<<

Product Specifications

of application-specific templates for commonly determination for intact proteins and confident resolution of isobaric species. The unsurpassed scan rate and resolution of the system are especially useful when dealing with complex , in samples low-abundance and and , , , applications. similar The intuitive user interface of the tune editor and method editor makes instrument calibration Theand method method development easier. editor also contains library comprehensive a followed ETD by or EThCD for glycopeptide characterization or HCD followed CID by for small- structural analysis. The ultrahigh resolution of the analyzer increases certainty analytical of results, enabling molecular-weight performed analyses.

, with n acquisition , with n n experiments such as HCD n to maximize the amount of high-quality data acquired Next-generation sources and ion operation of ease system increase optics robustnessand Innovative instrument control software makes methods setup easier, more powerful, and operation more intuitive Tribrid architectureTribrid combines quadrupole, Multiple fragmentation techniques—CID, HCD, and optional ETD and EThCD—are available at any stage of MS linear , and Orbitrap mass analyzers subsequent mass either the analysis ion in trap or Orbitrap mass analyzer Parallelization of MS and MS •  •  The Orbitrap MS Fusion can Tribrid perform a wide variety of analyses, from in-depth discovery experiments characterization to of PTMscomplex qual/quan comprehensive to workflows. availabilityThe multipleof fragmentation techniques—CID, HCD, ETD, and EThCD—at any stage of MS •  •  •  fragment ion detection either in the ion trap or Orbitrap mass offers analyzer, a new level of versatility and performance for the most applications. Userschallenging can perform complex MS Unmatched analytical performance, Unmatched revolutionary MS architecture architecture ™ mass ™ Tribrid ™ Orbitrap Fusion

Thermo Scientific OrbitrapFusion Mass Tribrid ™ MASS revolutionary Thermo Scientific Orbitrap, and mass analysis in a spectrometer the best combines quadrupole, of The Thermo Scientific molecular composition more thoroughly. more composition molecular quantify them more accurately, and elucidate structure—to identify more compounds faster, high complexity, or difficult-to-analyze chemical challenging samples—samples abundance, challenging low of It enables lifescientists working with even the most that unprecedented delivers depth analysis. of 2

