DOCSLIB.ORG

R2A/MAC Biopaddles™ Colony Identification App Code 5540

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

LaMotte BioPaddles™ TECH DOCUMENT Call: 800-344-3100 Email: [email protected] COMING SOON! R2A/MAC BioPaddles™ Colony Identification App Code 5540 R2A Agar (R2A) MacConkey Agar (MAC) USE: Enumeration and cultivation of bacteria from potable water; total coliform testing (TCC). Coliform Testing / Recovery of Stressed Coliforms Side 1: R2A Agar (R2A) (colorless) (Side 1 is marked with an indented laser line) Side 2: MacConkey Agar (MAC) (pink) APPLICATION For bacterial plate counts of treated potable water as well as oligotrophic heterotrophs. In total coliform testing (TCC), the coliform organisms tested for include: total coliform, fecal coliform, and E. coli (Escherichia coli). Detection of fecal coliforms (a subset of total coliforms) or Escherichia coli (a subset of fecal coliforms) can indicate the potential presence of waterborne pathogens associated with fecal contamination.1 R2A Agar is recommended in Standard Methods for the Examination of Water and Wastewater for pour plate, spread plate and membrane filter methods for heterotrophic counts. PADDLE AGARS R2A Agar (R2A) – Developed by Reasoner and Geldreich2 for bacterial plate counts of treated potable water as well as oligotrophic (thrive in low-nutrient conditions) heterotrophs. R2A Agar, in combination with a lower incubation temperature and longer incubation time, stimulates the growth of stressed and chlorine-tolerant bacteria. Enzymatic digest of casein and animal tissue provides nitrogen, carbon and minerals in R2A Agar. Yeast extract is a source of vitamins and trace elements. Dextrose serves as a carbon source. Soluble starch aids in the recovery of injured organisms by absorbing toxic metabolic by-products. Dipotassium Phosphate is used to balance the pH, and magnesium sulfate heptahydrate is a source of divalent cations and sulfate. Sodium pyruvate increases the recovery of stressed cells. Agar and a proprietary polymer are the solidifying agents. NOTE: Fast-growing bacteria may produce smaller size colonies on R2A Agar than on nutritionally rich media. R2A Agar is a low nutrient medium intended for culturing compromised microorganisms. 1 United States Pharmacopeial Convention. 2007. The United States pharmacopeia, 31st ed., Amended Chapters 61, 62, 111. The United States Pharmacopeial Convention, Rockville, MD. 2 Reasoner, D. J., and E. E. Geldreich. 1979. A new medium for the enumeration and subculture of bacteria from potable water. Abstracts of the Annual Meeting of the American Society for Microbiology 79th Meeting, Paper No. N7. For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory. ©LaMotte BioPaddles. All rights reserved. LaMotte_BioPaddles_R2A_MAC Page | 1 LaMotte BioPaddles™ TECH DOCUMENT Call: 800-344-3100 Email: [email protected] MacConkey Agar (MAC) – Both selective and differential; used to differentiate between Gram negative bacteria while inhibiting the growth of most Gram positive bacteria. The medium also differentiates between lactose-fermenting coliforms Lac (+) and lactose non-fermenters Lac (-), which include potential pathogens. Addition to the nutrient agar base of bile salts and crystal violet will inhibit the growth of most Gram-positive bacteria, making MacConkey agar selective. Lactose, a fermentable carbohydrate, and neutral red, a pH indicator, are added to differentiate the lactose positive coliforms from the potentially pathogenic lactose non-fermenters. When lactose is fermented, acid products lower the pH below 6.8, with the resulting colonial growth turning pinkish-red. If an organism is unable to ferment lactose, the colonies will be colorless. Bile salts mixture and crystal violet are the selective agents, inhibiting Gram positive cocci and allowing Gram-negative organisms to grow. Sodium Chloride maintains the osmotic environment. Agar and a proprietary polymer are the solidifying agents. CULTURE CONTROLS 10-300 inoculum (CFU) R2A Agar MacConkey Agar Bacillus subtilis GROWTH INHIBITED Enterococcus faecalis GROWTH INHIBITED Escherichia coli GROWTH GROWTH Pseudomonas aeruginosa GROWTH GROWTH Staphylococcus aureus GROWTH GROWTH STORAGE/EXPIRATION Store tightly sealed BioPaddles™ in a cool, dry location (less than 68°F/20°C). Avoid temperature changes. BioPaddles™ may be refrigerated, but it is not necessary. Do not freeze. If freezing occurs, thaw (3-6 hours) under refrigeration temperatures (40°F; 4.4°C). Freezing can promote excess water loss and variation in media surface due to crystal formation. The average shelf-life is one year. Refer to expiration date (See: BBE stamped on vial). Discard if paddle agar appears oxidized (darkens