Article Identification of a Natural Hybrid between Castanopsis sclerophylla and Castanopsis tibetana (Fagaceae) Based on Chloroplast and Nuclear DNA Sequences Xiaorong Zeng, Risheng Chen, Yunxin Bian, Xinsheng Qin, Zhuoxin Zhang and Ye Sun * Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agriculture University, Guangzhou 510642, China;
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[email protected]; Tel.: +86-136-4265-9676 Received: 30 June 2020; Accepted: 7 August 2020; Published: 11 August 2020 Abstract: Castanopsis kuchugouzhuiHuang et Y. T. Chang was recorded in Flora Reipublicae Popularis × Sinicae (FRPS) as a hybrid species on Yuelushan mountain, but it is treated as a hybrid between Castanopsis sclerophylla (Lindl.) Schott. and Castanopsis tibetana Hance in Flora of China. After a thorough investigation on Yuelushan mountain, we found a population of C. sclerophylla and one individual of C. kuchugouzhui, × but no living individual of C. tibetana. We collected C. kuchugouzhui, and we sampled 42 individuals of × C. sclerophylla from Yuelushan and Xiushui and 43 individuals of C. tibetana from Liangyeshan and Xiushui. Weused chloroplast DNA sequences and 29 nuclear microsatellite markers to investigate if C. kuchugouzhui × is a natural hybrid between C. sclerophylla and C. tibetana. The chloroplast haplotype analysis showed that C. kuchugouzhuishared haplotype H2 with C. sclerophylla on Yuelushan. The STRUCTURE analysis × identified two distinct genetic pools that corresponded well to C. sclerophylla and C. tibetana, revealing the genetic admixture of C.