Chromatography & Mass Spectrometry

Chromatography & Mass Spectrometry Product Reference Guide

more capabilities. new possibilities. Thermo Scientific + Dionex Leading technologies • Applications expertise • Global support more capabilities. new possibilities.

Dionex products are now a part of the Thermo Scientific brand, bringing leading capabilities in ion chromatography (IC), liquid chromatography (LC), and sample preparation to the industry’s leading portfolio, creating new possibilities for scientific analyses. Our excitement about this winning combination is about more than adding the Dionex range of IC, LC, and sample preparation instruments to the Thermo Scientific portfolio. For over 30 years, Dionex has built a reputation for continuous innovation, for leading the industry with pioneering advances in separations chemistry, consumables and software, and for providing solutions to challenging analytical problems in diverse areas, including critical applications in environmental protection and those in life sciences. The following pages illustrate the range, scope and excellence of our combined offerings, where you’ll find the best of separation science meeting the leading mass spectrometry solutions to help move your science forward. table of contents + UHPLC for all LC Systems 2-3 + Orbitrap LC-MS 14-15 + UHPLC Accessories and Supplies 4-5 + Ion Chromatography (IC) 16-17 + HPLC and UHPLC 6-7 + IC and RFIC Accessories 18-19 + GC, GC-MS and ICP-MS 8-9 + Chromatography, MS and LIMS Software 20-21 + Columns and Accessories 10-11 + Sample Prep 22-23 + LC-MS 12-13 + Service and Support 24-25

Leading Optimized Informative Instruments accessories software new discoveries.

Liquid chromatography Gas chromatography Ion chromatography Mass Spectrometry Leading LC, UHPLC and Our portfolio of GC, GC-MS Standard, reagent-free IC and Systems for precise nano-LC systems and GC-MS/MS solutions capillary IC systems from the identification and quantification world-wide leader

Chromatography, MS Sample Prep ICP-MS and LIMS Software Better extractions in Outstanding productivity Faster data interpretation less time and greater confidence and information flow

Knowledgeable Timely Unsurpassed method service commitment development

1 more capabilities. new possibilities.

UHPLC delivers important benefits – faster runs, better resolution, and lower operating costs. With Dionex now part of the Thermo Scientific brand, UHPLC performance can be offered at standard HPLC prices to a greater number of laboratories across a wider range of applications.

The Dionex UltiMate 3000 HPLC system offers UHPLC compatibility across all modules, ensuring maximum performance. With flow rates from 20nL/min to 10mL/min and offering a wide range of pumping, sampling, and detection modules, the UltiMate 3000 series provides solutions for all your chromatography needs. Features and Benefits: • UHPLC design for all RSLC, nano UltiMate 3000 UltiMate 3000 and standard analytical systems Rapid Separation LC Systems RSLCnano Systems • 620 bar maximum pressure for basic and standard analytical The Dionex UltiMate 3000 Rapid The Dionex UltiMate 3000 RSLCnano systems – setting a new Separation LC (RSLC) systems systems have been designed to benchmark in HPLC integrate unique hardware features optimize low flow separations and • x2 Dual systems form a unique for ultrafast separations and excel- facilitate easy coupling to mass platform for routine analysis, lent resolution using columns with spectrometry to provide the best increased productivity solutions, small particles. In addition, they are resolution, sensitivity, and selectivity for and advanced chromatographic characterized by a maximum level of nano LC and proteomics applications. techniques reliability and flexibility. • Ideal for analysis with limited • Systems are controlled • Binary or quaternary systems for sample amounts ® using Dionex Chromeleon both UHPLC and conventional • High sensitivity to allow detection Chromatography Data System HPLC applications of femtomoles or even lower software – providing Intelligent • Extensive flow-pressure-footprint • Maximum robustness and Functionality and Operational for ultrafast, ultrahigh-resolution repeatability for consistent results ™ Simplicity separations • Increased resolution and selectivity ™ • Systems are offered with Viper • Column pressures up to 1000 bar for more reliable identification ™ and nanoViper fitting systems – • x2 Dual RSLC systems for ultimate • Widest nano/cap/micro flow range fingertight, zero-dead-volume productivity solutions from 20nL/min up to 50µL/min connections even at UHPLC pressures

2 more versatile UHPLC for all LC Systems

UHPLC compatibility has been added to all standard and basic automated Dionex HPLC systems, and we’ve extended the UHPLC performance of the UltiMate® 3000 RSLC systems, giving you more versatility for your analytical needs.

UltiMate 3000 UltiMate 3000 Standard LC Systems Basic LC Systems The Dionex UltiMate 3000 Standard The Dionex UltiMate 3000 Basic LC systems provide the right solution systems are cost-effective solutions for demanding, analytical, and for conventional HPLC and UHPLC. semipreparative LC applications. Fully-scaleable, modular basic • Optimal performance and reliability systems are designed to provide for conventional LC applications consistent, robust operation and • 620 bar maximum pressure are even compatible with UHPLC and 100Hz detectors for applications. UHPLC compatibility • Cost effective system for routine • Widest range of system applications configurations for maximum • 620 bar maximum pressure application flexibility and 100Hz data rate for UHPLC • Flow rates up to 10mL/min compatibility covering all application needs • Optional Autosampler Column • Biocompatible version available Compartment with integrated (up to 350 bar) sampling and temperature control

3 more capabilities. new possibilities.

