Abstracts of Scientific Presentations 2019 AALAS National Meeting Denver, Colorado
Total Page：16
File Type：pdf, Size：1020Kb
Journal of the American Association for Laboratory Animal Science Vol 58, No 5 Copyright 2019 September 2019 by the American Association for Laboratory Animal Science Pages 607–726 Abstracts of Scientific Presentations 2019 AALAS National Meeting Denver, Colorado Poster Sessions group each of NSG and NSG-SGM3 mice were microchipped using a physical restraint device, and 2 groups of NSG-SGM3 mice were P1 People Advocating Wellness and Support: Compassion Fatigue microchipped under isoflurane anesthesia. One anesthetic group and Satisfaction Team was microchipped with a surgical skin preparation and the other was not. Mice were monitored for weight loss, signs of infection, A Schoell* and complications, such as inflammation, barbering, or microchip loss. After 2-8 wk, mice were euthanized for gross pathology, Zoetis, Kalamazoo, MI subcutaneous culture, and histology. The restraint device method was then similarly validated for groups (n=20) of nude (J:NU) and People working in laboratory animal research are exposed to a humanized (NSG and NSG-SGM3) mice. Pathological assessment variety of situations and experiences that, over time, can cause did not reveal a significant difference in inflammation (P > 0.999) chronic stress and emotional exhaustion. These situations and between groups, and all culture results were negative. Weights experiences include frequent euthanasia of animals, creation and postimplantation did not vary significantly between groups (P > maintenance of animal disease models, unexpected adverse events 0.06). The only noteworthy complication was occasional microchip involving animals, and many more. These experiences can negatively loss immediately following implantation using the physical restraint affect a person and lead to compassion fatigue. Compassion fatigue is device, with no persisting negative effects. We concluded that all a form of secondary traumatic stress that can be experienced by those of the assessed microchipping methods were acceptable for use on helping people or animals in distress. To help support our staff who immunocompromised mice. may experience varying degrees of compassion fatigue, we formed an internal team dedicated to addressing compassion fatigue and P3 Dominance Status Has No Effect on Data Produced by SIV- improving compassion satisfaction. The name of the team is People infected Macaque (Macaca mulatta) Model and Remains Constant Advocating Wellness and Support (PAWS). The mission is to be a over Course of Infection cross-function team to raise awareness, educate, and stimulate open conversation about compassion fatigue and compassion satisfaction A McNamara*, B Carlson, S Flemming, EK Hutchinson, M Li, S within our organization. The team will work to support and identify Queen, E Shirk, R Adams, J Clements, J Mankowski, KA Metcalf Pate needs within our organization that can positively influence and support those people experiencing compassion fatigue. To help Molecular and Comparative Pathobiology, Johns Hopkins University, steer the direction of the team and identify needs of our people, Baltimore, MD we conducted a needs assessment in partnership with an external consultant. Our team has used the needs assessment to launch Establishment of a dominance hierarchy is a natural consequence of initiatives that help focus on our core ACE principle: awareness, socially housing macaques. Though social housing has been shown communication, and education around compassion fatigue and to improve welfare, reduce stress, and improve research within compassion satisfaction. In the long term, we hope to positively the SIV-infected macaque model of HIV infection, it is unclear if support and improve the lives of our employees through PAWS. dominance status has an influence on SIV infection and subsequently could introduce variability into the data produced by the model. P2 Assessment of Microchip Placement Methods in We sought to determine if dominance status within a pair has an Immunocompromised Mice effect on the immunological parameters of SIV infection, and if the dominance status of a given macaque remains constant throughout A Hernandez*1, P Torres2, P Collier2, M Campagna1 the course of infection. We hypothesized that dominance status may affect immunological parameters. In addition, we hypothesized that 1Comparative Medicine and Quality, The Jackson Laboratory, dominance would remain unchanged from preinoculation through Sacramento, CA; 2The Jackson Laboratory, Sacramento, CA acute infection and suppression. We determined dominance status prior to inoculation for 12 socially housed Rhesus macaques (Macaca Microchipping has become an increasingly popular means of mulatta) using an ethogram to score incidents of