1 Trypsin isozymes in the lobster Panulirus argus (Latreille, 1804): from molecules 2 to physiology 3 4 E. Perera1,4*†, L. Rodríguez-Viera1, R. Perdomo-Morales2, V. Montero-Alejo2, F.J. Moyano3, G. 5 Martínez-Rodriguez4, J.M. Mancera5 6 7 1 Center for Marine Research, University of Havana, Havana, Cuba 8 2 Biochemistry Department, Center for Pharmaceuticals Research and Development, Havana, Cuba 9 3 Department of Applied Biology, University of Almeria, Almeria, Spain 10 4 Instituto de Ciencias Marinas de Andalucía (ICMAN), CSIC, Cádiz, Spain 11 5 Department of Biology, Faculty of Marine and Environmental Science, University of Cadiz, Spain † 12 * Corresponding author, Current address: Instituto de Ciencias Marinas de Andalucía (ICMAN), CSIC, Apartado Oficial, 11510- 13 Puerto Real, Cádiz, Spain. Tel. +34 956 832612 ext. 334, E-mail address:
[email protected];
[email protected] (E. 14 Perera). 15 16 Running title: Trypsins in lobster: from molecules to physiology 17 18 Abstract 19 Trypsin enzymes have been studied in a wide variety of animal taxa due to their central role in 20 protein digestion as well as in other important physiological and biotechnological processes. 21 Crustacean trypsins exhibit a high number of isoforms. However, while differences in properties 22 of isoenzymes are known to play important roles in regulating different physiological processes, 23 there is little information on this aspect for decapod trypsins. The aim of this review is to 24 integrate recent findings at the molecular level on trypsins enzymes of the spiny lobster 25 Panulirus argus, into higher levels of organization (biochemical, organism) and to interpret 26 those findings in relation to the feeding ecology of these crustaceans.