Establecimiento Del Calendario Polínico De La Atmósfera Del Bosque De Chapultepec, Y Detección De Las Proteínas De Granos De Polen De Fraxinus Spp

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Establecimiento Del Calendario Polínico De La Atmósfera Del Bosque De Chapultepec, Y Detección De Las Proteínas De Granos De Polen De Fraxinus Spp UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO POSGRADO EN CIENCIAS BIOLÓGICAS GEOLOGÍA Biología Experimental Establecimiento del calendario polínico de la atmósfera del Bosque de Chapultepec, y detección de las proteínas de granos de polen de Fraxinus spp. en muestras de aire. TESIS QUE PARA OPTAR POR EL GRADO DE: MAESTRA EN CIENCIAS BIOLÓGICAS PRESENTA: Tania Robledo Retana TUTORA PRINCIPAL DE TESIS: Dra. María del Carmen Leticia Calderón Ezquerro Centro de Ciencias de la Atmósfera COMITÉ TUTOR: Dra. María Hilda Flores Olvera Instituto de Biología Dr. Francisco Jesús Arenas Huertero Facultad de Medicina TUTORA INVITADA: Dra. María del Rocío Alicia Reyes Montes Facultad de Medicina MÉXICO, D.F. DICIEMBRE, 2013 UNAM – Dirección General de Bibliotecas Tesis Digitales Restricciones de uso DERECHOS RESERVADOS © PROHIBIDA SU REPRODUCCIÓN TOTAL O PARCIAL Todo el material contenido en esta tesis esta protegido por la Ley Federal del Derecho de Autor (LFDA) de los Estados Unidos Mexicanos (México). El uso de imágenes, fragmentos de videos, y demás material que sea objeto de protección de los derechos de autor, será exclusivamente para fines educativos e informativos y deberá citar la fuente donde la obtuvo mencionando el autor o autores. Cualquier uso distinto como el lucro, reproducción, edición o modificación, será perseguido y sancionado por el respectivo titular de los Derechos de Autor. UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO POSGRADO EN CIENCIAS BIOLÓGICAS GEOLOGÍA Biología Experimental Establecimiento del calendario polínico de la atmósfera del Bosque de Chapultepec, y detección de las proteínas de granos de polen de Fraxinus spp. en muestras de aire. TESIS QUE PARA OPTAR POR EL GRADO DE: MAESTRA EN CIENCIAS BIOLÓGICAS PRESENTA: Tania Robledo Retana TUTORA PRINCIPAL DE TESIS: Dra. María del Carmen Leticia Calderón Ezquerro Centro de Ciencias de la Atmósfera COMITÉ TUTOR: Dra. María Hilda Flores Olvera Instituto de Biología Dr. Francisco Jesús Arenas Huertero Facultad de Medicina TUTORA INVITADA: Dra. María del Rocío Alicia Reyes Montes Facultad de Medicina MÉXICO, D.F. DICIEMBRE, 2013 UN MD COORDINACiÓN POSG DO ~ Dr. Isidro Áv ila Martínez Directo r General de Administración Escolar, UNAM Pr esen t e Me permito informar a usted que en la reunión de subcomité de Ecologla y Manejo Integral de Ecosistemas del Posgrado en Ciencias BiolOgicas, celebrada el dla 28 de octubre. se aprobó el siguiente jurado para el examen de grado de MAESTRA EN CIENCIAS BIOLÓGICA S de la alumna ROB LEDO RETANA TANIA con numero de cuenta 512012586 con la tesis títulada " Establecimiento dol calendario polínico de la atmósfera del Bosque de Chapultepec y detección de proteín as d e g ranos de polen de Fraxinus spp. en muestras de aire.", realizada bajo la dirección de la DRA. MARIA DEL CARMEN CALDERO N EZQUERRO: Presidente: ORo EDGAR ARTURO ZENTENO GAUNOO Vocal: ORo YOHAN ALEXANDER CORREA METRIO Secretario. ORA. MARIA HllDA FLORES OL VERA Suplente : DRA. MARIA ELENA CALDERÓN SEGURA Suplente: OR. RICARDO LASCURAIN