Dionex LC modules integrate innovation and intelligent features into a broad selection of autosampler, injector, pump, flow-control, thermostatted column compartment, and detector modules. We offer a wide variety of LC and Bio columns for any analytical application, as well as accessories for use with our HPLC systems.

UltiMate 3000 Samplers and Pumps Column Compartments Detectors The Dionex UltiMate 3000 pump The Dionex UltiMate 3000 auto- Optical Detectors: The Dionex family offers the most complete samplers ensure reliable, precise, UltiMate 3000 optical detectors are choice in the industry. From nano and accurate injections of nL to mL available for UV-vis absorbance, LC to rapid separation applications, sample volumes, with extremely fluorescence, and refractive-index from conventional applications to low carry-overs. Fraction collection detection of a wide variety of analytes. UHPLC, the UltiMate 3000 pumps options range from simple collection Charged Aerosol Detectors: always provide industry-leading flow, to advanced collection-reinjection The Dionex ESA Corona® charged pressure and precision. workflows such as automated aerosol detectors provide universal off-line 2D-LC. Isocratic: Analytical detection of any non-volatile analyte Binary: RSLCnano, Analytical, RSLC Autosamplers: Split-Loop, and are compatible with gradients. Pulled-Loop with integrated Quaternary: Micro, Analytical, RSLC Electrochemical Detectors: column compartment Dual-Gradient: Micro, Analytical, The Dionex ESA Coulochem® III is an RSLC Fraction Collectors: Fraction electrochemical detector for easily Collector, Autosampler/Fraction oxidized or reduced analytes. It Collector, MALDI Spotter offers outstanding performance for Column Compartments: amperometric, coulometric, and Integrated Switching Valves pulsed amperometric detection.

4 Accessories and Supplies more comprehensive UHPLC

MSQ Plus Mass Spectrometer Viper Fitting System LC Columns The Thermo Scientific MSQ™ Plus The Viper and nanoViper fitting Nano Columns: A wide range Mass Spectrometer can be used in systems eliminate problems of dedicated Acclaim® PepMap™ a wide range of applications and experienced with conventional fitting nano columns with zero dead- methodologies in both HPLC and IC. systems. They provide a perfect fit volume connections Only 12 inches wide, the MSQ Plus each time and ensure superior Rapid Separation LC Columns: is a compact mass spectrometer chromatographic performance. Acclaim RSLC columns for ultimate providing universal detection and • Zero-dead-volume UHPLC speed and resolution in UHPLC characterization of analytes in the fingertight fittings for nano/cap, 17–2000 m/z range. Compatible Mixed-Mode Columns: micro, and analytical LC with existing LC and IC methods, Includes unique chemistries with • Compatible with virtually every type the MSQ Plus is sensitive, rugged, tunable selectivities for charged of valve and column hardware powerful, easy to use, and and non-charged analytes • Flexible stainless steel or fused integrates seamlessly with LC silica capillaries Bio Columns: Chemistries that and IC systems. include a unique monolith technology for separations of biomolecules

Specialty Columns: Customized selectivities for dedicated application requirements

Standard Columns: Acclaim columns with innovative bonding for a wide range of different selectivities

5 more capabilities. new possibilities.

The leader in LC-MS innovation provides additional liquid chromatography solutions for a range of dedicated analytical needs. Whatever your requirements, Thermo Scientific LC, UHPLC, nano-LC, and LC-MS systems deliver the performance your laboratory demands.

SpectraSYSTEM LC System Accela High Speed LC Accela Open Autosampler The Thermo Scientific SpectraSYSTEM Thermo Scientific Accela High Speed The Thermo Scientific Accela Open LC is an economical system that can LC is a robust modular system with Autosampler provides a versatile, be configured to match a range of a large suite of integrated features modular design for maximum application and performance demands. to increase application flexibility and application flexibility. Its automatic The Thermo Scientific SpectraSYSTEM efficiency. Designed for maximum per- open architecture enables easy provides a full range of degassers, formance, reliability and productivity, access to samples, syringe, valves pumps, autosamplers and detectors Accela provides seamless operation and injection ports. Configurable for in a compact, modular HPLC system. from conventional LC pressures up to any LC and LC-MS application, it Low delay volume and excellent 1250 bar for UHPLC separations of is designed to carry out sample gradient precision ensure highly complex matrices. injections automatically and reproducible chromatographic The new Accela 1250 pump features reliably with fast sample cycles, separations and compatibility with Force Feedback Control (FFC) which outstanding reproducibility and all column types. Available in standard eliminates the need for pulse damp- extremely low carryover. isocratic and gradient configurations. ening while improving flow accuracy and gradient precision under extreme operating conditions. It operates at a top pressure of 1250 bar with flow rates of up to 2mL/min, reducing run times and improving productivity.