from expected color). The expiration date applies to the medium in an intact container when stored as directed. SAMPLING Liquids: Twist to remove paddle from vial. Fill vial to 40 mL fill line with the liquid to be sampled. The 40 mL volume can be used to calculate Total Viable Count (TVC) and/or Total Colony Count (TCC). Replace paddle. Allow a contact time of 15 seconds. Remove the paddle. Empty the vial. Replace the paddle in the vial. Surfaces: Twist to remove paddle from vial. Allow the paddle surface (10 cm2) to come into physical contact with the test surface. Recovery rate is about 50%. To insure an accurate recovery, gently sweep (or touch) the paddle to cover a 20 cm2 area. Replace paddle in vial. For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory. ©LaMotte BioPaddles. All rights reserved. LaMotte_BioPaddles_R2A_MAC Page | 2 LaMotte BioPaddles™ TECH DOCUMENT Call: 800-344-3100 Email: [email protected] INCUBATION Incubate at 37°C for 24 hours, continue at 25°C for 3-5 days. NOTE: Fast-growing bacteria may produce smaller size colonies on R2A Agar than on nutritionally rich media. R2A Agar is a low nutrient medium intended for culturing compromised microorganisms. COLONY MEASURING Each BioPaddles™ paddle has molded media attachment points that are 4mm in length (point-to-point). This feature provides a useful guidepost to estimating nearby colony size. IDENTIFICATION ORGANISM R2A MAC ORGANISM PHYSIOLOGY GROWTH COLONY IMAGE GROWTH COLONY IMAGE ◆ Precision Test Strip Available Aspergillus niger • Catalase (+) • Granular • Ascomycete • Jet black conidia w/ INHIBITED --- --- +++ yellow/gray hyphae • 3-5++cm Bacillus spp. • Lactose (-) • Translucent to • Indole (-) ◆ dull, off-white; • Oxidase (-) ◆ opaque • PARTIAL - • Catalase (+) ◆ Smooth to rough COMPLETE ◆ +++ • irregular / --- • Urease () INHIBITION • Gram (+) Rod dendroid margins to spreading • 2-4mm For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory. ©LaMotte BioPaddles. All rights reserved. LaMotte_BioPaddles_R2A_MAC Page | 3 LaMotte BioPaddles™ TECH DOCUMENT Call: 800-344-3100 Email: [email protected] ORGANISM R2A MAC ORGANISM PHYSIOLOGY GROWTH COLONY IMAGE GROWTH COLONY IMAGE ◆ Precision Test Strip Available Candida albicans • Catalase (+) • White / Opaque • Ascomycete • CVEG / PARTIAL - spreading COMPLETE --- --- +++ • 4-6mm INHIBITION E. coli • Lactose (+) • Translucent +++ • Pink / Red • Indole (+) ◆ • CVEG • CVEG • Oxidase (-) ◆ • 0.5 - 1.0mm • 2-4mm ++ • Catalase (+) ◆ • Urease (-) ◆ • Gram (-) Rod) Enterobacter • Lactose (+) • White • Pink aerogenes • Indole (-) ◆ • Thick, round, • Thick, round, • Oxidase (-) ◆ smooth, raised smooth, raised IMAGE • Catalase (+) ◆ to low-convex; to low-convex; PENDING • Urease (-) ◆ +++ Spreading +++ Spreading • Gram (-) Rod • 2-3mm • 2-3mm Enterococcus spp. • Lactose (-) • Clear • Indole (-) ◆ • CVEG IMAGE • Oxidase (-) ◆ • 1-2mm PENDING PARTIAL - COMPLETE • Catalase (-) ◆ +++ --- --- INHIBITION • Urease (-) ◆ • Gram (+) Sphere Klebsiella spp. • Lactose (+) • White +++ • Colorless / • Indole (-) ◆ • CVEG Light Pink • Oxidase (-) ◆ • 1-2mm • Spreading +++ IMAGE • Catalase (+) ◆ PENDING • 4-5mm • Urease (+) ◆ • Gram (-) Rod Proteus spp. • Lactose (-) • White +++ Colorless; • SEE: INDOLE ◆ • CVEG Spreading / • Oxidase (-) ◆ • 1-2mm IMAGE swarming PENDING • Catalase (+) ◆ +++ • 2-3mm • Urease (+) ◆ • Gram (-) Rod For in vitro diagnostic use only. This product should be used only by adequately trained personnel with knowledge of microbiological techniques in the laboratory. ©LaMotte BioPaddles. All rights reserved. LaMotte_BioPaddles_R2A_MAC Page | 4 LaMotte BioPaddles™ TECH DOCUMENT Call: 800-344-3100 Email: [email protected] ORGANISM R2A MAC ORGANISM PHYSIOLOGY GROWTH COLONY IMAGE GROWTH COLONY IMAGE ◆ Precision Test Strip Available Pseudomomas • Lactose (-) • Greenish-white • Cream / off- aeruginosa • Indole (-) ◆ and translucent white • Oxidase (+) ◆ • 2-4mm • Irregular • Catalase (+) ◆ /Spreading IMAGE IMAGE ◆ +++ +++ • Diffusible • Urease (-) PENDING PENDING • Gram (-) Rod green-blue pigment • Fluoresces blue under long-wave • CVEG UV light (400-nm) • 2-4mm Pseudomomas • Lactose (-) • Clear to amber • Clear or fluorescens • Indole (-) ◆ • CVEG pinkish • Oxidase (+) ◆ • 2-4mm • Irregular • Catalase (+) ◆ edges • Urease (-) ◆ • Clear with • Gram (-) Rod +++ +++ dark, pinkish • Fluoresces blue- centers green under (translucent long-wave UV light edges) (400-nm) • 2-4mm +++ Salmonella • Lactose (-) • White / • Clear, typhimurium • Indole (-) ◆ Translucent transparent or • Oxidase (-) ◆ • CVEG IMAGE amber IMAGE PENDING PENDING • Catalase (+) ◆ +++ • 2-4mm • Circular,
Recommended publications
  • Microbiology Media - Ready to Use, Prepared Plates

