(12) United States Patent (10) Patent No.: US 7,955,813 B2 De Kreijet Al

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(12) United States Patent (10) Patent No.: US 7,955,813 B2 De Kreijet Al US007955813B2 (12) United States Patent (10) Patent No.: US 7,955,813 B2 De Kreijet al. (45) Date of Patent: *Jun. 7, 2011 (54) METHOD OF USING LIPID 3,939,350 A 2f1976 Kronicket al. ACYLTRANSFERASE 3,973,042 A 8, 1976 Kosikowski 3.996,345 A 12/1976 Ullman et al. 4,034,124 A 7, 1977 Van Dam (75) Inventors: Arno De Kreij, Papendrecht (NL); 4,065,580 A 12/1977 Feldman Susan Mampusti Madrid, Vedbaek 4,160,848 A T. 1979 Vidal 4,202,941 A 5/1980 Terada (DK); Jorn Dalgaard Mikkelsen, 4,275,149 A 6/1981 Litman et al. Hvidovre (DK); Jorn Borch Soe, Tilst 4,277.437 A 7/1981 Maggio (DK) 4,366,241 A 12/1982 Tom et al. 4,399.218 A 8, 1983 Gauhl (73) Assignee: Danisco, A/S, Copenhagen (DK) 4,567.046 A 1/1986 Inoue 4,683.202 A 7, 1987 Mullis 4,689,297 A 8, 1987 Good (*) Notice: Subject to any disclaimer, the term of this 4,707,291 A 11, 1987 Thom patent is extended or adjusted under 35 4,707,364 A 11/1987 Barach U.S.C. 154(b) by 1056 days. 4,708,876 A 1 1/1987 Yokoyama 4,798,793 A 1/1989 Eigtved This patent is Subject to a terminal dis 4,808,417 A 2f1989 Masuda claimer. 4,810,414 A 3/1989 Huge-Jensen 4,814,331 A 3, 1989 Kerkenaar 4,816,567 A 3/1989 Cabilly et al. (21) Appl. No.: 11/483,345 4,818,695 A 4/1989 Eigtved 4,826,767 A 5/1989 Hansen (22) Filed: Jul. 7, 2006 4,865,866 A 9, 1989 Moore 4,904.483. A 2f1990 Christensen (65) Prior Publication Data 4,916,064 A 4, 1990 Derez 5,112,624 A 5/1992 Johna US 2007/0026106A1 Feb. 1, 2007 5,213,968 A 5, 1993 Castle 5,219,733 A 6/1993 Myojo 5,219,744 A 6/1993 Kurashige Related U.S. Application Data 5,232,846 A 8, 1993 Takeda (63) Continuation of application No. 1 1/182,408, filed on 5,264,367 A 11/1993 Aalrust 5,273,898 A 12, 1993 Ishii Jul. 15, 2005, now Pat. No. 7,807,398, which is a 5,288,619 A 2f1994 Brown continuation-in-part of application No. 5,290,694 A 3, 1994 Nakanishi PCT/IB2004/000655, filed on Jan. 15, 2004. 5,310,679 A 5/1994 Artiss et al. (60) Provisional application No. 60/489.441, filed on Jul. (Continued) 23, 2003. FOREIGN PATENT DOCUMENTS (30) Foreign Application Priority Data AR 331094 2, 1995 (Continued) Jan. 17, 2003 (GB) ................................... O3O1117.8 Jan. 17, 2003 (GB) ...... ... O3O1118.6 OTHER PUBLICATIONS Jan. 17, 2003 (GB) ...... ... O301 1194 Jan. 17, 2003 (GB) ...... ... O3O112O2 Fernandez-Garcia et al. J. Dairy Sci. 77: 2139-2149 (1994).* Jan. 17, 2003 (GB) ...... ... O3O1121.0 Jan. 17, 2003 (GB) ...... ... O3O1122.8 (Continued) Dec. 24, 2003 (GB) ................................... O33OO16.7 Primary Examiner — Tekchand Saidha (51) Int. Cl. (74) Attorney, Agent, or Firm — Vedder Price P.C.; Thomas CI2O I/48 (2006.01) J. Kowalski; Heidi Lunasin CI2N 9/10 (2006.01) (52) U.S. Cl. ............................. 435/15; 435/49; 435/193 (57) ABSTRACT (58) Field of Classification Search .................. 435/193, A method for the in situ production of an emulsifier in a 435/15, 49 foodstuff, wherein a lipid acyltransferase is added to the See application file for complete search history. foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid (56) References Cited content of the foodstuff. 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