6 more dedicated HPLC and UHPLC Systems

Transcend LX System EASY-nLC II EASY-nLC 1000 The Thermo Scientific Transcend™ Thermo Scientific EASY-nLC II The Thermo Scientific EASY-nLC LX series systems are designed to nanoflow liquid chromatograph 1000 is a split-free, nanoflow improve MS efficiency, productivity provides effortless split-free nano LC optimized for separating bio- and throughput. The LX-4 system chromatography excellence for molecules such as proteins and achieves the throughput of four LC-MS. peptides at ultra high pressures up separate LC-MS systems with only Easily installed and running within to 1,000 bar or 15,000 psi. The a single MS. The LX-2 system hours, the EASY-nLC II delivers un- instrument seamlessly integrates doubles the productivity of your rivaled ease-of-use and productivity with various mass spectrometers, mass spectrometer by achieving the for busy life scientists. The EASY- providing trouble-free operation. throughput of two separate LC-MS nLC II achieves precise, accurate EASY-nLC 1000 is a fully integrated systems with a single MS. and reproducible split-free chroma- LC-system with a binary nano flow • Accelerates results – achieve up tography that provides high resolu- gradient pump, a cooled auto- to a 4X improvement in mass tion results in even the most difficult sampler, switching valves and high spectrometry throughput of proteomics LC-MS analyses. precision flow sensors for accurate • Enhances productivity – analyze solvent control before more samples per hour high-pressure mixing. • Improves efficiency – mass spec idle less than 4 percent of the time • Increases flexibility – run up to four different assays at the same time

7 more capabilities. new possibilities.

Environmental, food safety, toxicology, and forensic gas chromatography applications are more demanding than ever. Our portfolio of GC, GC-MS, GC-MS/MS and ICP-MS is geared to meet the challenge.

TRACE GC ISQ Single FOCUS GC Gas Chromatographs Quadrupole GC-MS The Thermo Scientific FOCUS™ GC The Thermo Scientific TRACE GC The Thermo Scientific ISQ™ system is a compact, single-channel gas responds to the needs of modern offers rugged and reliable perfor- chromatograph designed for use in laboratories for higher productivity mance and nonstop productivity. routine quality control laboratories and increased sensitivity. The Thermo Scientific ISQ GC-MS and is available with either FID or The “Ultra” platform combines the features a new source design ideal TCD. Conceived and built around reliability of the TRACE GC with for continuous high-throughput oper- the “core” of the TRACE GC Ultra’s extended system usability, perfor- ation. The vacuum interlock enables hardware and unique features, the mance, and automation. It addresses source removal without venting the FOCUS GC is the reference for a wide range of large volume and system, for nonstop productivity. precision and accuracy of results, split/splitless techniques, overcoming as well as a standard for rugged- sensitivity boundaries of conventional ness for QA/QC and high throughput GCs without additional investment, environments. training or revalidation of methods. Can be configured with up to two injectors and three detectors, making it ideal for the analysis of crude oils, pesticides, hydrocarbons as well as food and flavor character- izations. Dedicated turnkey analyzers for chemical and petrochemical applications are available.

8 more productive GC and ICP-MS Systems

ITQ Series TSQ Quantum XLS Ultra XSERIES 2 GC-Ion Trap MS Triple Quadruple GC-MS/MS ICP-MS Thermo Scientific ITQ Series GC-Ion The Thermo Scientific TSQ Quantum The Thermo Scientific XSERIES 2 Trap MS offers outstanding full-scan XLS Ultra is the new “Gold Stan- ICP-MS offers outstanding produc- electron ionization sensitivity and dard” in GC-MS/MS. HyperQuad™ tivity in a quadrupole ICP-MS for upgradeability. technology delivers highly increased both routine and high performance From a small-footprint entry-level mass resolving quadrupoles for ultra analytical work. Laboratories can QA/QC instrument to a fully-featured, selective SRM, with best in class achieve analytical objectives faster, research-grade system with sensitivity, and allows unsurpassed with greater confidence and less advanced MSn functionality, the analytical performance for the most hands-on time from the operator. 2 Thermo Scientific ITQ Series offers difficult matrix challenges. TSQ Advanced H /He collision cell tech- a broad range of standard features Quantum XLS Ultra is engineered to nology provides uncompromised along with an impressive list of meet the requirements of the most multi-element analysis for all elements options. As needs change, the ITQ demanding analytical tasks across including Ca, Fe, As and Se, with Series offers upgrade options to food safety, environmental, clinical, outstanding signal/background. expand instrument capabilities and forensic, toxicology, pharmaceutical add flexibility and versatility. and metabolomics and applications.

9 more capabilities. new possibilities.

Discover the most comprehensive array of chromatography solutions available. From sample preparation to separation and analysis, Thermo Scientific tools help you achieve repeatable, predictable results – separation after separation.

Thermo Scientific Thermo Scientific Thermo Scientific GC Columns and Accessories LC Columns and Accessories Sample Preparation Solutions Redefine your expectations for Hypersil GOLD LC Columns: Our sample preparation solutions GC with our line of GC Columns • Excellent peak shape remove uncertainty by applying TraceGOLD GC Columns provide a • Increased sensitivity science to SPE. leap forward in column performance • Improved resolution HyperSep Retain: offering fast by delivering low bleed and superior • 1.9 to 12μm particles and easy sample preparation. inertness. Hypercarb LC Columns: 100% WebSeal: products for high TracePLOT GC Columns offer the porous graphitic carbon provides throughput screening. latest innovation in PLOT column extended separation, exceptional retention of very polar analytes. QuEChERS: solutions for efficient technology, providing reproducible sample preparation and clean-up. analyses of permanent gases, • pH stable from 0 to 14 hydrocarbons and solvents. • Ideal for high temperature eVol: the world’s first analytical syringe. GC Accessories: Columns for Fast LC: 1.7µm All the tools necessary for today’s Syncronis and 1.9µm Hypersil GOLD gas chromatographer. columns provide high speed, high efficiency, excellent peak shape and consistent separations. Columns for Biomolecules: BioBasic reversed phase, ion exchange and size exclusion columns are ideal for the analysis of a wide range of biomolecules.

