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Sandwich ELISAs Unique targets for innovative research 2400 TARGETS 15 SPECIES 1 MANUFACTURER Over 2,400 kits available Pre-coated 96-well strip plates Validated on serum, plasma and media Manufactured in the U.S.A. Over 2,000 publications Risk-free guarantee SPECIES COVERED: Human, mouse, rat, pig, cow, dog, cat, horse, rhesus macaque, rabbit, chicken, dolphin, zebrafish, sigmodon, and sheep 1.888.494.8555 / RAYBIOTECH.COM RayBiotech R Empowering your proteomics SpeedE LISA Need quantitative detection in less time? The RayBio® SpeedELISA is a sandwich ELISA featuring a compressed workflow that allows full processing in only 3 hours. Over 160 kits currently available including human, mouse, and rat targets. Learn more and browse here: www.raybiotech.com/speedelisa-kits 1 1 2 2 3 ADD ANTIBODIES AND CAPTURE ABS BIND TO PLATE; WASH AND SAMPLES TO MICROPLATE ABANTIGEN COMPLEXES FORM DEVELOP COLOR custom ELISA RayBiotech has a vast library of array-validated antibody pairs you can take advantage of, even if they aren’t currently developed on the 96-well platform. Any antibody pair can be developed into a finished ELISA kit by placing an order for a “custom ELISA.” After a 5-7 week development phase, the final kit will have passed our ELISA quality control tests and be added to our stock kit inventory. The development phase only needs to occur once. If the ELISA fails development, the customer will not be charged anything. Need a custom kit for a target we don’t have a pair to? Need 384-well format? Chemiluminescence-based detection? We can do it! Immunoassay development is RayBiotech’s specialty. Let us tailor an ELISA to your specifications, from antibodies to the final kit. Contact [email protected] for more information and a quote. services Send Us Your Samples, We’ll Send You Results RayBiotech offers full testing services in our GLP-compliant laboratory for all sandwich ELISA kits. Receive reports with data of the highest accuracy and reproducibility. Visit www.raybiotech.com/elisa-testing-service/ for more details. how it works 1 MICROPLATE PRECOATED Why RayBiotech ELISAs? WITH CAPTURE ANTIBODY 2 Made here. By us. For you. RayBiotech is the primary manufacturer of validated ELISA kits, not a reseller. This means not ADD SAMPLE OR STANDARD only can we offer you the best price but also prompt and effective product support. B B Commitment to Quality Our mission is to provide you with reliable assays and reagents, period. Every kit we produce 3 is validated, subjected to a battery of rigorous product-specific quality control tests, and manufactured in compliance with GMP and ISO 13485 standards. ADD BIOTINLABELED DETECTION ANTIBODY Expertise and Experience Creating a consistent and reliable ELISA is no easy task. Immunoassays are