    Microbiology Media - Ready to Use, Prepared Plates

    Microbiology Media - Ready to use, prepared plates Ready poured plates, general purpose media Ready poured plate, general anaerobe agar 65 65 Columbia blood agar with neomycin. Chromogenic UTI medium Catalogue No Plate diameter, mm Pack qty Price Catalogue No Pack qty Price PO794A 90 10 12.55 PO219A 10 7.54 CLED medium Catalogue No Plate diameter, mm Pack qty Price Ready poured plate, Lactobacilli PO120A 90 10 5.75 64 CLED square plate Catalogue No Plate dimensions, mm Pack qty Price MRS Agar. OXPO0299L 120 x 120 10 16.92 CLED medium with andrades Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO231A 90 10 7.30 PO121A 90 10 5.75 Columbia agar base Ready poured plates, Legionnella media Catalogue No Plate diameter, mm Pack qty Price 65 OXPO0537A 90 10 5.45 MacConkey agar with salt Legionella growth medium, BCYE Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO149A 90 10 5.95 PO5072A 90 10 14.72 MacConkey agar without salt Legionella selective medium, BMPA Catalogue No Plate diameter, mm Pack qty Price Catalogue No Plate diameter, mm Pack qty Price PO148A 90 10 5.58 PO0324A 90 10 20.31 MacConkey agar No. 3 Catalogue No Plate diameter, mm Pack qty Price PO495A 90 10 5.90 Malt extract agar Catalogue No Plate diameter, mm Pack qty Price PO182A 90 10 6.38 MRSA agar Catalogue No Plate diameter, mm Pack qty Price OXPO1162A 90 10 15.49 Nutrient agar Catalogue No Plate diameter, mm Pack qty Price PO155A 90 10 5.75 Plate count agar Catalogue No Plate diameter, mm Pack qty Price PO158A 90 10 6.36 R2A agar Catalogue No Plate diameter, mm Pack qty Price PO659A 90 10 6.50 Sabouraud dextrose agar Catalogue No Plate diameter, mm Pack qty Price OXPO0160A 90 10 5.54 Sorbitol MacConkey agar Catalogue No Plate diameter, mm Pack qty Price PO232A 90 10 5.84 Tryptone soya agar Catalogue No Plate diameter, mm Pack qty Price PO163A 90 10 5.49 OXPO0193-C 55 10 10.91 Yeast extract agar Catalogue No Plate diameter, mm Pack qty Price PO441A 90 10 5.08 55.
  • Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment

    Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment

    antibiotics Article Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment Alicia Sorgen 1, James Johnson 2, Kevin Lambirth 2, Sandra M. Clinton 3 , Molly Redmond 1 , Anthony Fodor 2 and Cynthia Gibas 2,* 1 Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (A.S.); [email protected] (M.R.) 2 Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (J.J.); [email protected] (K.L.); [email protected] (A.F.) 3 Department of Geography & Earth Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-704-687-8378 Abstract: Bacterial resistance to antibiotics is a growing global concern, threatening human and environmental health, particularly among urban populations. Wastewater treatment plants (WWTPs) are thought to be “hotspots” for antibiotic resistance dissemination. The conditions of WWTPs, in conjunction with the persistence of commonly used antibiotics, may favor the selection and transfer of resistance genes among bacterial populations. WWTPs provide an important ecological niche to examine the spread of antibiotic resistance. We used heterotrophic plate count methods to identify Citation: Sorgen, A.; Johnson, J.; phenotypically resistant cultivable portions of these bacterial communities and characterized the Lambirth, K.; Clinton,
  • Food Microbiology

    Food Microbiology

    Food Microbiology Food Water Dairy Beverage Online Ordering Available Food, Water, Dairy, & Beverage Microbiology Table of Contents 1 Environmental Monitoring Contact Plates 3 Petri Plates 3 Culture Media for Air Sampling 4 Environmental Sampling Boot Swabs 6 Environmental Testing Swabs 8 Surface Sanitizers 8 Hand Sanitation 9 Sample Preparation - Dilution Vials 10 Compact Dry™ 12 HardyCHROM™ Chromogenic Culture Media 15 Prepared Media 24 Agar Plates for Membrane Filtration 26 CRITERION™ Dehydrated Culture Media 28 Pathogen Detection Environmental With Monitoring Contact Plates Baird Parker Agar Friction Lid For the selective isolation and enumeration of coagulase-positive staphylococci (Staphylococcus aureus) on environmental surfaces. HardyCHROM™ ECC 15x60mm contact plate, A chromogenic medium for the detection, 10/pk ................................................................................ 89407-364 differentiation, and enumeration of Escherichia coli and other coliforms from environmental surfaces (E. coli D/E Neutralizing Agar turns blue, coliforms turn red). For the enumeration of environmental organisms. 15x60mm plate contact plate, The media is able to neutralize most antiseptics 10/pk ................................................................................ 89407-354 and disinfectants that may inhibit the growth of environmental organisms. Malt Extract 15x60mm contact plate, Malt Extract is recommended for the cultivation and 10/pk ................................................................................89407-482
  • View Our Full Water Sampling Vials Product Offering