10 GC, HPLC and SPE more versatile Columns and Accessories

For more information, visit the Chromatography Resource Center, an extensive and fully searchable library featuring over 6000 applications and references encompassing liquid chromatography, gas chromatography and solid-phase extraction. thermoscientific.com/chromatography

Thermo Scientific Thermo Scientific Thermo Scientific Chromatography Tools GC Derivatization Reagents and Vials and Closures and Supplies Standards, Reacti-Therm System A comprehensive range of solutions Enhance and optimize the Derivatization reagents for mass for any instrument and any application. performance of your spectrometry, high-pressure Certified and Mass Spec chromatography instruments. liquid chromatography and gas Certified Vials: • Autosampler Syringes chromatography. • Unmatched consistency • Chromatography Standards – Reagents designed to provide • Unique pre-cleaned vials Complete sets of chemical selectivity and improve sensitivity. and packaging standards available for each • Derivation and Visualization • 13 tests ensure highest integrity EPA method Reagents for HPLC • Micro sampling options • Connectors • GC Derivatization Reagents • Headspace vials • Ferrules • HPLC and Specrophotometric • High recovery vials • Gas Filters Grade Solvents • Tools and accessories • Gas Tight Syringes • HPLC Ion Pair Reagents • Hydrolysis Tubes • Other HPLC Reagents • Port Injection Liners • Siliconizing Fluids • Septa

11 more capabilities. new possibilities.

Robust Thermo Scientific triple quadrupole LC-MS systems enable the quantitation and confirmation of compounds in complex matrices.

TSQ Quantum Access MAX TSQ Quantum Ultra TSQ Vantage Triple Stage Quadrupole Triple Stage Quadrupole Triple Stage Quadrupole Mass Spectrometer Mass Spectrometer Mass Spectrometer Thermo Scientific TSQ Quantum Thermo Scientific TSQ Quantum Thermo Scientific TSQ Vantage Access MAX triple stage quadrupole Ultra mass spectrometer provides triple stage quadrupole mass mass spectrometer is the value- maximum productivity for bio- spectrometer delivers the highest conscious choice for a wide variety analytical analysis. sensitivity with the lowest noise for of LC-MS applications, including Faster method development at the quantitative analysis of small pharmaceutical, environmental, the highest possible specificity is molecules, peptides, biosimilars, food safety, clinical research, and routinely achievable with the TSQ and biologics. forensic toxicology. Quantum Ultra mass spectrometer. • Precise, consistent and • Best in class mass range of m/z reproducible results at amazingly • Advanced technology 10 - 3000 low levels of quantitation facilitates development of robust • Up to 3000 timed SRMs with fast • Robust new ion source, second quantitative methods positive/negative mode switching generation (G2) ion optics and • High selectivity significantly of ≤25ms hyperbolic quadrupoles reduces matrix interferences • Precise, consistent and • Provides a 10X S/N improvement reproducible results at amazingly over competing systems low levels of quantitation • 1,000 SRM/sec, 3,000 timed SRMs per LC run • >4 orders linear dynamic range

12 more robust LC-MS Systems

Versatile ion trap LC-MS systems offer unique capabilities such as MSn and data dependent analysis along with excellent full scan sensitivity to provide routine detection and rapid identification of low level analytes.

LCQ Fleet LTQ XL Linear Velos Pro Ion Trap Mass Spectrometer Ion Trap Mass Spectrometer Ion Trap Mass Spectrometer The cost-effective Thermo Scientific Thermo Scientific LTQ XL ion trap With the improved robustness of LCQ Fleet Ion Trap Mass Spec- LC-MS mass spectrometer delivers Generation 2 ion optics, the Thermo trometer delivers rich information for high MSn high performance to gener- Scientific Velos Pro ion trap mass routine analysis of complex samples ate extensive structural information spectrometer delivers reliability on while providing excellent full-scan for routine proteomics and metabo- the fastest, most sensitive, highest sensitivity, ruggedness and reliability. lism applications. Its high capacity capability ion trap available today. • Proven performance for compound 2D linear ion trap configuration with • New detection electronics enable identification in complex matrices powerful software is a complete linear quantitation of up to 6 orders • Easy to use single-point LC-MSn solution for structural elucidation of magnitude for enhanced repro- for non-MS operators questions. ducibility and confidence in results LTQ XL offers multiple dissociation • Fast scanning up to 66,000 Da/ techniques, PQD, ETD and CID. sec enables ultra high-throughput • PQD is a proprietary technique that analyses, compatible with the eliminates the low mass cut-off fastest UHPLC systems concern inherent with all ion traps • New Trap-HCD fragmentation offers • Extensive coverage for predicted complementary, triple quadrupole- and unpredicted metabolites, like fragmentation that facilitates and the ability to perform peptide structural elucidation, sequence quantification using iTRAQ™ labels assignment and quantitation of isobarically labeled peptides

13 more capabilities. new possibilities.

Orbitrap technology is the recognized gold standard for high-resolution, accurate mass (HR/AM) anaylses. It is available in a variety of mass spectrometry platforms, from routine screening tools to ultra-high performance research instruments.

