7KLVLVDSRVWSHHUUHYLHZSUHFRS\HGLWYHUVLRQRI DQDUWLFOHSXEOLVKHGLQ+HUHGLW\7KHILQDO DXWKHQWLFDWHGYHUVLRQLVDYDLODEOHRQOLQHDW KWWSG[GRLRUJV K. Aoki et al. 1 1 Approximate Bayesian computation analysis of EST-associated 2 microsatellites indicates that the broadleaved evergreen tree 3 Castanopsis sieboldii survived the Last Glacial Maximum in 4 multiple refugia in Japan 5 6 K Aoki1, I Tamaki2, K Nakao3, S Ueno4, T Kamijo5, H Setoguchi6, N 7 Murakami7, M Kato6, and Y Tsumura4,5 8 9 1United Graduate School of Child Development, Osaka University, 10 Suita, Osaka 565-0871, Japan, 2Gifu Academy of Forest Science 11 and Culture, Mino, Gifu 501-3714, Japan, 3Kansai Research Center, 12 Forestry and Forest Products Research Institute, Forest Research 13 and Management Organization, Kyoto, Kyoto 612-0855, Japan, 14 4Department of Forest Molecular Genetics and Biotechnology, 15 Forestry and Forest Products Research Institute, Forest Research 16 and Management Organization, Tsukuba, Ibaraki 305-8687, Japan, 17 5Faculty of Life and Environmental Sciences, University of Tsukuba, 18 Tsukuba, Ibaraki 305-8572, Japan, 6Graduate School of Human and 19 Environmental Studies, Kyoto University, Kyoto, Kyoto 606-8501, 20 Japan, 7Makino Herbarium, Tokyo Metropolitan University, Hachioji, 21 Tokyo 192-0397, Japan. 22 K. Aoki et al. 2 23 Correspondence: Saneyoshi Ueno, Department of Forest Molecular 24 Genetics and Biotechnology, Forestry and Forest Products Research 25 Institute, Forest Research and Management Organization, Tsukuba, 26 Ibaraki 305-8687, Japan; Tel. +81 29 829 8261; Fax. +81 29 874 27 3720; E-mail:
[email protected] 28 29 Running title: ABC-based demographic history of Castanopsis 30 K. Aoki et al. 3 31 Abstract 32 Climatic changes have played major roles in plants’ evolutionary 33 history.