LEOESMA Sin otro particular, me es grato enviarle un cordial saludo. ATENT AM EN TE " POR MI RAZA HABLARÁ EL EspiRITU" Cd. Universitaria , D.F., a 21 de noviembre de 2013. c.c.p. Expediente Unid.'ld de Posgrado • Coo rd.i~ .'l ció n del Posgrudo en Ciencias Bi ológicas Edificio B, I er. Pi so, Circuito de Posgmdos ('d. Uni versiWria DelegaClOTl Coyoncnn C.P. 045 10 México. D.F. TeL 5623 7002 hllp:l/pcbioLposgrado.unam.mx Al Posgrado en Ciencias Biológicas, UNAM, por permitirme realizar el estudio de maestría en la UNAM A la Universidad Autónoma de México (UNAM), por permitirme estudiar en esta institución Al Consejo Nacional de Ciencia y Tecnología (CONACYT), por el apoyo monetario recibido con la beca de maestría (CVU/becario) 312407/262391 durante 2011-2013. Al Centro de Ciencias de la Atmósfera por permitir la realización de este estudio, así como los apoyos otorgados a estudiantes y a la REMA para que sea posible que las estaciones de muestreo continúen funcionando. Al Programa de Apoyo para los Estudios de Posgrado (PAEP) de la UNAM, por el apoyo para la asistencia a dos congresos del PAAA (PanAmerican Aerobiology Association) en 2012 y 2013. Así como el apoyo otorgado para la impresión de tesis. A la Agencia Española de Cooperación Internacional para el Desarrollo (AECID), por los apoyos, para las estancias realizadas al Departamento de Biología Vegetal de la Universidad de Málaga (2008 - 2013) A PAPIIT por el apoyo al Proyecto de la REMA, con el número IN201109-3 (2009 y renovado en 2010 y 2011). Al Centro Virtual de Cambio Climático (2008-2009) A la Secretaría de Ciencia, Tecnología en Innovación del Distrito Federal (anteriormente ICyTDF) A todos los Doctores que formaron parte de mi comité tutoral: La Dra. Calderón Ezquerro, por darme la oportunidad de iniciar este proyecto con ella. Así como compartir su tiempo y experiencia, para que este proyecto saliera adelante. A la Dra. Hilda Flores por su tiempo para formar parte del comité tutoral, además de las ideas aportadas para mejorar el trabajo. A la Dra. Rocío Reyes, por aceptar ser parte del comité tutor siendo tutor invitado, así como las ideas y el tiempo invertido de su parte, para la revisión de la tesis. Al Dr. Francisco Arenas, por su tiempo y preocupación para el desarrollo de este trabajo, así como de mi persona. Finalmente a los Doctores que formaron parte del jurado para la presentación de este examen. La Dra. Ma. Hilda Flores Overa, Dra. Ma. Elena Calderón Segura, Dr. Edgar Zenteno, Dr. Ricardo Lascurain Ledesma y el Dr. Alexander Correa-Metrio. Al Departamento de Bioquímica, de la Facultad de Medicina, en especial al Laboratorio 6 por haber aportado todo lo necesario para la realización de la parte inmunológica de este estudio, como material, equipo y experiencia de la Dra. C. Agundis Mata, Dr. E. Zenteno y el Dr. MA. Pereira. Al Museo de Historia Natural (2ª sección del Bosque de Chapultepec), y al equipo que trabaja ahí, en especial el equipo de mantenimiento y vigilancia por todo el apoyo brindado hasta la fecha para la realización de los muestreos, cada semana. Al Programa de Estaciones Meteorológicas de Bachillerato Universitario (PEMBU), por proporcionar los datos meteorológicos de la estación ENP4 Al departamento de Biología Vegetal de la Universidad de Málaga, por el apoyo y los conocimientos aportados para la realización de este estudio, en especial a la Dra. Ma