    View Our Full Water Sampling Vials Product Offering

    TABLE OF CONTENTS Environmental Monitoring 1 Sample Prep and Dilution 8 Dehydrated Culture Media - Criterion™ 12 Prepared Culture Media 14 Chromogenic Media - HardyCHROM™ 18 CompactDry™ 20 Quality Control 24 Membrane Filtration 25 Rapid Tests 26 Lab Supplies/Sample Collection 27 Made in the USA Headquarters Distribution Centers 1430 West McCoy Lane Santa Maria, California Santa Maria, CA 93455 Olympia, Washington 800.266.2222 : phone Salt Lake City, Utah [email protected] Phoenix, Arizona HardyDiagnostics.com Dallas, Texas Springboro, Ohio Hardy Diagnostics has a Quality Lake City, Florida Management System that is certified Albany, New York to ISO 13485 and is a FDA licensed Copyright © 2020 Hardy Diagnostics Raleigh, North Carolina medical device manufacturer. Environmental Monitoring Hardy Diagnostics offers a wide selection of quality products intended to help keep you up to date with regulatory compliance, ensure the efficacy of your cleaning protocol, and properly monitor your environment. 800.266.2222 [email protected] HardyDiagnostics.com 1 Environmental Monitoring Air Sampling TRIO.BAS™ Impact Air Samplers introduced the newest generation of microbial air sampling. These ergonomically designed instruments combine precise air sampling with modern connectivity to help you properly assess the air quality of your laboratory and simplify your process. MONO DUO Each kit includes: Each kit includes: TRIO.BAS™ MONO air sampler, induction TRIO.BAS™ DUO air sampler, battery battery charger and cable, aspirating charger
  • BD Industry Catalog

    BD Industry Catalog

    PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80
  • Harmonized Pharmacopeia Dehydrated Media

    Harmonized Pharmacopeia Dehydrated Media

    Harmonised Pharmacopoeia Compliant to Dehydrated Culture Media EP 10th Edition 1. Sterility Testing Sterility testing is required when developing and manufacturing products for pharmaceutical applications as part of a sterilisation validation process as well as routine testing before release. Manufacturers must provide adequate and reliable sterility test data to ensure their product meets strict safety guidelines. Neogen offers the below media for sterility testing, which have been developed in accordance with the European Pharmacopoeia (EP) compliance guidelines: Fluid Thioglycollate Medium NCM0108 This medium has been designed for the detection of aerobic and anaerobic organisms including Clostridia spp., Pseudomonas spp. and Staphylococci. The medium has a nutritionally rich base to support the growth of a wide range of organisms as well as low oxygen reduction potential to prevent any species that may have a negative effect on the recovery and growth of contaminants. NCM0108 Tryptic Soy Broth (Soybean-Casein Digest Broth) NCM0004 This is a highly nutritious medium for the cultivation of a wide range of microorganisms including Aspergillus spp., Bacillus spp. and Candida spp. This versatile medium promotes growth of both fungi and aerobic bacteria, and can also be used as a pre-enrichment broth for non-sterile products. NCM0004 2. Examination of Non-Sterile Products Not all products which are released to market are required to be sterile. Instead, to guarantee that the product meets safety and quality standards, manufacturers are required to evaluate the microbial content of each product and ensure no organisms of concern are present. Neogen’s media range for the examination of non-sterile products has been developed for detection and enumeration of each organism specified within the HP including: Bile-Tolerant Gram-Negative Bacteria Enterobacteriaceae Enrichment (EE) Broth Mossel NCM0057 This is a selective broth for the enrichment of Enterobacteriaceae.
  • Cave Silver’ Biofilms on Rocks in the Former Homestake Mine in South Dakota, USA Amanpreet K

    Cave Silver’ Biofilms on Rocks in the Former Homestake Mine in South Dakota, USA Amanpreet K

