COX5B Antibody Cat. No.: 18-748
COX5B Antibody
Immunohistochemistry of paraffin-embedded Immunohistochemistry of paraffin-embedded mouse heart using human liver damage using COX5B antibody COX5B antibody (18-748) at dilution of 1:100 (40x lens). (18-748) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells Immunofluorescence analysis of C6 cells using COX5B antibody using COX5B antibody (18-748) at dilution of 1:100. (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining. Blue: DAPI for nuclear staining.
September 29, 2021 1 https://www.prosci-inc.com/cox5b-antibody-18-748.html Immunoprecipitation analysis of 200ug extracts of Immunofluorescence analysis of U-2 OS cells using COX5B HepG2 cells, using 3 ug COX5B antibody (18-748). antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear Western blot was performed from the staining. immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 IMMUNOGEN: of human COX5B (NP_001853.2).
TESTED APPLICATIONS: IF, IHC, IP, WB
WB: ,1:500 - 1:2000
IHC: ,1:50 - 1:100 APPLICATIONS: IF: ,1:50 - 1:100
IP: ,1:50 - 1:100
POSITIVE CONTROL: 1) HepG2
2) 22Rv1
3) SW480
4) HeLa
5) A-549
6) Mouse heart
PREDICTED MOLECULAR Observed: 14kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
September 29, 2021 2 https://www.prosci-inc.com/cox5b-antibody-18-748.html ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: COX5B
Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, ALTERNATE NAMES: COX5B
GENE ID: 1329
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and BACKGROUND: nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 29, 2021 3 https://www.prosci-inc.com/cox5b-antibody-18-748.html