected] RECEIVED: June 26, 2020 REVISED: July 28, 2020 ACCEPTED: July 30, 2020 Abstract: Cytochrome P450 3A4 is the most significant enzyme in metabolism of medications. Flavonoids are common secondary plant metabolites found in fruits and vegetables. Some flavonoids can interact with other drugs by inhibiting cytochrome P450 enzymes. Thus, the objective of this study was to determine inhibition kinetics of cytochrome P450 3A4 by flavonoids: acacetin, apigenin, chrysin and pinocembrin. For this purpose, testosterone was used as marker substrate, and generation of the 6β-hydroxy metabolite was monitored by high performance liquid chromatography coupled with diode array detector. -
Cphi & P-MEC China Exhibition List展商名单version版本20180116
CPhI & P-MEC China Exhibition List展商名单 Version版本 20180116 Booth/ Company Name/公司中英文名 Product/产品 展位号 Carbosynth Ltd E1A01 Toronto Research Chemicals Inc E1A08 SiliCycle Inc. E1A10 SA TOURNAIRE E1A11 Indena SpA E1A17 Trifarma E1A21 LLC Velpharma E1A25 Anuh Pharma E1A31 Chemclone Industries E1A51 Hetero Labs Limited E1B09 Concord Biotech Limited E1B10 ScinoPharm Taiwan Ltd E1B11 Dongkook Pharmaceutical Co., Ltd. E1B19 Shenzhen Salubris Pharmaceuticals Co., Ltd E1B22 GfM mbH E1B25 Leawell International Ltd E1B28 DCS Pharma AG E1B31 Agno Pharma E1B32 Newchem Spa E1B35 APEX HEALTHCARE LIMITED E1B51 AMRI E1C21 Aarti Drugs Limited E1C25 Espee Group Innovators E1C31 Ruland Chemical Co., Ltd. E1C32 Merck Chemicals (Shanghai) Co., Ltd. E1C51 Mediking Pharmaceutical Group Ltd E1C57 珠海联邦制药股份有限公司/The United E1D01 Laboratories International Holdings Ltd. FMC Corporation E1D02 Kingchem (Liaoning) Chemical Co., Ltd E1D10 Doosan Corporation E1D22 Sunasia Co., Ltd. E1D25 Bolon Pharmachem Co., Ltd. E1D26 Savior Lifetec Corporation E1D27 Alchem International Pvt Ltd E1D31 Polish Investment and Trade Agency E1D57 Fischer Chemicals AG E1E01 NGL Fine Chem Limited E1E24 常州艾柯轧辊有限公司/ECCO Roller E1E25 Linnea SA E1E26 Everlight Chemical Industrial Corporation E1E27 HARMAN FINOCHEM E1E28 Zhechem Co Ltd E1F01 Midas Pharma GmbH Shanghai Representativ E1F03 Supriya Lifescience Ltd E1F10 KOA Shoji Co Ltd E1F22 NOF Corporation E1F24 上海贺利氏工业技术材料有限公司/Heraeus E1F26 Materials Technology Shanghai Ltd. Novacyl Asia Pacific Ltd E1F28 PharmSol Europe Limited E1F32 Bachem AG E1F35 Louston International Inc. E1F51 High Science Co Ltd E1F55 Chemsphere Technology Inc. E1F57a PharmaCore Biotech Co., Ltd. E1F57b Rockwood Lithium GmbH E1G51 Sarv Bio Labs Pvt Ltd E1G57 抗病毒类、抗肿瘤类、抗感染类和甾体类中间体、原料药和药物制剂及医药合约研发和加工服务 上海创诺医药集团有限公司/Shanghai Desano APIs and Finished products of ARV, Oncology, Anti-infection and Hormone drugs and E1H01 Pharmaceuticals Co., Ltd. -
Characterization of the CYP3A4 Enzyme Inhibition Potential of Selected Flavonoids
Article Characterization of the CYP3A4 Enzyme Inhibition Potential of Selected Flavonoids Martin Kondža 1 , Mirza Boji´c 2,* , Ivona Tomi´c 1, Željan Maleš 2 , Valentina Rezi´c 3 and Ivan Cavar´ 4 1 Faculty of Pharmacy, University of Mostar, Matice Hrvatske bb, 88000 Mostar, Bosnia and Herzegovina; [email protected] (M.K.); [email protected] (I.T.) 2 University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovaˇci´ca1, 10000 Zagreb, Croatia; [email protected] 3 Farmavita d.o.o., Igmanska 5A, 71000 Sarajevo, Bosnia and Herzegovina; [email protected] 4 Faculty of Medicine, University of Mostar, Zrinskog Frankopana 34, 88000 Mostar, Bosnia and Herzegovina; [email protected] * Correspondence: [email protected]; Tel.: +385-1-4818-304 Abstract: Acacetin, apigenin, chrysin, and pinocembrin are flavonoid aglycones found in foods such as parsley, honey, celery, and chamomile tea. Flavonoids can act as substrates and inhibitors of the CYP3A4 enzyme, a heme containing enzyme responsible for the metabolism of one third of drugs on the market. The aim of this study was to investigate the inhibitory effect of selected flavonoids on the CYP3A4 enzyme, the kinetics of inhibition, the possible covalent binding of the inhibitor to the enzyme, and whether flavonoids can act as pseudo-irreversible inhibitors. For the determination of inhibition kinetics, nifedipine oxidation was used as a marker reaction. A hemochromopyridine test was used to assess the possible covalent binding to the heme, and incubation with dialysis was used in order to assess the reversibility of the inhibition. -
