COX5B Antibody Cat. No.: 18-748 COX5B Antibody Immunohistochemistry of paraffin-embedded Immunohistochemistry of paraffin-embedded mouse heart using human liver damage using COX5B antibody COX5B antibody (18-748) at dilution of 1:100 (40x lens). (18-748) at dilution of 1:100 (40x lens). Immunofluorescence analysis of NIH-3T3 cells Immunofluorescence analysis of C6 cells using COX5B antibody using COX5B antibody (18-748) at dilution of 1:100. (18-748) at dilution of 1:100. Blue: DAPI for nuclear staining. Blue: DAPI for nuclear staining. September 29, 2021 1 https://www.prosci-inc.com/cox5b-antibody-18-748.html Immunoprecipitation analysis of 200ug extracts of Immunofluorescence analysis of U-2 OS cells using COX5B HepG2 cells, using 3 ug COX5B antibody (18-748). antibody (18-748) at dilution of 1:100. Blue: DAPI for nuclear Western blot was performed from the staining. immunoprecipitate using COX5B antibody (18-748) at a dilition of 1:1000. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 IMMUNOGEN: of human COX5B (NP_001853.2). TESTED APPLICATIONS: IF, IHC, IP, WB WB: ,1:500 - 1:2000 IHC: ,1:50 - 1:100 APPLICATIONS: IF: ,1:50 - 1:100 IP: ,1:50 - 1:100 POSITIVE CONTROL: 1) HepG2 2) 22Rv1 3) SW480 4) HeLa 5) A-549 6) Mouse heart PREDICTED MOLECULAR Observed: 14kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal September 29, 2021 2 https://www.prosci-inc.com/cox5b-antibody-18-748.html ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: COX5B Cytochrome c oxidase subunit 5B, mitochondrial, Cytochrome c oxidase polypeptide Vb, ALTERNATE NAMES: COX5B GENE ID: 1329 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and BACKGROUND: nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 3 https://www.prosci-inc.com/cox5b-antibody-18-748.html.