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Identification Et Caractérisation De Composés Produits Par Des Bactéries Environnementales Pour La Lutte Biologique Contre Legionella Pneumophila Marie-Hélène Corre

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Identification Et Caractérisation De Composés Produits Par Des Bactéries Environnementales Pour La Lutte Biologique Contre Legionella Pneumophila Marie-Hélène Corre Identification et caractérisation de composés produits par des bactéries environnementales pour la lutte biologique contre Legionella pneumophila Marie-Hélène Corre To cite this version: Marie-Hélène Corre. Identification et caractérisation de composés produits par des bactéries environ- nementales pour la lutte biologique contre Legionella pneumophila. Chimie analytique. Université de Poitiers, 2018. Français. NNT : 2018POIT2309. tel-02461235 HAL Id: tel-02461235 https://tel.archives-ouvertes.fr/tel-02461235 Submitted on 30 Jan 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. THESE Pour l’obtention du Grade de DOCTEUR DE L’UNIVERSITE DE POITIERS FACULTE DES SCIENCES FONDAMENTALES ET APPLIQUEES (Diplôme National - Arrêté du 25 mai 2016) Ecole Doctorale : Gay Lussac – Sciences pour l'environnement Secteur de Recherche : Aspects moléculaires et cellulaires de la biologie - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Identification et caractérisation de composés produits par des bactéries environnementales pour la lutte biologique contre Legionella pneumophila - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Présentée par : Marie-Hélène CORRE Directeurs de Thèse : Jean-Marc BERJEAUD, Professeur, Université de Poitiers Julien VERDON, Maître de Conférences, Université de Poitiers Soutenue le 10 Décembre 2018 devant la Commission d’Examen Rapporteurs : Ali LADRAM, Professeur, Sorbonne Université Isabelle SCHALK, Directeur de recherche CNRS, Strasbourg Examinateurs : Sylvie CHEVALIER, Professeur, Université de Rouen Sophie LECOMTE, Directeur de recherche CNRS, Bordeaux Christophe GINEVRA, Docteur, Université de Lyon Julien VERDON, Maître de Conférences, Université de Poitiers Jean-Marc BERJEAUD, Professeur, Université de Poitiers THESE Pour l’obtention du Grade de DOCTEUR DE L’UNIVERSITE DE POITIERS FACULTE DES SCIENCES FONDAMENTALES ET APPLIQUEES (Diplôme National - Arrêté du 25 mai 2016) Ecole Doctorale : Gay Lussac – Sciences pour l'environnement Secteur de Recherche : Aspects moléculaires et cellulaires de la biologie - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Identification et caractérisation de composés produits par des bactéries environnementales pour la lutte biologique contre Legionella pneumophila - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Présentée par : Marie-Hélène CORRE Directeurs de Thèse : Jean-Marc BERJEAUD, Professeur, Université de Poitiers Julien VERDON, Maître de Conférences, Université de Poitiers Soutenue le 10 Décembre 2018 devant la Commission d’Examen Rapporteurs : Ali LADRAM, Professeur, Sorbonne Université Isabelle SCHALK, Directeur de recherche CNRS, Strasbourg Examinateurs : Sylvie CHEVALIER, Professeur, Université de Rouen Sophie LECOMTE, Directeur de recherche CNRS, Bordeaux Christophe GINEVRA, Docteur, Université de Lyon Julien VERDON, Maître de Conférences, Université de Poitiers Jean-Marc BERJEAUD, Professeur, Université de Poitiers Dans la vie, rien est à craindre, tout est à comprendre Marie Curie À ma famille, Remerciements Je n’aurai pu réaliser cette thèse sans la générosité, les conseils et la bonne humeur d’un très grand nombre de personnes. J’aimerai donc les remercier d’avoir participé à ce travail et de m’avoir soutenue pendant ces trois années. Tout d’abord, je souhaite adresser ma gratitude à Didier Bouchon (ancien directeur du laboratoire EBI) et à Jean-Marc Berjeaud (directeur actuel) pour m’avoir permise de développer ce travail de thèse au sein de leur laboratoire. J’aimerai remercier Isabelle Schalk et Ali Ladram, rapporteurs de ce manuscrit, pour m’avoir fait l’honneur d’évaluer ce travail. Je souhaite également remercier Sophie Lecomte, Christophe Ginevra et Sylvie Chevalier pour avoir bien voulu faire partie de ce jury de thèse. Encore une fois, je tiens à remercier très sincèrement Jean-Marc Berjeaud, directeur de la thèse, pour toute la confiance qu’il m’a accordé pour mener à bien ce projet. Je lui suis reconnaissante pour sa disponibilité, pour son écoute, pour ses conseils et pour les opportunités qu’il a su m’offrir (ma participation à l’ESGLI, au GDR MufoPAM). Je lui exprime également ma gratitude pour son accompagnement motivationnel lors de ma présentation à MT180. Je