PRINT DATE: 2021-10-05 12:30:28 +0200

JOB ID: 29ae139a-3a41-4571-a19b-809af76a3ec5

RESULT PAGE: https://tygs.dsmz.de/user_results/show?guid=29ae139a-3a41-4571-a19b-809af76a3ec5

Table 1: Phylogenies Publication-ready versions of both the genome-scale GBDP tree and the 16S rRNA gene sequence tree can be customized and exported either in SVG (vector graphic) or PNG format from within the phylogeny viewers in your TYGS result page. For publications the SVG format is recommended because it is lossless, always keeps its high resolution and can also be easily converted to other popular formats such as PDF or EPS. Please follow the link provided above!

Table 2: Identification The below list contains the result of the TYGS species identification routine.

Explanation of remarks that might occur in the below table:

remark [R1]: The TYGS type strain database is automatically updated on an almost daily basis. However, if a particular type strain genome is not available in the TYGS database, this can have several reasons which are detailed in the FAQ. You can request an extended 16S rRNA gene analysis via the 16S tree viewer found in your result page to detect not yet genome-sequenced type strains relevant for your study.

remark [R2]: > 70% dDDH value (formula d4) and (almost) minimal dDDH values for gene-content formulae d0 and d6 indicate a potentially unreliable identification result and should thus be checked via the 16S rRNA gene sequence similarity. Such strong deviations can, in principle, be caused by sequence contamination.

remark [R3]: G+C content difference of > 1 % indicates a potentially unreliable identification result because within species G+C content varies no more than 1 %, if computed from genome sequences (PMID: 24505073).

Strain Conclusion Identification result Remark

'Legionella septentrionalis' (RZGR00000000) belongs to known species Legionella septentrionalis

'Legionella septentrionalis' (RZGS00000000) belongs to known species Legionella septentrionalis

'Legionella septentrionalis' (RZGT00000000) belongs to known species Legionella septentrionalis

'Legionella septentrionalis' (RZGV00000000) belongs to known species Legionella septentrionalis

'Legionella sp. km535' (RZGU00000000) belongs to known species Legionella moravica

'Legionella qingyii' (QHJG00000000) belongs to known species Legionella qingyii

'Legionella qingyii' (RZGW00000000) belongs to known species Legionella qingyii

'Legionella qingyii' (RZGX00000000) belongs to known species Legionella qingyii

'Legionella sp. km772' (RZGQ00000000) potential new species see [R1]

'Legionella sainthelensi' (UFAL00000000) potential new species see [R1]

'Legionella sainthelensi' (UFAM00000000) potential new species see [R1] Table 3: Pairwise comparisons of user genomes vs. type-strain genomes The following table contains the pairwise dDDH values between your user genomes and the selected type- strain genomes. The dDDH values are provided along with their confidence intervals (C.I.) for the three different GBDP formulas:

• formula d0 (a.k.a. GGDC formula 1): length of all HSPs divided by total genome length • formula d4 (a.k.a. GGDC formula 2): sum of all identities found in HSPs divided by overall HSP length • formula d6 (a.k.a. GGDC formula 3): sum of all identities found in HSPs divided by total genome length

Note: Formula d4 is independent of genome length and is thus robust against the use of incomplete draft genomes. For other reasons for preferring formula d4, see the FAQ.

Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella qingyii CCTCC 100.0 [100.0 - 100.0 [100.0 - 100.0 [100.0 - 0.01 (QHJG00000000) AB 2018025 100.0] 100.0] 100.0]

'Legionella Legionella septentrionalis 100.0 [100.0 - 100.0 [100.0 - 100.0 [100.0 - 0.03 septentrionalis' km711T 100.0] 100.0] 100.0] (RZGS00000000)

'Legionella qingyii' Legionella qingyii CCTCC 85.0 [81.3 - 99.9 [99.8 - 90.3 [87.6 - 0.02 (RZGX00000000) AB 2018025 88.1] 100.0] 92.5]

'Legionella qingyii' 'Legionella qingyii' 85.0 [81.3 - 99.9 [99.8 - 90.3 [87.6 - 0.02 (QHJG00000000) (RZGX00000000) 88.1] 100.0] 92.5]

'Legionella qingyii' 'Legionella qingyii' 98.5 [97.5 - 98.3 [97.5 - 99.2 [98.7 - 0.0 (RZGW00000000) (RZGX00000000) 99.2] 98.8] 99.5]

'Legionella qingyii' 'Legionella qingyii' 80.4 [76.5 - 97.9 [97.0 - 86.2 [83.1 - 0.02 (QHJG00000000) (RZGW00000000) 83.8] 98.5] 88.9]

'Legionella qingyii' Legionella qingyii CCTCC 80.4 [76.5 - 97.9 [97.0 - 86.2 [83.1 - 0.02 (RZGW00000000) AB 2018025 83.8] 98.5] 88.9]

'Legionella 'Legionella 91.8 [88.8 - 97.3 [96.2 - 94.8 [92.8 - 0.17 septentrionalis' septentrionalis' 94.0] 98.1] 96.3] (RZGS00000000) (RZGT00000000)

'Legionella Legionella septentrionalis 91.8 [88.8 - 97.3 [96.2 - 94.8 [92.8 - 0.2 septentrionalis' km711T 94.0] 98.1] 96.3] (RZGT00000000)

'Legionella 'Legionella 93.2 [90.5 - 97.2 [96.1 - 95.8 [94.1 - 0.02 septentrionalis' septentrionalis' 95.2] 98.0] 97.1] (RZGT00000000) (RZGV00000000)

'Legionella 'Legionella 92.1 [89.1 - 96.6 [95.4 - 94.9 [93.0 - 0.13 septentrionalis' septentrionalis' 94.3] 97.5] 96.4] (RZGR00000000) (RZGT00000000)

'Legionella 'Legionella 90.1 [86.9 - 96.2 [94.8 - 93.5 [91.2 - 0.16 septentrionalis' septentrionalis' 92.6] 97.2] 95.2] (RZGS00000000) (RZGV00000000)

'Legionella Legionella septentrionalis 90.1 [86.9 - 96.2 [94.8 - 93.5 [91.2 - 0.19 septentrionalis' km711T 92.6] 97.2] 95.2] (RZGV00000000)

'Legionella 'Legionella 93.0 [90.2 - 94.5 [92.8 - 95.3 [93.4 - 0.11 septentrionalis' septentrionalis' 95.0] 95.8] 96.6] (RZGR00000000) (RZGV00000000)

'Legionella 'Legionella 90.3 [87.1 - 94.5 [92.8 - 93.4 [91.1 - 0.04 septentrionalis' septentrionalis' 92.8] 95.8] 95.1] (RZGR00000000) (RZGS00000000)

'Legionella Legionella septentrionalis 90.3 [87.1 - 94.5 [92.8 - 93.4 [91.1 - 0.07 septentrionalis' km711T 92.8] 95.8] 95.1] (RZGR00000000)

'Legionella sainthelensi' 'Legionella sainthelensi' 83.1 [79.2 - 72.7 [69.7 - 84.1 [80.9 - 0.06 (UFAL00000000) (UFAM00000000) 86.3] 75.5] 86.9]

'Legionella sp. km535' Legionella moravica DSM 76.7 [72.7 - 72.2 [69.2 - 78.7 [75.2 - 0.12 (RZGU00000000) 19234 80.3] 75.1] 81.8]

'Legionella sainthelensi' Legionella sainthelensi 88.6 [85.2 - 69.4 [66.4 - 88.0 [85.0 - 0.19 (UFAL00000000) ATCC 35248 91.3] 72.3] 90.4] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella sainthelensi' Legionella sainthelensi 78.9 [74.9 - 66.5 [63.6 - 79.3 [75.9 - 0.13 (UFAM00000000) ATCC 35248 82.3] 69.4] 82.3]

'Legionella qingyii' Legionella gormanii ATCC 76.6 [72.6 - 45.2 [42.6 - 71.0 [67.5 - 0.26 (RZGX00000000) 33297 80.2] 47.7] 74.2]

'Legionella qingyii' Legionella gormanii ATCC 76.4 [72.5 - 45.2 [42.6 - 70.9 [67.4 - 0.26 (RZGX00000000) 33342 80.0] 47.8] 74.1]

'Legionella qingyii' Legionella gormanii ATCC 59.7 [56.1 - 45.2 [42.7 - 57.3 [54.1 - 0.28 (QHJG00000000) 33342 63.3] 47.8] 60.4]

'Legionella qingyii' Legionella gormanii ATCC 78.3 [74.4 - 45.2 [42.6 - 72.4 [68.9 - 0.26 (RZGW00000000) 33342 81.9] 47.7] 75.6]

'Legionella qingyii' Legionella gormanii ATCC 59.9 [56.2 - 45.2 [42.6 - 57.4 [54.2 - 0.27 (QHJG00000000) 33297 63.4] 47.8] 60.6]

'Legionella qingyii' Legionella gormanii ATCC 78.6 [74.6 - 45.1 [42.6 - 72.6 [69.1 - 0.25 (RZGW00000000) 33297 82.1] 47.7] 75.8]

'Legionella sainthelensi' Legionella santicrucis 53.4 [49.9 - 38.3 [35.8 - 49.9 [46.8 - 0.19 (UFAM00000000) ATCC 35301 56.8] 40.8] 52.9]

'Legionella sainthelensi' Legionella longbeachae 62.2 [58.5 - 37.5 [35.1 - 56.5 [53.3 - 0.21 (UFAL00000000) NCTC 11477 65.8] 40.0] 59.6]

'Legionella sainthelensi' Legionella santicrucis 54.6 [51.1 - 37.3 [34.9 - 50.5 [47.5 - 0.13 (UFAL00000000) ATCC 35301 58.1] 39.8] 53.6]

'Legionella sainthelensi' Legionella cincinnatiensis 57.5 [53.9 - 37.1 [34.6 - 52.7 [49.6 - 0.01 (UFAL00000000) ATCC 43753 61.0] 39.6] 55.7]

'Legionella sainthelensi' Legionella longbeachae 55.7 [52.1 - 36.6 [34.2 - 51.1 [48.0 - 0.15 (UFAM00000000) NCTC 11477 59.2] 39.1] 54.2]

'Legionella sainthelensi' Legionella cincinnatiensis 53.8 [50.2 - 36.5 [34.0 - 49.6 [46.5 - 0.07 (UFAM00000000) ATCC 43753 57.2] 39.0] 52.6]

'Legionella Legionella fairfieldensis 13.5 [10.7 - 35.4 [32.9 - 13.9 [11.5 - 2.56 septentrionalis' ATCC 49588 16.8] 37.9] 16.7] (RZGR00000000)

'Legionella Legionella 13.4 [10.6 - 35.0 [32.6 - 13.8 [11.4 - 2.79 septentrionalis' jamestowniensis DSM 16.7] 37.6] 16.6] (RZGR00000000) 19215

'Legionella Legionella santicrucis 13.1 [10.3 - 33.3 [30.9 - 13.5 [11.1 - 5.55 septentrionalis' ATCC 35301 16.4] 35.8] 16.3] (RZGR00000000)

'Legionella Legionella gratiana ATCC 13.2 [10.5 - 31.5 [29.1 - 13.6 [11.2 - 5.47 septentrionalis' 49413 16.5] 34.0] 16.4] (RZGV00000000)

'Legionella sainthelensi' Legionella gratiana ATCC 37.6 [34.2 - 31.3 [28.9 - 35.2 [32.3 - 0.0 (UFAM00000000) 49413 41.1] 33.8] 38.3]

'Legionella Legionella rubrilucens 13.5 [10.8 - 30.9 [28.5 - 13.9 [11.5 - 5.45 septentrionalis' ATCC 35304 16.9] 33.4] 16.7] (RZGR00000000)

'Legionella Legionella norrlandica 13.3 [10.6 - 30.2 [27.8 - 13.7 [11.4 - 4.69 septentrionalis' LEGN 16.7] 32.7] 16.5] (RZGS00000000)

'Legionella sainthelensi' Legionella gratiana ATCC 36.9 [33.5 - 30.1 [27.7 - 34.3 [31.4 - 0.07 (UFAL00000000) 49413 40.4] 32.6] 37.4]

'Legionella Legionella brunensis 13.1 [10.4 - 29.6 [27.2 - 13.5 [11.2 - 3.54 septentrionalis' ATCC 43878 16.4] 32.1] 16.3] (RZGR00000000)

'Legionella Legionella norrlandica 13.2 [10.5 - 29.2 [26.8 - 13.6 [11.2 - 4.84 septentrionalis' LEGN 16.5] 31.7] 16.4] (RZGV00000000)

'Legionella Legionella jordanis ATCC 13.4 [10.6 - 28.5 [26.1 - 13.8 [11.4 - 0.48 septentrionalis' 33623 16.7] 31.0] 16.6] (RZGS00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella sainthelensi' Legionella hackeliae 13.4 [10.6 - 28.3 [26.0 - 13.8 [11.4 - 2.12 (UFAM00000000) ATCC 35250 16.7] 30.8] 16.6]

'Legionella Legionella parisiensis 13.0 [10.3 - 28.3 [25.9 - 13.4 [11.1 - 4.34 septentrionalis' DSM 19216 16.3] 30.8] 16.2] (RZGV00000000)

'Legionella sp. km535' Legionella quateirensis 36.5 [33.1 - 28.0 [25.7 - 33.5 [30.5 - 0.88 (RZGU00000000) ATCC 49507 40.0] 30.5] 36.6]

'Legionella sainthelensi' Legionella erythra ATCC 13.0 [10.3 - 27.8 [25.4 - 13.4 [11.0 - 9.85 (UFAM00000000) 35303 16.3] 30.2] 16.1]

'Legionella Legionella pneumophila 13.3 [10.5 - 27.6 [25.2 - 13.7 [11.3 - 3.89 septentrionalis' Philadelphia 1 16.6] 30.0] 16.5] (RZGS00000000)

'Legionella qingyii' Legionella bozemanae 26.4 [23.1 - 27.5 [25.2 - 25.4 [22.5 - 0.11 (QHJG00000000) ATCC 33217 30.1] 30.0] 28.5]

'Legionella qingyii' Legionella bozemanae 29.7 [26.3 - 27.4 [25.0 - 28.0 [25.1 - 0.09 (RZGX00000000) ATCC 33217 33.3] 29.9] 31.1]

'Legionella qingyii' Legionella bozemanae 28.8 [25.5 - 27.2 [24.8 - 27.3 [24.4 - 0.09 (RZGW00000000) ATCC 33217 32.5] 29.7] 30.4]

'Legionella sainthelensi' Legionella quateirensis 13.9 [11.1 - 27.2 [24.8 - 14.2 [11.8 - 2.16 (UFAM00000000) ATCC 49507 17.2] 29.7] 17.1]

'Legionella qingyii' Legionella anisa ATCC 27.9 [24.6 - 27.0 [24.7 - 26.6 [23.7 - 0.37 (RZGW00000000) 35292 31.6] 29.5] 29.7]

'Legionella qingyii' Legionella anisa ATCC 25.3 [22.0 - 27.0 [24.7 - 24.4 [21.6 - 0.38 (QHJG00000000) 35292 29.0] 29.5] 27.5]

'Legionella qingyii' Legionella anisa ATCC 27.5 [24.2 - 27.0 [24.6 - 26.2 [23.4 - 0.37 (RZGX00000000) 35292 31.2] 29.5] 29.4]

'Legionella sainthelensi' Legionella anisa ATCC 17.4 [14.4 - 26.9 [24.6 - 17.5 [14.9 - 1.34 (UFAM00000000) 35292 21.0] 29.4] 20.5]

'Legionella Legionella clemsonensis 13.2 [10.5 - 26.9 [24.6 - 13.6 [11.2 - 2.95 septentrionalis' CDC-D5610 16.6] 29.4] 16.4] (RZGV00000000)

'Legionella qingyii' Legionella tucsonensis 31.8 [28.4 - 26.8 [24.4 - 29.6 [26.6 - 0.41 (RZGW00000000) ATCC 49180 35.3] 29.3] 32.7]

'Legionella qingyii' Legionella tucsonensis 27.5 [24.2 - 26.8 [24.5 - 26.2 [23.3 - 0.39 (QHJG00000000) ATCC 49180 31.1] 29.3] 29.3]

'Legionella qingyii' Legionella tucsonensis 31.3 [27.9 - 26.8 [24.4 - 29.2 [26.2 - 0.41 (RZGX00000000) ATCC 49180 34.9] 29.3] 32.3]

'Legionella sainthelensi' Legionella parisiensis 17.0 [14.0 - 26.5 [24.2 - 17.1 [14.5 - 1.13 (UFAM00000000) DSM 19216 20.5] 29.0] 20.1]

'Legionella sainthelensi' Legionella dumoffii NY 23 16.4 [13.4 - 26.4 [24.1 - 16.6 [14.0 - 2.8 (UFAM00000000) 19.9] 28.9] 19.5]

'Legionella qingyii' Legionella parisiensis 27.3 [23.9 - 26.3 [23.9 - 25.9 [23.0 - 0.15 (RZGW00000000) DSM 19216 30.9] 28.8] 29.0]

'Legionella qingyii' Legionella parisiensis 24.6 [21.3 - 26.3 [23.9 - 23.7 [20.9 - 0.17 (QHJG00000000) DSM 19216 28.3] 28.8] 26.8]

'Legionella qingyii' Legionella parisiensis 26.9 [23.6 - 26.2 [23.8 - 25.6 [22.7 - 0.16 (RZGX00000000) DSM 19216 30.6] 28.7] 28.7]

'Legionella Legionella pneumophila 13.3 [10.6 - 26.1 [23.8 - 13.7 [11.3 - 4.23 septentrionalis' subsp. pascullei ATCC 16.6] 28.6] 16.5] (RZGV00000000) 33737

'Legionella qingyii' Legionella steigerwaltii 26.3 [23.0 - 26.0 [23.6 - 25.1 [22.2 - 0.44 (RZGX00000000) ATCC 35302 30.0] 28.5] 28.2]

'Legionella qingyii' Legionella steigerwaltii 24.0 [20.7 - 26.0 [23.6 - 23.2 [20.4 - 0.46 (QHJG00000000) ATCC 35302 27.7] 28.5] 26.3]

'Legionella Legionella fallonii LLAP- 12.7 [10.0 - 25.9 [23.6 - 13.1 [10.7 - 3.99 septentrionalis' 10 15.9] 28.4] 15.8] (RZGV00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella pneumophila 13.3 [10.5 - 25.9 [23.6 - 13.6 [11.3 - 4.04 septentrionalis' Philadelphia 1 16.6] 28.4] 16.4] (RZGV00000000)

'Legionella qingyii' Legionella steigerwaltii 26.3 [23.0 - 25.7 [23.4 - 25.0 [22.2 - 0.44 (RZGW00000000) ATCC 35302 30.0] 28.2] 28.1]

'Legionella sainthelensi' Legionella gormanii ATCC 17.2 [14.1 - 25.6 [23.3 - 17.2 [14.6 - 1.23 (UFAM00000000) 33297 20.7] 28.1] 20.2]

'Legionella Legionella parisiensis 13.2 [10.4 - 25.6 [23.3 - 13.5 [11.2 - 4.18 septentrionalis' DSM 19216 16.5] 28.1] 16.3] (RZGS00000000)

'Legionella sainthelensi' Legionella gormanii ATCC 17.1 [14.1 - 25.5 [23.2 - 17.2 [14.6 - 1.24 (UFAM00000000) 33342 20.7] 28.0] 20.2]

'Legionella qingyii' Legionella cherrii DSM 22.5 [19.3 - 25.4 [23.0 - 21.9 [19.1 - 1.05 (QHJG00000000) 19213 26.2] 27.8] 25.0]

'Legionella qingyii' Legionella cherrii DSM 25.0 [21.6 - 25.3 [23.0 - 23.9 [21.0 - 1.04 (RZGW00000000) 19213 28.6] 27.8] 27.0]

