Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

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Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody Monoclonal Rat IgG2A Clone # 382407 Catalog Number: MAB3454 DESCRIPTION Species Reactivity Mouse Specificity Detects mouse Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In Western blots, no crossreactivity with recombinant human SUMF2 or recombinant mouse SUMF1 is observed. Source Monoclonal Rat IgG2A Clone # 382407 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Mouse myeloma cell line NS0derived recombinant mouse Sulfatase Modifying Factor 2/SUMF2 Gln34Leu308 Accession # Q8BPG6 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Western Blot 1 µg/mL Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2 PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at 20 to 70 °C Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw cycles. l 12 months from date of receipt, 20 to 70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, 20 to 70 °C under sterile conditions after reconstitution. BACKGROUND Sulfatase Modifying Factor 2 (SUMF2) is structurally similar to SUMF1, which activates sulfatases by converting their active site residue cysteine to formylglycine. Functionally, SUMF2 does not activate sulfatases, but rather competes with SUMF1 for the same substrates. The amino acid sequence of mouse SUMF2 is 94%, 83%, 82%, 81% and 72% identical to that of rat, human, canine, chimpanzee and chicken. Rev. 10/13/2015 Page 1 of 1 .

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