The Technology of Choice for Accurate Mass and High- Resolution Measurement Since its commercial introduction in 2005, Orbitrap technology has become the recognized standard for routinely achievable ultra-high resolution (>100,000) mass spectrometry with reliable accurate mass. Combined Exactive High Performance Q Exactive High Performance with superior dynamic range and Benchtop LC-MS Benchtop LC-MS unsurpassed sensitivity, Orbitrap- Mass Spectrometer Mass Spectrometer based platforms are the only LC-MS Thermo Scientific Exactive Mass Thermo Scientific Q Exactive™ and MS/MS systems capable of Spectrometer is an easy-to-use benchtop Orbitrap system combines providing all four benefits at the same benchtop system combining high-performance quadrupole pre- time, without compromise. premium performance with a simple, cursor selection with high-resolution, The addition of an ion trap mass intuitive interface, resulting in a accurate-mass (HR/AM) Orbitrap analyzer to an Orbitrap detector LC-MS system that is smaller, faster detection enabling identification, enables multiple levels of fragmenta- and affordable for virtually any lab. quantitation and confirmation of com- tion (MSn) as well as multiple • High Resolving Power (up to pounds in a single run, an innovative fragmentation modes (CID, HCD, 100,000) provides precise capability known as “quanfirmation.” and ETD) for the elucidation of mass accuracy for complex The Q Exactive can identify, quantify analyte structures. Coupling with sample analysis and confirm more trace-level proteins, continuous ionization sources such • Mass accuracy of better than peptides, metabolites and contami- as atmospheric pressure chemical 2ppm in full scan using fully nants in complex mixtures. ionization source (APCI), electrospray automated AGC and mass • Resolving power of up to 140,000 (ESI), or nanoelectrospray (NSI), calibration procedures FWHM provides high confidence or with matrix-assisted laser • Scan Speed operating at a 10Hz • High scan speed and desorption/ionization (MALDI) scanning frequency Exactive is fully multiplexing capabilities fit provides increased analytical compatible with UHPLC and en- with UHPLC timescale flexibility and power. sures exact mass measurement for • Fast positive-negative scan-to- fast chromatography applications scan polarity switching enables detection of the widest possible range of compound types

14 more resolution Orbitrap LC-MS Systems

LTQ Orbitrap XL Orbitrap Velos Pro Orbitrap Elite Thermo Scientific LTQ Orbitrap XL Thermo Scientific Orbitrap Velos Pro Thermo Scientific Orbitrap Elite hybrid mass spectrometer supports a hybrid mass spectrometer blends combines a novel high-field Orbitrap wide range of applications from rou- the unsurpassed speed and sensitiv- with the Velos Pro dual-pressure tine compound identification to the ity of the Velos Pro dual-pressure linear ion trap to deliver unsurpassed most challenging analysis of low level ion trap with the ultra-high resolution resolving power, increased sensitiv- components in complex mixtures. and outstanding mass accuracy of ity, high scan speeds and a larger Excellent mass accuracy, mass reso- Orbitrap™ technology. dynamic range. n lution and high sensitivity MS make it • High mass resolution (>100,000) • Resolving power of >240,000 ideally suited for general proteomics and mass accuracy (1ppm) FWHM provides unprecedented and metabolism applications. allows accurate measurement and confidence • Based on the fast and highly identification of intact proteins • Highest resolution for meaningful sensitive Thermo Scientific LTQ XL • Outstanding performance in top-down protein characterization linear ion trap and the proprietary multiplexed experiments using experiments Orbitrap technology isobaric labels • Highest sensitivity for the detection • New HCD collision cell for greater • Stability, mass accuracy and of very low abundance proteins, flexibility in fragmentation experi- wide dynamic range required for peptides and metabolites ments for advanced proteomics successful label-free analyses and small molecule research • Parallel MS and MSn analysis • Can be upgraded to include ETD • Multiple fragmentation techniques: and MALDI capabilities CID, HCD, and optionally ETD

15 more capabilities. new possibilities.

Since 1975, the Dionex brand has been synonymous with the forefront in ion chromatography. Continuous Dionex innovations in instrumentation, chemistry, applications and software ensure that you get the best IC solutions available –

Reagent Free Ion Chromatography (RFIC™) Systems Deliver Superior Results Dionex IC technology has evolved over many product generations, each providing enhanced performance, greater reliability, and easier operation. Reagent-Free™ IC eliminates daily tasks of eluent and regenerant preparation, saving time, preventing ICS-5000 Modular ICS-2100 errors, and increasing convenience. RFIC-EG System RFIC-EG System RFIC-EG systems use electrolytic technologies to generate eluent on The world’s most advanced IC system This compact integrated Dionex IC demand from deionized water, and provides unmatched capability, flex- makes its own eluent from deion- to suppress the eluent back to pure ibility, and convenience. Available in ized water. Just add water, and get water. They deliver unmatched single or dual configurations in excellent results for a wide range of sensitivity and the separating power analytical-scale and/or capillary conductivity-based methods using of gradient IC, all with “just add formats, and with a broad selection isocratic or gradient elution. water” convenience. of detectors, the Dionex ICS-5000 Key Features: provides optimum performance for • Compact design conserves bench Capillary IC takes convenience to any IC application. a new level. Using only 5L of eluent space while providing easy access per year, and needing no service or Key Features: to fluidics calibration for months at a time, a • Modular design adapts to diverse • Touchscreen LCD display allows capillary ICS-5000 is always ready to and changing analytical needs convenient front-panel control run samples. It’s the world’s first IC • RFIC-EG, plus quaternary • Integrated eluent generator that enables true walk-up analysis gradient proportioning and produces precise gradients on on demand. vacuum degasser, provides demand – just add water! ultimate eluent flexibility • Column heater reduces noise RFIC-ER systems use electrolytic • Extremely precise temperature and drift technologies to suppress the eluent control dramatically reduces prior to detection, and to continu- noise and drift ously regenerate eluent from the column effluent. With RFIC-ER, you can run routine applications for a month on a single batch of eluent.

