A Review of Antimicrobial Properties of Alum and Sundry Applications
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ACTA SCIENTIFIC MICROBIOLOGY (ISSN: 2581-3226) Volume 3 Issue 4 April 2020 Review Article A Review of Antimicrobial Properties of Alum and Sundry Applications Lawrence O Amadi* Received: February 25, 2020 Department of Microbiology, Faculty of Science, Rivers State University, Nkpolu- Oroworukwo, Port Harcourt, Nigeria Published: March 10, 2020 © All rights are reserved by *Corresponding Author: Lawrence O Amadi, Department of Microbiology, Faculty Lawrence O of Science, Rivers State University, Nkpolu-Oroworukwo, Port Harcourt, Nigeria. Amadi. Abstract Potassium aluminium sulphate (PAS) commonly referred to as ‘potash alum (PA)’, ‘alum’ or ‘tawas’ has recently drawn the atten- In vitro and tion of the scientific community as an efficient, safe and eco-friendly inorganic compound, commercially available and cost effective. In vivo studies report that alum individually or in synergism have antimicrobial properties against a broad spectrum of bacterial It demonstrated a high propensity of antimicrobial activity in a variety of systems traditionally and scientifically. Several world is bedevilled with antibiotic drug abuse resulting in antimicrobial resistance (AMR). Alum, however, has found applications in and fungal species and harnesses other activities beneficial to humans. This article has become crucial especially at a time when the a wide spectrum of human activities such as pharmaceutical, cosmetic, food, textile and synthetic industries. This review presents an updateKeywords: of findings Alum; Antimicrobialof this inorganic Agent; sulphate Synergism; salt as anAntibiotic; antimicrobial Bacteria; agent Fungi and miscellaneous applications. Introduction Apart from the natural sourcing of alum, fabricated aluminum Alum occurs naturally in rocks that are located in areas where cans are currently recycled and chemically converted into an alu- sulphide materials and potassium-bearing minerals such as kalin- minium compound, hydrated PAS with an energy savings of 95% ite, alunite and leucite which on treatment with sulphuric acid re- interesting means of green reduction of environmental pollution sults in crystals of alum. Alum is a general name for a class of dou- over refining and smelting bauxite ore. However, this provides an ble sulphates containing aluminum and such metals as potassium, via conversion of waste to wealth [3]. ammonium, iron, etc. Versatility of PAS individually or in synergism as; food preser- They are commonly found in India, Egypt, - are a variety of commercially available alums; soda alum, Ammoni- Nepal, Italy, Philippines and many parts of Asian countries. There crobial agents and sundry applications had been well documented um alum, Chrome alum, Selenate alum, Alumen exsiccatum (dried vative, medicine, water purifier, biotransformational and antimi [4-6] - alum), Aluminium sulphate (also called papermaker’s alum, it is centuated by various anthropogenic activities such as heavy use not technically alum but further processing and evaporation re- . The threat of AMR organisms over the decades has been ac and abuse of antibiotics, discharge of hospital and agricultural ef- sults in alum crystals) [1]. PAS or alum is an odourless, colourless crystalline solids with chemical formula, KAl (SO4)2. Based on the amount of water molecules, these hydrates are represented by the fluents resulting in serious socioeconomic challenges worldwide. chemical formulae KAl (SO4)2.12H2O or K2SO4.Al2 (SO4)3. 24H2O [2]. substances of vegetative or mineral origin and natural products, as Thus, investigators are sourcing for other materials, such as Citation: Lawrence O Amadi. “A Review of Antimicrobial Properties of Alum and Sundry Applications". Acta Scientific Microbiology 3.4 (2020): 109-117. A Review of Antimicrobial Properties of Alum and Sundry Applications 110 alternative to conventional antimicrobial agents. One of such natu- Mi- ral products used for therapeutic purposes for centuries is white crococcus luteus Staphylococcus epidermidis alum against four malodour-producing axillary bacterial flora ( alum or PA [7]. Notable advantages for the use of alum include cost 14990), Corynebacterium xerosis Bacilus (ATCC 49732), (ATCC effectiveness, availability, nontoxicity, reusability and ecofriendli- subtilis - (ATCC BAA-1293) and ness [6] ing axillary malodour. In vitro activity using broth dilution method (ATCC 19659) as an alternative natural product for reduc visibly demonstrated over the years through the myriads of its for different concentrations (0.937 - 20.0mg/mL) on Luria-Bertani . The potency of alum as an antimicrobial agent had been broth exhibited excellent inhibitory effects on all tested bacteria. research and development. C. xerosis, at 