'Legionella qingyii' Legionella cherrii DSM 24.7 [21.4 - 25.3 [23.0 - 23.7 [20.8 - 1.04 (RZGX00000000) 19213 28.3] 27.8] 26.8]

'Legionella sp. km772' Legionella santicrucis 13.5 [10.7 - 25.1 [22.7 - 13.8 [11.4 - 2.33 (RZGQ00000000) ATCC 35301 16.8] 27.5] 16.6]

'Legionella Legionella clemsonensis 13.3 [10.6 - 25.1 [22.8 - 13.7 [11.3 - 2.79 septentrionalis' CDC-D5610 16.6] 27.6] 16.5] (RZGS00000000)

'Legionella sainthelensi' Legionella saoudiensis 14.3 [11.5 - 25.0 [22.7 - 14.6 [12.1 - 2.58 (UFAM00000000) LH-SWC 17.7] 27.5] 17.4]

'Legionella sp. km772' Legionella drancourtii 13.7 [10.9 - 24.9 [22.6 - 14.0 [11.6 - 0.26 (RZGQ00000000) LLAP 12 17.0] 27.4] 16.8]

'Legionella qingyii' Legionella steelei ATCC 24.3 [21.0 - 24.9 [22.6 - 23.3 [20.5 - 0.95 (RZGX00000000) BAA-2169 28.0] 27.4] 26.4]

'Legionella qingyii' Legionella steelei ATCC 22.5 [19.3 - 24.9 [22.6 - 21.8 [19.0 - 0.96 (QHJG00000000) BAA-2169 26.2] 27.4] 24.9]

'Legionella qingyii' Legionella steelei ATCC 24.1 [20.8 - 24.8 [22.4 - 23.1 [20.3 - 0.94 (RZGW00000000) BAA-2169 27.7] 27.2] 26.2]

'Legionella Legionella sainthelensi 12.9 [10.2 - 24.7 [22.4 - 13.3 [10.9 - 5.23 septentrionalis' ATCC 35248 16.2] 27.2] 16.0] (RZGR00000000)

'Legionella sainthelensi' Legionella steigerwaltii 16.9 [13.9 - 24.6 [22.2 - 17.0 [14.4 - 1.42 (UFAM00000000) ATCC 35302 20.5] 27.0] 19.9]

'Legionella Legionella sainthelensi 13.0 [10.3 - 24.6 [22.3 - 13.4 [11.0 - 5.34 septentrionalis' ATCC 35248 16.3] 27.1] 16.2] (RZGV00000000)

'Legionella Legionella fallonii LLAP- 12.7 [10.0 - 24.5 [22.1 - 13.1 [10.7 - 3.88 septentrionalis' 10 15.9] 26.9] 15.8] (RZGR00000000)

'Legionella qingyii' Legionella hackeliae 13.1 [10.3 - 24.5 [22.2 - 13.4 [11.1 - 1.16 (QHJG00000000) ATCC 35250 16.4] 27.0] 16.2]

'Legionella qingyii' Legionella hackeliae 13.1 [10.4 - 24.4 [22.1 - 13.5 [11.1 - 1.15 (RZGX00000000) ATCC 35250 16.5] 26.9] 16.3]

'Legionella qingyii' Legionella pneumophila 13.1 [10.4 - 24.4 [22.1 - 13.5 [11.1 - 0.03 (QHJG00000000) subsp. fraseri ATCC 16.4] 26.9] 16.2] 33156

'Legionella Legionella fallonii LLAP- 12.7 [10.0 - 24.3 [22.0 - 13.1 [10.7 - 3.83 septentrionalis' 10 15.9] 26.8] 15.8] (RZGS00000000)

'Legionella Legionella dumoffii NY 23 13.0 [10.3 - 24.3 [22.0 - 13.4 [11.0 - 2.56 septentrionalis' 16.3] 26.8] 16.2] (RZGR00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella gratiana ATCC 13.1 [10.4 - 24.2 [21.9 - 13.5 [11.1 - 5.31 septentrionalis' 49413 16.4] 26.7] 16.3] (RZGS00000000)

'Legionella Legionella spiritensis 13.0 [10.3 - 24.2 [21.9 - 13.4 [11.0 - 3.18 septentrionalis' ATCC 35249 16.3] 26.7] 16.1] (RZGR00000000)

'Legionella qingyii' Legionella pneumophila 13.2 [10.5 - 24.2 [21.9 - 13.6 [11.2 - 0.01 (RZGX00000000) subsp. fraseri ATCC 16.5] 26.6] 16.4] 33156

'Legionella sainthelensi' Legionella 12.9 [10.2 - 24.2 [21.9 - 13.3 [11.0 - 6.62 (UFAM00000000) birminghamensis ATCC 16.2] 26.7] 16.1] 43702

'Legionella sainthelensi' Legionella pneumophila 13.2 [10.5 - 24.1 [21.8 - 13.6 [11.2 - 1.43 (UFAM00000000) Philadelphia 1 16.6] 26.5] 16.4]

'Legionella Legionella nagasakiensis 13.8 [11.0 - 24.0 [21.7 - 14.1 [11.7 - 1.09 septentrionalis' JCM 15315 17.1] 26.5] 16.9] (RZGS00000000)

'Legionella qingyii' Legionella beliardensis 12.7 [10.0 - 23.8 [21.5 - 13.0 [10.7 - 0.47 (QHJG00000000) NCTC 13315 15.9] 26.2] 15.8]

'Legionella qingyii' Legionella pneumophila 13.2 [10.5 - 23.8 [21.5 - 13.6 [11.2 - 0.01 (RZGW00000000) subsp. fraseri ATCC 16.5] 26.3] 16.3] 33156

'Legionella sainthelensi' Legionella bozemanae 16.5 [13.5 - 23.7 [21.4 - 16.5 [14.0 - 1.07 (UFAM00000000) ATCC 33217 20.0] 26.1] 19.5]

'Legionella sainthelensi' Legionella brunensis 13.0 [10.3 - 23.6 [21.3 - 13.3 [11.0 - 1.82 (UFAM00000000) ATCC 43878 16.3] 26.0] 16.1]

'Legionella Legionella waltersii ATCC 13.0 [10.3 - 23.6 [21.3 - 13.3 [11.0 - 3.13 septentrionalis' 51914 16.3] 26.1] 16.1] (RZGR00000000)

'Legionella sainthelensi' Legionella pneumophila 13.1 [10.4 - 23.5 [21.2 - 13.5 [11.1 - 0.99 (UFAM00000000) subsp. fraseri ATCC 16.5] 26.0] 16.3] 33156

'Legionella sp. km772' Legionella steelei ATCC 13.3 [10.5 - 23.4 [21.1 - 13.6 [11.2 - 0.21 (RZGQ00000000) BAA-2169 16.6] 25.9] 16.4]

'Legionella qingyii' Legionella saoudiensis 14.1 [11.3 - 23.4 [21.1 - 14.4 [12.0 - 1.62 (QHJG00000000) LH-SWC 17.5] 25.9] 17.2]

'Legionella sainthelensi' Legionella 13.0 [10.3 - 23.3 [21.0 - 13.4 [11.1 - 2.57 (UFAM00000000) jamestowniensis DSM 16.3] 25.7] 16.2] 19215

'Legionella sainthelensi' Legionella cherrii DSM 15.6 [12.7 - 23.3 [21.0 - 15.8 [13.2 - 2.08 (UFAL00000000) 19213 19.1] 25.8] 18.7]

'Legionella qingyii' 'Legionella sainthelensi' 16.3 [13.4 - 23.3 [21.0 - 16.4 [13.8 - 0.98 (RZGX00000000) (UFAM00000000) 19.8] 25.8] 19.3]

'Legionella sainthelensi' Legionella qingyii CCTCC 15.9 [13.0 - 23.3 [21.0 - 16.0 [13.5 - 0.95 (UFAM00000000) AB 2018025 19.4] 25.7] 18.9]

'Legionella sp. km535' Legionella sainthelensi 13.6 [10.8 - 23.3 [21.0 - 13.9 [11.5 - 2.91 (RZGU00000000) ATCC 35248 16.9] 25.8] 16.7]

'Legionella qingyii' 'Legionella sainthelensi' 15.9 [13.0 - 23.3 [21.0 - 16.0 [13.5 - 0.96 (QHJG00000000) (UFAM00000000) 19.4] 25.7] 18.9]

'Legionella qingyii' Legionella santicrucis 15.9 [12.9 - 23.3 [21.0 - 16.0 [13.5 - 1.15 (QHJG00000000) ATCC 35301 19.4] 25.7] 18.9]

'Legionella sp. km535' Legionella 13.1 [10.4 - 23.2 [20.9 - 13.5 [11.1 - 3.58 (RZGU00000000) birminghamensis ATCC 16.4] 25.6] 16.2] 43702

'Legionella qingyii' Legionella gratiana ATCC 16.6 [13.6 - 23.2 [21.0 - 16.7 [14.1 - 0.96 (QHJG00000000) 49413 20.2] 25.7] 19.6]

'Legionella sainthelensi' Legionella qingyii CCTCC 15.7 [12.8 - 23.2 [20.9 - 15.8 [13.3 - 1.02 (UFAL00000000) AB 2018025 19.2] 25.7] 18.7] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella cincinnatiensis 16.0 [13.0 - 23.2 [20.9 - 16.1 [13.5 - 1.03 (QHJG00000000) ATCC 43753 19.4] 25.6] 19.0]

'Legionella qingyii' 'Legionella sainthelensi' 15.7 [12.8 - 23.2 [20.9 - 15.8 [13.3 - 1.02 (QHJG00000000) (UFAL00000000) 19.2] 25.7] 18.7]

'Legionella qingyii' Legionella saoudiensis 14.3 [11.5 - 23.2 [20.9 - 14.6 [12.1 - 1.61 (RZGX00000000) LH-SWC 17.7] 25.7] 17.4]

'Legionella sainthelensi' Legionella steelei ATCC 16.2 [13.2 - 23.2 [20.9 - 16.2 [13.7 - 1.92 (UFAM00000000) BAA-2169 19.6] 25.7] 19.2]

'Legionella qingyii' 'Legionella sainthelensi' 16.2 [13.2 - 23.2 [20.9 - 16.3 [13.7 - 1.04 (RZGW00000000) (UFAL00000000) 19.7] 25.7] 19.2]

'Legionella qingyii' 'Legionella sainthelensi' 16.1 [13.1 - 23.1 [20.8 - 16.2 [13.6 - 1.04 (RZGX00000000) (UFAL00000000) 19.6] 25.5] 19.1]

'Legionella qingyii' Legionella beliardensis 12.7 [10.0 - 23.1 [20.8 - 13.1 [10.8 - 0.49 (RZGW00000000) NCTC 13315 16.0] 25.6] 15.9]

'Legionella sainthelensi' Legionella wadsworthii 15.4 [12.5 - 23.1 [20.8 - 15.6 [13.0 - 1.14 (UFAM00000000) ATCC 33877 18.8] 25.6] 18.4]

'Legionella qingyii' Legionella gratiana ATCC 17.2 [14.2 - 23.1 [20.8 - 17.2 [14.6 - 0.97 (RZGX00000000) 49413 20.8] 25.5] 20.2]

'Legionella qingyii' Legionella santicrucis 16.3 [13.3 - 23.1 [20.8 - 16.3 [13.8 - 1.17 (RZGX00000000) ATCC 35301 19.8] 25.6] 19.3]

'Legionella qingyii' Legionella gratiana ATCC 17.4 [14.3 - 23.1 [20.9 - 17.3 [14.7 - 0.98 (RZGW00000000) 49413 20.9] 25.6] 20.3]

'Legionella qingyii' Legionella cincinnatiensis 16.5 [13.5 - 23.1 [20.8 - 16.6 [14.0 - 1.04 (RZGX00000000) ATCC 43753 20.0] 25.5] 19.5]

'Legionella qingyii' Legionella santicrucis 16.3 [13.3 - 23.1 [20.8 - 16.3 [13.8 - 1.17 (RZGW00000000) ATCC 35301 19.8] 25.5] 19.3]

'Legionella sainthelensi' Legionella anisa ATCC 16.4 [13.5 - 23.0 [20.8 - 16.5 [13.9 - 1.41 (UFAL00000000) 35292 19.9] 25.5] 19.4]

'Legionella sainthelensi' Legionella brunensis 12.9 [10.2 - 23.0 [20.7 - 13.3 [10.9 - 1.88 (UFAL00000000) ATCC 43878 16.2] 25.4] 16.0]

'Legionella Legionella santicrucis 13.0 [10.3 - 23.0 [20.7 - 13.4 [11.0 - 5.66 septentrionalis' ATCC 35301 16.3] 25.4] 16.1] (RZGV00000000)

'Legionella sainthelensi' Legionella fallonii LLAP- 13.6 [10.8 - 22.9 [20.6 - 13.9 [11.5 - 1.48 (UFAM00000000) 10 17.0] 25.4] 16.7]

'Legionella sainthelensi' Legionella pneumophila 13.1 [10.4 - 22.9 [20.7 - 13.5 [11.1 - 1.05 (UFAL00000000) subsp. fraseri ATCC 16.4] 25.4] 16.2] 33156

'Legionella sainthelensi' Legionella parisiensis 16.2 [13.3 - 22.9 [20.6 - 16.3 [13.7 - 1.2 (UFAL00000000) DSM 19216 19.7] 25.3] 19.2]

'Legionella Legionella erythra ATCC 13.3 [10.6 - 22.9 [20.6 - 13.7 [11.3 - 4.49 septentrionalis' 35303 16.6] 25.3] 16.5] (RZGR00000000)

'Legionella qingyii' Legionella cincinnatiensis 16.6 [13.6 - 22.9 [20.6 - 16.6 [14.0 - 1.05 (RZGW00000000) ATCC 43753 20.1] 25.4] 19.5]

'Legionella sp. km772' Legionella 12.9 [10.2 - 22.9 [20.6 - 13.3 [10.9 - 4.48 (RZGQ00000000) birminghamensis ATCC 16.2] 25.4] 16.0] 43702

'Legionella qingyii' Legionella sainthelensi 16.3 [13.3 - 22.8 [20.6 - 16.3 [13.8 - 0.85 (RZGW00000000) ATCC 35248 19.7] 25.3] 19.2]

'Legionella sainthelensi' Legionella massiliensis 12.9 [10.2 - 22.8 [20.5 - 13.3 [11.0 - 4.41 (UFAL00000000) LegA 16.2] 25.3] 16.1]

'Legionella qingyii' Legionella dumoffii NY 23 21.4 [18.1 - 22.8 [20.5 - 20.6 [17.9 - 1.84 (QHJG00000000) 25.0] 25.2] 23.7]

'Legionella qingyii' Legionella dumoffii NY 23 23.2 [19.9 - 22.8 [20.5 - 22.1 [19.3 - 1.82 (RZGW00000000) 26.8] 25.3] 25.1] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella sainthelensi 16.1 [13.2 - 22.8 [20.5 - 16.2 [13.6 - 0.85 (RZGX00000000) ATCC 35248 19.6] 25.2] 19.1]

'Legionella sainthelensi' Legionella tucsonensis 16.5 [13.5 - 22.7 [20.4 - 16.5 [13.9 - 0.63 (UFAL00000000) ATCC 49180 20.0] 25.1] 19.4]

'Legionella sainthelensi' Legionella bozemanae 16.1 [13.1 - 22.7 [20.4 - 16.2 [13.6 - 1.13 (UFAL00000000) ATCC 33217 19.6] 25.2] 19.1]

'Legionella qingyii' Legionella dumoffii NY 23 23.1 [19.8 - 22.7 [20.4 - 22.0 [19.2 - 1.82 (RZGX00000000) 26.7] 25.2] 25.1]

'Legionella qingyii' Legionella sainthelensi 15.7 [12.8 - 22.7 [20.5 - 15.8 [13.3 - 0.83 (QHJG00000000) ATCC 35248 19.2] 25.2] 18.7]

'Legionella sp. km535' 'Legionella 13.1 [10.4 - 22.7 [20.4 - 13.5 [11.1 - 2.43 (RZGU00000000) septentrionalis' 16.4] 25.1] 16.2] (RZGV00000000)

'Legionella Legionella waltersii ATCC 13.1 [10.4 - 22.7 [20.4 - 13.5 [11.1 - 3.24 septentrionalis' 51914 16.4] 25.1] 16.2] (RZGV00000000)

'Legionella Legionella waltersii ATCC 13.2 [10.5 - 22.6 [20.3 - 13.5 [11.2 - 3.09 septentrionalis' 51914 16.5] 25.0] 16.3] (RZGS00000000)

'Legionella Legionella beliardensis 13.0 [10.3 - 22.6 [20.3 - 13.3 [11.0 - 4.83 septentrionalis' NCTC 13315 16.3] 25.0] 16.1] (RZGS00000000)

'Legionella sainthelensi' Legionella steelei ATCC 16.4 [13.4 - 22.6 [20.3 - 16.4 [13.8 - 1.99 (UFAL00000000) BAA-2169 19.8] 25.0] 19.3]

'Legionella qingyii' Legionella longbeachae 15.9 [13.0 - 22.6 [20.3 - 16.0 [13.4 - 0.82 (QHJG00000000) NCTC 11477 19.4] 25.0] 18.9]

'Legionella sainthelensi' Legionella steigerwaltii 16.3 [13.3 - 22.6 [20.4 - 16.4 [13.8 - 1.48 (UFAL00000000) ATCC 35302 19.8] 25.1] 19.3]

'Legionella qingyii' Legionella fallonii LLAP- 13.5 [10.8 - 22.6 [20.3 - 13.9 [11.5 - 0.52 (QHJG00000000) 10 16.9] 25.1] 16.7]

'Legionella sainthelensi' Legionella pneumophila 13.4 [10.6 - 22.6 [20.4 - 13.7 [11.3 - 1.49 (UFAL00000000) Philadelphia 1 16.7] 25.1] 16.5]

'Legionella sainthelensi' Legionella cherrii DSM 15.7 [12.8 - 22.5 [20.3 - 15.8 [13.3 - 2.02 (UFAM00000000) 19213 19.2] 25.0] 18.7]

'Legionella Legionella sainthelensi 12.9 [10.2 - 22.5 [20.3 - 13.3 [10.9 - 5.19 septentrionalis' ATCC 35248 16.2] 25.0] 16.0] (RZGS00000000)

'Legionella sp. km772' Legionella dumoffii NY 23 13.5 [10.7 - 22.5 [20.2 - 13.8 [11.4 - 0.66 (RZGQ00000000) 16.8] 25.0] 16.6]

'Legionella qingyii' Legionella fallonii LLAP- 13.7 [10.9 - 22.5 [20.3 - 14.0 [11.6 - 0.5 (RZGX00000000) 10 17.0] 25.0] 16.8]

'Legionella sainthelensi' Legionella pneumophila 13.3 [10.6 - 22.5 [20.2 - 13.7 [11.3 - 1.24 (UFAM00000000) subsp. pascullei ATCC 16.7] 24.9] 16.5] 33737

'Legionella sainthelensi' Legionella gormanii ATCC 16.5 [13.5 - 22.5 [20.2 - 16.5 [13.9 - 1.3 (UFAL00000000) 33342 20.0] 24.9] 19.4]

'Legionella sainthelensi' Legionella gormanii ATCC 16.5 [13.5 - 22.5 [20.2 - 16.5 [13.9 - 1.3 (UFAL00000000) 33297 20.0] 24.9] 19.4]

'Legionella sp. km535' Legionella worsleiensis 22.7 [19.4 - 22.5 [20.3 - 21.7 [18.9 - 0.43 (RZGU00000000) ATCC 49508 26.3] 25.0] 24.7]

'Legionella qingyii' 'Legionella sainthelensi' 16.1 [13.2 - 22.5 [20.3 - 16.2 [13.6 - 0.98 (RZGW00000000) (UFAM00000000) 19.6] 25.0] 19.1]

