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Zoonotic Diseases July 02-04, 2018 | Vienna, Austria conferenceseries.com JOINT EVENT 10th International Virology Summit & 4th International Conference on Influenza & Zoonotic Diseases July 02-04, 2018 | Vienna, Austria Scientific Tracks & Abstracts Day 1 Euro Virology 2018 & Influenza 2018 Page 47 Session-1 Day 1 July 02, 2018 Human Virology and Hepatitis|HIV, AIDS and other Emerging Viruses |Molecular Virology Medical Virology |Plant Virology | Current Focus in Virology Research | Viral Oncology Current and Novel Approach for Influenza vaccines | Influenza Vaccines and Vaccination Pathogenesis of Influenza Virus | Influenza Viruses -Advance in Detection & Differentiation Session Chair Session Co-Chair Joseph C. Glorioso Alfredo Berzal-Herranz University of Pittsburgh, USA Institute of Parasitology and Biomedicine, Spain Session Introduction Title: Role of Herpesviruses dUTPases in the immune dysregulation associated with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome and Gulf war illness Maria Eugenia Ariza, The Ohio State University, USA Title: Generation of monoclonal antibodies against foot-and-mouth disease virus SAT 2 and development of lateral flow strip test for virus detection Ming Yang, Canadian Food Inspection Agency, Canada Title: Proteins candidates for complementary diagnosis of goats naturally infected by Lentivirus Angela Maria Xavier Eloy, Brazilian Agricultural Research Corporation, Brazil Title: Protein binders mimicking surface glycoprotein epitopes recognized by broadly neutralizing antibodies as a new platform for identification of peptide-prints as tools for development of more protective vaccines Petr Maly, Czech Academy of Sciences, Czech Republic Title: Long-read sequencing uncovers a hidden complexity of the herpesvirus transcriptome Zsolt Boldogkoi, University of Szeged, Hungary Title: AIDS process from the perspective of evolution Zajac Vladimir, Cancer Research Institute BMC SAS, Slovakia Title: A.I.R vaccines – A synthetic self-amplifying RNA-based vaccine against Influenza Annette B Vogel, Biopharmaceutical New Technologies (BioNTech) Corporation, Germany Title: Velvet bean severe mosaic begomovirus DNA-A encoded RNA silencing suppressor proteins Narayan Rishi, Amity University, India Title: Pathogenesis of the adapted a(h1n1)pdm09 influenza viruses in different organs of infected mice Prokopyeva Elena, Novosibirsk State University, Russia Title: Comprehensive analysis of Vaccinia virus transcriptome by third-generation sequencing Dora Tombacz, University of Szeged, Hungary Title: The protective efficacy of zika polyclonal antibodies against lethal zika virus infection in ifnar1-/- mouse model Shantha Kodihalli, Emergent BioSolutions Canada, Canada Title: Multiplex detection of Aspergillus fumigatus mycoviruses Selin Ozkan-Kotiloglu, Ahi Evran University, Turkey Title: Metagenomic Analysis of Viral Diversity in Respiratory samples from patients with Respiratory Tract Infections in Kuwait Nada Madi, Kuwait University, Kuwait Title: Persistent TGEV infection enhances ETEC K88 adhesion by promoting epithelialmesenchymal transition in intestinal epithelial cells Qian Yang, Nanjing Agricultural University, China Title: A novel pathway of virus dissemination within the host: spread of porcine YuchenEuro Li, Nanjing Virology Agricultural University, 2018 China & Influenza 2018 Page 48 Maria Eugenia Ariza et al., Virol Mycol 2018, Volume 7 conferenceseries.com DOI: 10.4172/2161-0517-C2-026 JOINT EVENT 10th International Virology Summit & 4th International Conference on Influenza & Zoonotic Diseases July 02-04, 2018 | Vienna, Austria Role of Herpesviruses dUTPases in the immune dysregulation associated with myalgic encephalomyelitis/chronic fatigue syndrome and Gulf war illness Maria Eugenia Ariza1,2, Marshall V Williams1,2, Brandon Cox2 and Conner Nealer2 1Wexner Medical Center - The Ohio State University, USA 2Institute for Behavioral Medicine Research, USA yalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and Gulf war illness (GWI) are debilitating diseases Mpresenting with complex immune, endocrine and neurological symptoms. Annual health care costs are estimated at $24 billion. Diagnosis is based on exclusion and there are currently no validated tests for definitive diagnosis of either syndrome. While there is accumulating evidence supporting the premise that some herpesviruses may act as possible triggers in ME/CFS, the mechanism by which they contribute to the pathogenesis of ME/CFS remains unclear. Our studies are the first to demonstrate that the deoxyuridine triphosphate nucleotidohydrolase (dUTPase) encoded by the human herpesviruses represents a new class of pathogen-associated molecular pattern (PAMP) proteins, which alter immune and neurocognitive functions. In this study, we demonstrate