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ASAIO 2018 Annual Meeting Abstracts. Washington, DC

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ASAIO 2018 Annual Meeting Abstracts. Washington, DC May-June 2018 • Volume 64 • Supplement 1 www.asaiojournal.com ISSN 1058-2916 05/21/2018 on BhDMf5ePHKav1zEoum1tQfN4a+kJLhEZgbsIHo4XMi0hCywCX1AWnYQp/IlQrHD3be73+7zLk/Sq+y6koaZl6Ep8oGuop87+8AoBvh7J400mYizg71tReg== by https://journals.lww.com/asaiojournal from Downloaded Downloaded from https://journals.lww.com/asaiojournal by BhDMf5ePHKav1zEoum1tQfN4a+kJLhEZgbsIHo4XMi0hCywCX1AWnYQp/IlQrHD3be73+7zLk/Sq+y6koaZl6Ep8oGuop87+8AoBvh7J400mYizg71tReg== The Official Publication of the American Society for Artificial Internal Organs ASAIO Promotes the Development, Application, and Awareness of Organ Technologies that Enhance Quality and Duration of Life Washington, DC ASAIO 64th Annual Conference June 13-16, 2018 on 05/21/2018 ASAIO 2018 Annual Meeting Abstracts. Published for the Society by May/June 2018 Volume 64 • Supplement 1 ISSN 1058-2916 Bioengineering Abstracts .....................................................................................Pages 1-39 Cardiac Abstracts ............................................................................................Pages 40-101 Nursing Circ-Support Abstracts .....................................................................Pages 102-113 Pediatric Abstracts ........................................................................................Pages 114-122 Pulmonary Abstracts .....................................................................................Pages 123-130 Renal Abstracts .............................................................................................Pages 131-134 Author Index .................................................................................................Pages 135-144 ASAIO BIOENGINEERING ABSTRACTS 7 18 An Alternative Production Method For Collagen To Obtain Scaffolds Evaluation Of The Potential Of 3d-Membranes For Artificial Lungs I. Derman, C. Arslan, M. Kazancı. Bahçeşehir University, Istanbul, F. Hesselmann,1 F. Brennecke,1 S. Djeljadini,2 S. Klein,3 C. Donay,4 C. TURKEY. Cornelissen,5 U. Steinseifer,1 J. Arens1. 1Department of Cardiovascular Study: Collagen is a basic structural element in native extra cellular Engineering, Institute of Applied Medical Engineering, Aachen, 2 matrices, and its abundant presence in natural tissues, composing 30% by GERMANY, Department of Chemical Process Engineering, Aachener 3 weight of body protein tissues1, predestines it as a polymer for biomedi- Verfahrenstechnik, Aachen, GERMANY, Department of Tissue Engineering cal materials and tissue engineering matrices. It is generally extracted and Textile Implants, Institute of Applied Medical Engineering, Aachen, 4 from the natural tissues by treatments with acid or alkali, enzyme, and GERMANY, Institut für Textiltechnik Aachen, Aachen, GERMANY, 5 microorganisms2. However these methods are generally depend on batch Department for Internal Medicine - Section for Pneumology, Uniklinik type and reactants, time and energy consuming, and highly costly meth- RWTH Aachen, Aachen, GERMANY. ods. In this paper, we discuss an alternative method that could be applied Study: Until today hollow fibers are the state of the art core element of on different tissues to extract collagen. It decreases the time and energy extracorporal membrane oxygenator (ECMO) devices, which are restricted consumption and the usage of environment hazardous chemicals. to short term applications up to a few weeks. Over the last decade, Methods: In this study, we developed an improved method that reduces substantial improvements in 3D-printing technology were achieved that the time needed to extract this protein and increase the efficiency allows the fabrication of novel three-dimensional membranes with almost (Figure 1). The results were compared with the one obtained from the no limitations in shape and design. 3D-membranes with a blood flow traditional methods. The alternative method uses traditional extraction adapted design promise low pressure loss paired with high gas transfer buffers combined with forceful agitation and centrifugal filtration to efficiency and better hemocompatibility. Subject of this study is the evalu- obtain highly-pure, soluble collagen extraction. ation of the potential of three-dimensional membranes for artificial lungs. Results: This method is simple to perform using standard methods and Methods: The membrane designs under investigations come in the form equipment found in many laboratories. By employing high-speed agita- of triply periodic minimal surfaces. Initially, Schwarz-P, Schwarz-D and AHead=BHead=AHead=BHeadAfterAHead tion, this protocol reduces the time necessary to isolate solution, collagen Gyroid geometry were parametrized using CAD