16 more confidence Ion Chromatography Systems

solutions that deliver reliable analytical results while saving you time and money. A range of modular and integrated systems gives you the flexibility to choose the level of features and performance that’s right for your application and your budget.

ICS-1600, ICS-1100 ICS-900 RFIC-ER Systems Basic IC System Compact, integrated Dionex ICs • RFIC-ER option allows continuous This compact, entry-level unit delivers provide good performance for operation for up to a month using good performance for basic Dionex common conductivity-based a single bottle of eluent IC applications using manually IC methods. • Column heater reduces noise and prepared eluents. drift (ICS-1600) Key Features: Key Features: • Electrolytic suppression effortlessly • Compact design conserves bench • Compact design conserves bench improves signal/noise ratio space while providing easy access space while providing easy access • Support for standard-bore and to fluidics to fluidics microbore formats offers flexibility • Touchscreen LCD display allows • Displacement Chemical • Optional vacuum degasser saves convenient front-panel control Regeneration suppression labor and prevents cavitation (ICS-1600) provides low noise and (ICS-1600) stable baselines • Support for standard-bore and microbore formats offers flexibility • Optional external column heater reduces noise and drift

17 more capabilities. new possibilities.

Staying the leader in IC for over 35 years requires more than having the best instruments. Dionex products encompass not only instruments, but also industry-leading automation, software, and chemistries – all of which work together to provide you with comprehensive

Eluent Generation Self Regenerating Autosamplers Cartridges (EGC III) Suppressor (SRS) A selection of Dionex IC auto- The EGC III cartridge generates Suppression works two ways to samplers, all with metal-free flow high-purity hydroxide, carbonate, or achieve the absolute best sensitivity paths, supports diverse application methanesulfonic acid (MSA) eluents and corresponding lowest detec- and budgetary requirements. electrolytically. Stop spending time tion limits for inorganic analyses; it AS-AP: This high-performance manually preparing eluents. With increases analyte signal while simul- autosampler provides increased EGC, you just add water. taneously decreasing background sample capacity, fast injection times, Key Features: signal and noise. The SRS 300 maximum precision, and broad • Simplified operation; no need to supports virtually all analytical scale application flexibility. Inject from vials prepare eluents or regenerants ion chromatography applications and/or wellplate positions in any • Improves analytical reproducibility, for both anions and cations. desired order, automate sample day-to-day, week-to-week, Key Features: preparation, and deliver samples month-to-month • Low background noise levels to one or two instruments. • Ensures system-to-system • Fast startup equilibration times AS-DV: This economical autosampler reproducibility and lab-to-lab • Trace anion and cation automatically filters samples as consistency determinations it delivers them. It supports 5mL and/ • Achieves sensitive results with • Compatibility with mass or 0.5mL vials, and can load sample pure, uncontaminated eluent spectrometry detection onto an injection loop or concentrator • Eliminates errors and variability • Compatibility with all Dionex ICS column. associated with manual eluent and DX chromatography modules and regenerant preparation • A three-fold increase in back- AS-HV: This versatile autosampler pressure tolerance compared supports a wide range of sample to previous generations containers, and is ideal for applications involving trace-level contaminants in high-purity water.

18 more applications IC and RFIC Accessories

solutions to your analytical challenges. When you need an analytical solution for inorganic ions, organic acids, amines, sugars, proteins, peptides, or other challenging species, find out what we can do for you.

Continuously Regenerated Carbonate Removal IonPac® Trap Column (CR-TC) Device (CRD) Chromatography Columns Designed for eluent generators in The Carbonate Removal Device At the heart of Dionex ion chroma- RFIC systems, CR-TC columns (CRD) removes carbon dioxide from tography is a unique set of column remove all anionic or cationic con- the suppressed eluent stream by chemistries that provide high taminants in the eluent continuously diffusion through the walls of a gas selectivities and efficiencies with and provide very low baseline drift permeable membrane. With excellent peak shapes. during gradient operations. carbonate eluent systems, it Hydroxide-Selective Anion- Key Features: reduces background signals to Exchange Columns: For isocratic • Generates contaminant-free nearly the same levels as those of and gradient separations with a wide deionized source water and eluent hydroxide eluents. range of capacities and selectivities • Time savings—no need to perform Optimized for the removal of carbon- Carbonate Eluent Anion-Exchange regeneration off-line ate from hydroxide eluent systems: Columns: Provides well-characterized • Very low baseline drift for improved • Improves quantitation by isocratic separations for regulated integration and increased sensitivity minimizing carbonate drinking water and wastewater • Increased productivity; quality data • Lowers backgrounds, providing methods soon after startup higher sensitivity • Removal of carbonic acid Cation-Exchange Columns: • Eliminates carbonic acid, Available in a wide range of contaminants from source water increasing the linear range • Compatibility with Capillary capacities and hydrophobicities for RFIC-EG systems isocratic and gradient applications Ion-Exclusion Columns: Allows separation of weak acids-with strong acids eluting in the void

19 more capabilities. new possibilities.