    International Journal of Speleology 48 (2) 145-154 Tampa, FL (USA) May 2019 Available online at scholarcommons.usf.edu/ijs International Journal of Speleology Off icial Journal of Union Internationale de Spéléologie Culture-based analysis of ‘Cave Silver’ biofilms on Rocks in the former Homestake mine in South Dakota, USA Amanpreet K. Brar1 and David Bergmann2* 1Florida State University, 319 Stadium Dr., Tallahassee, FL 32306-4295, USA 2Black Hills State University, 1200 W. University St., Spearfish, SD 57789, USA Abstract: Tunnels in a warm, humid area of the 1478 m level of the Sanford Underground Research Facility (SURF), located in a former gold mine in South Dakota, USA, host irregular, thin whitish, iridescent biofilms, which appear superficially similar to ‘cave silver’ biofilms described from limestone and lava tube caves, despite the higher rock temperature (32°C) and differing rock surface (phyllite) present at SURF. In this study, we investigated the diversity of cultivable bacteria constituting the cave silver by using several media: CN agar, CN gellan gum and 0.1X R2A agar. The highest colony count (CFU/g of sample) was observed on 0.1X R2A medium. The bacterial strains were grouped into 39 distinct genotypes by randomly amplified polymorphic DNA (RAPD) analysis. In addition, the bacterial strains were further characterized based on their phenotypic and biochemical properties. 16S rRNA gene sequencing classified the cave silver isolates into three major bacterial phyla: Proteobacteria, Actinobacteria and Firmicutes. Isolates included some known genera; such as Taonella, Dongia, Mesorhizobium, Ralstonia, Pedomicrobium, Bauldia, Pseudolabrys, Reyrnella, Mizugakiibacter, Bradyrhizobium, Pseudomonas, Micrococcus, Sporichthya, Allokutzneria, Amycolatopsis, Pseudonocardia, and Paenibacillus.
  • CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)

    CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)

    Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Difco Manual Preface.ind 1 3/16/09 3:02:34 PM Table of Contents Contents Preface ...............................................................................................................................................................v About This Manual ...........................................................................................................................................vii History of BD Diagnostics .................................................................................................................................ix Section I: Monographs .......................................................................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media ..................................................................................................................5 Culture Media Ingredients – Agars ...................................................................................................................6
  • Anaerobe Agar

    Anaerobe Agar

    Thermo Scientific Microbiology Prepared media selection guide For the isolation, identification, differentiation and susceptibility testing of microorganisms Table of Contents Anaerobe Agar 4 Antimicrobial Susceptibility Testing (AST) Agars 6 Bi-plates 9 Blood Agars 14 Brilliance Chromogenic Media (Clinical) 16 Brilliance Chromogenic Media (Food) 21 Diluents, Water & Peptones 23 Dip-Slides 24 General Purpose Media 25 Pharmaceutical Media 29 ReadyBags 32 Water Testing 33 Culture Media byby Organism Type Aeromonas 36 ListeriaListeria 5252 Bacillus cereus 366 MycoplasmaMycoplasma / Ureaplasma 52 Bordetella 37 PasteurellaPasteurella 5353 Burkholderia cepaciaacia 37 Pseudomonas 53 Campylobacter 388 SalmonellaSalmonella 5454 Clostridium specieses 38 Staphylococci / Streptococcii 5757 Coliforms / Escherichiarichia colii 4040 StaphylococcusStaphylococcus aureusaureus 5858 Corynebacteria 411 StreptococcusStreptococcus agalactiaeagalactiae 5959 Dermatophytes 41 Trichomonas 60 Escherichia coli O157 42 Vibrio 60 Enterobacteriaceae 43 Yeasts & Moulds 61 Enterococci 47 Yersinia 63 Gardnerella 48 Haemophilus & Neisseria 48 Helicobacter pylori 50 Lactobacilli / Bifidobacteria 50 Legionella 51 Bringing more to microbiology Highest levels of quality & consistency Our culture medium expertise and rigorous quality standards have made us a preferred supplier and trusted source of prepared media to laboratories around the world. With a full range of formulations and formats, our media products combine ease-of-use with accurate, reproducible performance. You can rest assured knowing the Thermo Scientific™ culture media that reaches your benchtop will provide optimal recovery and differentiation of organisms, for greater confidence in your results. Unmatched service & support When you choose Thermo Scientific products for your microbiology needs, consider it the start of a lifelong partnership. Whether you need assistance with protocols, product transitions or product troubleshooting, our team of experts is ready to help you.
  • A Genomic and Proteomic Characterization of the First Cultured Oligotrophic Marine Gammaproteobacterium from the SAR92 Clade

    A Genomic and Proteomic Characterization of the First Cultured Oligotrophic Marine Gammaproteobacterium from the SAR92 Clade