the core expertise B B and the focus of our large team of experienced R&D scientists. They meticulously test and optimize antibodies, antigens and buffer systems to develop high-performing ELISAs and corresponding antibody arrays. Their dedicated, collaborative efforts have given us the largest 4 selection of fully validated ELISA kits on the market, with over 2000 unique citations in the scientific literature. ADD HRPCONJUGATED STREPTAVIDIN We Support You If you have questions, concerns or feedback, we’re here to help. Our team of technical support B B specialists are fully qualified and trained on every product line and receive consistently high service ratings. Whether you need to troubleshoot your data or want some advice on how to best design your study, we can provide free consultative services to help you achieve success 5 in your research. Reach out by phone email, or live chat today! ADD COLORIMETRIC We Appreciate You SUBSTRATE RayBiotech’s customer reward program includes valuable discounts and incentives for purchases, publications and product reviews. It’s our way of letting you know we value you and your research efforts. Risk-free Guarantee We understand the risk of trying a new assay with no guarantee of success. That’s why our Specific capture antibodies are coated ELISA kits are covered by a 100% guarantee – so that you can evaluate one of our kits with onto a 96-well plate. Standards and no risks, no worries. If you encounter a problem using one of our ELISA kits and our technical samples are pipetted into the wells; support team cannot satisfactorily resolve it, we will issue you a replacement, credit or refund. the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. human targets BMP-6 CILP-1 ErbB4 GPV IL-18 R beta Lipocalin-2 # BMP-7 CK-MB Erythropoietin Granzyme A IL-2 Livin 15-PGDH BMP-8 CLEC-2 Erythropoietin R Granzyme B IL-2 R alpha LOX-1 2B4 BMP-9 Clusterin ESAM GRO alpha IL-2 R beta Lp-PLA2 4-1BB BMPR-II c-Myc E-Selectin GRO alpha/beta/gamma IL-20 LRG1 4-1BB Ligand BNP CNTF Growth Hormone IL-21 L-Selectin 6Ckine Brevican CNTF R alpha Growth Hormone R IL-21 R LTA4H Coagulation Factor III F GSTM1 IL-22 Lumican Coagulation Factor VII FABP2 GSTP1 IL-22 R alpha 1 Luteinizing hormone A FABP3 Activin A C Coagulation Factor XI IL-22BP Lymphotactin C1q R1 FABP4 Activin C Coagulation Factor XIV IL-23 LYVE-1 C1qTNF1 FABP5 ADA COCO H IL-23 R C1qTNF5 FABP6 HAI-1 ADAM-12 Complement Factor H IL-24 C1qTNF9 FABP8 HAI-2 ADAM-9 Complement MASP3 IL-26 M C1R FAP HB-EGF MAGP-2 ADAMTS-4 Contactin-1 IL-27 C1S Fas HCC-1 MAPT Adiponectin Corneodesmosin IL-28A C3a Fas Ligand HCC-4 Marapsin Adipsin COX-2 IL-29 C4c Fc gamma RIIB/C hCG beta Matrilin-2 aFGF C-Peptide IL-3 C5a FCRL3 hCG intact Matrilin-3 Aggrecan CRELD2 IL-31 CA125 FCRL5 Hemoglobin Matriptase AgRP CRIM1 IL-32 alpha