foods Article A New Rapid Method for the Authentication of Common Octopus (Octopus vulgaris) in Seafood Products Using Recombinase Polymerase Amplification (RPA) and Lateral Flow Assay (LFA) Amaya Velasco 1,*, Graciela Ramilo-Fernández 1 , Françoise Denis 2, Luís Oliveira 3 , Peter Shum 4 , Helena Silva 3 and Carmen G. Sotelo 1 1 Instituto de Investigaciones Marinas (IIM-CSIC), Eduardo Cabello 6, 36208 Pontevedra, Spain;
[email protected] (G.R.-F.);
[email protected] (C.G.S.) 2 BOREA MNHN, CNRS 8067, SU, IRD 207, UCN, UA-BIOSSE Le Mans Université, 72000 Le Mans, France;
[email protected] 3 Instituto Português do Mar e da Atmosfera (IPMA, I.P.), Av. Dr. Alfredo Magalhães Ramalho 6, 1495-165 Algés, Portugal;
[email protected] (L.O.);
[email protected] (H.S.) 4 School of Biological and Environmental Sciences, Liverpool John Moores University (LJMU), Liverpool L2 2QP, UK;
[email protected] * Correspondence:
[email protected]; Tel.: +34-986231930 Abstract: The common octopus (Octopus vulgaris) is a highly valued cephalopod species which is Citation: Velasco, A.; marketed with different grades of processing, such as frozen, cooked or even canned, and is likely Ramilo-Fernández, G.; Denis, F.; to be mislabeled. Some molecular methods have been developed for the authentication of these Oliveira, L.; Shum, P.; Silva, H.; Sotelo, products, but they are either labor-intensive and/or require specialized equipment and personnel. C.G. A New Rapid Method for the This work describes a newly designed rapid, sensitive and easy-to-use method for the detection of Authentication of Common Octopus Octopus vulgaris in food products, based on Recombinase Polymerase Amplification (RPA) and a (Octopus vulgaris) in Seafood Products detection using a Lateral Flow assay (LFA).