Hormone Testing Summary All Units Are Given in Ng/Mg Creatinine
Accession # 00212403 Sample Female Report 123 A Street Sometown, CA 90266 Collection Times: Ordering physician: DOB:1976-01-01 2015-08-16 05:00PM 2015-08-16 10:00PM Precision Analytical Gender: Female 2015-08-17 06:00AM 2015-08-17 08:00AM Hormone Testing Summary All units are given in ng/mg creatinine Sex Hormones See Pages 2 and 3 for a thorough breakdown of sex hormone metabolites opausa n l R e a m n e r g P e your 27.0 62.0 6.0 4.0 14.0 low limit high limit 80.5 17.9 12.8 result Postmenopausal 20.0 range 6.0-17.0 0.3-2.0 Total Estrogen Progesterone Testosterone How to read the graphical (Sum of 8 Estrogen Metabolites) (Serum Equivalent, ng/mL) representation of results Progesterone Serum Equivalent is a calculated value based on urine pregnanediol. This value may not accurately reflect serum when progesterone is taken by mouth. Adrenal Hormones See pages 4 and 5 for a more complete breakdown of adrenal hormones Total DHEA Production 100 High Range Limit 400.0 2500.0 ) 2218.0 g Age Range m Daily Free Cortisol Pattern / g 20-40 800-2500 n 80 ( 40-60 530-1550 Total DHEA Production l >60 400-1350 (DHEAS + Etiocholanolone + Androsterone) o s 60 i t r o C 40 2240.0 4300.0 Patient Values Low Range Limit 80.0 48.0 180.0 5033.0 20 24hr Free Cortisol cortisol Metabolized Cortisol (THF+THE) 0 (A+B+C+D) metabolism (Total Cortisol Production) Waking (A) Morning (B) Afternoon (C) Night (D) Free cortisol best reflects tissue levels. -
Metabolism of Testoterone and Related Steroids in Metastatic Interstitial Cell Carcinoma of the Testis
Metabolism of testoterone and related steroids in metastatic interstitial cell carcinoma of the testis. M B Lipsett, … , C W Bardin, L M Fishman J Clin Invest. 1966;45(11):1700-1709. https://doi.org/10.1172/JCI105476. Research Article Find the latest version: https://jci.me/105476/pdf Journal of Clinical Investigation Vol. 45, No. 11, 1966 Metabolism of Testosterone and Related Steroids in Metastatic Interstitial Cell Carcinoma of the Testis * M. B. LIPSETT,t G. A. SARFATY, H. WILSON, C. WAYNE BARDIN, AND L. M. FISHMAN (From the Endocrinology Branch, National Cancer Institute, Bethesda, Md.) Interstitial cell carcinoma of the testis is a singu- production rate has been shown to be a conse- larly rare steroid-producing cancer. Of the seven quence of metabolism of dehydroepiandrosterone reported cases (1-7), urinary 17-ketosteroid (17- sulfate. KS) excretion was high in the four cases in which it was measured. Abelson, Bulaschenko, Trom- Methods mer, and Valdes-Dapena (7) fractionated the uri- Routine methods were used to analyze the following: nary 17-ketosteriods and corticoids in one recently urinary 17-KS (8), urinary 17-hydroxycorticoids (9), reported case. There is, however, no comprehen- plasma Silber-Porter chromogens (10), and plasma tes- tosterone (11). sive study of either the production of androgens Gas-liquid chromatography. We carried out gas-liquid or related steroids by this tumor. We have had chromatography (GLC) in a Glowell Chromolab gas the opportunity to study a patient with metastatic chromatograph utilizing a 'Sr ionization detector oper- interstitial cell carcinoma, and we have examined ating at 1,050 v.