CA13 Ferritin Hemopexin MBL Albumin CrkL IL-33 CA15-3 Fetuin A Hepassocin MCAM ALCAM CRP IL-34 CA19-9 Fetuin B Hepsin MCP-1 ALDH1A1 CRTAC1 IL-36 alpha CA9 FGF-12 HEXA MCP-2 ALK-6 CRTAM IL-36 gamma Cadherin-11 FGF-16 HGF MCP-3 Alkaline Phosphatase CTACK IL-36 Ra Cadherin-13 FGF-17 HGFR MCP-4 Alpha-2 Macroglobulin CXCL14 IL-37 Calcitonin FGF-19 HIF-1 alpha M-CSF Alpha-fetoprotein CXCL16 IL-4 Calreticulin FGF-20 HPRG MD-2 AMICA CXCL17 I-309 Carboxylesterase 1 FGF-21 HSP27 MDC AmiGO2 Cyclophilin A ICAM-1 Carboxylesterase 2 FGF-23 HSP60 Mer Aminopeptidase LRAP Cyr61 ICAM-3 Carboxypeptidase A1 FGF-4 HSP70 Mesothelin Aminopeptidase N Cystatin C ICOS Carboxypeptidase B1 FGF-5 HTRA2 METAP2 Aminopeptidase P1 Cystatin D IL-5 Carboxypeptidase B2 FGF-6 HVEM MFG-E8 Amnionless Cystatin SN IL-5 R alpha Cardiac Troponin I FGF-7 MICA Amphiregulin Cytokeratin 18 IL-6 Cardiotrophin-1 FGF-9 MICB Amyloid beta 1-40 Cytokeratin 19 IL-6 R Carnosine Dipeptidase-1 Fibronectin I Midkine Angiogenin Cytokeratin 8 IL-7 Caspase-3 Ficolin-2 IDO MIF Angiopoietin-1 IL-7 R alpha Caspase-7 FKBP51 IDUA MIG Angiopoietin-2 IL-8 Cathepsin C FLRG IFN-alpha MIP-1 alpha Angiopoietin-4 D IL-9 Cathepsin D DAN FLRT2 IFN-alpha/beta R2 MIP-1 beta Angiostatin Importin alpha 2 Cathepsin L DCBLD2 Flt-3 IFN-gamma MIP-1 delta Angiotensinogen Inhibin A Cathepsin S DcR3 Flt-3 Ligand IFN-gamma R1 MIP-3 alpha ANGPTL3 Insulin CCL28 DC-SIGNR Follistatin IGF-1 MIP-3 beta ANGPTL4 Integrin alpha-2 CD14 D-Dimer FOLR1 IGF-1 R MMP-1 ANGPTL7 Integrin alpha-M CD155 Decorin FOLR3 IGF-2 R MMP-10 Annexin A1 IP-10 CD163 Dectin-1 Fractalkine IGFBP-1 MMP-12 ApoA1 Islet-1 CD177 Desmin FSH IGFBP-2 MMP-13 ApoA2 I-TAC CD200 Desmocollin-3 Furin IGFBP-3 MMP-2 ApoB CD229 Desmoglein-1 IGFBP-4 MMP-3 ApoB100 CD23 Desmoglein-2 IGFBP-5 MMP-7 ApoC1 J CD26 DKK-1 G IGFBP-6 Jagged 1 MMP-8 ApoC2 CD27 Ligand DKK-3 Galectin-1 IGFBP-rp1 JAM-A MMP-9 ApoC3 CD30 DKK-4 Galectin-3 IgG JAM-B MMR ApoE CD30 Ligand DLEC Galectin-7 IgG1 JAM-C MPIF-1 ApoE2 CD34 DLL1 Galectin-8 IL-1 alpha MSP alpha/beta ApoE3 CD35 DMP-1 Galectin-9 IL-1 beta Myoglobin ApoE4 CD36 DNAM-1 GAPHD IL-1 R1 K ApoH CD38 DPPII Gas 1 IL-1 R2 Kallikrein 10 Arginase 1 CD4 Draxin Gas 6 IL-1 R4 Kallikrein 14 N Artemin CD40 DSCAM-L1 GASP-1 IL-1 R6 Kallikrein 6 NAP-2 AXL CD40 Ligand Dtk GATA-1 IL-1 Ra Kininogen NCAM-1 CD42b GCP-2 IL-10 KIRREL3 Nectin-2 CD51 GCSF IL-10 R alpha Kynureninase NELL1 B GCSF R IL-10 R beta Neprilysin B7-H1 CD59 E ECM-1 GDF-11 IL-11 Nesfatin-1 B7-H2 CD69 EDA-A2 GDF-15 IL-12 p40 L Nestin BACE-1 CD74 EGF GDF-8 IL-12 p70 L1CAM Neurocan BCAM CD80 EGFR GDNF IL-13 Lactoferrin Neuropilin-2 bcl-w CD86 EGR1 GFR alpha-3 IL-13 R alpha 1 LAG-3 NG2 BCMA CD99 EG-VEGF Ghrelin IL-13 R alpha 2 LAIR1 NGFR BDNF CDNF ENA-78 GITR IL-15 Laminin alpha 4 Nidogen-1 beta IG-H3 CEA Endoglin GITR Ligand IL-15 R alpha LAMP1 