'Legionella qingyii' Legionella longbeachae 16.6 [13.6 - 22.5 [20.2 - 16.6 [14.0 - 0.83 (RZGW00000000) NCTC 11477 20.1] 24.9] 19.6]

'Legionella sainthelensi' Legionella tucsonensis 16.4 [13.4 - 22.5 [20.2 - 16.4 [13.8 - 0.57 (UFAM00000000) ATCC 49180 19.9] 24.9] 19.3] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella sp. km772' 'Legionella sainthelensi' 13.3 [10.5 - 22.5 [20.2 - 13.6 [11.3 - 2.14 (RZGQ00000000) (UFAM00000000) 16.6] 25.0] 16.4]

'Legionella sainthelensi' Legionella waltersii ATCC 13.4 [10.6 - 22.4 [20.1 - 13.7 [11.3 - 2.23 (UFAM00000000) 51914 16.7] 24.8] 16.5]

'Legionella qingyii' Legionella longbeachae 16.5 [13.5 - 22.4 [20.1 - 16.5 [13.9 - 0.83 (RZGX00000000) NCTC 11477 20.0] 24.8] 19.4]

'Legionella sp. km535' Legionella anisa ATCC 13.5 [10.7 - 22.4 [20.2 - 13.8 [11.4 - 1.7 (RZGU00000000) 35292 16.8] 24.9] 16.6]

'Legionella qingyii' Legionella beliardensis 12.7 [10.0 - 22.4 [20.1 - 13.1 [10.7 - 0.49 (RZGX00000000) NCTC 13315 16.0] 24.9] 15.8]

'Legionella Legionella pneumophila 13.2 [10.5 - 22.4 [20.2 - 13.6 [11.2 - 4.08 septentrionalis' subsp. pascullei ATCC 16.6] 24.9] 16.4] (RZGS00000000) 33737

'Legionella Legionella jordanis ATCC 13.1 [10.4 - 22.3 [20.1 - 13.5 [11.1 - 0.63 septentrionalis' 33623 16.4] 24.8] 16.3] (RZGV00000000)

'Legionella sp. km535' Legionella steelei ATCC 13.6 [10.9 - 22.3 [20.1 - 13.9 [11.5 - 1.12 (RZGU00000000) BAA-2169 17.0] 24.8] 16.7]

'Legionella sainthelensi' Legionella drancourtii 14.0 [11.2 - 22.3 [20.0 - 14.3 [11.9 - 2.46 (UFAL00000000) LLAP 12 17.4] 24.7] 17.1]

'Legionella sp. km772' Legionella sainthelensi 13.2 [10.5 - 22.3 [20.0 - 13.6 [11.2 - 2.01 (RZGQ00000000) ATCC 35248 16.5] 24.7] 16.3]

'Legionella Legionella clemsonensis 13.2 [10.5 - 22.3 [20.0 - 13.6 [11.2 - 2.84 septentrionalis' CDC-D5610 16.5] 24.7] 16.4] (RZGR00000000)

'Legionella sainthelensi' Legionella shakespearei 13.2 [10.5 - 22.3 [20.0 - 13.6 [11.2 - 4.86 (UFAM00000000) DSM 23087 16.6] 24.8] 16.4]

'Legionella qingyii' Legionella brunensis 12.9 [10.2 - 22.2 [19.9 - 13.2 [10.9 - 0.86 (QHJG00000000) ATCC 43878 16.1] 24.6] 16.0]

'Legionella sainthelensi' Legionella oakridgensis 13.0 [10.3 - 22.2 [19.9 - 13.4 [11.0 - 4.08 (UFAL00000000) ATCC 33761 16.3] 24.6] 16.1]

'Legionella sp. km535' Legionella rowbothamii 13.3 [10.5 - 22.1 [19.9 - 13.6 [11.2 - 0.56 (RZGU00000000) LLAP6 16.6] 24.6] 16.4]

'Legionella Legionella 13.0 [10.3 - 22.1 [19.9 - 13.4 [11.0 - 1.31 septentrionalis' birminghamensis ATCC 16.3] 24.6] 16.2] (RZGS00000000) 43702

'Legionella qingyii' Legionella saoudiensis 14.2 [11.3 - 22.1 [19.8 - 14.4 [12.0 - 1.6 (RZGW00000000) LH-SWC 17.5] 24.5] 17.3]

'Legionella sainthelensi' Legionella impletisoli 12.6 [9.9 - 22.1 [19.9 - 13.0 [10.7 - 3.33 (UFAM00000000) OA1-1 15.9] 24.6] 15.8]

'Legionella qingyii' Legionella wadsworthii 19.3 [16.1 - 22.0 [19.7 - 18.8 [16.1 - 0.16 (RZGX00000000) ATCC 33877 22.9] 24.4] 21.8]

'Legionella qingyii' Legionella wadsworthii 19.4 [16.3 - 22.0 [19.7 - 19.0 [16.3 - 0.16 (RZGW00000000) ATCC 33877 23.0] 24.5] 22.0]

'Legionella qingyii' Legionella drancourtii 14.3 [11.5 - 22.0 [19.7 - 14.6 [12.1 - 1.44 (QHJG00000000) LLAP 12 17.7] 24.4] 17.4]

'Legionella sp. km535' Legionella dumoffii NY 23 13.7 [10.9 - 22.0 [19.7 - 14.0 [11.6 - 0.25 (RZGU00000000) 17.0] 24.4] 16.8]

'Legionella sainthelensi' Legionella rowbothamii 13.8 [11.0 - 22.0 [19.7 - 14.1 [11.7 - 2.55 (UFAL00000000) LLAP6 17.1] 24.4] 16.9]

'Legionella qingyii' Legionella waltersii ATCC 13.3 [10.6 - 22.0 [19.7 - 13.6 [11.3 - 1.25 (RZGW00000000) 51914 16.6] 24.4] 16.4]

'Legionella qingyii' Legionella drancourtii 14.6 [11.7 - 22.0 [19.7 - 14.8 [12.3 - 1.42 (RZGW00000000) LLAP 12 18.0] 24.4] 17.7]

'Legionella Legionella fallonii LLAP- 12.6 [10.0 - 22.0 [19.8 - 13.0 [10.7 - 4.0 septentrionalis' 10 15.9] 24.5] 15.8] (RZGT00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella wadsworthii 18.2 [15.1 - 22.0 [19.7 - 18.0 [15.3 - 0.18 (QHJG00000000) ATCC 33877 21.8] 24.4] 20.9]

'Legionella sp. km535' 'Legionella sainthelensi' 13.5 [10.7 - 21.9 [19.6 - 13.8 [11.4 - 3.04 (RZGU00000000) (UFAM00000000) 16.8] 24.3] 16.6]

'Legionella qingyii' Legionella oakridgensis 13.0 [10.3 - 21.9 [19.7 - 13.4 [11.0 - 3.04 (RZGW00000000) ATCC 33761 16.3] 24.4] 16.1]

'Legionella sainthelensi' Legionella norrlandica 13.3 [10.6 - 21.9 [19.7 - 13.7 [11.3 - 0.63 (UFAM00000000) LEGN 16.7] 24.4] 16.5]

'Legionella sainthelensi' Legionella impletisoli 12.6 [9.9 - 21.9 [19.7 - 13.0 [10.7 - 3.32 (UFAM00000000) JCM 13919 15.9] 24.4] 15.8]

'Legionella qingyii' Legionella brunensis 12.9 [10.2 - 21.8 [19.6 - 13.3 [10.9 - 0.84 (RZGW00000000) ATCC 43878 16.2] 24.3] 16.1]

'Legionella qingyii' Legionella drancourtii 14.5 [11.7 - 21.8 [19.6 - 14.8 [12.3 - 1.42 (RZGX00000000) LLAP 12 17.9] 24.3] 17.6]

'Legionella qingyii' Legionella brunensis 12.9 [10.2 - 21.8 [19.6 - 13.3 [10.9 - 0.85 (RZGX00000000) ATCC 43878 16.2] 24.3] 16.1]

'Legionella qingyii' Legionella pneumophila 13.5 [10.8 - 21.8 [19.5 - 13.9 [11.5 - 0.45 (RZGW00000000) Philadelphia 1 16.9] 24.2] 16.7]

'Legionella sp. km535' Legionella parisiensis 13.6 [10.9 - 21.7 [19.5 - 13.9 [11.5 - 1.91 (RZGU00000000) DSM 19216 17.0] 24.2] 16.7]

'Legionella qingyii' Legionella pneumophila 13.4 [10.6 - 21.7 [19.4 - 13.7 [11.3 - 0.28 (QHJG00000000) subsp. pascullei ATCC 16.7] 24.1] 16.5] 33737

'Legionella sp. km535' Legionella cincinnatiensis 13.5 [10.8 - 21.7 [19.4 - 13.9 [11.5 - 3.11 (RZGU00000000) ATCC 43753 16.9] 24.1] 16.7]

'Legionella sp. km772' Legionella rowbothamii 13.3 [10.5 - 21.7 [19.4 - 13.6 [11.2 - 0.35 (RZGQ00000000) LLAP6 16.6] 24.1] 16.4]

'Legionella sainthelensi' Legionella drancourtii 14.0 [11.2 - 21.7 [19.5 - 14.3 [11.9 - 2.4 (UFAM00000000) LLAP 12 17.4] 24.1] 17.1]

'Legionella sp. km772' Legionella steigerwaltii 13.1 [10.4 - 21.7 [19.5 - 13.5 [11.1 - 0.71 (RZGQ00000000) ATCC 35302 16.4] 24.2] 16.2]

'Legionella sp. km772' Legionella fallonii LLAP- 13.6 [10.8 - 21.7 [19.4 - 13.9 [11.5 - 0.66 (RZGQ00000000) 10 16.9] 24.1] 16.7]

'Legionella sainthelensi' Legionella pneumophila 13.4 [10.7 - 21.7 [19.5 - 13.7 [11.3 - 1.3 (UFAL00000000) subsp. pascullei ATCC 16.7] 24.2] 16.5] 33737

'Legionella sainthelensi' Legionella hackeliae 13.0 [10.3 - 21.7 [19.5 - 13.3 [11.0 - 2.19 (UFAL00000000) ATCC 35250 16.3] 24.1] 16.1]

'Legionella sainthelensi' Legionella rowbothamii 13.8 [11.0 - 21.7 [19.5 - 14.1 [11.7 - 2.49 (UFAM00000000) LLAP6 17.1] 24.2] 16.9]

'Legionella qingyii' Legionella pneumophila 13.4 [10.6 - 21.6 [19.4 - 13.7 [11.3 - 0.47 (QHJG00000000) Philadelphia 1 16.7] 24.1] 16.5]

'Legionella Legionella brunensis 13.0 [10.3 - 21.6 [19.3 - 13.4 [11.0 - 3.65 septentrionalis' ATCC 43878 16.3] 24.0] 16.1] (RZGV00000000)

'Legionella qingyii' Legionella pneumophila 13.5 [10.8 - 21.6 [19.4 - 13.8 [11.4 - 0.26 (RZGX00000000) subsp. pascullei ATCC 16.9] 24.0] 16.6] 33737

'Legionella Legionella moravica DSM 13.1 [10.4 - 21.6 [19.3 - 13.5 [11.1 - 2.15 septentrionalis' 19234 16.4] 24.0] 16.2] (RZGS00000000)

'Legionella sainthelensi' Legionella fallonii LLAP- 13.4 [10.6 - 21.6 [19.3 - 13.7 [11.3 - 1.54 (UFAL00000000) 10 16.7] 24.0] 16.5]

'Legionella Legionella brunensis 13.0 [10.3 - 21.5 [19.2 - 13.4 [11.0 - 3.49 septentrionalis' ATCC 43878 16.3] 23.9] 16.1] (RZGS00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella waltersii ATCC 13.2 [10.5 - 21.5 [19.2 - 13.6 [11.2 - 1.27 (QHJG00000000) 51914 16.5] 23.9] 16.3]

'Legionella Legionella parisiensis 12.9 [10.2 - 21.5 [19.2 - 13.3 [10.9 - 4.23 septentrionalis' DSM 19216 16.2] 23.9] 16.0] (RZGR00000000)

'Legionella qingyii' Legionella fallonii LLAP- 13.5 [10.7 - 21.5 [19.3 - 13.8 [11.4 - 0.5 (RZGW00000000) 10 16.8] 24.0] 16.6]

'Legionella Legionella jordanis ATCC 13.2 [10.4 - 21.5 [19.2 - 13.5 [11.1 - 0.52 septentrionalis' 33623 16.5] 23.9] 16.3] (RZGR00000000)

'Legionella sp. km772' Legionella yabuuchiae 12.9 [10.2 - 21.5 [19.2 - 13.2 [10.9 - 1.99 (RZGQ00000000) OA1-2 16.1] 23.9] 16.0]

'Legionella sainthelensi' Legionella clemsonensis 13.1 [10.4 - 21.5 [19.3 - 13.4 [11.1 - 2.52 (UFAM00000000) CDC-D5610 16.4] 24.0] 16.2]

'Legionella Legionella 13.0 [10.3 - 21.5 [19.3 - 13.3 [11.0 - 1.15 septentrionalis' birminghamensis ATCC 16.3] 24.0] 16.1] (RZGV00000000) 43702

'Legionella sainthelensi' Legionella norrlandica 13.4 [10.7 - 21.5 [19.3 - 13.7 [11.4 - 0.69 (UFAL00000000) LEGN 16.7] 23.9] 16.5]

'Legionella Legionella massiliensis 13.0 [10.2 - 21.5 [19.3 - 13.3 [11.0 - 0.97 septentrionalis' LegA 16.2] 24.0] 16.1] (RZGS00000000)

'Legionella qingyii' Legionella pneumophila 13.5 [10.7 - 21.5 [19.2 - 13.8 [11.4 - 0.45 (RZGX00000000) Philadelphia 1 16.8] 23.9] 16.6]

'Legionella qingyii' Legionella pneumophila 13.5 [10.8 - 21.5 [19.3 - 13.8 [11.4 - 0.26 (RZGW00000000) subsp. pascullei ATCC 16.9] 24.0] 16.6] 33737

'Legionella qingyii' Legionella oakridgensis 12.9 [10.2 - 21.5 [19.3 - 13.2 [10.9 - 3.06 (QHJG00000000) ATCC 33761 16.1] 24.0] 16.0]

'Legionella sainthelensi' Legionella dumoffii NY 23 15.6 [12.6 - 21.4 [19.2 - 15.6 [13.1 - 2.86 (UFAL00000000) 19.0] 23.9] 18.5]

'Legionella qingyii' Legionella waltersii ATCC 13.3 [10.5 - 21.4 [19.1 - 13.6 [11.2 - 1.25 (RZGX00000000) 51914 16.6] 23.8] 16.4]

'Legionella qingyii' Legionella hackeliae 13.1 [10.4 - 21.4 [19.1 - 13.4 [11.1 - 1.14 (RZGW00000000) ATCC 35250 16.4] 23.8] 16.2]

'Legionella sainthelensi' Legionella beliardensis 12.7 [10.0 - 21.4 [19.2 - 13.1 [10.8 - 0.55 (UFAL00000000) NCTC 13315 16.0] 23.9] 15.9]

'Legionella Legionella moravica DSM 13.1 [10.4 - 21.4 [19.1 - 13.5 [11.1 - 2.31 septentrionalis' 19234 16.4] 23.8] 16.3] (RZGV00000000)

'Legionella sp. km535' Legionella 13.1 [10.4 - 21.4 [19.2 - 13.4 [11.1 - 0.47 (RZGU00000000) jamestowniensis DSM 16.4] 23.9] 16.2] 19215

'Legionella qingyii' Legionella moravica DSM 13.5 [10.7 - 21.4 [19.1 - 13.8 [11.4 - 2.18 (RZGW00000000) 19234 16.8] 23.8] 16.6]

'Legionella sainthelensi' Legionella impletisoli 12.6 [9.9 - 21.4 [19.2 - 13.0 [10.7 - 3.39 (UFAL00000000) OA1-1 15.9] 23.9] 15.8]

'Legionella sp. km772' Legionella 13.1 [10.4 - 21.4 [19.2 - 13.5 [11.1 - 0.43 (RZGQ00000000) jamestowniensis DSM 16.4] 23.8] 16.2] 19215

'Legionella Legionella hackeliae 12.9 [10.2 - 21.3 [19.0 - 13.3 [11.0 - 3.19 septentrionalis' ATCC 35250 16.2] 23.7] 16.1] (RZGS00000000)

'Legionella sp. km535' Legionella saoudiensis 13.4 [10.7 - 21.3 [19.1 - 13.8 [11.4 - 0.46 (RZGU00000000) LH-SWC 16.8] 23.8] 16.5]

'Legionella sp. km772' Legionella saoudiensis 13.3 [10.6 - 21.3 [19.1 - 13.7 [11.3 - 0.45 (RZGQ00000000) LH-SWC 16.7] 23.7] 16.5] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella 'Legionella sainthelensi' 12.9 [10.2 - 21.3 [19.1 - 13.3 [10.9 - 5.47 septentrionalis' (UFAM00000000) 16.2] 23.7] 16.0] (RZGV00000000)

'Legionella qingyii' Legionella clemsonensis 12.9 [10.2 - 21.2 [19.0 - 13.2 [10.9 - 1.56 (QHJG00000000) CDC-D5610 16.1] 23.7] 16.0]

'Legionella sainthelensi' Legionella saoudiensis 13.9 [11.1 - 21.2 [19.0 - 14.2 [11.8 - 2.65 (UFAL00000000) LH-SWC 17.3] 23.6] 17.0]

'Legionella sainthelensi' Legionella oakridgensis 13.0 [10.3 - 21.2 [19.0 - 13.4 [11.0 - 4.02 (UFAM00000000) ATCC 33761 16.3] 23.6] 16.2]

'Legionella qingyii' Legionella moravica DSM 13.3 [10.6 - 21.2 [19.0 - 13.6 [11.3 - 2.2 (QHJG00000000) 19234 16.6] 23.7] 16.4]

'Legionella Legionella shakespearei 12.9 [10.2 - 21.2 [18.9 - 13.3 [10.9 - 0.61 septentrionalis' DSM 23087 16.2] 23.6] 16.1] (RZGV00000000)

'Legionella sainthelensi' Legionella impletisoli 12.6 [9.9 - 21.2 [18.9 - 13.0 [10.7 - 3.38 (UFAL00000000) JCM 13919 15.9] 23.6] 15.8]

'Legionella sp. km535' Legionella santicrucis 13.5 [10.7 - 21.1 [18.8 - 13.8 [11.4 - 3.23 (RZGU00000000) ATCC 35301 16.8] 23.5] 16.6]

'Legionella Legionella pneumophila 12.8 [10.1 - 21.1 [18.8 - 13.2 [10.9 - 4.37 septentrionalis' subsp. fraseri ATCC 16.1] 23.5] 16.0] (RZGR00000000) 33156

'Legionella Legionella santicrucis 13.0 [10.3 - 21.1 [18.9 - 13.4 [11.0 - 5.51 septentrionalis' ATCC 35301 16.3] 23.6] 16.1] (RZGS00000000)

'Legionella sp. km535' Legionella clemsonensis 13.0 [10.3 - 21.1 [18.8 - 13.4 [11.0 - 0.52 (RZGU00000000) CDC-D5610 16.3] 23.5] 16.2]

'Legionella sainthelensi' Tamlana fucoidanivorans 12.5 [9.8 - 21.1 [18.8 - 12.9 [10.6 - 2.49 (UFAL00000000) CW2-9T 15.8] 23.5] 15.6]

'Legionella qingyii' Legionella moravica DSM 13.4 [10.7 - 21.1 [18.9 - 13.8 [11.4 - 2.18 (RZGX00000000) 19234 16.8] 23.6] 16.6]

