2008 Bitterroot Mountains Bear DNA Survey
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Bitterroot Mountains Bear DNA and Camera Survey: 2008‐2009 December 2010 Christopher Servheen, U.S. Fish and Wildlife Service, College of Forestry and Conservation, University of Montana, Missoula, Mt 59812 [email protected] Fax: 406‐329‐3212 Rebecca Shoemaker, College of Forestry and Conservation, University of Montana, Missoula, Mt 59812 [email protected] Bitterroot DNA Survey 2008‐2009 Final Report December 2010 BACKGROUND The grizzly bear (Ursus arctos horribilis) was listed as threatened under the Endangered Species Act in 1975. At that time, the Bitterroot ecosystem was identified as an area containing grizzly bears (41 FR 31734). After further investigation, in 1996, the US Fish and Wildlife Service determined that the best available scientific data indicated there was not a population of grizzly bears in the Bitterroot ecosystem (USFWS 1996). The Service released a supplemental chapter to its 1993 Grizzly Bear Recovery Plan detailing specific efforts in the Bitterroot ecosystem needed to achieve recovery. Following the recommendations of this supplemental Recovery Plan chapter, the Service initiated an environmental impact analysis (EIS) to assess the feasibility of implementing recovery actions in the Bitterroot ecosystem. The Record of Decision (ROD) was released in November 2000, with the selected alternative being, “Restoration of grizzly bears as a nonessential experimental population with citizen management.” (USFWS 2000). The ROD was never funded however it remains valid. Until September 2007, it was widely accepted that there were no grizzly bears in the Bitterroot ecosystem. On September 3, 2007, a young male grizzly bear was mistakenly killed by a black bear hunter in the northern Bitterroot ecosystem of Idaho a few miles from the Idaho/Montana border. Using this bear’s unique genetic signature, we determined it originated in the Selkirk Ecosystem nearly 140 air miles away. After confirming the identity and origin of the grizzly bear, many questions were raised. For instance, does this single, male bear mean there is a population in the Bitterroot ecosystem or was he just a transient passing through? The Final EIS defined a population in the following way: “…verified evidence within the previous six years, consisting of photos within the area, verified tracks and/or sightings by reputable scientists or agency personnel, of at least two different female grizzly bears with young or one female seen with different litters in two different years in an area geographically distinct from other grizzly bear populations.” (USFWS 2000). The Endangered Species Act defines a 10(j) “non‐essential experimental” population as one that is “…wholly separate geographically from non‐ experimental populations of the same species.” To make an informed decision based on the best available science about whether the Bitterroot Ecosystem meets this definition and to 2 Bitterroot DNA Survey 2008‐2009 Final Report December 2010 document the presence of any other grizzly bears in the Bitterroot Ecosystem; we surveyed the northern Bitterroots for grizzly bear presence using DNA barbed‐wire hair stations and motion‐ triggered digital cameras. STUDY AREA The study area in 2008 consisted of the northern Bitterroot Mountains between U.S. Highway 12 and Interstate 90 in areas in both Idaho and Montana (Figures 1, 2, 3). The effort was focused in National Forest lands near the Kelly Creek drainage, where the grizzly bear was killed in 2007. These forests included the Clearwater National Forest (ID), the St. Joe District of the Idaho Panhandle National Forest (ID) and the Lolo National Forest (MT). In 2009, we expanded our sampling effort to include 2 main study areas: 1) the Clark Fork Study Area, and 2) the Bitterroot Study Area (Figures 2, 3). The Clark Fork Study Area includes the northern Bitterroot Mountains of Montana south of the Clark Fork River between St. Regis and the Idaho/Montana state line. Our sites were primarily located on the Kootenai (MT) and Lolo (MT) National Forests with 2 sites on the Coeur d’Alene District of the Idaho Panhandle National Forest (ID). The Bitterroot Study Area in 2009 was a continuation of our 2008 sampling effort in the northern Bitterroot Mountains between the Idaho/Montana state line and US Hwy. 12 in Idaho. This study area is within the Clearwater National Forest (ID) and the St. Joe District of the Idaho Panhandle National Forest (ID). METHODS Sampling Strategy. In 2008, we placed as many hair stations as possible over the course of a short field season (July‐August). In doing so, ease of access (rock slides, snow banks, proximity to trails, etc.) influenced our site placement to allow us to put multiple sites out in a single day. We left these sites out for 1 sampling session (approximately 14 days), then removed them and re‐set them at a new location. 