1.88 mg/mL. M. beneficial activities and relevance in a broad spectrum of human luteus, B. subtilis and S.epidermidis showed a higher MIC of 3.75, Recently, extensive research interest in this natural product has The lowest MIC of alum was against widened and opened new horizons and frontiers of knowledge inhibited at a concentration of 7.50 mg/mL [7] 5.00 and 7.50 mg/mL, respectively. These bacteria were completely effects of alum against axillary malodour-producing bacteria could Combination strategies exploring the use of phytomedicinals with . Thus, antibacterial in drug combination therapeutic strategy by boosting its efficacy. be recommended and used either directly by topical application or natural product and/or standard antimicrobials are essentially as an active ingredient in deodorants and antiperspirants. critical for creating a truly global and effective response to treat microbial infection and ameliorate AMR. Undoubtedly, combina- Another study also, observed that white alum was potent tion strategy may improve drug performance by synergism which against Escherichia coli O157:H7 at a concentration above 1% (p may proffer a better alternative to microbial resistance single < 0.05). Such effect was dose and time dependent as well as with drug/substance as this protocol is usually more effective at low other disinfectants. Strong antibacterial activity of white alum dosage. Some major advantages of combination therapy are cost compared with control was shown against tested bacterium and effectiveness, reduction of man-hour (as new drug is neither de- could be used as an inhibitor of bacterial growth [9]. signed, formulated nor synthesized in the laboratory), less side effects associated with higher drug dosage can be minimized with- In a recent work attempt was made to provide some experi- out compromising performance. However, encouraging the use of mental proof of bioactivity of some of these G-R minerals (Lem- drugs in combination would improve the prevention and manage- - nian Earth, Samian Earth and alum), by investigating their anti below in two (2) broad headings: Antimicrobial activity of alum Gram negative (Pseudomonas aerugenosa) and one Gram positive ment of microbial infections therapy. The PAS shall be reviewed bacterial action. They were tested against specific pathogens, one and sundry applications. (Staphylococcus aureus degrees of antibacterial action with the alum group mineral being ) bacterium. The results showed varying Antimicrobial activity of alum the most effective [10]. Bacterial isolates from oysters treated with Antibacterial effect of alum PAS exhibited broad spectrum antibacterial potency against Gram Among varieties of commercially available alums, potash alum negative and Gram positive by in-vitro susceptibility tests but more is very much common both for household, pharmaceutical and - sitivity on Proteus sp (18.0mm), Bacillus subtilis and Staphylococcus medicinal applications. Alum concentrations at (4.4 - 35.0mg/ efficacious by agar well diffusion (AWD) method. High levels of sen mL) showed zones of inhibition which ranged from 0.005 - aureus (17.0mm), Escherichia coli and Klebsiella species (16.0mm) 0.438cm against E. faecalis, 0.019 - 0.421cm against E. faecium, and Pseudomonas aeruginosa and Vibrio species (13.0mm) were 0.011 - 0.455cm against S. aureus, 0.013 - 0.430cm against E. coli, demonstrated respectively [11]. and 0.025 - 0.485cm against K. pneumoniae respectively at 24hr Synergism with plant extract Synergistic effect has been shown by alum with various plant PPM alum solution as less than 1000 PPM alum or more [8]. Ad- incubation. These results corroborate previous reports of 100 extracts. A combination therapeutic strategy with aqueous extract ditionally, bactericidal effect of alum had been observed but the mechanism was not prominent. But it was postulations that it ef- fect was to reduction in acidity or deleterious effects on bacterial and alum (ALE+Alum) exhibited more beneficial antibacterial susceptibility of these pathogens to the combinations were much cell wall. Another study determined the antibacterial activities of activity on test bacteria than with methanolic (MLE+Alum). The Citation: Lawrence O Amadi. “A Review of Antimicrobial Properties of Alum and Sundry Applications". Acta Scientific Microbiology 3.4 (2020): 109-117. A Review of Antimicrobial Properties of Alum and Sundry Applications 111 more pronounced on the Gram negative bacteria thus, Pseudo- bacterial susceptibility found all isolates resistant to several an- monas aeruginosa (21mm) and Escherichia coli (20mm) than on Gram positive, Staphylococcus aureus and Bacillus subtilis (19mm) alum, inhibitory effect was highest on E. coli and C. albicans (resis- tibiotics tested and considered multi-drug resistant (MDR). With respectively