Nidogen-2 Beta-2 Microglobulin CEACAM-1 Endostatin GLI-3 IL-16 LAMP2 NMNAT-1 Betacellulin Cerberus 1 Endothelin-1 GLP-1 IL-17 RA LBP Nogo-A beta-NGF CFHR1 eNOS Glucagon IL-17 RC LDL R Norrin bFGF CFHR5 EN-RAGE Glypican 1 IL-17A Lefty-A Notch-1 Biglycan Chemerin Eotaxin-1 Glypican 3 IL-17B Legumain Notch-3 BLC CHI3L1 Eotaxin-2 GM-CSF IL-17E Leptin NOV BMP-15 Chitotriosidase Eotaxin-3 GM-CSF R alpha IL-17F LIF NPTXR BMP-2 Chordin-Like-1 EphA2 GOLM1 IL-18 LIGHT Nr-CAM BMP-4 CHST4 ErbB2 gp130 IL-18 BP alpha LILRB4 NRF2 BMP-5 cIAP-1 cIAP-2 ErbB3 GPR56 IL-18 R alpha LIMPII NRG1-alpha NRG1-beta 1 PECAM-1 RANTES SERPIN G1 TECK TRAIL R4 VEGF-B NT-3 Pentraxin-3 RBP4 sFRP-1 Tenascin C Transferrin VEGF-C NT-4 Pepsinogen 1 Reg1B sFRP-3 Tenascin R Transglutaminase 2 VEGF-D NTB-A Pepsinogen 2 Renin SHBG TFF3 Transglutaminase 3 VEGFR1 Periostin Resistin ShhN TFPI Trappin-2 VEGFR2 PGRP-S Ret SIGIRR
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    www.nature.com/scientificreports OPEN Disease‑related cellular protein networks diferentially afected under diferent EGFR mutations in lung adenocarcinoma Toshihide Nishimura1,8*, Haruhiko Nakamura1,2,8, Ayako Yachie3,8, Takeshi Hase3,8, Kiyonaga Fujii1,8, Hirotaka Koizumi4, Saeko Naruki4, Masayuki Takagi4, Yukiko Matsuoka3, Naoki Furuya5, Harubumi Kato6,7 & Hisashi Saji2 It is unclear how epidermal growth factor receptor EGFR major driver mutations (L858R or Ex19del) afect downstream molecular networks and pathways. This study aimed to provide information on the infuences of these mutations. The study assessed 36 protein expression profles of lung adenocarcinoma (Ex19del, nine; L858R, nine; no Ex19del/L858R, 18). Weighted gene co-expression network analysis together with analysis of variance-based screening identifed 13 co-expressed modules and their eigen proteins. Pathway enrichment analysis for the Ex19del mutation demonstrated involvement of SUMOylation, epithelial and mesenchymal transition, ERK/mitogen- activated protein kinase signalling via phosphorylation and Hippo signalling. Additionally, analysis for the L858R mutation identifed various pathways related to cancer cell survival and death. With regard to the Ex19del mutation, ROCK, RPS6KA1, ARF1, IL2RA and several ErbB pathways were upregulated, whereas AURK and GSKIP were downregulated. With regard to the L858R mutation, RB1, TSC22D3 and DOCK1 were downregulated, whereas various networks, including VEGFA, were moderately upregulated. In all mutation types, CD80/CD86 (B7), MHC, CIITA and IFGN were activated, whereas CD37 and SAFB were inhibited. Costimulatory immune-checkpoint pathways by B7/CD28 were mainly activated, whereas those by PD-1/PD-L1 were inhibited. Our fndings may help identify potential therapeutic targets and develop therapeutic strategies to improve patient outcomes.