tools. Computational BHead=CHead=BHead=CHeadAfterBHead extraction from approximately 7 days to less than 3 hr.3 fluid dynamics simulations were combined with a gas transfer model to Conclusions: This paper indicates that these waste materials of animals estimate oxygenation performance, pressure loss and hemocompatibility CHead=DHead=CHead=DHeadAfterCHead have potential in supplementing the skin of land vertebrates as a source of the proposed 3D-membranes. In addition, the results are compared AHead=BHead=CHead=AHead=BHeadAfterAHead=CHeadAfterBHead of collagen. The end product (collagen) could be used in many different with a hollow fiber reference geometry. Design of experiments (DoE) BHead=CHead=DHead=BHead=CHeadAfterBHead=DHeadAfterCHead applications, ranging from drug carrier systems to tissue scaffolds and and multi-objective optimization was used to infer optimal scaling and reconstructive surgery. geometry parameters for each of the stated designs for low flow and high AHead=BHead=CHead=DHead=AHead=BHeadAfterAHead=CHeadAfterBHead=DHeadAfterCHe flow. Mesh independency was assured prior to the study. The hydraulic ad performance of the initial and optimized membrane designs was tested in vitro. Results: All suggested designs indicate a lower pressure drop and lower shear stress values. After optimization, the Schwarz-P design outper- forms the hollow fiber configuration by 44% at high flow and almost 94% at low flow in gas transfer efficiency according to the numerical results. However, a comparable high ratio of stagnation volume occurs. 3D-Membranes were evaluated numerically regarding their potential use in artificial lungs. According to the results the 3D-membranes possess high potential as core technology for more efficient and probably more hemocompatible artificial lungs. ; : – . Submitted for consideration ; accepted for publication in revised form . 1 ASAIO BIOENGINEERING ABSTRACTS 27 Transmission Electron Microscopy Of Explanted Intravascular Medical Devices S. L. Jessen,1 M. C. Friedemann,1 R. Nichols,2 B. R. Weeks,1 F. J. Clubb, Jr. 1. 1Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 2Texas Heart Institute, Houston, TX. Study: Plastic embedding and micro-ground sectioning (vs. traditional paraffin histology) is often preferred for histology analysis of implanted medical devices. In devices with metal and hard plastic components, micro-grinding preserves the tissue-device interface, allowing for assess- ment of tissue reaction and ingrowth into the device. However, the plastic embedding medium used to create micro-ground sections may prevent penetration of some stains, which results in inconsistent staining that can preclude a thorough pathology analysis of tissue substrate morphology. To combat this issue, transmission electron microscopy (TEM) can be used on targeted areas within micro-ground, plastic-embedded samples to validate light microscopic findings based on substrate morphology. Methods: Formalin-fixed vessels containing implanted occlusion devices were embedded in hard plastic resin and micro-ground to ~60 μm-thick sections. Slides were stained and evaluated using light microscopy. After Fibrin was also identified by TEM in other regions of ECM in which light identification of targeted region(s) of extracellular matrix (ECM), the microscopy trichrome staining was unclear. These findings can be used plastic embedded section was remounted to accommodate ultra-thin to more accurately assess tissue substrate morphology and the heal- ~80A sectioning. Ultra-thin sections were mounted and stained with lead ing response of host tissues to the medical device. This method can be citrate and uranyl acetate for TEM. ECM morphology was evaluated in applied to micro-ground sections of other hard-substance containing similar regions with both TEM and light microscopy. specimens in which a distinction between histologically similar materials Results: In micro-ground sections, trichrome inconsistently stains collagen (e.g., fibrin vs. collagen) is important. (Fig A); however, the same region of ECM ultrastructurally is identified as collagen (Fig B; 5000x). 2 ASAIO BIOENGINEERING ABSTRACTS 31 37 Modified Cavopulmonary Assist Device Implemented In The Inferior Predicting Plasma Free Hemoglobin Levels In Patients Due To Medical Vena Cava Can Improve Fontan Hemodynamics Device Hemolysis M. Farahmand, E. Kung. Mechanical Engineering, Clemson University, R. Malinauskas, D. Saylor, P. Buehler, R. Brown. US Food & Drug Clemson, SC. Administration, Silver Spring, MD. Study: Single ventricle patients typically undergo the Fontan palliation Study: Blood passage through medical devices can cause hemolysis and surgery. In this procedure the systemic venous blood flow is rerouted increased plasma free hemoglobin (pfH), which may lead to adverse directly to the lungs without
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