3 Bitterroot DNA Survey 2008‐2009 Final Report December 2010 In contrast, in 2009, we had two different sampling periods. We sampled the Clark Fork Study Area in the early season (May‐June) while we sampled the Bitterroot Study Area during July‐ August (Figure 2). In the Clark Fork Study Area, we used a similar approach to 2008 and placed the maximum number of sites possible out for 14 day sessions, then moved these to new locations for the next sampling session. In the Bitterroot Study Area, we attempted to reduce the overall quantity of sites while improving the quality of sites. We re‐visited areas that were successful in photographing multiple bears and collecting bear hair in 2008, placed sites in large areas that lacked roads and trails, and attempted to select locations that were “natural movement areas” for wide‐ranging mammals. These included confluences of major or multiple drainages (e.g., Canyon Creek x Little N. Fk. Clearwater) and major mountain saddles (e.g., Pole Mountain saddle). For these 2009 Bitterroot Study Area sites, we collected hair and re‐baited them every 2 weeks so that they were on the landscape at the same location for periods ranging from 2‐6 weeks. Site Selection. Our rationale for choosing sites was based on several factors. We consulted with local biologists, managers, and conservation officers from the Great Burn Study Group, the Idaho Department of Fish and Game, the Montana Department of Fish Wildlife and Parks, and the U.S. Fish and Wildlife Service (USFWS) to locate promising areas. Other macro‐site selection factors included natural movement areas such as saddles, drainages, finger ridges, and confluences; relative levels of human access; and landscape scale wind movement patterns. Micro‐site selection was based on availability of bear foods, proximity to trail junctions, proximity to creek confluences, landscape heterogeneity, wind dispersal, and proximity to water sources. Specific site locations were chosen on the ground by field crews trained to identify good quality grizzly bear habitat, foods, bear sign, and movement areas. Individual sites were placed at least 500m from any road or developed site and at least 100m from any trail. Hair Collection. Collection of bear hair with barbed‐wire was based on protocols verified to be effective in snagging bear hair (see Woods et al. 1999, Kendall et al. 2009). At each station, 4 Bitterroot DNA Survey 2008‐2009 Final Report December 2010 sites consisted of a non‐reward lure pile encircled by barbed wire. Lure piles were composed of local organic matter such as rotten logs and moss and were piled approximately 1m high and 1m wide and doused with approximately 2 liters of non‐reward lure solution. The barbed‐wire was >2m from the lure pile on all sides and 45cm above the ground. A cloth soaked in lure was hung above the site to disperse the lure scent further. Motion triggered cameras were placed at as many sites as possible. We used Reconyx PC‐85 cameras because of their quick “trigger time” (time between a trigger event and photo being taken), long battery life, portability, and reliability. Because we had only 30 cameras, it was not possible to place cameras at all sites when more than 30 sites were out at a single time. We attempted to revisit sites every 14 days to collect hair samples and re‐bait or dismantle the site but this sampling session time varied from 12‐16 days depending on logistics and scheduling. Each barb was treated as a single sample and collected in its own coin envelope. Both the barb and the tweezers used to collect samples were burned after collecting each sample to avoid cross‐contamination. Trees used as part of the corral, the ground below the barbed‐wire, and the lure pile were also checked for hair. Genetic Analysis. All samples were transported to Wildlife Genetics International (WGI) in Nelson, B.C. by a USFWS employee under a CITES (Convention on International Trade in Endangered Species) permit. Samples were visually inspected initially and then screened for species identification using a sequence‐based mtDNA test. In the event that grizzly bear samples were detected, individual ID would be established using a standardized set of 7 microsatellite markers that are highly variable in grizzly bear populations in the lower‐48 States. Error‐checking followed a standard protocol (Paetkau 2003). If grizzly bear samples were identified, WGI would use an assignment test to determine where the bear came from based on its unique genetic signature (Paetkau et al. 1995; Waser and Strobeck 1998; Paetkau et al. 2004). 5 Bitterroot DNA Survey 2008‐2009 Final Report December 2010 To assess the effectiveness of our 2009 Bitterroot Study Area sampling strategy, we genotyped a subset of black bear samples from sites to attempt to answer the following questions and inform future study designs: 1. For sites that were re‐baited, did we get different individuals in subsequent sessions or the same individual re‐visiting? 2.