Our enabling software solutions include enterprise-level offerings for global deployment, as well as software designed to maximize the functionality and efficiencies of our world- class scientific instruments.

Thermo Scientific Chromatography Data Systems Mass Spectrometry Software Application-Specific Software Chromeleon® Software Thermo Scientific Xcalibur Software Proteomics No other data system comes close This flexible Windows®-based data Proteome Discoverer: for analysis to providing the capabilities and the system provides instrument control of qualitative and quantitative data. usability of Dionex Chromeleon – it’s and data analysis for all Thermo ProteinCenter: for web-based ™ Simply Intelligent . The software is Scientific mass spectrometers. With comparison and interpretation of designed to take users from samples extensive functionality, the ability to data sets in minutes. to results in the shortest possible integrate third-party control, and avail- ProSightPC: for top-down, middle- time. Sequence set-up and process- ability of several application-specific down and bottom-up experiments. ing, and result calculations can all be modules, Xcalibur can address a wide Pinpoint: for easy transition from performed quickly, easily, and with- range of applications. early-stage biomarker discovery out training. It controls IC, LC and DCMSLink to larger-scale, quantitative and GC instruments from a wide range of A free, control-only Dionex software quantitative proteomics. manufacturers. package, providing fully integrated SIEVE: for label-free, semi- Atlas and ChromQuest CDS single-point control of any Dionex LC quantitative differential Other Thermo Scientific CDS solutions system through Xcalibur and mass expression analysis. include Atlas, an enterprise level, spectrometry control software from mission critical client/server applica- other leading manufacturers. Metabolomics/Metabolism tion that is flexible and scalable from MetQuest: for automated quan/qual single instrument workstations to metabolic screening using high reso- multichannel, multi user client/server lution, accurate mass Orbitrap data. implementations. It also supports MetWorks: for automated instruments from a range of manu- metabolite identification and the facturers. ChromQuest is a powerful search for biotransformations. CDS that can meet a wide range of LC and GC laboratory needs. It can be deployed in a variety of flexible configurations from single user/single instrument workstation to multi-user/ networked instruments.

20 Chromatography, MS and LIMS more connected Software

From single workstations to enterprise solutions, our CDS and MS solutions provide the flexibility to fully integrate with our laboratory information management systems (LIMS) and other enterprise-level data management systems.

Thermo Scientific Lab Information Management Systems Other Software Drug Discovery and Development SampleManager LIMS: an NextDocs Document Management: QuickQuan: for high-throughput enterprise LIMS for company quality a cost-effective, intuitive, flexible and analysis in early drug discovery. control operations. scalable solution based on Microsoft LCQUAN: for LC-MS data Darwin LIMS: purpose-built LIMS Sharepoint. management in a 21 CFR Part 11 for pharmaceutical manufacturing; Accelerys Electronic Laboratory compliant environment. reduces validation time required for Notebook and Decision Support: QuickCalc: for fast evaluation of new regulatory compliance. replaces paper notebooks scientists compounds in drug development. Watson LIMS: for DMPK/Bioanalyti- and researchers use to document, design, execute and analyze TSQ Module: integrates Watson cal studies in drug development. experiments. LIMS with Thermo Scientific triple Nautilus LIMS: for pharmaceutical, quads to simplify workflows in biobanking, forensics and food labo- Thermo Scientific Kinetica: a data regulated laboratories. ratories with complex plate-handling analysis application with a library of requirements, allowing users to map prebuilt, ready-to-use PK, TK, and Forensics/Toxicology and track processes with ease. PK/PD models for pharmacokinetic, ToxID: for automated LC-MS/MS pharmacodynamic studies. screening in forensic toxicology. Galileo LIMS: for screening ADME properties in drug development. Dionex D-Library: an application ToxLab and ToxLab Forms: for Provides data graphing and visualiza- library using web 2.0 technologies automated GC-MS acquisition, tion for large volumes of compounds. to give customers maximum analysis and reporting for toxicology. LIMS-on-Demand: affordable accessibility to LC and IC applica- General Screening Applications web access to a laboratory from tions based on search criteria such ExactFinder: provides a single anywhere, at any time, from any as analyte, matrix, market, run time, streamlined workflow for targeted web browser. column or instrument type. and general unknown screening Integration Manager: flexible, experiments using high resolution, data transformation tool that accurate mass Orbitrap data. automates data acquisition, and TraceFinder: for managing distributes point-to-point data across trace analysis workflows in the enterprise. testing laboratories. Mass Frontier: manage, evaluate, and interpret GC and LC mass spectral data. 21 more capabilities. new possibilities.

Solvent extractions that normally require labor-intensive steps are automated and performed in minutes, with reduced solvent consumption and reduced sample handling using Dionex Accelerated Solvent Extraction (ASE®) and AutoTrace® systems.