del Mar Trigo Pérez por la orientación palinológica para el inicio de este trabajo. Así como a, la Dra. Marta Recio Criado, la Dra. Silvia Docampo Fernández y el Dr. José García Sánchez. Al equipo de instrumentación del Centro de Ciencias de la Atmósfera, los Ingenieros León Manuel García Espinoza y Wilfrido Gutiérrez López. Al Biólogo Miguel Meneses, por la construcción de la trampa pasiva de esporas, con sus respectivas modificaciones. Este trabajo está dedicado a todas las personas que son importantes en mi vida o en este trabajo, iniciando con mis papás que siempre me han apoyado, ya sea personal o económicamente, porque sin su apoyo, no habría sido posible la realización de este trabajo. A mis hermanas, Maritza y Miriam por estar ahí. A toda mi familia, mi abuela Malena, y a todos mis tíos, que siempre están ahí para apoyarme y obtener distintos logros y dichas. A la Dra Calderón, por el ofrecimiento para iniciar este estudio y ser parte de la creación de la REMA, que nos ha dado mucho trabajo, así como satisfacciones. Además de compartir su tiempo y conocimientos conmigo. Al Dr. Zenteno por todo el apoyo que me brindó para la realización de la parte inmunológica de este estudio y estar siempre dispuesto a discutir los resultados de los experimentos. Al lab. 6 del Depto de Química, la Dra Agundis, el Dr. Alí Pereira, Joserra, José Luis, por ayudarme en los experimentos y hacer divertido trabajar en el lab 6. A todo el grupo del Centro de Ciencias de la Atmósfera, Ma Carmen, Adriana, Fernando, Felipe, César e Ivonn, por formar parte del grupo de trabajo de la REMA y contribuir con esta tesis A Sofía, siempre dispuesta a escuchar y apoyar. Así como a todas las personas que me faltaron por nombrar, pero igual saben que quiero mucho y les agradezco por estar en mi vida. Resumen i Abstract iii Introducción 1 Marco Teórico 2 Aerobiología 2 Proceso aerobiológico de los granos de polen 3 Suspensión y Transporte 5 Resuspensión 6 Depositación e Impactación 6 Morfología Polínica 6 Composición química de los granos de polen 13 Los granos de polen y las alergias 13 Reconocimiento de Alergenos 14 Fra e 1 15 Antecedentes 16 Redes aerobiológicas 17 Calendarios Polínicos 18 Antecedentes Nacionales 20 Aeroalergenos 23 Justificación 25 Objetivo general 26 Objetivos particulares 26 Área de estudio 26 Datos meteorológicos 29 Métodos 30 Obtención del Calendario Polínico 30 Muestreo de granos de polen del la atmósfera del Bosque de 30 Chapultepec con la trampa de esporas tipo Hirst Montaje y tinción de las muestras 31 Conteo e identificación de tipos polínicos en el microscopio óptico a 32 partir de cintas de celofán Análisis de los datos generados durante el muestreo 33 Establecimiento del PPP (Periodo Principal de Polinación) 33 Establecimiento de la VID (Variación Intradiaria) 33 Relación con los datos meteorológicos 33 Formación del Calendario Polínico 37 Muestreo para la detección del aeroalergeno Fra e 1, principal alergeno 37 de Fraxinus spp. Muestreo con la trampa de esporas Ciclón multi-vial 37 Muestreo con la Trampa Pasiva de Esporas 38 Detección Proteica de aeropartículas 39 Extracción de proteínas de granos de polen de Fraxinus spp. obtenidos 39 directamente de la planta. Caracterización del extracto de polen 39 Determinación de proteína 39 Caracterización electroforética del extracto de Fraxinus spp.
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