'Legionella sp. km772' Legionella anisa ATCC 13.4 [10.7 - 21.0 [18.7 - 13.7 [11.4 - 0.79 (RZGQ00000000) 35292 16.8] 23.4] 16.5]

'Legionella qingyii' Legionella shakespearei 13.7 [10.9 - 21.0 [18.7 - 13.9 [11.5 - 3.88 (RZGW00000000) DSM 23087 17.0] 23.4] 16.8]

'Legionella qingyii' Legionella worsleiensis 13.5 [10.7 - 21.0 [18.7 - 13.8 [11.4 - 2.49 (RZGW00000000) ATCC 49508 16.8] 23.4] 16.6]

'Legionella Legionella 13.3 [10.5 - 21.0 [18.8 - 13.6 [11.2 - 2.9 septentrionalis' jamestowniensis DSM 16.6] 23.4] 16.4] (RZGV00000000) 19215

'Legionella Legionella pneumophila 12.8 [10.1 - 21.0 [18.8 - 13.2 [10.8 - 4.48 septentrionalis' subsp. fraseri ATCC 16.1] 23.4] 16.0] (RZGV00000000) 33156

'Legionella sp. km535' Legionella bozemanae 13.3 [10.6 - 21.0 [18.8 - 13.7 [11.3 - 1.98 (RZGU00000000) ATCC 33217 16.7] 23.4] 16.5]

'Legionella sp. km772' Legionella hackeliae 13.2 [10.4 - 21.0 [18.7 - 13.5 [11.1 - 0.01 (RZGQ00000000) ATCC 35250 16.5] 23.4] 16.3]

'Legionella Legionella pneumophila 12.8 [10.1 - 21.0 [18.7 - 13.2 [10.8 - 4.33 septentrionalis' subsp. fraseri ATCC 16.1] 23.4] 15.9] (RZGS00000000) 33156

'Legionella qingyii' Legionella clemsonensis 12.9 [10.2 - 21.0 [18.7 - 13.3 [10.9 - 1.54 (RZGX00000000) CDC-D5610 16.2] 23.4] 16.0]

'Legionella sp. km535' Legionella gratiana ATCC 13.6 [10.9 - 20.9 [18.7 - 13.9 [11.5 - 3.04 (RZGU00000000) 49413 17.0] 23.3] 16.7]

'Legionella Tamlana fucoidanivorans 12.5 [9.8 - 20.9 [18.6 - 12.9 [10.6 - 7.86 septentrionalis' CW2-9T 15.8] 23.3] 15.7] (RZGS00000000)

'Legionella qingyii' Legionella oakridgensis 13.0 [10.3 - 20.9 [18.7 - 13.3 [11.0 - 3.04 (RZGX00000000) ATCC 33761 16.2] 23.4] 16.1] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella anisa ATCC 12.9 [10.2 - 20.9 [18.7 - 13.3 [10.9 - 4.13 septentrionalis' 35292 16.2] 23.3] 16.1] (RZGV00000000)

'Legionella Legionella massiliensis 12.9 [10.2 - 20.9 [18.7 - 13.3 [10.9 - 1.12 septentrionalis' LegA 16.2] 23.3] 16.1] (RZGV00000000)

'Legionella qingyii' Legionella worsleiensis 13.4 [10.7 - 20.9 [18.6 - 13.7 [11.3 - 2.49 (RZGX00000000) ATCC 49508 16.7] 23.3] 16.5]

'Legionella qingyii' Legionella worsleiensis 13.3 [10.5 - 20.9 [18.7 - 13.6 [11.2 - 2.51 (QHJG00000000) ATCC 49508 16.6] 23.3] 16.4]

'Legionella qingyii' Legionella rowbothamii 14.0 [11.2 - 20.9 [18.7 - 14.3 [11.8 - 1.51 (RZGW00000000) LLAP6 17.4] 23.3] 17.1]

'Legionella sp. km535' Legionella steigerwaltii 13.4 [10.7 - 20.9 [18.7 - 13.8 [11.4 - 1.62 (RZGU00000000) ATCC 35302 16.8] 23.4] 16.6]

'Legionella qingyii' Legionella norrlandica 13.5 [10.8 - 20.9 [18.7 - 13.8 [11.4 - 0.35 (RZGW00000000) LEGN 16.9] 23.3] 16.6]

'Legionella Legionella dumoffii NY 23 13.0 [10.3 - 20.9 [18.7 - 13.4 [11.0 - 2.67 septentrionalis' 16.3] 23.3] 16.1] (RZGV00000000)

'Legionella qingyii' Legionella clemsonensis 13.0 [10.3 - 20.9 [18.7 - 13.4 [11.0 - 1.54 (RZGW00000000) CDC-D5610 16.3] 23.4] 16.1]

'Legionella sp. km772' Legionella jordanis ATCC 13.0 [10.3 - 20.9 [18.7 - 13.4 [11.0 - 2.7 (RZGQ00000000) 33623 16.3] 23.3] 16.2]

'Legionella qingyii' 'Legionella sp. km535' 13.5 [10.7 - 20.8 [18.6 - 13.8 [11.4 - 2.08 (QHJG00000000) (RZGU00000000) 16.8] 23.2] 16.6]

'Legionella sp. km772' Tamlana fucoidanivorans 12.5 [9.8 - 20.8 [18.6 - 12.9 [10.6 - 4.69 (RZGQ00000000) CW2-9T 15.8] 23.3] 15.6]

'Legionella qingyii' Legionella quateirensis 13.6 [10.8 - 20.8 [18.6 - 13.9 [11.5 - 1.2 (QHJG00000000) ATCC 49507 16.9] 23.3] 16.7]

'Legionella sp. km535' Legionella oakridgensis 12.8 [10.1 - 20.8 [18.6 - 13.2 [10.8 - 0.97 (RZGU00000000) ATCC 33761 16.0] 23.2] 15.9]

'Legionella sainthelensi' Legionella 12.9 [10.2 - 20.8 [18.6 - 13.2 [10.9 - 6.68 (UFAL00000000) birminghamensis ATCC 16.2] 23.2] 16.0] 43702

'Legionella sp. km535' 'Legionella qingyii' 13.7 [10.9 - 20.8 [18.6 - 14.0 [11.6 - 2.06 (RZGU00000000) (RZGW00000000) 17.0] 23.3] 16.8]

'Legionella qingyii' Legionella rowbothamii 13.9 [11.1 - 20.8 [18.6 - 14.2 [11.7 - 1.51 (RZGX00000000) LLAP6 17.3] 23.3] 17.0]

'Legionella sp. km535' Legionella qingyii CCTCC 13.5 [10.7 - 20.8 [18.6 - 13.8 [11.4 - 2.09 (RZGU00000000) AB 2018025 16.8] 23.2] 16.6]

'Legionella Tamlana fucoidanivorans 12.5 [9.8 - 20.8 [18.6 - 12.9 [10.6 - 7.91 septentrionalis' CW2-9T 15.8] 23.2] 15.6] (RZGR00000000)

'Legionella qingyii' Legionella norrlandica 13.4 [10.7 - 20.8 [18.5 - 13.7 [11.3 - 0.33 (QHJG00000000) LEGN 16.7] 23.2] 16.5]

'Legionella qingyii' Legionella rowbothamii 13.7 [10.9 - 20.8 [18.6 - 14.0 [11.6 - 1.53 (QHJG00000000) LLAP6 17.1] 23.3] 16.8]

'Legionella qingyii' Legionella massiliensis 13.0 [10.3 - 20.8 [18.5 - 13.4 [11.0 - 3.37 (RZGW00000000) LegA 16.3] 23.2] 16.1]

'Legionella Legionella hackeliae 12.9 [10.2 - 20.8 [18.5 - 13.3 [10.9 - 3.35 septentrionalis' ATCC 35250 16.2] 23.2] 16.0] (RZGV00000000)

'Legionella sp. km535' Legionella gormanii ATCC 13.7 [11.0 - 20.8 [18.6 - 14.0 [11.6 - 1.81 (RZGU00000000) 33297 17.1] 23.2] 16.8]

'Legionella qingyii' Legionella quateirensis 13.7 [11.0 - 20.8 [18.5 - 14.0 [11.6 - 1.18 (RZGX00000000) ATCC 49507 17.1] 23.2] 16.8] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella nagasakiensis 12.9 [10.2 - 20.8 [18.5 - 13.3 [10.9 - 3.24 (RZGW00000000) JCM 15315 16.2] 23.2] 16.1]

'Legionella Legionella hackeliae 12.9 [10.2 - 20.8 [18.6 - 13.3 [10.9 - 3.24 septentrionalis' ATCC 35250 16.2] 23.2] 16.1] (RZGR00000000)

'Legionella sainthelensi' Legionella nagasakiensis 13.1 [10.4 - 20.8 [18.6 - 13.4 [11.1 - 4.22 (UFAM00000000) JCM 15315 16.4] 23.2] 16.2]

'Legionella Legionella pneumophila 13.2 [10.4 - 20.8 [18.6 - 13.5 [11.1 - 3.93 septentrionalis' Philadelphia 1 16.5] 23.3] 16.3] (RZGR00000000)

'Legionella sainthelensi' Legionella beliardensis 12.8 [10.1 - 20.8 [18.6 - 13.2 [10.8 - 0.49 (UFAM00000000) NCTC 13315 16.1] 23.3] 15.9]

'Legionella qingyii' Legionella norrlandica 13.5 [10.8 - 20.7 [18.5 - 13.9 [11.5 - 0.35 (RZGX00000000) LEGN 16.9] 23.1] 16.7]

'Legionella sainthelensi' Legionella shakespearei 13.4 [10.6 - 20.7 [18.5 - 13.7 [11.3 - 4.92 (UFAL00000000) DSM 23087 16.7] 23.1] 16.5]

'Legionella sainthelensi' Legionella quateirensis 13.4 [10.7 - 20.7 [18.5 - 13.7 [11.3 - 2.22 (UFAL00000000) ATCC 49507 16.7] 23.2] 16.5]

'Legionella sp. km772' 'Legionella sainthelensi' 13.3 [10.5 - 20.7 [18.5 - 13.6 [11.2 - 2.2 (RZGQ00000000) (UFAL00000000) 16.6] 23.2] 16.4]

'Legionella Legionella gratiana ATCC 13.1 [10.3 - 20.7 [18.5 - 13.4 [11.1 - 5.36 septentrionalis' 49413 16.4] 23.1] 16.2] (RZGR00000000)

'Legionella Legionella fairfieldensis 13.2 [10.5 - 20.7 [18.5 - 13.6 [11.2 - 2.67 septentrionalis' ATCC 49588 16.5] 23.1] 16.3] (RZGV00000000)

'Legionella sp. km772' Legionella norrlandica 13.3 [10.5 - 20.7 [18.5 - 13.6 [11.2 - 1.51 (RZGQ00000000) LEGN 16.6] 23.2] 16.4]

'Legionella sp. km535' Legionella shakespearei 15.5 [12.6 - 20.7 [18.5 - 15.6 [13.1 - 1.81 (RZGU00000000) DSM 23087 19.0] 23.1] 18.5]

'Legionella sainthelensi' Legionella clemsonensis 13.0 [10.3 - 20.7 [18.5 - 13.4 [11.0 - 2.58 (UFAL00000000) CDC-D5610 16.3] 23.2] 16.2]

'Legionella sp. km535' 'Legionella qingyii' 13.6 [10.8 - 20.7 [18.5 - 13.9 [11.5 - 2.07 (RZGU00000000) (RZGX00000000) 16.9] 23.2] 16.7]

'Legionella sainthelensi' Legionella 12.9 [10.2 - 20.7 [18.4 - 13.3 [10.9 - 2.63 (UFAL00000000) jamestowniensis DSM 16.2] 23.1] 16.1] 19215

'Legionella Legionella norrlandica 13.1 [10.4 - 20.7 [18.5 - 13.5 [11.1 - 4.73 septentrionalis' LEGN 16.4] 23.1] 16.2] (RZGR00000000)

'Legionella sp. km535' Legionella tucsonensis 13.5 [10.7 - 20.7 [18.4 - 13.8 [11.4 - 2.48 (RZGU00000000) ATCC 49180 16.8] 23.1] 16.6]

'Legionella qingyii' Legionella quateirensis 13.8 [11.0 - 20.7 [18.5 - 14.1 [11.7 - 1.18 (RZGW00000000) ATCC 49507 17.1] 23.2] 16.9]

'Legionella sp. km535' Legionella gormanii ATCC 13.7 [11.0 - 20.7 [18.5 - 14.0 [11.6 - 1.8 (RZGU00000000) 33342 17.1] 23.1] 16.8]

'Legionella sp. km535' Legionella cherrii DSM 13.5 [10.7 - 20.6 [18.4 - 13.8 [11.4 - 1.03 (RZGU00000000) 19213 16.8] 23.0] 16.6]

'Legionella qingyii' Tamlana fucoidanivorans 12.5 [9.8 - 20.6 [18.4 - 12.9 [10.6 - 3.51 (QHJG00000000) CW2-9T 15.8] 23.0] 15.6]

'Legionella Legionella 13.1 [10.3 - 20.6 [18.4 - 13.4 [11.1 - 2.92 septentrionalis' jamestowniensis DSM 16.4] 23.0] 16.2] (RZGT00000000) 19215

'Legionella Legionella erythra ATCC 13.3 [10.5 - 20.6 [18.3 - 13.6 [11.2 - 4.38 septentrionalis' 35303 16.6] 23.0] 16.4] (RZGV00000000)

'Legionella sainthelensi' Legionella wadsworthii 15.0 [12.1 - 20.6 [18.4 - 15.2 [12.7 - 1.2 (UFAL00000000) ATCC 33877 18.5] 23.0] 18.0] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella moravica DSM 13.1 [10.4 - 20.6 [18.3 - 13.5 [11.1 - 2.2 septentrionalis' 19234 16.4] 23.0] 16.2] (RZGR00000000)

'Legionella Legionella fairfieldensis 13.2 [10.5 - 20.6 [18.3 - 13.5 [11.2 - 2.51 septentrionalis' ATCC 49588 16.5] 23.0] 16.3] (RZGS00000000)

'Legionella sp. km535' 'Legionella sainthelensi' 13.5 [10.7 - 20.6 [18.4 - 13.8 [11.4 - 3.11 (RZGU00000000) (UFAL00000000) 16.8] 23.0] 16.6]

'Legionella sainthelensi' Legionella massiliensis 12.9 [10.2 - 20.6 [18.4 - 13.2 [10.9 - 4.35 (UFAM00000000) LegA 16.2] 23.0] 16.0]

'Legionella sp. km772' Legionella longbeachae 13.1 [10.4 - 20.6 [18.4 - 13.5 [11.1 - 1.99 (RZGQ00000000) NCTC 11477 16.4] 23.0] 16.2]

'Legionella sp. km535' Legionella jordanis ATCC 12.9 [10.2 - 20.6 [18.4 - 13.3 [10.9 - 1.8 (RZGU00000000) 33623 16.2] 23.0] 16.1]

'Legionella qingyii' Legionella shakespearei 13.5 [10.7 - 20.6 [18.4 - 13.8 [11.4 - 3.9 (QHJG00000000) DSM 23087 16.8] 23.0] 16.6]

'Legionella qingyii' Legionella shakespearei 13.6 [10.8 - 20.6 [18.4 - 13.9 [11.5 - 3.88 (RZGX00000000) DSM 23087 16.9] 23.0] 16.7]

'Legionella sp. km535' Legionella longbeachae 13.4 [10.6 - 20.6 [18.4 - 13.7 [11.3 - 2.9 (RZGU00000000) NCTC 11477 16.7] 23.0] 16.5]

'Legionella Legionella cincinnatiensis 12.9 [10.2 - 20.5 [18.2 - 13.2 [10.9 - 5.54 septentrionalis' ATCC 43753 16.2] 22.9] 16.0] (RZGV00000000)

'Legionella sp. km535' Legionella wadsworthii 13.4 [10.6 - 20.5 [18.3 - 13.7 [11.3 - 1.91 (RZGU00000000) ATCC 33877 16.7] 23.0] 16.5]

'Legionella sainthelensi' Legionella moravica DSM 13.4 [10.6 - 20.5 [18.2 - 13.7 [11.3 - 3.22 (UFAL00000000) 19234 16.7] 22.9] 16.5]

'Legionella Legionella quateirensis 12.9 [10.2 - 20.5 [18.3 - 13.3 [10.9 - 3.31 septentrionalis' ATCC 49507 16.2] 22.9] 16.0] (RZGV00000000)

'Legionella sainthelensi' Legionella waltersii ATCC 13.4 [10.6 - 20.5 [18.3 - 13.7 [11.3 - 2.29 (UFAL00000000) 51914 16.7] 23.0] 16.5]

'Legionella sp. km535' Legionella impletisoli 12.7 [10.1 - 20.5 [18.3 - 13.1 [10.8 - 0.28 (RZGU00000000) OA1-1 16.0] 22.9] 15.9]

'Legionella sp. km535' Tamlana fucoidanivorans 12.5 [9.8 - 20.5 [18.2 - 12.9 [10.6 - 5.59 (RZGU00000000) CW2-9T 15.8] 22.9] 15.6]

'Legionella Legionella impletisoli 13.3 [10.5 - 20.5 [18.2 - 13.6 [11.2 - 2.14 septentrionalis' OA1-1 16.6] 22.9] 16.4] (RZGV00000000)

'Legionella sp. km772' Legionella quateirensis 13.8 [11.0 - 20.5 [18.3 - 14.0 [11.6 - 0.02 (RZGQ00000000) ATCC 49507 17.1] 22.9] 16.9]

'Legionella sp. km535' Legionella drancourtii 13.7 [10.9 - 20.5 [18.3 - 14.0 [11.5 - 0.64 (RZGU00000000) LLAP 12 17.0] 22.9] 16.8]

'Legionella sainthelensi' Legionella nagasakiensis 13.1 [10.4 - 20.5 [18.3 - 13.4 [11.1 - 4.28 (UFAL00000000) JCM 15315 16.4] 22.9] 16.2]

'Legionella Legionella impletisoli 13.2 [10.5 - 20.4 [18.2 - 13.6 [11.2 - 2.16 septentrionalis' JCM 13919 16.6] 22.8] 16.4] (RZGV00000000)

'Legionella sainthelensi' Legionella rubrilucens 12.9 [10.2 - 20.4 [18.2 - 13.3 [10.9 - 10.88 (UFAL00000000) ATCC 35304 16.2] 22.8] 16.0]

'Legionella sp. km535' Legionella impletisoli 12.7 [10.1 - 20.4 [18.1 - 13.1 [10.8 - 0.27 (RZGU00000000) JCM 13919 16.0] 22.8] 15.9]

'Legionella sp. km772' Legionella cincinnatiensis 13.2 [10.5 - 20.4 [18.2 - 13.5 [11.2 - 2.2 (RZGQ00000000) ATCC 43753 16.5] 22.8] 16.3]

'Legionella sainthelensi' Tamlana fucoidanivorans 12.5 [9.8 - 20.4 [18.2 - 12.9 [10.6 - 2.55 (UFAM00000000) CW2-9T 15.8] 22.8] 15.6] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella 12.9 [10.2 - 20.4 [18.2 - 13.3 [10.9 - 1.59 (RZGW00000000) jamestowniensis DSM 16.2] 22.8] 16.0] 19215

'Legionella sp. km772' Legionella clemsonensis 12.9 [10.2 - 20.3 [18.1 - 13.3 [10.9 - 0.38 (RZGQ00000000) CDC-D5610 16.2] 22.7] 16.1]

'Legionella sp. km535' Legionella beliardensis 12.8 [10.1 - 20.3 [18.1 - 13.2 [10.8 - 2.55 (RZGU00000000) NCTC 13315 16.1] 22.7] 15.9]

'Legionella sainthelensi' Legionella erythra ATCC 12.8 [10.1 - 20.3 [18.0 - 13.2 [10.8 - 9.91 (UFAL00000000) 35303 16.1] 22.7] 15.9]