  • Exploration of Prognostic Biomarkers and Therapeutic Targets in the Microenvironment of Bladder Cancer Based on CXC Chemokines

    Exploration of Prognostic Biomarkers and Therapeutic Targets in the Microenvironment of Bladder Cancer Based on CXC Chemokines

    Exploration of Prognostic Biomarkers and Therapeutic Targets in The Microenvironment of Bladder Cancer Based on CXC Chemokines Xiaoqi Sun Department of Urology, Kaiping Central Hospital, Kaiping, 529300, China Qunxi Chen Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China Lihong Zhang Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China Jiewei Chen Department of Pathology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China Xinke Zhang ( [email protected] ) Sun Yat-sen University Cancer Center Research Keywords: Bladder cancer, Biomarkers, CXC Chemokines, Microenvironment Posted Date: February 24th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-223127/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/29 Abstract Background: Bladder cancer (BLCA) has a high rate of morbidity and mortality, and is considered as one of the most malignant tumors of the urinary system. Tumor cells interact with surrounding interstitial cells, playing a key role in carcinogenesis and progression, which is partly mediated by chemokines. CXC chemokines exert anti‐tumor biological roles in the tumor microenvironment and affect patient prognosis. Nevertheless, their expression and prognostic values patients with BLCA remain unclear. Methods: We used online tools, including Oncomine, UALCAN, GEPIA, GEO databases, cBioPortal, GeneMANIA, DAVID 6.8, Metascape, TRUST (version 2.0), LinkedOmics, TCGA, and TIMER2.0 to perform the relevant analysis. Results: The mRNA levels of C-X-C motif chemokine ligand (CXCL)1, CXCL5, CXCL6, CXCL7, CXCL9, CXCL10, CXCL11, CXCL13, CXCL16, and CXCL17 were increased signicantly increased, and those of CXCL2, CXCL3, and CXCL12 were decreased signicantly in BLCA tissues as assessed using the Oncomine, TCGA, and GEO databases.
  • Article Characterization of HMGB1/2 Interactome in Prostate Cancer by Yeast Two Hybrid Approach: Potential Pathobiological Implications

    Article Characterization of HMGB1/2 Interactome in Prostate Cancer by Yeast Two Hybrid Approach: Potential Pathobiological Implications

    Article Characterization of HMGB1/2 Interactome in Prostate Cancer by Yeast Two Hybrid Approach: Potential Pathobiological Implications Aida Barreiro-Alonso 1,†, María Cámara-Quílez 1,†, Martín Salamini-Montemurri 1, Mónica Lamas- Maceiras 1, Ángel Vizoso-Vázquez 1, Esther Rodríguez-Belmonte 1, María Quindós-Varela 2, Olaia Martínez-Iglesias 3, Angélica Figueroa 3 and María-Esperanza Cerdán 1,* Table S1. Association of proteins that interact with Hmgb1 or Hmgb2 to cancer hallmarks. Cancer Hallmark Protein Model Reference MAP1B Colorectal cancers [109] GENOMIC INSTABILITY AND Liver adenocarcinoma (SKHep1) and NOP53 [110] MUTATIONS/ CHANGES IN glioblastoma (T98G) cell lines TELOMERASE ACTIVITY RSF1 Ovarian cells [111] SRSF3 Ovarian cancer [112] C1QBP Breast cancer [54,113] cFOS Bladder cancer [114] cFOS Breast cancer [115] DLAT Gastric Cancer [116] Human melanoma (A7), Prostate cancer FLNA [117] (PC3) cell lines GOLM1 Hepatocellular carcinoma [118] GOLM1 Breast cancer [119] GOLM1 Lung proliferation [120] GOLM1 Prostate cancer [82] SUSTAINING PROLIFERATIVE Hox-A10 Prostate cancer cell line PC-3 [58] SIGNALLING/ CELL Hox-A10 Ovarian cancer cell lines [121,122] PROLIFERATION NOP53 Breast cancer [123] PSMA7 Cervical cancer [124] PTPN2 Epithelial carcinogenesis [125] RASAL2 hepatocellular carcinoma [126] RSF1 Prostate cancer [95] RSF1 Nasopharyngeal carcinoma [127] SPIN1 Glioma [128] TGM3 Esophageal cancer [129] UBE2E3 Retinal pigment epithelial (RPE) [130] Vigilin Hepatocellular carcinomas [131] WNK4 Kidney [100] C1QBP Prostate cancer [48]