that ME/CFS and GWI patients’ sera exhibit reactivation of multiple herpesviruses, differential antibody expression patterns to the herpesviruses-encoded dUTPase early protein as well as increased autoantibodies to the human nuclear dUTPase. A significant increase in IL-21 levels was also observed in a cohort of ME/CFS patients. Interestingly, IL-21 is produced at high levels by CD4+ follicular helper T cell (TFH) and regulates germinal center (GC) B cell survival and plasma- cell differentiation. Further in vitro studies in primary human cells demonstrated that the EBV-dUTPase induced activin A secretion by dendritic cells, which lead to the increased formation of CD4+ follicular helper T cells (TFH) and subsequent production of IL-21 and CXCL13 by TFH. Our data suggest a role for the herpesviruses dUTPase proteins in the immune dysregulation and pathophysiology observed in these patients possibly by altering the GC reaction and antibody responses as well as inducing the production of pleiotropic cytokines. Thus, screening for the presence of anti-herpesvirus dUTPase antibodies in these patients may serve as useful diagnostic biomarkers for the selection of appropriate treatments. Recent Publications 1. Saito T, Miyagawa K, Chen S Y, Tamosiuniene R, Wang L, Sharpe O, Samayoa E, Harada D, Grow E, Moonen J R, Cao A, Chen P I, Hennigs J K, Gu M, Li C G, Leib R D, Li D, Adams C M, del Rosario, P A, Bill M, Haddad F, Montoya J G, Robinson W H, Fantl W J, Nolan G, Zamanian R T, Nicolls M R, Chiu C Y, Ariza M E and Rabinovitch M (2017) Upregulation of HERV-K is linked to immunity and inflammation in pulmonary arterial hypertension. Circulation 136(20):1920-1935. 2. Halpin P, Williams M, Klimas N G, Fletcher M A, Barnes Z and Ariza M E (2017) Differential antibody patterns to the herpesviruses-encoded dUTPases in myalgic encephalomyelitis/chronic fatigue syndrome and Gulf war illness. Journal of Medical Virology 89:1636–1645. 3. Williams M, Cox B and Ariza M E (2017) Herpesviruses dUTPases: A new family of pathogen-associated molecular pattern (PAMP) proteins with implications for human disease. Pathogens DOI: 10.3390/pathogens6010002. 4. Young N A, Williams M V, Jarjour W N, Bruss M S, Bolon B, Parikh S, Satoskar A and Ariza M E (2016) Epstein-Barr virus (EBV) encoded dUTPase exacerbates the immune pathology of lupus nephritis in vivo. International Journal of Immunology and Immunotherapy 3(2):023. 5. Aubrecht T G, Weil Z M, Salloum B A, Ariza M E, Williams M, Reader B, Glaser R, Sheridan J and Nelson R J (2015) Chronic physical stress does not interact with Epstein-Barr virus (EBV) encoded dUTPase to alter the sickness response. J Behavioral Brain Science 5:513-523. Virology & Mycology Volume 7 ISSN: 2161-0517 Euro Virology 2018 & Influenza 2018 July 02-04, 2018 Page 49 conferenceseries.com JOINT EVENT 10th International Virology Summit & 4th International Conference on Influenza & Zoonotic Diseases July 02-04, 2018 | Vienna, Austria Biography Maria Eugenia Ariza received her PhD degree in Medical Microbiology and Immunology and is currently a Research Assistant Professor in the Department of Cancer Biology and Genetics, College of Medicine, at The Ohio State University. She has a long-standing passion for understanding how viruses contribute to the pathophysiology of human diseases, specifically the role that the human herpesviruses and human endogenous retroviruses (HERVs) have in the development of autoimmune diseases and cancer. Her studies were the first to demonstrate that the dUTPases from the human herpesviruses and HERV-K represent a new class of pathogen-associated molecular pattern (PAMP) proteins, which contribute to the immune pathology associated with several immune-mediated diseases, including chronic fatigue syndrome (CFS), SLE, psoriasis and pulmonary arterial hypertension. Her studies have identified this family of viral dUTPases as potential disease biomarkers and provide novel molecular targets for the development of alternative therapeutic agents/interventions for CFS, SLE and psoriasis. [email protected] Notes: Virology & Mycology Volume 7 ISSN: 2161-0517 Euro Virology 2018 & Influenza 2018 July 02-04, 2018 Page 50 Ming Yang et al., Virol Mycol 2018, Volume 7 conferenceseries.com DOI: 10.4172/2161-0517-C2-026 JOINT EVENT 10th International Virology Summit & 4th International Conference on Influenza & Zoonotic Diseases July 02-04, 2018 | Vienna, Austria Generation of monoclonal antibodies against foot-and-mouth disease virus SAT 2 and development of lateral flow strip test for virus detection Ming Yang1, Kaye Quizon1, Boitumelo Mudabuka2 and Charles Nfon1 1National Centre for Foreign Animal Disease, Canada 2Botswana Institute for Technology Research and Innovation, Botswana oot-and-mouth disease (FMD) remains one of
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