Accelerated AutoTrace 280 Automated Solvent Extraction (ASE) Automated SPE On-line SPE-LC Dionex ASE® is a patented technique The Dionex AutoTrace® 280 A solution kit for the Dionex for the extraction of analytes from instrument performs automated UltiMate 3000 x2 Dual HPLC solid and semisolid sample matrices solid-phase extractions (SPE) of systems provides fully automated using common solvents at elevated large-volume liquid samples for on-line solid-phase extraction (SPE) temperatures and pressures. organic analysis. Liquid – liquid for high-sensitivity analysis from Compared to techniques such as extractions that normally take even complex matrices. Samples Soxhlet and sonication, ASE gener- hours can be automated using are injected directly onto an SPE ates results in a fraction of the time. an AutoTrace SPE system. column, the matrix is removed, and Benefits: the enriched analyte transferred to Benefits: an analytical column. • Extractions for sample sizes • Automated extractions for liquid 1–100g in minutes sample sizes of 20mL–20L Benefits: • Dramatic reduction in solvent usage • Dramatic reduction in solvent usage • Direct injection of • Wide range of applications and reduced sample handling untreated samples • Corrosion-resistance components • Wide range of applications • Fast analysis and • Approved for use by the U.S. EPA • Approved for use by many high reproducibility and CLP Program government agencies • Full automation for • Walk away automation for • Compatible with disk or unattended operation unattended operation cartridge configurations • Reduction of health risks • Hood-free operation • Extraction of 6 channels • Highest productivity simultaneously

22 more efficientSample Prep Systems

ASE systems are dramatically faster than Soxhlet, sonication, and other extraction methods, and require significantly less solvent and labor. ASE methods are accepted and established in the environmental, pharmaceutical, food, polymer and consumer product industries. ASE methods are accepted and used by government agencies worldwide.

OnGuard InGuard SolEx

Rocket IC Sample Sample Preparation Evaporator Preparation Solutions Accessories The Dionex InGuard™ inline and A revolutionary solvent evaporator for Products include solutions OnGuard® II offline cartridges use in laboratories seeking to spend for IC sample preparation with remove matrix interferences minimal time and effort to process AutoPrep and Reagent Free Ion such as phenolics, metals, cations, multiple samples for analysis. It can Chromatography – Electrolytic anions, or hydrophobic substances dry or concentrate up to six 450mL Sample Preparation (RFIC-ESP). encountered in many ion chromatog- flasks, or 18 ASE vials. This enables AutoPrep automatically prepares raphy applications. the user to focus on other tasks, calibration curves and performs OnGuard II Cartridges: Remove confident that the Rocket will sample analyses. Unique plumbing matrix interferences such as phe- achieve perfect, reproducible configurations and automated nolics, metals, cations, anions, or results every time. valve operations simplifies trace hydrophobic substances, for better Benefits: level analysis. performance in many IC applications. • Vacuum and centrifugal evapora- RFIC-ESP systems enable a range InGuard Cartridges: This line of tion for controlled evaporation and of automated sample preparation sample pretreatment cartridges minimized sample loss techniques using proprietary elec- removes matrix interferences such as • ASE and AutoTrace compatible trolytic devices to reduce cost and cations (including transition metals), vessels reducing solvent handling provide higher value analyses. • End point detection anions, or hydrophobic substances • Superior solvent recovery RFIC-ESP devices and techniques encountered in many IC applications. can remove cations from an anion SolEx™ Solid Phase Extraction sample before analysis using Cartridges: Offers a variety of chem- Dionex CR-TC devices, or neutrali- istries and sizes to fit your needs. zing a strongly acidic or basic solution.

23 more capabilities. new possibilities.

We understand the daily challenges you face – finding ways to increase productivity and reduce costs, while achieving better results in less time. The long-term performance of instruments and total cost-of-ownership will depend on the maintenance strategy selected to keep systems operating at peak efficiency. Capitalize on Thermo Scientific strengths in multiple service methods to optimize your performance and cost savings.

Maximize Resources Reduce Costs Increase Productivity We will work with you to build a A proactive maintenance strategy, To ensure consistent productivity, maintenance strategy leveraging the based on a Support Plan, maximizes consider the close, personal atten- best combination of services to meet your technology investment by tion and rapid response you receive your needs. Whether you are looking predicting and preventing problems with an instrument Support Plan. to service one or several instruments, before they occur. As a result, the life It will eliminate the guesswork and from one or many manufacturers, on of instruments is extended, overall unnecessary charges to your one bench or across an entire labo- laboratory productivity remains operating budget, as well as the cost ratory, we have a service solution to high, and total cost of ownership of replacement parts. Whether you meet your needs: is greatly reduced. have a hardware issue or a critical • Installation, Repairs and Calibration application question, you can rely • Support Plans on experienced, factory-trained • Preventative Maintenance engineers to troubleshoot repairs, • Certification/Compliance Services replace common-wear items, • Technical Support fine tune components, diagnose • Depot Repair problems, and implement • Training and Education Services corrective actions that will help • Parts, Accessories you avoid potential downtime and Consumables that will delay your efforts. • Custom Multi-Vendor Services

24 more options Service and Support

We manage your instruments so you can focus on the science. To improve your laboratory’s efficiency, you should be focused on your work, not managing instrument service. When you buy a Thermo Scientific product, you gain the peace of mind that comes from being backed by the largest team of service experts committed to your long-term success. We offer programs to service and maintain multiple instruments, regardless of manufacturer. Let our expertise complement yours. www.thermoscientific.com www.thermoscientific.com/dionex

Thermo Fisher Scientific, San Jose, CA USA is ISO Certified. Thermo Fisher Scientific (Bremen) GmbH Management System Registered to ISO 9001:2008 www.thermoscientific.com ©2011 Thermo Fisher Scientific Inc. All rights reserved. iTRAQ is a trademark of Applera Corporation. Windows® is a registered trademark of Microsoft Corporation in United States and other countries. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.

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