RESEARCH ARTICLE Genome Anatomy of Pyrenochaeta unguis- hominis UM 256, a Multidrug Resistant Strain Isolated from Skin Scraping Yue Fen Toh1, Su Mei Yew1, Chai Ling Chan1, Shiang Ling Na1, Kok Wei Lee2, Chee- Choong Hoh2, Wai-Yan Yee2, Kee Peng Ng1, Chee Sian Kuan1* 1 Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, 2 Codon Genomics SB, Seri Kembangan, Selangor Darul Ehsan, Malaysia *
[email protected] a11111 Abstract Pyrenochaeta unguis-hominis is a rare human pathogen that causes infection in human skin and nail. P. unguis-hominis has received little attention, and thus, the basic biology and pathogenicity of this fungus is not fully understood. In this study, we performed in-depth OPEN ACCESS analysis of the P. unguis-hominis UM 256 genome that was isolated from the skin scraping Citation: Toh YF, Yew SM, Chan CL, Na SL, Lee of a dermatitis patient. The isolate was identified to species level using a comprehensive KW, Hoh C-C, et al. (2016) Genome Anatomy of Pyrenochaeta unguis-hominis UM 256, a Multidrug multilocus phylogenetic analysis of the genus Pyrenochaeta. The assembled UM 256 Resistant Strain Isolated from Skin Scraping. PLoS genome has a size of 35.5 Mb and encodes 12,545 putative genes, and 0.34% of the ONE 11(9): e0162095. doi:10.1371/journal. assembled genome is predicted transposable elements. Its genomic features propose that pone.0162095 the fungus is a heterothallic fungus that encodes a wide array of plant cell wall degrading Editor: Joy Sturtevant, Louisiana State University, enzymes, peptidases, and secondary metabolite biosynthetic enzymes.