Molecular Systematics of Orchids
American Journal of Botany 86(2): 208±224. 1999. A PHYLOGENETIC ANALYSIS OF THE ORCHIDACEAE: EVIDENCE FROM RBCL NUCLEOTIDE SEQUENCES1 KENNETH M. CAMERON,2,9 MARK W. C HASE,3 W. M ARK WHITTEN,4 PAUL J. KORES,3,10 DAVID C. JARRELL,5 VICTOR A. ALBERT,2 TOMOHISA YUKAWA,6 HAROLD G. HILLS,7 AND DOUGLAS H. GOLDMAN8 2The Lewis B. and Dorothy Cullman Program for Molecular Systematics Studies, The New York Botanical Garden, Bronx, New York 10458-5126; 3Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, United Kingdom; 4Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611-2009; 5Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824-1312; 6Tsukuba Botanical Garden, National Science Museum, 4-1-1 Amakubo, Tsukuba, Ibaraki 305, Japan; 7DNA Sequencing Facility, Iowa State University, Ames, Iowa 50014-3260; and 8Department of Botany, University of Texas, Austin, Texas 78712 Cladistic parsimony analyses of rbcL nucleotide sequence data from 171 taxa representing nearly all tribes and subtribes of Orchidaceae are presented here. These analyses divide the family into ®ve primary monophyletic clades: apostasioid, cypripedioid, vanilloid, orchidoid, and epidendroid orchids, arranged in that order. These clades, with the exception of the vanilloids, essentially correspond to currently recognized subfamilies. A distinct subfamily, based upon tribe Vanilleae, is supported for Vanilla and its allies. The general tree topology is, for the most part, congruent with previously published hypotheses of intrafamilial relationships; however, there is no evidence supporting the previously recognized subfamilies Spiranthoideae, Neottioideae, or Vandoideae. Subfamily Spiranthoideae is embedded within a single clade containing mem- bers of Orchidoideae and sister to tribe Diurideae.
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