tiens à le remercier aussi pour ses grandes qualités humaines, sa franchise et ses valeurs. J’exprime mes remerciements à Julien Verdon, co-directeur de cette thèse, sans qui ce projet n’aurait pas pu être mis en place. Je le remercie pour toutes les opportunités qu’il a su m’offrir pendant ces trois années (la Gordon en Californie, les projets secondaires, les collaborations). Nous avons beaucoup voyagé scientifiquement tous les deux. Un de nos plus beaux moments restera aussi un des plus douloureux : la randonnée au parc Griffith de Los Angeles... Je le remercie également pour son aide pour la valorisation scientifique associée à ce travail et pour son aide dans les moments de stress. Nous avons bien rigolé tous les trois, MERCI. J’aimerai que vous sachiez tous les deux que je me suis vraiment amusée pendant ces trois années ! Je remercie les membres de mon comité de suivi de thèse, Ali Ladram, Anabelle Merieau, Richard Cordaux et Sophie Lecomte pour leur présence à mi-parcours et leurs conseils. J’aimerai remercier chaleureusement l’Institut de Recherche sur la Biologie de l’Insecte (IRBI) à Tours et particulièrement David Giron et Ali Khalil (désolée pour le coup de la panne), pour notre collaboration et pour m’avoir accueillie dans votre laboratoire. Je souhaite remercier Vincent Delafont pour sa générosité. Tu m'as beaucoup aidée dans mon travail de thèse, par ton écoute et tes conseils. Je te remercie aussi pour la relecture du manuscrit et pour la valorisation de la collection. Enfin, merci pour les pauses clopes, toujours accompagnées de bonne humeur ! Je remercie également Emilie Portier pour avoir bien voulu relire une partie du manuscrit. J'aimerai remercier très sincèrement Laurence Belligot, Sylvie Clercy-Morel et Karine Demangeau pour toute la gestion administrative autour de ce projet et autour de la vie de laboratoire. Rien ne tournerai sans vous, vous êtes formidables et attentionnées et on peut toujours compter sur vous, y compris dans l'urgence. Je tiens à remercier Daniel Guyonnet, pour sa disponibilité pour passer mes séquences, pour son écoute, les pauses café, les petits déjeuners. Tu as toujours été là pour m’écouter me plaindre et ça n'a pas dû être facile tous les jours. Alors merci à toi, pour tes valeurs humaines, ton humour, ton implication au laboratoire et ta bienveillance envers moi. Thomas Becking, j’aimerai te remercier pour les moments que tu m’as accordés et pour nos échanges. Mais plus encore je te remercie pour tes qualités humaines, ton ouverture d’esprit, ton soutien et ton écoute lors de cette dernière année. Je souhaite remercier Benoit Porcheron, pour sa grande disponibilité pour passer mes échantillons au lyoph et pour sa gentillesse. Je désire également remercier Willy Aucher, pour tout le temps qu'il a consacré à mes questions et pour ses conseils. J'exprime ma gratitude envers Christine Braquart-Varnier et Marie-Hélène Rodier pour leurs attentions envers moi. Merci également pour votre humour contagieux. Je remercie mes compagnons de bureau, Clémence Loiseau lors de ma première année et Alexandre Crepin pour le reste de la thèse. J’ai passé de bons moments avec vous. Merci Alex pour les relectures, et les critiques sur certains de mes travaux. On a bien rigolé ! Je tiens à remercier Dominique Beguin pour toutes les discussions que nous avons eu pendant la première année. Tous les matins à 7h30, j'avais mon petit bonjour dans le bureau. Tu as été d'une grande écoute et d'un bon soutien pour moi, alors je te remercie du fond du cœur pour ça. J'aimerai remercier Christophe Souil, pour tout le travail qu'il a fait pour nous permettre de manipuler dans de bonnes conditions et pour la préparation des milieux de culture. Je désire également remercier chaleureusement François Baty-Sorel pour son écoute et ses conseils, de même que pour ses formations très enrichissantes. Je lui suis reconnaissante pour son accompagnement pour « ma thèse en 180 secondes ». Je remercie Stéphanie Crapart et Céline Lebon pour tout leur travail associé à la préparation des salles de TPs. J'aimerai également dire merci à Stéphanie pour sa grande efficacité au laboratoire. J’aimerai exprimer ma gratitude envers les étudiants de Licence Sciences du Vivant (2016-2019) et les Master 1 PNBCM (2016-2019) de l’Université de Poitiers pour m’avoir accompagnée dans le développement de ma pédagogie universitaire. J'aimerai également dire merci à Camille Juin avec qui j'ai tellement partagé d'émotions.. de la tristesse, de la joie (l'annonce du bébé à côté du plus bel appareil du laboratoire), c'était super ! Merci de m'avoir écouté
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