dominant behavior identification in laboratory mice. Compared to other identification at feeding time. Following inoculation with SIV, we repeated methods, such as ear tags, microchips are less likely to cause dominance scoring during acute infection and following viral irritation, fall out, or be misread. Microchipped mice can easily be suppression. We determined plasma and CSF viral loads using qRT- scanned, and any associated data can be linked directly to their PCR, CD4 T cell counts by flow cytometry, and platelet counts via a microchip numbers. General anesthesia is often considered to be complete blood count throughout infection. All data was normalized the most controlled method of restraint for microchip implantation, to each individual macaque’s baseline data and analyzed. There was but other options exist. In our facility, physical restraint devices no significant difference between dominant and subordinate animals are commonly used without complication in immunocompetent in plasma and CSF viral loads, CD4 T cell counts, or platelet counts. mice. However, special considerations are often needed when Furthermore, dominance status remained unchanged throughout translating methods to immunocompromised animals, such as usage SIV infection for all pairs. These results demonstrate that dominance of more stringent practices. Therefore, a study was conducted to status does not interfere with or alter the course of infection and assess complication rates in immunocompromised mice implanted that dominance status remains constant throughout infection. Thus, with microchips using either physical or chemical restraint, and dominance hierarchy is unlikely to affect data produced by the SIV- with or without surgical skin preparation. We hypothesized that infected macaque model, and concerns about increasing variability complication rates would be similar between these different of the data should not deter researchers from socially housing techniques. NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) and NSG-SGM3 macaques within the model. [NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/ MloySzJ] mice were divided into 4 groups (n=20 per group). One 607 Vol 58, No 5 Journal of the American Association for Laboratory Animal Science September 2019 P4 Optimization of Intrathecal Administration in Rats Using approach for “sticky” tracers that have a higher residual when other Fluoroscopic Guidance tubing or needle and syringe set ups are used; and a butterfly with saline and tongue depressor approach for obtaining and maintaining A Jones*, G Sweet, R Pielemeier venous access for anesthetized animals. When comparing these to traditional injection techniques, we have found less perivascular Surgery and Efficacy, Charles River Labs, Kalamazoo, MI infiltration, better image quality, less loss of tracers and radioactivity, and higher levels of proficiency by technicians Neurodegenerative diseases affect millions of people in the U.S. and the incidence continues to increase. Intrathecal injection (the P6 Optimizing Success in Nonhuman Primate Work with intended route in humans) can be used to target the central nervous Individualized Problem-solving Strategies system (CNS), providing analgesia, chemotherapy treatment, as well as gene and antibiotic therapy. Intrathecal administration allows AR Knight*, K Phataraphruk for smaller doses, avoids the need to cross the blood-brain barrier and may result in fewer systemic side effects. Potential efficacy School of Biological and Health Systems Engineering, Arizona State and safety of intrathecal administration test articles are frequently University, Tempe, AZ tested in rats, most typically done by unguided injection, surgical procedure, catheter, or port. Our facility has developed a method of Current training and restraint protocols for rhesus macaques (Macaca direct percutaneous intrathecal injection using fluoroscopic guidance. mulatta) typically involve generic solutions to behavioral challenges. To perform this method, the rats are anesthetized to effect with This 1-size-fits-all approach, while largely effective, still leaves room isoflurane. The animals are then placed in a ventral recumbency. for improvement. Working with nonhuman primates presents a A 27 g Lasse needle and a 100 µL gas tight syringe are filled with plethora of challenges that may benefit from a more individualized the test material. Using aseptic technique, the lumbar cistern is approach, particularly when an animal exhibits unique behaviors or accessed percutaneously under fluoroscopic guidance. Once the learning capabilities. Modifications to our current training and needle is in place, the test material is injected by slow bolus. The restraint protocols were inspired by 1 rhesus macaque that exhibited needle is then removed, and the animal recovered. To determine