COX5B Antibody Cat. No.: 18-748

COX5B Antibody

Immunohistochemistry of paraffin-embedded Immunohistochemistry of paraffin-embedded mouse heart using human liver damage using COX5B antibody COX5B antibody (18-748) at dilution of 1:100 (40x lens). (18-748) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells Immunofluorescence analysis of C6 cells using COX5B antibody using COX5B antibody (18-748) at dilution of 1:100. (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining. Blue: DAPI for nuclear staining.

September 29, 2021 1 https://www.prosci-inc.com/cox5b-antibody-18-748.html Immunoprecipitation analysis of 200ug extracts of Immunofluorescence analysis of U-2 OS cells using COX5B HepG2 cells, using 3 ug COX5B antibody (18-748). antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear Western blot was performed from the staining. immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 IMMUNOGEN: of human COX5B (NP_001853.2).

TESTED APPLICATIONS: IF, IHC, IP, WB

WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:100 APPLICATIONS: IF: ,1:50 - 1:100

IP: ,1:50 - 1:100

POSITIVE CONTROL: 1) HepG2

2) 22Rv1

3) SW480

4) HeLa

5) A-549

6) Mouse heart

PREDICTED MOLECULAR Observed: 14kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

September 29, 2021 2 https://www.prosci-inc.com/cox5b-antibody-18-748.html ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: COX5B

Cytochrome c oxidase subunit 5B, mitochondrial, polypeptide Vb, ALTERNATE NAMES: COX5B

GENE ID: 1329

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Cytochrome C oxidase (COX) is the terminal of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and BACKGROUND: nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 29, 2021 3 https://www.prosci-inc.com/cox5b-antibody-18-748.html