The optional Thermo Scientific™ EASY-ETD™ Dual-Pressure Linear Ion Trap Performance Characteristics uses Townsend discharge n • High-pressure cell for MS precursor ion Linear Ion Trap Mass Range to generate a highly stable reagent isolation from 0.2 amu to 600 amu Standard – m/z 50–2,000 ion flux. The source is very compact, fitting • Collision-induced dissociation (CID) and High Mass – m/z 200–4,000 inside the mass spectrometer. Intelligent, -transfer dissociation (ETD) automated precursor ion sorting routines, Orbitrap Mass Range reagent filtering using the quadrupole mass • Low-pressure cell for improved scan speed, Up to m/z 6000 without precursor ion resolving power, and mass accuracy filter, and charge-state-specific calibration of selection • Dual-dynode detector with high linear ETD reaction time maximize the quality of dynamic range for improved quantitation Resolution ETD spectra. and a large surface area for increased 15,000–500,000 (FWHM) at m/z 200 lifespan and robustness Hardware Specifications Scan Rate (under defined conditions) Vacuum System n Ion Source Orbitrap MS up to 20 Hz • Split-flow turbomolecular pump controlling Ion trap MSn up to 20 Hz Thermo Scientific™ EASY-Max NG™ vacuum in multiple regions Ion Source • Precise and accurate low mTorr pressure Mass Accuracy (under defined conditions) • Outstanding performance without control in the ion-routing multipole <3 ppm RMS using external calibration adjustments • Differentially pumped to ~3 × 10-4 Torr in <1 ppm RMS using internal calibration • Automatic gas and electrical connections the ion trap chamber MS/MS Ionization (ESI) on installation -10 • Differentially pumped to <2 × 10 Torr in Ion Trap Sensitivity • Enhanced exhaust port efficiently removes Orbitrap mass analyzer 2 μL of a 50 fg/μL solution of reserpine solvent vapor, improving uptime and (100 fg total) injected at a flow of 500 μL/min reducing chemical noise Options will produce a minimum signal-to-noise ratio ™ ™ Ion Optics Thermo Scientific Ion Max NG Ion Source of 100:1 for the transition of the isolated • Adjustable heated protonated molecular at m/z 609 to S-Lens (HESI) probe for ultimate sensitivity the largest two product ions, m/z 397 and • Progressively spaced stacked-ring ion • Flow rates from 1 μL/min up to m/z 448, when the mass spectrometer is guide (S-Lens) captures and efficiently <2,000 μL/min operated at unit resolution in the full-scan focuses ions in a tight beam MS/MS mode, m/z 165–615. The test • APCI probe compatible with liquid flow requires the HESI probe. • Large variable spacing between electrodes rates of 50 μL/min to 2 mL/min without provides better pumping efficiency and splitting Dynamic Range improved ruggedness ™ ™ >5,000 within a single scan • Automatic tuning program optimizes Thermo Scientific NanoSpray Flex NG transmission Ion Source MS Scan Power • Supports static packed-tip and dynamic MSn, for n = 1 through 10 Active Beam Guide nanoelectrospray experiments • Reduces noise by preventing neutrals and • Compatible with liquid flow rates of Synchronous Precursor Selection high-velocity clusters from entering the 50 nL/min to 2 μL/min Up to 20 precursors per MS2 scan, for MS3 quadrupole mass filter analysis only • Axial field along the length of the rods Thermo Scientific™ EASY-Spray™ Ion Source improves ion transfer robustness • Maximum nanoelectrospray performance Multiplexing Using Ion-Routing Multipole with no need for adjustments Up to 10 precursors per scan using Quadrupole Mass Filter quadrupole mass filter • MS/MS precursor ion selection with high Thermo Scientific™ EASY-ETD™ Ion Source ion transmission from 50 to 3000 m/z • Generates fluoranthene anions for electron Polarity Switching One full cycle in 1.1 sec (one full scan in • Precursor ion isolation window widths from transfer dissociation (ETD) positive mode and one full scan in negative 0.4 amu to 1200 amu • Townsend discharge ionization is extremely mode at resolution setting of 30,000) stable and robust Ion-Routing Multipole • Compact size, located entirely within the ETD Option • Higher- collisional dissociation footprint of the instrument ETD fragmentation efficiency of >15% (HCD) cell • Active reagent ion filtering using (infusion of 1 pmol/µL angiotensin at 3 µL/min) • Ion storage with or without fragmentation mass-resolving quadrupole • Efficient and stable ion transfer between Analog Inputs the ion-routing multipole and the Orbitrap Thermo Scientific™ EASY-IC™ Ion Source One (1) analog input (0–1 V), and linear ion trap mass analyzers • Generates internal calibrant ions for One (1) analog (0–10 V) real-time mass calibration on every Orbitrap Mass Analyzer -filled C-Trap • Provides <1 ppm RMS mass accuracy • Ultra-high-field Orbitrap mass analyzer with resolving power up to 500,000 FWHM and isotopic fidelity up to 240,000 FWHM at m/z 200 3