'Legionella sp. km772' Legionella worsleiensis 13.6 [10.9 - 20.3 [18.0 - 13.9 [11.5 - 1.33 (RZGQ00000000) ATCC 49508 17.0] 22.7] 16.7]

'Legionella sp. km772' Legionella septentrionalis 12.9 [10.2 - 20.3 [18.1 - 13.3 [10.9 - 3.15 (RZGQ00000000) km711T 16.2] 22.7] 16.1]

'Legionella sp. km772' Legionella gratiana ATCC 13.4 [10.6 - 20.3 [18.1 - 13.7 [11.3 - 2.13 (RZGQ00000000) 49413 16.7] 22.7] 16.5]

'Legionella sp. km772' Legionella parisiensis 13.3 [10.6 - 20.3 [18.1 - 13.6 [11.3 - 1.0 (RZGQ00000000) DSM 19216 16.6] 22.7] 16.4]

'Legionella sp. km772' Legionella brunensis 13.0 [10.3 - 20.3 [18.1 - 13.3 [11.0 - 0.31 (RZGQ00000000) ATCC 43878 16.3] 22.7] 16.1]

'Legionella sainthelensi' Legionella moravica DSM 13.4 [10.6 - 20.3 [18.1 - 13.7 [11.3 - 3.16 (UFAM00000000) 19234 16.7] 22.7] 16.5]

'Legionella sainthelensi' Legionella rubrilucens 12.9 [10.2 - 20.3 [18.1 - 13.2 [10.9 - 10.81 (UFAM00000000) ATCC 35304 16.1] 22.7] 16.0]

'Legionella sp. km772' 'Legionella 12.9 [10.2 - 20.3 [18.1 - 13.2 [10.9 - 3.35 (RZGQ00000000) septentrionalis' 16.2] 22.7] 16.0] (RZGT00000000)

'Legionella sp. km535' Legionella waltersii ATCC 13.9 [11.1 - 20.3 [18.1 - 14.2 [11.8 - 0.82 (RZGU00000000) 51914 17.3] 22.7] 17.0]

'Legionella Legionella massiliensis 12.9 [10.2 - 20.3 [18.1 - 13.3 [10.9 - 1.01 septentrionalis' LegA 16.2] 22.7] 16.1] (RZGR00000000)

'Legionella qingyii' Tamlana fucoidanivorans 12.5 [9.8 - 20.3 [18.1 - 12.9 [10.6 - 3.53 (RZGW00000000) CW2-9T 15.8] 22.7] 15.6]

'Legionella sp. km772' 'Legionella 12.9 [10.2 - 20.3 [18.1 - 13.3 [10.9 - 3.18 (RZGQ00000000) septentrionalis' 16.2] 22.7] 16.1] (RZGS00000000)

'Legionella sainthelensi' Legionella yabuuchiae 12.7 [10.0 - 20.3 [18.1 - 13.1 [10.7 - 4.13 (UFAM00000000) OA1-2 16.0] 22.7] 15.8]

'Legionella Legionella tucsonensis 12.8 [10.1 - 20.2 [18.0 - 13.2 [10.8 - 4.75 septentrionalis' ATCC 49180 16.1] 22.6] 16.0] (RZGS00000000)

'Legionella qingyii' Legionella nagasakiensis 12.9 [10.2 - 20.2 [18.0 - 13.2 [10.9 - 3.26 (QHJG00000000) JCM 15315 16.2] 22.6] 16.0]

'Legionella Legionella 12.9 [10.2 - 20.2 [17.9 - 13.3 [10.9 - 1.26 septentrionalis' birminghamensis ATCC 16.2] 22.6] 16.0] (RZGR00000000) 43702

'Legionella Legionella quateirensis 12.9 [10.2 - 20.2 [18.0 - 13.3 [10.9 - 3.2 septentrionalis' ATCC 49507 16.2] 22.6] 16.1] (RZGR00000000)

'Legionella sp. km772' Legionella wadsworthii 13.2 [10.4 - 20.2 [17.9 - 13.5 [11.1 - 1.0 (RZGQ00000000) ATCC 33877 16.5] 22.6] 16.3]

'Legionella sainthelensi' Legionella fairfieldensis 13.0 [10.3 - 20.2 [17.9 - 13.3 [11.0 - 2.86 (UFAL00000000) ATCC 49588 16.3] 22.6] 16.1]

'Legionella Legionella brunensis 12.9 [10.2 - 20.2 [18.0 - 13.3 [10.9 - 3.66 septentrionalis' ATCC 43878 16.2] 22.6] 16.1] (RZGT00000000)

'Legionella qingyii' Legionella rubrilucens 12.8 [10.1 - 20.2 [18.0 - 13.2 [10.8 - 9.83 (RZGW00000000) ATCC 35304 16.1] 22.7] 16.0] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella massiliensis 12.9 [10.2 - 20.2 [17.9 - 13.3 [10.9 - 3.39 (QHJG00000000) LegA 16.2] 22.6] 16.0]

'Legionella 'Legionella sainthelensi' 12.9 [10.2 - 20.2 [18.0 - 13.2 [10.9 - 5.42 septentrionalis' (UFAL00000000) 16.2] 22.6] 16.0] (RZGR00000000)

'Legionella sp. km772' Legionella bozemanae 13.3 [10.5 - 20.1 [17.9 - 13.6 [11.2 - 1.07 (RZGQ00000000) ATCC 33217 16.6] 22.6] 16.4]

'Legionella sp. km772' 'Legionella sp. km535' 13.7 [10.9 - 20.1 [17.9 - 14.0 [11.6 - 0.91 (RZGQ00000000) (RZGU00000000) 17.0] 22.5] 16.8]

'Legionella sp. km772' 'Legionella 12.8 [10.1 - 20.1 [17.9 - 13.2 [10.8 - 3.22 (RZGQ00000000) septentrionalis' 16.1] 22.5] 15.9] (RZGR00000000)

'Legionella qingyii' Legionella nagasakiensis 12.9 [10.2 - 20.1 [17.9 - 13.3 [10.9 - 3.24 (RZGX00000000) JCM 15315 16.2] 22.6] 16.0]

'Legionella sp. km535' Legionella hackeliae 13.0 [10.3 - 20.1 [17.9 - 13.4 [11.0 - 0.92 (RZGU00000000) ATCC 35250 16.3] 22.5] 16.1]

'Legionella sp. km772' Legionella oakridgensis 13.0 [10.3 - 20.1 [17.9 - 13.3 [11.0 - 1.88 (RZGQ00000000) ATCC 33761 16.3] 22.5] 16.1]

'Legionella sp. km772' Legionella cherrii DSM 13.2 [10.5 - 20.1 [17.9 - 13.5 [11.2 - 0.12 (RZGQ00000000) 19213 16.5] 22.5] 16.3]

'Legionella Legionella beliardensis 12.8 [10.1 - 20.1 [17.9 - 13.2 [10.8 - 4.98 septentrionalis' NCTC 13315 16.1] 22.5] 15.9] (RZGV00000000)

'Legionella Tamlana fucoidanivorans 12.5 [9.8 - 20.1 [17.9 - 12.9 [10.6 - 8.02 septentrionalis' CW2-9T 15.8] 22.5] 15.7] (RZGV00000000)

'Legionella Legionella anisa ATCC 12.9 [10.2 - 20.1 [17.9 - 13.3 [11.0 - 4.01 septentrionalis' 35292 16.2] 22.5] 16.1] (RZGR00000000)

'Legionella Legionella rubrilucens 13.3 [10.5 - 20.1 [17.9 - 13.6 [11.2 - 5.34 septentrionalis' ATCC 35304 16.6] 22.5] 16.4] (RZGV00000000)

'Legionella sp. km535' Legionella massiliensis 13.0 [10.3 - 20.1 [17.9 - 13.4 [11.0 - 1.31 (RZGU00000000) LegA 16.3] 22.5] 16.2]

'Legionella sp. km535' Legionella fallonii LLAP- 14.2 [11.4 - 20.1 [17.9 - 14.4 [12.0 - 1.56 (RZGU00000000) 10 17.5] 22.5] 17.2]

'Legionella qingyii' Legionella 12.9 [10.2 - 20.1 [17.9 - 13.2 [10.9 - 1.61 (QHJG00000000) jamestowniensis DSM 16.1] 22.5] 16.0] 19215

'Legionella sp. km535' Legionella norrlandica 13.9 [11.1 - 20.1 [17.9 - 14.2 [11.7 - 2.42 (RZGU00000000) LEGN 17.3] 22.5] 17.0]

'Legionella qingyii' Legionella massiliensis 13.0 [10.3 - 20.0 [17.8 - 13.3 [11.0 - 3.37 (RZGX00000000) LegA 16.3] 22.4] 16.1]

'Legionella Legionella anisa ATCC 12.9 [10.2 - 20.0 [17.8 - 13.3 [11.0 - 3.97 septentrionalis' 35292 16.2] 22.4] 16.1] (RZGS00000000)

'Legionella sp. km772' Legionella shakespearei 13.7 [10.9 - 20.0 [17.7 - 14.0 [11.6 - 2.72 (RZGQ00000000) DSM 23087 17.1] 22.4] 16.8]

'Legionella Legionella clemsonensis 12.9 [10.2 - 20.0 [17.8 - 13.3 [10.9 - 2.97 septentrionalis' CDC-D5610 16.2] 22.4] 16.0] (RZGT00000000)

'Legionella sp. km772' Legionella pneumophila 13.3 [10.6 - 20.0 [17.8 - 13.6 [11.3 - 1.15 (RZGQ00000000) subsp. fraseri ATCC 16.6] 22.4] 16.4] 33156

'Legionella sp. km772' Legionella waltersii ATCC 13.4 [10.6 - 20.0 [17.8 - 13.7 [11.3 - 0.09 (RZGQ00000000) 51914 16.7] 22.4] 16.5]

'Legionella Legionella pneumophila 13.2 [10.5 - 20.0 [17.7 - 13.6 [11.2 - 4.12 septentrionalis' subsp. pascullei ATCC 16.6] 22.4] 16.4] (RZGR00000000) 33737 Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella cincinnatiensis 12.9 [10.2 - 19.9 [17.7 - 13.3 [10.9 - 5.38 septentrionalis' ATCC 43753 16.2] 22.3] 16.0] (RZGS00000000)

'Legionella sp. km772' Legionella nagasakiensis 12.9 [10.2 - 19.9 [17.7 - 13.3 [10.9 - 2.08 (RZGQ00000000) JCM 15315 16.2] 22.3] 16.1]

'Legionella Legionella cincinnatiensis 13.0 [10.3 - 19.9 [17.7 - 13.3 [11.0 - 5.43 septentrionalis' ATCC 43753 16.2] 22.3] 16.1] (RZGR00000000)

'Legionella sp. km772' Legionella gormanii ATCC 13.3 [10.6 - 19.9 [17.7 - 13.6 [11.3 - 0.9 (RZGQ00000000) 33297 16.6] 22.3] 16.4]

'Legionella Legionella 13.2 [10.5 - 19.9 [17.7 - 13.6 [11.2 - 2.75 septentrionalis' jamestowniensis DSM 16.5] 22.3] 16.3] (RZGS00000000) 19215

'Legionella sainthelensi' Legionella worsleiensis 13.3 [10.6 - 19.9 [17.7 - 13.6 [11.2 - 3.47 (UFAM00000000) ATCC 49508 16.6] 22.3] 16.4]

'Legionella Tamlana fucoidanivorans 12.5 [9.8 - 19.8 [17.6 - 12.9 [10.6 - 8.04 septentrionalis' CW2-9T 15.8] 22.2] 15.6] (RZGT00000000)

'Legionella Legionella longbeachae 12.8 [10.1 - 19.8 [17.6 - 13.2 [10.8 - 5.33 septentrionalis' NCTC 11477 16.1] 22.2] 15.9] (RZGV00000000)

'Legionella sp. km772' Legionella gormanii ATCC 13.3 [10.6 - 19.8 [17.6 - 13.6 [11.3 - 0.9 (RZGQ00000000) 33342 16.6] 22.2] 16.4]

'Legionella Legionella tucsonensis 12.9 [10.2 - 19.8 [17.6 - 13.3 [10.9 - 4.9 septentrionalis' ATCC 49180 16.2] 22.2] 16.0] (RZGV00000000)

'Legionella Legionella erythra ATCC 13.2 [10.5 - 19.8 [17.6 - 13.5 [11.2 - 4.53 septentrionalis' 35303 16.5] 22.2] 16.3] (RZGS00000000)

'Legionella 'Legionella sainthelensi' 12.8 [10.1 - 19.8 [17.6 - 13.2 [10.8 - 5.36 septentrionalis' (UFAM00000000) 16.1] 22.2] 15.9] (RZGR00000000)

'Legionella Legionella tucsonensis 12.9 [10.2 - 19.8 [17.6 - 13.3 [10.9 - 4.79 septentrionalis' ATCC 49180 16.2] 22.2] 16.1] (RZGR00000000)

'Legionella sainthelensi' Legionella fairfieldensis 13.0 [10.2 - 19.8 [17.6 - 13.3 [11.0 - 2.8 (UFAM00000000) ATCC 49588 16.2] 22.2] 16.1]

'Legionella sainthelensi' Legionella taurinensis 12.8 [10.1 - 19.8 [17.6 - 13.2 [10.9 - 11.21 (UFAM00000000) NCTC 13314 16.1] 22.2] 16.0]

'Legionella qingyii' Legionella taurinensis 12.9 [10.2 - 19.8 [17.6 - 13.3 [10.9 - 10.23 (RZGW00000000) NCTC 13314 16.2] 22.2] 16.0]

'Legionella sainthelensi' Legionella yabuuchiae 12.7 [10.0 - 19.8 [17.6 - 13.1 [10.7 - 4.19 (UFAL00000000) OA1-2 15.9] 22.2] 15.8]

'Legionella qingyii' 'Legionella sp. km772' 13.3 [10.6 - 19.7 [17.5 - 13.6 [11.2 - 1.18 (QHJG00000000) (RZGQ00000000) 16.6] 22.1] 16.4]

'Legionella sp. km772' 'Legionella 12.8 [10.1 - 19.7 [17.5 - 13.2 [10.8 - 3.33 (RZGQ00000000) septentrionalis' 16.1] 22.1] 15.9] (RZGV00000000)

'Legionella Legionella taurinensis 13.2 [10.5 - 19.7 [17.5 - 13.5 [11.2 - 5.74 septentrionalis' NCTC 13314 16.5] 22.1] 16.3] (RZGV00000000)

'Legionella Legionella massiliensis 12.9 [10.2 - 19.7 [17.5 - 13.2 [10.9 - 1.14 septentrionalis' LegA 16.1] 22.1] 16.0] (RZGT00000000)

'Legionella qingyii' Legionella 12.9 [10.2 - 19.7 [17.5 - 13.3 [10.9 - 1.59 (RZGX00000000) jamestowniensis DSM 16.2] 22.1] 16.0] 19215

'Legionella sp. km535' Legionella pneumophila 14.0 [11.2 - 19.7 [17.5 - 14.3 [11.8 - 2.05 (RZGU00000000) subsp. fraseri ATCC 17.4] 22.1] 17.1] 33156 Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella sp. km772' Legionella qingyii CCTCC 13.3 [10.6 - 19.7 [17.5 - 13.6 [11.2 - 1.18 (RZGQ00000000) AB 2018025 16.6] 22.1] 16.4]

'Legionella Legionella santicrucis 12.7 [10.0 - 19.7 [17.5 - 13.1 [10.7 - 5.68 septentrionalis' ATCC 35301 16.0] 22.1] 15.8] (RZGT00000000)

'Legionella qingyii' Legionella rubrilucens 12.7 [10.0 - 19.7 [17.5 - 13.1 [10.8 - 9.85 (QHJG00000000) ATCC 35304 16.0] 22.1] 15.9]

'Legionella sp. km535' Legionella septentrionalis 13.0 [10.3 - 19.6 [17.4 - 13.4 [11.0 - 2.24 (RZGU00000000) km711T 16.3] 22.0] 16.1]

'Legionella 'Legionella sp. km535' 12.9 [10.2 - 19.6 [17.4 - 13.3 [10.9 - 2.32 septentrionalis' (RZGU00000000) 16.2] 22.0] 16.1] (RZGR00000000)

'Legionella Legionella londiniensis 13.2 [10.5 - 19.6 [17.4 - 13.5 [11.2 - 1.14 septentrionalis' ATCC 49505 16.5] 22.0] 16.3] (RZGS00000000)

'Legionella 'Legionella sp. km535' 13.0 [10.3 - 19.6 [17.4 - 13.4 [11.0 - 2.27 septentrionalis' (RZGU00000000) 16.3] 22.0] 16.1] (RZGS00000000)

'Legionella Legionella gormanii ATCC 12.9 [10.2 - 19.6 [17.4 - 13.3 [10.9 - 4.24 septentrionalis' 33297 16.2] 22.0] 16.1] (RZGV00000000)

'Legionella sp. km772' 'Legionella qingyii' 13.4 [10.6 - 19.6 [17.4 - 13.7 [11.3 - 1.16 (RZGQ00000000) (RZGX00000000) 16.7] 22.0] 16.5]

'Legionella sp. km772' 'Legionella qingyii' 13.4 [10.6 - 19.6 [17.4 - 13.7 [11.3 - 1.16 (RZGQ00000000) (RZGW00000000) 16.7] 22.0] 16.5]

'Legionella qingyii' Legionella taurinensis 12.8 [10.1 - 19.6 [17.4 - 13.2 [10.8 - 10.25 (QHJG00000000) NCTC 13314 16.1] 22.0] 15.9]

'Legionella sp. km535' Legionella taurinensis 12.9 [10.2 - 19.6 [17.4 - 13.2 [10.9 - 8.17 (RZGU00000000) NCTC 13314 16.1] 22.0] 16.0]

'Legionella sainthelensi' Legionella worsleiensis 13.3 [10.6 - 19.6 [17.4 - 13.6 [11.2 - 3.53 (UFAL00000000) ATCC 49508 16.6] 22.0] 16.4]

'Legionella Legionella rubrilucens 13.2 [10.5 - 19.5 [17.3 - 13.5 [11.2 - 5.5 septentrionalis' ATCC 35304 16.5] 21.8] 16.3] (RZGS00000000)

'Legionella qingyii' Legionella jordanis ATCC 13.0 [10.3 - 19.5 [17.3 - 13.3 [11.0 - 3.86 (RZGW00000000) 33623 16.3] 21.9] 16.1]

'Legionella sp. km772' Legionella pneumophila 13.5 [10.8 - 19.5 [17.3 - 13.8 [11.4 - 0.71 (RZGQ00000000) Philadelphia 1 16.9] 21.9] 16.6]

'Legionella Legionella oakridgensis 13.5 [10.7 - 19.5 [17.3 - 13.8 [11.4 - 1.45 septentrionalis' ATCC 33761 16.8] 21.9] 16.6] (RZGV00000000)

'Legionella qingyii' Legionella fairfieldensis 12.9 [10.2 - 19.5 [17.3 - 13.3 [10.9 - 1.82 (RZGW00000000) ATCC 49588 16.2] 21.9] 16.0]

'Legionella sainthelensi' Legionella taurinensis 12.9 [10.2 - 19.5 [17.3 - 13.3 [10.9 - 11.27 (UFAL00000000) NCTC 13314 16.2] 21.9] 16.1]

'Legionella qingyii' Legionella jordanis ATCC 12.9 [10.2 - 19.5 [17.3 - 13.3 [10.9 - 3.88 (QHJG00000000) 33623 16.2] 21.9] 16.0]

'Legionella qingyii' Legionella rubrilucens 12.8 [10.1 - 19.5 [17.3 - 13.2 [10.8 - 9.84 (RZGX00000000) ATCC 35304 16.1] 21.9] 15.9]