    AN ABSTRACT OF THE THESIS OF Brett L. Mellbye for the degree of Honors Baccalaureate of Science in Microbiology presented on June 2, 2006. Title: A Genomic and Proteomic Characterization of the First Cultured Oligotrophic Marine Gammaproteobacterium from the SAR92 Clade. Abstract approved: _______________________________________________ Stephen J. Giovannoni High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain fell into the SAR92 clade in the oligotrophic marine Gammaproteobacteria (OMG) group. The OMG group is distantly related to previously cultivated genera of Gammaproteobacteria. Initial phylogenetic characterization was followed by genome sequencing and interpretation, proteomic analysis by liquid chromatography/tandem mass spectrometry, and determination of the fatty acid profile. Culture experiments, microscopic observations, and the genome sequence indicate that HTCC 2207 cells are motile, aerobic, heterotrophic, Gram-negative, short rods of approximately 0.148 µm3. Growth characteristics were observed at six different carbon concentrations and five different temperatures. Optimal growth rate (3.15 d-1) occurred at 16 ºC in natural seawater amended with nitrogen, phosphorus, vitamins, and a mixture of organic carbon compounds yielding a maximum cell density of 1.85 × 107 cells per ml. In contrast, the maximum cell density in seawater without addition organic carbon was 1.01 × 106 cells per ml. This strain has been described previously to form small colonies on 1/10 R2A agar media, but did not growth in any other artificial media.
  • Supplementary Information for Handbook of Culture Media for Food and Water Microbiology, 3Rd Edition © the Royal Society of Chemistry 2012

    Supplementary Information for Handbook of Culture Media for Food and Water Microbiology, 3Rd Edition © the Royal Society of Chemistry 2012

    Supplementary information for Handbook of Culture Media for Food and Water Microbiology, 3rd Edition © The Royal Society of Chemistry 2012 (a) (b) Figure 3.2 (a) Several C. perfringens strains in RPM (positive reaction left with coa- gulation and formation of a curd due to the fermentation of lactose and casein in Crossley milk by bacterial enzymes; negative reaction right). (b) Several C. perfringens strains in RCM (positive reaction left with coagu- lation of proteins in the Crossley milk acidification due to fermentation of lactose by bacterial enzymes, visible as colour change of the pH indicator bromocresol purple from purple to pale yellow; negative reaction right). DSM 2046 DSM 4312 DSM 2048 DSM 12443 B. thuringiensis B. cereus B. mycoides B. pseudomycoides Figure 4.2 Colony morphology of strains a liated to the B. cereus group on CEI (upper row), on PEMBA (middle row), and on Columbia blood agar (bottom row). Plates were incubated for 20ffi h at 36 °C. Supplementary information for Handbook of Culture Media for Food and Water Microbiology, 3rd Edition © The Royal Society of Chemistry 2012 7 >6.5 >5.5 6 5 4 3 log CFU/g 2 1 0 sample 1 sample 2 sample 3 sample 4 sample 5 sample 6 base Oxoid+RPF base Oxoid+egg yolk base Biokar+RPF base Biokar+egg yolk Figure 6.1 Influence of agar base brands on total CFU of BPA and RPFA (log CFU/g of six cheeses made from raw milk, 48 h at 37 °C) (Zangerl, 1999). (A) (B) Figure 10.2 Digital image of Bifidobacterium spp.
  • Neogen Culture Media

    Neogen Culture Media

    Neogen Culture Media Product Guide Edition 1, Nov 2019 Neogen Culture Media Contents About Neogen .......................................................3 Mycobacterium .....................................................27 View Our Culture Media by: Neisseria ..............................................................27 Organism ................................................................6 Proteus ................................................................28 Pseudomonas.......................................................29 Anaerobes ..............................................................6 Salmonella ...........................................................30 Bacillus cereus .......................................................7 Shigella ................................................................33 Brucella ..................................................................8 Spoilage ...............................................................33 Campylobacter .......................................................9 Staphylococcus ....................................................34 Candida ...............................................................10 Sterility Testing .....................................................35 Clostridia ..............................................................11 Streptococcus ......................................................35 Coliforms .............................................................13 UTI (Urinary Tract Infection) ....................................36