Figure 1. Orbitrap Fusion ion path

RF-Lens

Software Features Data System Exclusive Technologies • High-performance PC with Intel® • Dynamic Scan Management allows for • Data-dependent neutral-loss experiment microprocessor intelligent, real-time scheduling, triggers MS3 scans on only MS/MS • High-resolution LCD color monitor parallelization, and prioritization of the scan product ions with a predefined neutral loss events. It also enables selection, sorting, • Microsoft® Windows® 7 operating system • Isolation window offset allows for custom and routing of precursors to different centering of the isolation window when • Microsoft Office 2010 software fragmentation modes and analyzers based working with broad isotopic distributions ™ ™ on user-selected parameters, including • Thermo Scientific Xcalibur processing • Quanfirmation experiment uses synchronous precursor m/z, intensity, and/or charge. and instrument control software HR/AM SIM for quantitation with full-scan • Tune editor for system calibration, • Precursor fragmentation can take place in MS/MS in the ion trap for precursor diagnostics, and manual data acquisition the ion-routing multipole (HCD), in the ion identity confirmation trap (CID, ETD) or both (EThCD) with • Method editor with comprehensive fragment detection in the linear ion trap or Optional Application-Specific Software application-specific template library and Orbitrap mass analyzers at any stage of ™ ™ drag-and-drop user interface to facilitate • Thermo Scientific Compound Discoverer MSn analysis method development software – integrated solution for • Dynamic exclusion allows acquisition of small-molecule structural identification Operation Modes MS/MS and MSn spectra from lower-intensity • Thermo Scientific™ Mass Frontier™ • Top-speed mode maximizes the number of ion species software – predictive fragmentation high-quality MSn spectra from each cycle • Automatic gain control (AGC) ensures that software for spectral interpretation and by intelligently scheduling MS and the ion trap is always filled with the classification software to identify data-dependant MSn scans based on a optimum number of ions for any scan type unknowns user-definable time between adjacent • Normalized collision energy for both CID • Thermo Scientific™ Pinpoint™ software – survey scans and HCD fragmentation techniques comprehensive targeted protein • Synchronous MS3 precursor selection ensures reproducible data from instrument quantitation platform significantly increases the number of to instrument • Thermo Scientific™ ProSightPC™ software – peptides accurately quantified in isobaric Advanced Data-Dependent Experiments stand-alone software for analyzing mass tagging experiments top-down intact-protein data, but also • Quanfirmation experiment uses synchronous • Dynamic Scan Management facilitates a wide middle-down and bottom-up data high-resolution, accurate-mass (HR/AM) range of data-dependent experiments with ™ real-time selection, prioritization, and routing • Thermo Scientific Protein Deconvolution selected-ion monitoring (SIM) for quantitation software – intact-protein analysis platform and sensitive full-scan MS/MS in the ion of precursors to different fragmentation trap for precursor identity confirmation modes and analyzers based on user-selected • Thermo Scientific™ Discoverer™ parameters including precursor , intensity, software – flexible, expandable platform for • Parallelization maximizes the amount of m/z and/or charge the analysis of qualitative and quantitative high-quality data acquired by synchronizing proteomics data operation of the quadrupole mass filter, • Fragment ion or neutral-loss-triggered MSn ™ ™ ion-routing multipole, and ion trap and experiments including at the same MSn level • Thermo Scientific SIEVE software – Orbitrap mass analyzers • Synchronous MS3 precursor selection label-free, semi-quantitative differential significantly increases the number of analysis of highly complex data sets peptides accurately quantified in isobaric mass tagging experiments Product Specifications Product Installation Requirements Power • Instrument – 230 Vac ± 10% single phase, 15 Ampere, 50/60 Hz, with earth ground • Data system – 120 or 230 Vac single phase, 50/60 Hz, with earth ground Gas • Ultra-high-purity (99.999%) with less than 1 ppm each of water, oxygen, and total hydrocarbons • High-purity nitrogen (99.5% pure, flow rate 15 L/min) nitrogen gas supply for the ion source • Ultra-high-purity nitrogen (UHP, 99.999%) with less than one ppm each of water and oxygen for the ion-routing multipole and ETD/IC Size 1270 × 767 × 703 mm (w, d, h). The system comes mounted on a 1320 × 788 × 726 mm (w, d, h) table, similar to a standard lab bench Weight 318 kg (700 pounds) with table but without data system, vacuum rough pumps, and optional items Environment • System averages 2,800 W (10,000 Btu/hr) output • Operating environment must be 16–26 °C (59–78 °F) and relative humidity must be 50–80% with no condensation • Optimum operating temperature is 18–21 °C (65–70 °F) Figure 2. Orbitrap Fusion MS dimensions

For Research Use Only. Not for use in diagnostic procedures. www.thermoscientific.com ©2016 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization. Intel is a registered Thermo Fisher Scientific, trademark of Intel Corporation. Microsoft and Windows are registered trademarks of Microsoft Corporation. All other trademarks are the San Jose, CA USA is property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are ISO 9001:2008 Certified. available in all countries. Please consult your local sales representative for details.

Africa +43 1 333 50 34 0 Denmark +45 70 23 62 60 Japan +81 45 453 9100 Russia/CIS +43 1 333 50 34 0 Australia +61 3 9757 4300 Europe-Other +43 1 333 50 34 0 Korea +82 2 3420 8600 Singapore +65 6289 1190 Austria +43 810 282 206 Finland +358 10 3292 200 Latin America +1 561 688 8700 Spain +34 914 845 965 Belgium +32 53 73 42 41 France +33 1 60 92 48 00 Middle East +43 1 333 50 34 0 Sweden +46 8 556 468 00 Canada +1 800 530 8447 Germany +49 6103 408 1014 Netherlands +31 76 579 55 55 Switzerland +41 61 716 77 00 China 800 810 5118 (free call domestic) India +91 22 6742 9494 New Zealand +64 9 980 6700 UK +44 1442 233555 400 650 5118 Italy +39 02 950 591 Norway +46 8 556 468 00 USA +1 800 532 4752 PS63844-EN 0216S