'Legionella sp. km772' Legionella beliardensis 12.9 [10.2 - 19.5 [17.3 - 13.3 [10.9 - 1.65 (RZGQ00000000) NCTC 13315 16.2] 21.8] 16.0]

'Legionella Legionella steelei ATCC 12.9 [10.2 - 19.5 [17.3 - 13.3 [10.9 - 3.55 septentrionalis' BAA-2169 16.2] 21.9] 16.0] (RZGV00000000)

'Legionella Legionella hackeliae 12.9 [10.2 - 19.4 [17.2 - 13.2 [10.9 - 3.36 septentrionalis' ATCC 35250 16.2] 21.8] 16.0] (RZGT00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella sainthelensi' Legionella spiritensis 12.8 [10.1 - 19.4 [17.2 - 13.2 [10.9 - 8.54 (UFAM00000000) ATCC 35249 16.1] 21.8] 16.0]

'Legionella sp. km772' Legionella moravica DSM 13.6 [10.8 - 19.4 [17.2 - 13.8 [11.5 - 1.02 (RZGQ00000000) 19234 16.9] 21.8] 16.7]

'Legionella qingyii' Tamlana fucoidanivorans 12.5 [9.8 - 19.4 [17.2 - 12.9 [10.6 - 3.53 (RZGX00000000) CW2-9T 15.8] 21.8] 15.6]

'Legionella qingyii' Legionella fairfieldensis 12.8 [10.1 - 19.4 [17.2 - 13.2 [10.8 - 1.84 (QHJG00000000) ATCC 49588 16.1] 21.8] 16.0]

'Legionella Legionella steigerwaltii 12.8 [10.1 - 19.4 [17.2 - 13.2 [10.8 - 4.05 septentrionalis' ATCC 35302 16.1] 21.8] 15.9] (RZGV00000000)

'Legionella Legionella bozemanae 12.8 [10.1 - 19.4 [17.2 - 13.2 [10.8 - 4.25 septentrionalis' ATCC 33217 16.1] 21.8] 15.9] (RZGS00000000)

'Legionella sp. km535' Legionella londiniensis 12.7 [10.0 - 19.4 [17.2 - 13.1 [10.8 - 3.41 (RZGU00000000) ATCC 49505 16.0] 21.8] 15.9]

'Legionella sainthelensi' Legionella spiritensis 13.0 [10.3 - 19.4 [17.2 - 13.4 [11.0 - 8.6 (UFAL00000000) ATCC 35249 16.3] 21.8] 16.1]

'Legionella Legionella gormanii ATCC 12.9 [10.2 - 19.4 [17.2 - 13.3 [10.9 - 4.23 septentrionalis' 33342 16.2] 21.8] 16.1] (RZGV00000000)

'Legionella sainthelensi' Legionella jordanis ATCC 13.1 [10.4 - 19.4 [17.2 - 13.4 [11.1 - 4.84 (UFAM00000000) 33623 16.4] 21.8] 16.2]

'Legionella Legionella oakridgensis 13.5 [10.7 - 19.4 [17.2 - 13.8 [11.4 - 1.3 septentrionalis' ATCC 33761 16.8] 21.8] 16.6] (RZGS00000000)

'Legionella Legionella beliardensis 13.0 [10.3 - 19.4 [17.2 - 13.3 [11.0 - 4.87 septentrionalis' NCTC 13315 16.3] 21.8] 16.1] (RZGR00000000)

'Legionella sainthelensi' Legionella jordanis ATCC 13.1 [10.4 - 19.3 [17.1 - 13.5 [11.1 - 4.9 (UFAL00000000) 33623 16.4] 21.7] 16.2]

'Legionella qingyii' Legionella jordanis ATCC 12.9 [10.2 - 19.3 [17.1 - 13.3 [10.9 - 3.86 (RZGX00000000) 33623 16.2] 21.7] 16.0]

'Legionella sp. km535' Legionella pneumophila 14.6 [11.7 - 19.3 [17.1 - 14.7 [12.3 - 1.81 (RZGU00000000) subsp. pascullei ATCC 18.0] 21.7] 17.6] 33737

'Legionella sp. km772' Legionella tucsonensis 13.3 [10.5 - 19.3 [17.1 - 13.6 [11.2 - 1.57 (RZGQ00000000) ATCC 49180 16.6] 21.7] 16.4]

'Legionella sp. km535' Legionella rubrilucens 12.9 [10.2 - 19.3 [17.1 - 13.2 [10.9 - 7.77 (RZGU00000000) ATCC 35304 16.2] 21.7] 16.0]

'Legionella sp. km535' Legionella brunensis 13.1 [10.4 - 19.3 [17.1 - 13.4 [11.1 - 1.22 (RZGU00000000) ATCC 43878 16.4] 21.7] 16.2]

'Legionella Legionella oakridgensis 13.5 [10.8 - 19.3 [17.1 - 13.8 [11.4 - 1.47 septentrionalis' ATCC 33761 16.9] 21.7] 16.6] (RZGT00000000)

'Legionella qingyii' Legionella yabuuchiae 12.7 [10.0 - 19.3 [17.1 - 13.1 [10.7 - 3.15 (RZGW00000000) OA1-2 15.9] 21.7] 15.8]

'Legionella qingyii' Legionella yabuuchiae 12.6 [10.0 - 19.3 [17.1 - 13.0 [10.7 - 3.17 (QHJG00000000) OA1-2 15.9] 21.7] 15.8]

'Legionella Legionella worsleiensis 13.0 [10.3 - 19.3 [17.1 - 13.4 [11.0 - 2.0 septentrionalis' ATCC 49508 16.3] 21.7] 16.2] (RZGV00000000)

'Legionella sp. km772' Legionella fairfieldensis 12.9 [10.2 - 19.3 [17.1 - 13.2 [10.9 - 0.67 (RZGQ00000000) ATCC 49588 16.2] 21.7] 16.0]

'Legionella Legionella oakridgensis 13.6 [10.8 - 19.3 [17.1 - 13.8 [11.5 - 1.34 septentrionalis' ATCC 33761 16.9] 21.7] 16.7] (RZGR00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella worsleiensis 13.0 [10.3 - 19.2 [17.0 - 13.4 [11.0 - 1.85 septentrionalis' ATCC 49508 16.3] 21.6] 16.2] (RZGS00000000)

'Legionella sainthelensi' Legionella septentrionalis 12.9 [10.2 - 19.2 [17.0 - 13.3 [10.9 - 5.29 (UFAM00000000) km711T 16.2] 21.6] 16.0]

'Legionella Legionella impletisoli 13.1 [10.4 - 19.2 [17.0 - 13.5 [11.1 - 1.99 septentrionalis' OA1-1 16.5] 21.6] 16.3] (RZGS00000000)

'Legionella Legionella shakespearei 12.8 [10.1 - 19.2 [17.0 - 13.2 [10.8 - 0.63 septentrionalis' DSM 23087 16.1] 21.6] 15.9] (RZGT00000000)

'Legionella 'Legionella sainthelensi' 12.9 [10.2 - 19.2 [17.0 - 13.3 [10.9 - 5.32 septentrionalis' (UFAM00000000) 16.2] 21.6] 16.0] (RZGS00000000)

'Legionella Legionella sainthelensi 12.7 [10.0 - 19.2 [17.0 - 13.1 [10.8 - 5.36 septentrionalis' ATCC 35248 16.0] 21.6] 15.8] (RZGT00000000)

'Legionella Legionella saoudiensis 13.1 [10.3 - 19.2 [17.0 - 13.4 [11.0 - 2.89 septentrionalis' LH-SWC 16.4] 21.6] 16.2] (RZGV00000000)

'Legionella sp. km535' Legionella pneumophila 14.6 [11.8 - 19.2 [17.1 - 14.8 [12.3 - 1.61 (RZGU00000000) Philadelphia 1 18.0] 21.6] 17.6]

'Legionella Legionella nagasakiensis 13.2 [10.5 - 19.2 [17.0 - 13.6 [11.2 - 1.14 septentrionalis' JCM 15315 16.5] 21.6] 16.3] (RZGR00000000)

'Legionella qingyii' Legionella fairfieldensis 12.9 [10.2 - 19.2 [17.0 - 13.2 [10.9 - 1.83 (RZGX00000000) ATCC 49588 16.2] 21.5] 16.0]

'Legionella qingyii' Legionella taurinensis 12.8 [10.1 - 19.1 [16.9 - 13.2 [10.8 - 10.23 (RZGX00000000) NCTC 13314 16.1] 21.5] 15.9]

'Legionella Legionella quateirensis 12.9 [10.2 - 19.1 [17.0 - 13.3 [10.9 - 3.16 septentrionalis' ATCC 49507 16.2] 21.5] 16.1] (RZGS00000000)

'Legionella sp. km772' Legionella pneumophila 13.5 [10.7 - 19.1 [16.9 - 13.8 [11.4 - 0.9 (RZGQ00000000) subsp. pascullei ATCC 16.8] 21.5] 16.6] 33737

'Legionella Legionella pneumophila 12.8 [10.1 - 19.1 [16.9 - 13.1 [10.8 - 4.5 septentrionalis' subsp. fraseri ATCC 16.0] 21.5] 15.9] (RZGT00000000) 33156

'Legionella qingyii' Legionella erythra ATCC 12.9 [10.2 - 19.1 [16.9 - 13.3 [10.9 - 8.87 (RZGW00000000) 35303 16.2] 21.5] 16.0]

'Legionella sainthelensi' Legionella londiniensis 12.7 [10.0 - 19.1 [16.9 - 13.1 [10.7 - 6.52 (UFAL00000000) ATCC 49505 15.9] 21.5] 15.8]

'Legionella Legionella dumoffii NY 23 13.0 [10.3 - 19.1 [16.9 - 13.4 [11.0 - 2.52 septentrionalis' 16.3] 21.5] 16.1] (RZGS00000000)

'Legionella sp. km772' Legionella impletisoli 12.8 [10.1 - 19.1 [16.9 - 13.1 [10.8 - 1.19 (RZGQ00000000) OA1-1 16.0] 21.5] 15.9]

'Legionella Legionella nagasakiensis 13.3 [10.6 - 19.1 [16.9 - 13.6 [11.3 - 1.25 septentrionalis' JCM 15315 16.7] 21.5] 16.4] (RZGV00000000)

'Legionella Legionella shakespearei 12.8 [10.1 - 19.1 [16.9 - 13.2 [10.9 - 0.5 septentrionalis' DSM 23087 16.1] 21.5] 16.0] (RZGR00000000)

'Legionella 'Legionella sainthelensi' 12.9 [10.2 - 19.1 [16.9 - 13.3 [10.9 - 5.53 septentrionalis' (UFAL00000000) 16.2] 21.5] 16.0] (RZGV00000000)

'Legionella qingyii' Legionella impletisoli 12.8 [10.1 - 19.0 [16.8 - 13.1 [10.8 - 2.35 (RZGW00000000) OA1-1 16.0] 21.4] 15.9]

'Legionella Legionella taurinensis 13.2 [10.5 - 19.0 [16.8 - 13.5 [11.2 - 5.85 septentrionalis' NCTC 13314 16.5] 21.4] 16.3] (RZGR00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella steigerwaltii 12.8 [10.1 - 19.0 [16.8 - 13.2 [10.8 - 3.89 septentrionalis' ATCC 35302 16.1] 21.4] 15.9] (RZGS00000000)

'Legionella Legionella worsleiensis 13.0 [10.3 - 19.0 [16.9 - 13.4 [11.0 - 1.89 septentrionalis' ATCC 49508 16.3] 21.4] 16.2] (RZGR00000000)

'Legionella Legionella impletisoli 13.1 [10.4 - 19.0 [16.9 - 13.5 [11.1 - 2.0 septentrionalis' JCM 13919 16.4] 21.4] 16.3] (RZGS00000000)

'Legionella Legionella longbeachae 12.8 [10.1 - 19.0 [16.8 - 13.2 [10.8 - 5.21 septentrionalis' NCTC 11477 16.1] 21.4] 15.9] (RZGR00000000)

'Legionella Legionella spiritensis 13.1 [10.3 - 19.0 [16.8 - 13.4 [11.0 - 3.23 septentrionalis' ATCC 35249 16.4] 21.3] 16.2] (RZGS00000000)

'Legionella Legionella bozemanae 12.8 [10.1 - 19.0 [16.8 - 13.2 [10.8 - 4.29 septentrionalis' ATCC 33217 16.1] 21.4] 15.9] (RZGR00000000)

'Legionella Legionella shakespearei 12.9 [10.2 - 19.0 [16.8 - 13.3 [10.9 - 0.46 septentrionalis' DSM 23087 16.2] 21.4] 16.1] (RZGS00000000)

'Legionella sp. km772' Legionella impletisoli 12.8 [10.1 - 19.0 [16.8 - 13.1 [10.8 - 1.18 (RZGQ00000000) JCM 13919 16.0] 21.4] 15.9]

'Legionella sp. km535' Legionella yabuuchiae 12.8 [10.1 - 19.0 [16.8 - 13.1 [10.8 - 1.09 (RZGU00000000) OA1-2 16.0] 21.3] 15.9]

'Legionella qingyii' Legionella impletisoli 12.7 [10.0 - 18.9 [16.7 - 13.1 [10.7 - 2.37 (QHJG00000000) OA1-1 15.9] 21.2] 15.8]

'Legionella qingyii' Legionella 13.0 [10.3 - 18.9 [16.7 - 13.4 [11.0 - 5.64 (RZGW00000000) birminghamensis ATCC 16.3] 21.3] 16.1] 43702

'Legionella Legionella parisiensis 12.8 [10.1 - 18.9 [16.7 - 13.2 [10.8 - 4.35 septentrionalis' DSM 19216 16.1] 21.2] 15.9] (RZGT00000000)

'Legionella qingyii' Legionella impletisoli 12.8 [10.1 - 18.9 [16.7 - 13.1 [10.8 - 2.34 (RZGW00000000) JCM 13919 16.0] 21.3] 15.9]

'Legionella sp. km772' Legionella taurinensis 13.0 [10.2 - 18.9 [16.8 - 13.3 [11.0 - 9.07 (RZGQ00000000) NCTC 13314 16.2] 21.3] 16.1]

'Legionella Legionella wadsworthii 12.8 [10.1 - 18.9 [16.8 - 13.2 [10.8 - 4.18 septentrionalis' ATCC 33877 16.1] 21.3] 15.9] (RZGS00000000)

'Legionella qingyii' Legionella yabuuchiae 12.7 [10.0 - 18.9 [16.7 - 13.0 [10.7 - 3.15 (RZGX00000000) OA1-2 15.9] 21.3] 15.8]

'Legionella Legionella drancourtii 12.9 [10.2 - 18.9 [16.7 - 13.3 [10.9 - 2.91 septentrionalis' LLAP 12 16.2] 21.2] 16.0] (RZGS00000000)

'Legionella Legionella steigerwaltii 12.8 [10.1 - 18.9 [16.7 - 13.2 [10.8 - 3.94 septentrionalis' ATCC 35302 16.1] 21.3] 15.9] (RZGR00000000)

'Legionella Legionella drancourtii 12.9 [10.2 - 18.9 [16.7 - 13.3 [10.9 - 3.07 septentrionalis' LLAP 12 16.2] 21.3] 16.1] (RZGV00000000)

'Legionella Legionella impletisoli 13.1 [10.4 - 18.9 [16.7 - 13.5 [11.1 - 2.03 septentrionalis' OA1-1 16.4] 21.3] 16.2] (RZGR00000000)

'Legionella qingyii' Legionella spiritensis 12.9 [10.2 - 18.8 [16.6 - 13.3 [10.9 - 7.56 (RZGW00000000) ATCC 35249 16.2] 21.1] 16.0]

'Legionella qingyii' Legionella spiritensis 12.8 [10.1 - 18.8 [16.6 - 13.2 [10.8 - 7.58 (QHJG00000000) ATCC 35249 16.1] 21.1] 15.9]

'Legionella 'Legionella sp. km535' 12.9 [10.2 - 18.8 [16.6 - 13.3 [10.9 - 2.44 septentrionalis' (RZGU00000000) 16.2] 21.1] 16.0] (RZGT00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella qingyii' Legionella 12.9 [10.2 - 18.8 [16.6 - 13.3 [10.9 - 5.66 (QHJG00000000) birminghamensis ATCC 16.2] 21.2] 16.1] 43702

'Legionella Legionella beliardensis 12.8 [10.1 - 18.8 [16.6 - 13.1 [10.8 - 5.0 septentrionalis' NCTC 13315 16.0] 21.1] 15.9] (RZGT00000000)

'Legionella Legionella taurinensis 13.1 [10.4 - 18.8 [16.6 - 13.5 [11.1 - 5.9 septentrionalis' NCTC 13314 16.5] 21.2] 16.3] (RZGS00000000)

'Legionella Legionella impletisoli 13.1 [10.4 - 18.8 [16.6 - 13.4 [11.1 - 2.04 septentrionalis' JCM 13919 16.4] 21.2] 16.2] (RZGR00000000)

'Legionella sainthelensi' Legionella septentrionalis 13.0 [10.3 - 18.7 [16.6 - 13.4 [11.0 - 5.35 (UFAL00000000) km711T 16.3] 21.1] 16.2]

'Legionella 'Legionella sainthelensi' 13.0 [10.3 - 18.7 [16.6 - 13.4 [11.0 - 5.38 septentrionalis' (UFAL00000000) 16.3] 21.1] 16.2] (RZGS00000000)

'Legionella qingyii' Legionella spiritensis 12.8 [10.1 - 18.7 [16.6 - 13.2 [10.9 - 7.56 (RZGX00000000) ATCC 35249 16.1] 21.1] 16.0]

'Legionella qingyii' Legionella impletisoli 12.7 [10.0 - 18.7 [16.5 - 13.1 [10.7 - 2.36 (QHJG00000000) JCM 13919 15.9] 21.1] 15.8]

'Legionella Legionella moravica DSM 12.9 [10.2 - 18.7 [16.6 - 13.3 [10.9 - 2.33 septentrionalis' 19234 16.2] 21.1] 16.1] (RZGT00000000)

'Legionella Legionella taurinensis 13.2 [10.4 - 18.6 [16.4 - 13.5 [11.1 - 5.73 septentrionalis' NCTC 13314 16.5] 21.0] 16.3] (RZGT00000000)

'Legionella Legionella longbeachae 12.8 [10.1 - 18.6 [16.4 - 13.1 [10.8 - 5.17 septentrionalis' NCTC 11477 16.0] 20.9] 15.9] (RZGS00000000)

'Legionella Legionella fairfieldensis 13.0 [10.3 - 18.6 [16.5 - 13.3 [11.0 - 2.68 septentrionalis' ATCC 49588 16.2] 21.0] 16.1] (RZGT00000000)

'Legionella qingyii' Legionella erythra ATCC 12.9 [10.2 - 18.6 [16.4 - 13.3 [10.9 - 8.87 (RZGX00000000) 35303 16.2] 21.0] 16.0]

'Legionella sainthelensi' Legionella londiniensis 12.7 [10.0 - 18.6 [16.4 - 13.1 [10.8 - 6.46 (UFAM00000000) ATCC 49505 16.0] 20.9] 15.9]

'Legionella Legionella bozemanae 12.8 [10.1 - 18.6 [16.4 - 13.1 [10.8 - 4.4 septentrionalis' ATCC 33217 16.0] 20.9] 15.9] (RZGV00000000)

'Legionella qingyii' Legionella erythra ATCC 12.8 [10.1 - 18.6 [16.5 - 13.2 [10.9 - 8.89 (QHJG00000000) 35303 16.1] 21.0] 16.0]

'Legionella sp. km772' Legionella massiliensis 13.0 [10.3 - 18.6 [16.4 - 13.4 [11.0 - 2.21 (RZGQ00000000) LegA 16.3] 21.0] 16.1]

