(12) United States Patent (10) Patent No.: US 7,955,813 B2 De Kreijet Al
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US007955813B2 (12) United States Patent (10) Patent No.: US 7,955,813 B2 De Kreijet al. (45) Date of Patent: *Jun. 7, 2011 (54) METHOD OF USING LIPID 3,939,350 A 2f1976 Kronicket al. ACYLTRANSFERASE 3,973,042 A 8, 1976 Kosikowski 3.996,345 A 12/1976 Ullman et al. 4,034,124 A 7, 1977 Van Dam (75) Inventors: Arno De Kreij, Papendrecht (NL); 4,065,580 A 12/1977 Feldman Susan Mampusti Madrid, Vedbaek 4,160,848 A T. 1979 Vidal 4,202,941 A 5/1980 Terada (DK); Jorn Dalgaard Mikkelsen, 4,275,149 A 6/1981 Litman et al. 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O3O112O2 Fernandez-Garcia et al. J. Dairy Sci. 77: 2139-2149 (1994).* Jan. 17, 2003 (GB) ...... ... O3O1121.0 Jan. 17, 2003 (GB) ...... ... O3O1122.8 (Continued) Dec. 24, 2003 (GB) ................................... O33OO16.7 Primary Examiner — Tekchand Saidha (51) Int. Cl. (74) Attorney, Agent, or Firm — Vedder Price P.C.; Thomas CI2O I/48 (2006.01) J. Kowalski; Heidi Lunasin CI2N 9/10 (2006.01) (52) U.S. Cl. ............................. 435/15; 435/49; 435/193 (57) ABSTRACT (58) Field of Classification Search .................. 435/193, A method for the in situ production of an emulsifier in a 435/15, 49 foodstuff, wherein a lipid acyltransferase is added to the See application file for complete search history. foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid (56) References Cited content of the foodstuff. 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