'Legionella Legionella nagasakiensis 13.2 [10.5 - 18.6 [16.4 - 13.5 [11.2 - 1.27 septentrionalis' JCM 15315 16.5] 21.0] 16.3] (RZGT00000000)

'Legionella Legionella 12.8 [10.1 - 18.5 [16.3 - 13.2 [10.8 - 1.14 septentrionalis' birminghamensis ATCC 16.1] 20.9] 15.9] (RZGT00000000) 43702

'Legionella sp. km772' Legionella erythra ATCC 13.0 [10.3 - 18.5 [16.4 - 13.3 [11.0 - 7.71 (RZGQ00000000) 35303 16.3] 20.9] 16.1]

'Legionella qingyii' Legionella 13.0 [10.3 - 18.5 [16.3 - 13.3 [11.0 - 5.64 (RZGX00000000) birminghamensis ATCC 16.3] 20.8] 16.1] 43702

'Legionella Legionella saoudiensis 13.1 [10.3 - 18.5 [16.3 - 13.4 [11.0 - 2.73 septentrionalis' LH-SWC 16.4] 20.9] 16.2] (RZGS00000000)

'Legionella Legionella steelei ATCC 12.9 [10.2 - 18.5 [16.4 - 13.3 [10.9 - 3.39 septentrionalis' BAA-2169 16.2] 20.9] 16.1] (RZGS00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella sp. km535' Legionella nagasakiensis 13.0 [10.2 - 18.5 [16.4 - 13.3 [10.9 - 1.18 (RZGU00000000) JCM 15315 16.2] 20.9] 16.1]

'Legionella Legionella jordanis ATCC 12.9 [10.2 - 18.5 [16.4 - 13.2 [10.9 - 0.65 septentrionalis' 33623 16.2] 20.9] 16.0] (RZGT00000000)

'Legionella qingyii' Legionella impletisoli 12.7 [10.0 - 18.5 [16.3 - 13.1 [10.8 - 2.35 (RZGX00000000) OA1-1 16.0] 20.9] 15.9]

'Legionella Legionella drancourtii 12.9 [10.2 - 18.4 [16.3 - 13.2 [10.9 - 2.96 septentrionalis' LLAP 12 16.2] 20.8] 16.0] (RZGR00000000)

'Legionella 'Legionella sainthelensi' 12.7 [10.0 - 18.4 [16.2 - 13.1 [10.7 - 5.49 septentrionalis' (UFAM00000000) 16.0] 20.8] 15.8] (RZGT00000000)

'Legionella Legionella steelei ATCC 12.8 [10.1 - 18.4 [16.3 - 13.2 [10.8 - 3.44 septentrionalis' BAA-2169 16.1] 20.8] 15.9] (RZGR00000000)

'Legionella Legionella wadsworthii 12.8 [10.1 - 18.4 [16.3 - 13.2 [10.8 - 4.33 septentrionalis' ATCC 33877 16.1] 20.8] 15.9] (RZGV00000000)

'Legionella sp. km535' Legionella spiritensis 12.9 [10.2 - 18.4 [16.3 - 13.3 [10.9 - 5.5 (RZGU00000000) ATCC 35249 16.2] 20.8] 16.0]

'Legionella 'Legionella sainthelensi' 12.8 [10.1 - 18.4 [16.3 - 13.1 [10.8 - 5.55 septentrionalis' (UFAL00000000) 16.0] 20.8] 15.9] (RZGT00000000)

'Legionella Legionella londiniensis 13.2 [10.5 - 18.4 [16.3 - 13.5 [11.1 - 1.1 septentrionalis' ATCC 49505 16.5] 20.8] 16.3] (RZGR00000000)

'Legionella Legionella londiniensis 13.2 [10.5 - 18.4 [16.3 - 13.5 [11.1 - 0.99 septentrionalis' ATCC 49505 16.5] 20.8] 16.3] (RZGV00000000)

'Legionella qingyii' Legionella londiniensis 12.7 [10.0 - 18.3 [16.2 - 13.1 [10.8 - 5.48 (RZGW00000000) ATCC 49505 16.0] 20.7] 15.9]

'Legionella sp. km772' Legionella rubrilucens 12.9 [10.2 - 18.3 [16.1 - 13.3 [10.9 - 8.68 (RZGQ00000000) ATCC 35304 16.2] 20.7] 16.1]

'Legionella Legionella rowbothamii 12.9 [10.2 - 18.3 [16.1 - 13.3 [10.9 - 2.87 septentrionalis' LLAP6 16.2] 20.6] 16.0] (RZGR00000000)

'Legionella qingyii' Legionella impletisoli 12.7 [10.0 - 18.3 [16.2 - 13.1 [10.8 - 2.34 (RZGX00000000) JCM 13919 16.0] 20.7] 15.8]

'Legionella qingyii' Legionella londiniensis 12.7 [10.0 - 18.3 [16.1 - 13.0 [10.7 - 5.5 (QHJG00000000) ATCC 49505 15.9] 20.6] 15.8]

'Legionella Legionella pneumophila 12.9 [10.2 - 18.2 [16.0 - 13.3 [10.9 - 4.06 septentrionalis' Philadelphia 1 16.2] 20.6] 16.0] (RZGT00000000)

'Legionella 'Legionella qingyii' 12.8 [10.1 - 18.2 [16.0 - 13.2 [10.9 - 4.38 septentrionalis' (RZGW00000000) 16.1] 20.6] 16.0] (RZGR00000000)

'Legionella Legionella wadsworthii 12.8 [10.1 - 18.2 [16.0 - 13.2 [10.8 - 4.22 septentrionalis' ATCC 33877 16.1] 20.5] 15.9] (RZGR00000000)

'Legionella Legionella saoudiensis 12.9 [10.2 - 18.2 [16.1 - 13.3 [10.9 - 2.78 septentrionalis' LH-SWC 16.2] 20.6] 16.1] (RZGR00000000)

'Legionella Legionella gormanii ATCC 12.9 [10.2 - 18.2 [16.0 - 13.2 [10.9 - 4.08 septentrionalis' 33297 16.1] 20.6] 16.0] (RZGS00000000)

'Legionella Legionella quateirensis 12.8 [10.1 - 18.2 [16.1 - 13.2 [10.8 - 3.33 septentrionalis' ATCC 49507 16.1] 20.6] 15.9] (RZGT00000000)

'Legionella sp. km535' Legionella fairfieldensis 13.1 [10.4 - 18.1 [16.0 - 13.4 [11.1 - 0.24 (RZGU00000000) ATCC 49588 16.4] 20.5] 16.2] Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella steigerwaltii 12.8 [10.1 - 18.1 [16.0 - 13.1 [10.8 - 4.06 septentrionalis' ATCC 35302 16.0] 20.5] 15.9] (RZGT00000000)

'Legionella Legionella rowbothamii 12.9 [10.2 - 18.1 [15.9 - 13.3 [10.9 - 2.98 septentrionalis' LLAP6 16.2] 20.4] 16.0] (RZGV00000000)

'Legionella Legionella waltersii ATCC 12.8 [10.1 - 18.1 [16.0 - 13.2 [10.8 - 3.26 septentrionalis' 51914 16.1] 20.5] 15.9] (RZGT00000000)

'Legionella Legionella dumoffii NY 23 12.8 [10.1 - 18.1 [15.9 - 13.2 [10.9 - 2.69 septentrionalis' 16.1] 20.4] 16.0] (RZGT00000000)

'Legionella Legionella steelei ATCC 12.8 [10.1 - 18.1 [15.9 - 13.2 [10.8 - 3.56 septentrionalis' BAA-2169 16.1] 20.4] 15.9] (RZGT00000000)

'Legionella sp. km772' Legionella londiniensis 12.8 [10.1 - 18.0 [15.8 - 13.2 [10.8 - 4.32 (RZGQ00000000) ATCC 49505 16.1] 20.3] 15.9]

'Legionella Legionella gormanii ATCC 12.8 [10.2 - 18.0 [15.9 - 13.2 [10.9 - 4.13 septentrionalis' 33297 16.1] 20.4] 16.0] (RZGR00000000)

'Legionella Legionella gratiana ATCC 12.8 [10.1 - 18.0 [15.8 - 13.1 [10.8 - 5.48 septentrionalis' 49413 16.0] 20.3] 15.9] (RZGT00000000)

'Legionella Legionella qingyii CCTCC 12.8 [10.1 - 18.0 [15.9 - 13.1 [10.8 - 4.4 septentrionalis' AB 2018025 16.0] 20.4] 15.9] (RZGR00000000)

'Legionella qingyii' 'Legionella 12.8 [10.1 - 18.0 [15.9 - 13.1 [10.8 - 4.4 (QHJG00000000) septentrionalis' 16.0] 20.4] 15.9] (RZGR00000000)

'Legionella sp. km535' Legionella erythra ATCC 13.0 [10.2 - 18.0 [15.8 - 13.3 [10.9 - 6.8 (RZGU00000000) 35303 16.2] 20.3] 16.1]

'Legionella 'Legionella qingyii' 13.0 [10.3 - 18.0 [15.9 - 13.3 [11.0 - 4.34 septentrionalis' (RZGW00000000) 16.3] 20.4] 16.1] (RZGS00000000)

'Legionella Legionella impletisoli 13.1 [10.4 - 18.0 [15.9 - 13.4 [11.1 - 2.16 septentrionalis' OA1-1 16.4] 20.3] 16.2] (RZGT00000000)

'Legionella Legionella anisa ATCC 12.7 [10.0 - 18.0 [15.9 - 13.1 [10.7 - 4.14 septentrionalis' 35292 16.0] 20.4] 15.8] (RZGT00000000)

'Legionella Legionella cherrii DSM 12.9 [10.2 - 18.0 [15.9 - 13.2 [10.9 - 3.3 septentrionalis' 19213 16.1] 20.4] 16.0] (RZGS00000000)

'Legionella Legionella spiritensis 12.8 [10.1 - 18.0 [15.9 - 13.2 [10.8 - 3.07 septentrionalis' ATCC 35249 16.1] 20.4] 15.9] (RZGV00000000)

'Legionella Legionella bozemanae 12.8 [10.1 - 18.0 [15.9 - 13.1 [10.8 - 4.42 septentrionalis' ATCC 33217 16.0] 20.4] 15.9] (RZGT00000000)

'Legionella Legionella norrlandica 12.8 [10.2 - 18.0 [15.9 - 13.2 [10.9 - 4.86 septentrionalis' LEGN 16.1] 20.4] 16.0] (RZGT00000000)

'Legionella 'Legionella qingyii' 13.0 [10.3 - 18.0 [15.9 - 13.3 [11.0 - 4.49 septentrionalis' (RZGW00000000) 16.2] 20.4] 16.1] (RZGV00000000)

'Legionella qingyii' Legionella septentrionalis 13.0 [10.3 - 18.0 [15.9 - 13.3 [11.0 - 4.31 (RZGW00000000) km711T 16.3] 20.4] 16.1]

'Legionella Legionella yabuuchiae 13.2 [10.4 - 17.9 [15.8 - 13.5 [11.1 - 1.23 septentrionalis' OA1-2 16.5] 20.2] 16.3] (RZGR00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella gormanii ATCC 12.8 [10.2 - 17.9 [15.8 - 13.2 [10.9 - 4.08 septentrionalis' 33342 16.1] 20.3] 16.0] (RZGS00000000)

'Legionella Legionella cherrii DSM 12.9 [10.2 - 17.9 [15.8 - 13.2 [10.9 - 3.34 septentrionalis' 19213 16.2] 20.3] 16.0] (RZGR00000000)

'Legionella Legionella cherrii DSM 12.9 [10.2 - 17.9 [15.8 - 13.3 [10.9 - 3.46 septentrionalis' 19213 16.2] 20.3] 16.0] (RZGV00000000)

'Legionella Legionella yabuuchiae 13.2 [10.4 - 17.9 [15.7 - 13.5 [11.1 - 1.34 septentrionalis' OA1-2 16.5] 20.2] 16.3] (RZGV00000000)

'Legionella Legionella londiniensis 13.1 [10.4 - 17.9 [15.8 - 13.4 [11.1 - 0.97 septentrionalis' ATCC 49505 16.4] 20.3] 16.2] (RZGT00000000)

'Legionella Legionella impletisoli 13.1 [10.4 - 17.9 [15.8 - 13.4 [11.0 - 2.17 septentrionalis' JCM 13919 16.4] 20.3] 16.2] (RZGT00000000)

'Legionella Legionella longbeachae 12.7 [10.1 - 17.9 [15.8 - 13.1 [10.8 - 5.34 septentrionalis' NCTC 11477 16.0] 20.3] 15.9] (RZGT00000000)

'Legionella qingyii' Legionella septentrionalis 12.9 [10.2 - 17.8 [15.6 - 13.2 [10.9 - 4.32 (QHJG00000000) km711T 16.2] 20.1] 16.0]

'Legionella Legionella tucsonensis 12.7 [10.0 - 17.8 [15.7 - 13.1 [10.8 - 4.92 septentrionalis' ATCC 49180 16.0] 20.1] 15.9] (RZGT00000000)

'Legionella Legionella cherrii DSM 12.9 [10.2 - 17.8 [15.6 - 13.2 [10.9 - 3.47 septentrionalis' 19213 16.1] 20.1] 16.0] (RZGT00000000)

'Legionella Legionella qingyii CCTCC 12.8 [10.1 - 17.8 [15.6 - 13.2 [10.8 - 4.52 septentrionalis' AB 2018025 16.1] 20.1] 15.9] (RZGV00000000)

'Legionella qingyii' Legionella londiniensis 12.7 [10.0 - 17.8 [15.7 - 13.1 [10.7 - 5.48 (RZGX00000000) ATCC 49505 16.0] 20.2] 15.8]

'Legionella Legionella qingyii CCTCC 12.9 [10.2 - 17.8 [15.6 - 13.2 [10.9 - 4.36 septentrionalis' AB 2018025 16.2] 20.1] 16.0] (RZGS00000000)

'Legionella Legionella erythra ATCC 13.0 [10.3 - 17.8 [15.7 - 13.3 [11.0 - 4.36 septentrionalis' 35303 16.3] 20.2] 16.1] (RZGT00000000)

'Legionella Legionella wadsworthii 12.8 [10.1 - 17.8 [15.6 - 13.2 [10.8 - 4.35 septentrionalis' ATCC 33877 16.1] 20.1] 15.9] (RZGT00000000)

'Legionella qingyii' 'Legionella 12.9 [10.2 - 17.8 [15.6 - 13.2 [10.9 - 4.35 (QHJG00000000) septentrionalis' 16.2] 20.1] 16.0] (RZGS00000000)

'Legionella qingyii' 'Legionella 12.8 [10.1 - 17.8 [15.6 - 13.2 [10.8 - 4.51 (QHJG00000000) septentrionalis' 16.1] 20.1] 15.9] (RZGV00000000)

'Legionella Legionella gormanii ATCC 12.8 [10.2 - 17.7 [15.6 - 13.2 [10.9 - 4.12 septentrionalis' 33342 16.1] 20.0] 16.0] (RZGR00000000)

'Legionella Legionella saoudiensis 12.9 [10.2 - 17.7 [15.5 - 13.3 [10.9 - 2.9 septentrionalis' LH-SWC 16.2] 20.0] 16.0] (RZGT00000000)

'Legionella 'Legionella qingyii' 12.8 [10.1 - 17.7 [15.6 - 13.2 [10.8 - 4.38 septentrionalis' (RZGX00000000) 16.1] 20.1] 15.9] (RZGR00000000)

'Legionella 'Legionella qingyii' 12.9 [10.2 - 17.6 [15.5 - 13.2 [10.9 - 4.49 septentrionalis' (RZGX00000000) 16.2] 20.0] 16.0] (RZGV00000000) Query Subject d0 C.I. d0 d4 C.I. d4 d6 C.I. d6 Diff. G+C Percent

'Legionella Legionella rowbothamii 12.9 [10.2 - 17.6 [15.5 - 13.3 [10.9 - 2.83 septentrionalis' LLAP6 16.2] 19.9] 16.0] (RZGS00000000)

'Legionella Legionella cincinnatiensis 12.7 [10.0 - 17.6 [15.5 - 13.1 [10.7 - 5.55 septentrionalis' ATCC 43753 16.0] 20.0] 15.8] (RZGT00000000)

'Legionella Legionella worsleiensis 13.0 [10.2 - 17.6 [15.5 - 13.3 [10.9 - 2.02 septentrionalis' ATCC 49508 16.2] 20.0] 16.1] (RZGT00000000)

'Legionella Legionella gormanii ATCC 12.8 [10.1 - 17.6 [15.4 - 13.2 [10.8 - 4.25 septentrionalis' 33297 16.1] 19.9] 15.9] (RZGT00000000)

'Legionella Legionella rubrilucens 13.0 [10.3 - 17.6 [15.5 - 13.3 [11.0 - 5.33 septentrionalis' ATCC 35304 16.3] 20.0] 16.1] (RZGT00000000)

'Legionella 'Legionella qingyii' 12.8 [10.1 - 17.5 [15.4 - 13.2 [10.8 - 4.51 septentrionalis' (RZGW00000000) 16.1] 19.9] 15.9] (RZGT00000000)

'Legionella qingyii' Legionella septentrionalis 12.9 [10.2 - 17.5 [15.4 - 13.3 [10.9 - 4.31 (RZGX00000000) km711T 16.2] 19.8] 16.0]

'Legionella Legionella yabuuchiae 13.2 [10.4 - 17.5 [15.4 - 13.5 [11.1 - 1.36 septentrionalis' OA1-2 16.5] 19.8] 16.3] (RZGT00000000)

'Legionella Legionella pneumophila 12.9 [10.2 - 17.5 [15.3 - 13.2 [10.9 - 4.25 septentrionalis' subsp. pascullei ATCC 16.2] 19.8] 16.0] (RZGT00000000) 33737

'Legionella Legionella gormanii ATCC 12.8 [10.1 - 17.5 [15.4 - 13.2 [10.8 - 4.25 septentrionalis' 33342 16.1] 19.9] 15.9] (RZGT00000000)

'Legionella Legionella rowbothamii 12.9 [10.2 - 17.5 [15.4 - 13.3 [10.9 - 3.0 septentrionalis' LLAP6 16.2] 19.9] 16.0] (RZGT00000000)

'Legionella 'Legionella qingyii' 12.9 [10.2 - 17.5 [15.4 - 13.3 [10.9 - 4.34 septentrionalis' (RZGX00000000) 16.2] 19.8] 16.0] (RZGS00000000)

'Legionella Legionella yabuuchiae 13.3 [10.5 - 17.5 [15.4 - 13.6 [11.2 - 1.18 septentrionalis' OA1-2 16.6] 19.9] 16.4] (RZGS00000000)

'Legionella Legionella drancourtii 12.8 [10.1 - 17.4 [15.3 - 13.2 [10.8 - 3.09 septentrionalis' LLAP 12 16.1] 19.7] 15.9] (RZGT00000000)

'Legionella Legionella spiritensis 12.8 [10.1 - 17.4 [15.3 - 13.2 [10.8 - 3.06 septentrionalis' ATCC 35249 16.1] 19.7] 15.9] (RZGT00000000)

'Legionella sp. km772' Legionella spiritensis 12.9 [10.2 - 17.3 [15.2 - 13.3 [10.9 - 6.4 (RZGQ00000000) ATCC 35249 16.2] 19.6] 16.0]

'Legionella Legionella qingyii CCTCC 12.7 [10.1 - 17.2 [15.1 - 13.1 [10.8 - 4.53 septentrionalis' AB 2018025 16.0] 19.6] 15.9] (RZGT00000000)

'Legionella qingyii' 'Legionella 12.7 [10.1 - 17.2 [15.1 - 13.1 [10.8 - 4.53 (QHJG00000000) septentrionalis' 16.0] 19.6] 15.9] (RZGT00000000)

'Legionella 'Legionella qingyii' 12.8 [10.1 - 17.1 [15.0 - 13.2 [10.8 - 4.51 septentrionalis' (RZGX00000000) 16.1] 19.4] 15.9] (RZGT00000000) Table 4: Strains in your dataset Joint dataset of automatically determined closest type strains (if this mode was chosen), manually selected type strains (if selected accordingly) and the provided user strains, if provided (marked in yellow).

Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Orrison et al. CCUG Legionella 2773 40.9 2932 Gp0013845 PRJNA183980 SAMN02641506 GCA_000512355 2558860203 oakridgensis 1983 16414; oakridgensis 209 ATCC 33761 DSM 21215; NCTC 11531; CIP 103884; Oak Ridge 10; OR-10

Legionella Brenner et al. CCUG Legionella 3431 38.1 3048 Gp0133674 PRJNA239272 SAMN02650970 GCA_000586255 pneumophila 1989 31226 A; pneumophila 069 subsp. pascullei CCUG subsp. pascullei ATCC 33737 31226; DSM 7515; CIP 105570; U8W

Legionella Lo Presti et al. ATCC Legionella 3538 37.3 3125 Gp0384399 PRJEB6403 SAMEA4412675 GCA_900452395 beliardensis 2001 700512; beliardensis 304 NCTC 13315 DSM 19152; CIP 106632; liard A1; Montbeliar d A1; Montbéliar d A1

Legionella Li et al. 2021 KCTC Legionella 2651 42.1 2359 PRJNA224116 SAMN10610692 GCF_003989745 septentrionalis 15655; septentrionalis 472 km711T NBRC 113219

Legionella Verma et al. 214; ATCC Legionella 3508 41.7 2985 Gp0013848 PRJNA169766 SAMN02440432 GCA_000373765 2515154162 shakespearei 1992 49655; shakespearei 986 DSM 23087 CCUG 31228 A; CCUG 31228; NCTC 12829; CIP 103541 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella McKinney et al. ATCC Legionella 4121 37.0 3445 PRJNA510202 SAMN10599827 GCA_004283175 longbeachae 1982 33462; longbeachae 576 NCTC 11477 CCUG 46622; DSM 10572; CIP 103880; Long Beach 4

Legionella Lo Presti et al. ATCC Legionella 3151 48.1 2876 Gp0384400 PRJEB6403 SAMEA4412680 GCA_900452865 taurinensis 1999 700508; taurinensis 601 NCTC 13314 CCUG 44901; DSM 21897; CIP 106300; Turin I n° 1

Legionella Kuroki et al. DSM Legionella 2492 40.2 2316 PRJEB24896 SAMEA5198051 GCA_900639875 impletisoli OA1-1 2007 18493; impletisoli 103 JCM 13919; OA1-1

Legionella Palmer et al. Legionella 3272 39.4 2932 Gp0258950 PRJNA326348 SAMN05254214 GCA_002240035 clemsonensis 2016 clemsonensis 100 CDC-D5610

Legionella Wilkinson et al. 316-36; Legionella 3761 40.0 3219 Gp0013844 PRJNA185635 SAMN02441510 GCA_000423305 2522572178 moravica DSM 1989 ATCC moravica 766 19234 43877; CCUG 31117; NCTC 12239; CDC 1634; CDC 1634- CZK-E; CIP 103883

Legionella Morris et al. ATCC Fluoribacter 3812 38.1 3345 Gp0131767 PRJNA329785 SAMN05421777 GCA_900156395 2681812897 gormanii ATCC 1980 33297; gormanii; 314 33342 ATCC Legionella 33342; gormanii CCUG 12267; DSM 16641; DSM 25296; NCTC 11401; CIP 104724; LS-13 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Adeleke et al. ATCC Legionella 3966 39.3 3426 PRJEB24896 SAMEA5198062 GCA_900639985 rowbothamii 2001 700991; rowbothamii 228 LLAP6 CCUG 43886; DSM 21678

Legionella Kuroki et al. DSM Legionella 2638 41.0 2378 PRJEB24896 SAMEA5198072 GCA_900640115 yabuuchiae OA1- 2007 18492; yabuuchiae 659 2 JCM 14148

Legionella Yang et al. 2012 ATCC BAA- Legionella 2676 41.1 2534 PRJEB24896 SAMEA5198055 GCA_900639915 nagasakiensis 1557; CDC- nagasakiensis 583 JCM 15315 1796-JAP- E

Legionella Bajrai et al. 2016 DSM Legionella 3846 39.4 3341 Gp0143847 PRJEB11688 SAMEA3647790 GCA_001465875 saoudiensis LH- 101682; saoudiensis 014 SWC CSUR P2101; LS- 1

Legionella Campbell et al. CCUG Legionella 4108 37.0 3550 Gp0018889 PRJNA223118 SAMN02744009 GCA_000621685 2558309002 sainthelensi 1984 29672; sainthelensi 625 ATCC 35248 DSM 19231; NCTC 11988; CIP 103885; Mt. St. Helens 4

Legionella cherrii Brenner et al. ATCC Legionella cherrii 3698 38.9 3177 Gp0013839 PRJNA222237 SAMN02743988 GCA_000621385 2558309037 DSM 19213 1985 35252; 064 CCUG 29666; NCTC 11976; CIP 103842; ORW

Legionella Thacker et al. 1725-Aus- Legionella 2651 39.6 2506 Gp0044111 PRJNA223037 SAMN02743993 GCA_000621525 2558860982 fairfieldensis 1991 E; CCUG fairfieldensis 631 ATCC 49588 31235 A; CCUG 31235; NCTC 12488; CIP 105266 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Edelstein et al. CCUG Legionella 3573 38.0 3168 Gp0018919 PRJNA234416 SAMN02744130 GCA_000701265 2561511070 wadsworthii 1983 16415; wadsworthii 650 ATCC 33877 DSM 21896; NCTC 11532; CIP 103886; Wadsworth 81-716A

Tamlana Li et al. 2020 CICC Tamlana 3999 34.3 3345 PRJNA544675 SAMN11845908 GCA_006265225 fucoidanivorans 24749; fucoidanivorans 027 CW2-9T KCTC 72389

Legionella Brenner et al. ATCC Legionella 3408 39.4 3036 Gp0013842 PRJNA221073 SAMN02746073 GCA_900114725 2600255316 jamestowniensis 1985 35298; jamestowniensis 907 DSM 19215 CCUG 29669; JCM 7590; NCTC 11981; CIP 103845; JA-26-G1- E2

Legionella qingyii Wu et al. 2019 KCTC Legionella qingyii 5227 37.8 3955 Gp0389901 PRJNA465828 SAMN09090942 GCA_003184185 CCTCC AB 15636; 415 2018025 km488; NRBC 113223

Legionella Adeleke et al. ATCC Legionella 4434 38.3 3850 Gp0122638 PRJEB7322 SAMEA2785663 GCA_000953135 fallonii LLAP-10 2001 700992; fallonii 890 CCUG 43887; DSM 19889

Legionella Brenner et al. CCUG Legionella 3570 39.0 3218 Gp0122637 PRJEB7321 SAMEA2785664 GCA_000953655 hackeliae ATCC 1985 31232 A; hackeliae 857 35250 CCUG 31232; DSM 19214; JCM 7563; NCTC 11979; CIP 103844; Lansing 2

Legionella Rizzardi et al. ATCC BAA- Legionella 3070 37.5 2338 Gp0094869 PRJNA246269 SAMN02746164 GCA_000770585 norrlandica 2015 2678; norrlandica 847 LEGN CCUG 65936 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Campocasso et DSM Legionella 4387 41.2 3767 Gp0044362 PRJEB6598 SAMEA2747211 GCA_000756815 massiliensis al. 2012 24804; massiliensis 007 LegA CSUR P146

Legionella (Garrity et al. ALLO1; Fluoribacter 4113 37.9 3624 PRJNA281295 SAMN03486950 GCA_001648595 bozemanae 1980) Brenner et DSM bozemanae; 435 ATCC 33217 al. 1980 16523; CIP Legionella 103872; bozemanae WIGA

Legionella Cherry et al. CCUG Legionella 3087 41.7 2825 PRJNA281299 SAMN03486956 GCA_001648675 jordanis ATCC 1982 16413; jordanis 298 33623 DSM 19212; NCTC 11533; BL- 540; CIP 105268

Legionella Morris et al. ATCC Fluoribacter 3812 38.1 3301 PRJNA281301 SAMN03486957 GCA_001648685 gormanii ATCC 1980 33297; gormanii; 103 33297 ATCC Legionella 33342; gormanii CCUG 12267; DSM 16641; DSM 25296; NCTC 11401; CIP 104724; LS-13

Legionella Brenner et al. ATCC Fluoribacter 3894 39.6 3449 Gp0012805 PRJNA74015 SAMN02469353 GCA_000236165 2547132117 dumoffii NY 23 1980 33279; dumoffii; 641 CCUG Legionella 11881; dumoffii DSM 17625; NCTC 11370; CIP 103876

Legionella Kuroki et al. DSM Legionella 2485 40.2 2291 PRJDB10510 SAMD00245244 GCA_014646855 impletisoli JCM 2007 18493; impletisoli 448 13919 JCM 13919; OA1-1

Legionella La Scola et al. ATCC Legionella 8140 39.2 3959 Gp0004932 PRJNA39279 SAMN02471332 GCA_000162755 645058794 drancourtii LLAP 2004 50991 drancourtii 168 12 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Brenner et al. ATCC Legionella 4232 38.0 3916 Gp0378973 PRJNA312141 SAMN04495209 GCA_001736145 parisiensis DSM 1985 35299; parisiensis 284 19216 CCUG 29670; JCM 7561; NCTC 11983; CIP 103847; PF-209C- C2

Legionella anisa Gorman et al. CCUG Legionella anisa 4396 38.2 3732 Gp0133607 PRJNA224116 SAMN04274764 GCF_001467525 ATCC 35292 1985 29665; 893 DSM 17627; JCM 7573; NCTC 11974; CIP 103870; WA-316-C3

Legionella Bornstein et al. CCUG Legionella 3924 36.8 3446 Gp0132661 PRJNA224116 SAMN04274776 GCF_001467695 gratiana ATCC 1991 44894; gratiana 747 49413 DSM 21233; NCTC 12388; CDC 1242; CIP 105267; Lyon 8420412

Legionella Wilkinson et al. 441-1; Legionella 3748 38.7 3170 Gp0132962 PRJNA224116 SAMN04274767 GCF_001467025 brunensis ATCC 1989 CCUG brunensis 018 43878 31114; DSM 19236; NCTC 12240; CDC 1635; CIP 103874

Legionella Dennis et al. 1477; Legionella 2400 43.3 2129 Gp0133319 PRJNA224116 SAMN04274782 GCF_001467825 londiniensis 1993 CCUG londiniensis 013 ATCC 49505 44895; DSM 21234; NCTC 12374; CIP 105269 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Wilkinson et al. 1407-AL-H; Legionella 3736 43.5 3235 Gp0133232 PRJNA224116 SAMN04274765 GCF_001467505 birminghamensis 1988 CCUG birminghamensis 683 ATCC 43702 31233 A; CCUG 31233; DSM 19232; JCM 7559; NCTC 12437; CIP 103871

Legionella Brenner et al. CCUG Legionella 3237 46.7 2961 Gp0133677 PRJNA224116 SAMN04274772 GCF_001467615 erythra ATCC 1985 29667; erythra 543 35303 DSM 17644; JCM 7564; NCTC 11977; CIP 103843; SE-32A-C8

Legionella Brenner et al. CCUG Legionella 3128 47.7 2874 Gp0132663 PRJNA224116 SAMN04274791 GCF_001468125 rubrilucens 1985 29671; rubrilucens 807 ATCC 35304 DSM 11884; JCM 7565; NCTC 11987; CIP 103848; WA-270A- C2

Legionella Brenner et al. CCUG Legionella 4818 36.7 4254 Gp0133608 PRJNA224116 SAMN04274793 GCF_001468135 santicrucis ATCC 1985 29673; santicrucis 052 35301 DSM 19325; DSM 23075; JCM 7557; NCTC 11989; CIP 103849; SC-63-C7 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Brenner et al. CCUG Legionella 3312 45.4 2944 Gp0133505 PRJNA224116 SAMN04274795 GCF_001468165 spiritensis ATCC 1985 29715; spiritensis 138 35249 CCUG 31118; DSM 19324; JCM 7562; NCTC 11990; CIP 103850; Mount Saint Helens 9; Mt. St. Helens 9

Legionella steelei Edelstein et al. IMVS 3113; Legionella steelei 4227 38.8 3716 Gp0133443 PRJNA224116 SAMN04274796 GCF_001468005 ATCC BAA-2169 2012 IMVS 3376 062

Legionella Brenner et al. CCUG Legionella 3948 38.3 3471 Gp0133609 PRJNA224116 SAMN04274797 GCF_001468065 steigerwaltii 1985 29674; steigerwaltii 340 ATCC 35302 DSM 23076; JCM 7558; NCTC 11991; CIP 103851; SC-18-C9

Legionella Benson et al. 2074-AUS- Legionella 3648 39.1 3387 Gp0132986 PRJNA224116 SAMN04274799 GCF_001468085 waltersii ATCC 1996 E; CCUG waltersii 718 51914 44924; DSM 21908; CIP 104965

Legionella Brenner et al. ATCC Legionella 3397 38.3 2942 Gp0000541 PRJNA22 SAMN02603156 GCA_000008485 637000148 pneumophila 1979 33152; pneumophila; 753 Philadelphia 1 CCUG Legionella 9568; DSM pneumophila 25069; subsp. DSM 7513; pneumophila JCM 7571; NCTC 11192; CIP 103854

Legionella Dennis et al. 1335; Legionella 4197 39.0 3522 Gp0133462 PRJNA224116 SAMN04274789 GCF_001467955 quateirensis 1993 CCUG quateirensis 232 ATCC 49507 44899; DSM 21253; NCTC 12376; CIP 105271 Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

Legionella Dennis et al. 1347; Legionella 3065 40.3 2643 Gp0133187 PRJNA224116 SAMN04274800 GCF_001467535 worsleiensis 1993 CCUG worsleiensis 894 ATCC 49508 44923; DSM 21907; NCTC 12377; CIP 105114

Legionella Brenner et al. CCUG Legionella 2203 37.8 1968 Gp0133226 PRJNA224116 SAMN02650967 GCF_000586315 pneumophila 1989 13398; pneumophila 310 subsp. fraseri DSM 7514; subsp. fraseri ATCC 33156 NCTC 11233; CIP 103858; Los Angeles 1; Los Angelos-1

Legionella Thacker et al. 1087-AZ-H; Legionella 3356 37.4 2965 Gp0133365 PRJNA224116 SAMN04274798 GCF_001468035 tucsonensis 1990 CCUG tucsonensis 307 ATCC 49180 31119; DSM 19246; NCTC 12439; CIP 105113

Legionella Thacker et al. 72-OH-H; Legionella 4052 36.8 3467 Gp0132660 PRJNA224116 SAMN04274769 GCF_001467545 cincinnatiensis 1989 CCUG cincinnatiensis 168 ATCC 43753 31230 A; CCUG 31230; DSM 19233; NCTC 12438; CIP 103875

'Legionella 5241 37.8 4197 qingyii' 559 (QHJG00000000 )

'Legionella sp. 3407 39.0 3274 km772' 521 (RZGQ00000000 )

'Legionella 2569 42.2 2411 septentrionalis' 255 (RZGR00000000 ) Strain Authority Other Synonyms Base Percent No. Goldstamp Bioproject Biosample Assembly IMG OID deposits pairs G+C proteins accession accession accession

'Legionella 2660 42.2 2491 septentrionalis' 547 (RZGS00000000 )

'Legionella 2445 42.3 2266 septentrionalis' 666 (RZGT00000000 )

'Legionella sp. 3677 39.9 3125 km535' 876 (RZGU00000000 )

'Legionella 2545 42.3 2366 septentrionalis' 809 (RZGV00000000 )

'Legionella 3721 37.8 3225 qingyii' 623 (RZGW00000000 )

'Legionella 3808 37.8 3314 qingyii' 297 (RZGX00000000 )

'Legionella 4095 36.8 3511 sainthelensi' 037 (UFAL00000000)

'Legionella 4516 36.8 3990 sainthelensi' 585 (UFAM00000000 ) Methods, Results and References The genome sequence data were uploaded to the Type (Strain) Genome Server (TYGS), a free bioinformatics platform available under https://tygs.dsmz.de, for a whole genome-based taxonomic analysis [1]. The results were provided by the TYGS on 2021-03-27. The TYGS analysis was subdivided into the following steps:

Determination of closely related type strains Determination of closest type strain genomes was done in two complementary ways: First, all user genomes were compared against all type strain genomes available in the TYGS database via the MASH algorithm, a fast approximation of intergenomic relatedness [2], and, the ten type strains with the smallest MASH distances chosen per user genome. Second, an additional set of ten closely related type strains was determined via the 16S rDNA gene sequences. These were extracted from the user genomes using RNAmmer [3] and each sequence was subsequently BLASTed [4] against the 16S rDNA gene sequence of each of the currently 15524 type strains available in the TYGS database. This was used as a proxy to find the best 50 matching type strains (according to the bitscore) for each user genome and to subsequently calculate precise distances using the Genome BLAST Distance Phylogeny approach (GBDP) under the algorithm 'coverage' and distance formula d5 [5]. These distances were finally used to determine the 10 closest type strain genomes for each of the user genomes.

Pairwise comparison of genome sequences For the phylogenomic inference, all pairwise comparisons among the set of genomes were conducted using GBDP and accurate intergenomic distances inferred under the algorithm 'trimming' and distance formula d5 [5]. 100 distance replicates were calculated each. A second GBDP phylogenomic analysis was inferred using the amino acid sequences of the entire proteome, promising a better resolved phylogeny in datasets of only remotely related strains. Digital DDH values and confidence intervals were calculated using the recommended settings of the GGDC 2.1 [5].

Phylogenetic inference The resulting intergenomic distances were used to infer a balanced minimum evolution tree with branch support via FASTME 2.1.6.1 including SPR postprocessing [6]. Branch support was inferred from 100 pseudo- bootstrap replicates each. The trees were rooted at the midpoint [7] and visualized with PhyD3 [8].

Type-based species and subspecies clustering The type-based species clustering using a 70% dDDH radius around each of the 51 type strains was done as previously described [1]. The resulting groups are shown in Table 1 and 4. Subspecies clustering was done using a 79% dDDH threshold as previously introduced [9].

Results

Type-based species and subspecies clustering The resulting species and subspecies clusters are listed in Table 4, whereas the taxonomic identification of the query strains is found in Table 1. Briefly, the clustering yielded 51 species clusters and the provided query strains were assigned to 5 of these. Moreover, user strains were located in 6 of 53 subspecies clusters.

Figure caption SSU tree Figure 1. Tree inferred with FastME 2.1.6.1 [6] from GBDP distances calculated from 16S rDNA gene sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values > 60 % from 100 replications, with an average branch support of 61.3 %. The tree was rooted at the midpoint [7].

Figure caption genome tree Figure 2. Tree inferred with FastME 2.1.6.1 [6] from GBDP distances calculated from genome sequences. The branch lengths are scaled in terms of GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values > 60 % from 100 replications, with an average branch support of 37.6 %. The tree was rooted at the midpoint [7].

Figure caption proteome tree Figure 3. Tree inferred with FastME 2.1.6.1 [6] from whole-proteome-based GBDP distances. The branch lengths are scaled via GBDP distance formula d5. Branch values are GBDP pseudo-bootstrap support values > 60 % from 100 replications, with an average branch support of 91.4 %. The tree was midpoint-rooted [7]. References [1] Meier-Kolthoff JP, Göker M. TYGS is an automated high-throughput platform for state-of-the-art genome- based taxonomy. Nat. Commun. 2019;10: 2182. DOI: 10.1038/s41467-019-10210-3

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