Retention Time Table for Carbomix Phases
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Retention time Table for Carbomix Phases Compound Carbomix H-NP5 Carbomix H-NP10 Carbomix Ca-NP5 Carbomix Ca-NP10 Carbomix Pb-NP5 Carbomix Pb-NP10 8% 5% 8% 10% 8% 5% 8% 10% 8% 5% 8% Conditions: Acetic acid 13.58 17.52 14.64 13.39 / / / / / / / Carbomix H-NP10 Adonitol 9.85 13.59 11.10 10.26 13.40 16.76 14.73 13.67 18.81 21.88 19.88 Mobile phase:2.5 mM H2SO4 Arabinitol 10.06 13.82 11.30 10.41 15.99 19.80 17.72 16.06 23.63 27.76 24.95 Flow rate:0.6 mL/min D-(-)-Arabinose 9.77 13.46 10.93 10.08 12.13 13.77 13.43 12.52 14.81 17.74 15.76 Detector:RID L-(+)-Arabinose 9.77 13.45 10.93 10.08 12.13 15.45 13.41 12.53 14.82 17.74 15.76 : 1,4-Butanediol 19.52 23.11 / 19.26 14.41 17.81 15.96 14.48 15.98 17.77 16.49 Column Tem. 80℃ n-Butanol 34.23 35.93 / 32.66 24.17 / / / 28.37 28.28 27.91 Injection Volume:10 μL (3 injections) t-Butanol 23.19 / / / 15.11 / / / 15.96 17.80 16.60 sec-butyl alcohol 28.55 31.90 30.25 28.23 18.82 22.83 21.06 19.01 20.63 22.25 21.06 Carbomix H-NP5 D-(+)-Cellobiose 7.79 9.79 8.18 8.17 8.42 10.10 8.81 8.96 10.30 12.27 10.89 Mobile phase:2.5 mM H2SO4 Citric acid 8.09 10.26 8.69 8.35 / / / / / / / Flow rate:0.6 mL/min Erythriyol 10.74 14.70 11.94 11.00 14.15 17.34 15.98 14.47 18.67 21.34 19.60 Detector:RID Ethanol 20.24 23.13 21.82 20.72 15.48 18.30 16.38 15.89 16.32 17.69 16.79 Column Tem.: 55℃ Formic acid 12.59 16.11 13.51 12.48 / / / / / / / Injection Volume:10 μL (3 injections) D-Fructose 9.25 12.65 10.27 9.58 12.08 15.27 13.34 12.45 15.85 19.17 16.86 Fumaric acid 11.71 / 13.90 / / / / / / / / Carbomix Ca-NP10 Galactitol 9.51 13.13 10.66 9.87 18.22 22.57 19.44 18.05 30.81 36.54 32.57 : D-(+)-Galactose 9.13 12.54 10.15 9.48 10.73 13.77 11.77 11.20 13.54 16.50 14.52 Mobile phase H2O D-Glucose 8.76 11.90 9.68 9.16 9.82 12.37 10.61 10.32 11.97 14.31 12.74 Flow rate:0.6 mL/min Glycerol 12.19 16.07 13.61 12.46 14.96 17.89 16.20 15.12 18.34 20.59 19.13 Detector:RID IPA 21.94 25.79 23.89 22.53 15.37 18.61 17.16 15.88 16.27 17.86 16.78 Column Tem.: 85℃ Lactic acid 11.40 15.41 12.70 11.55 / / / / / / / Injection Volume:10 μL (3 injections) B-Lactose 7.93 10.24 8.42 8.29 8.70 10.81 9.25 9.22 10.92 13.51 11.70 D-Lyxose 9.50 13.08 10.64 9.87 12.65 16.05 13.96 13.02 15.35 18.33 16.33 Carbomix Ca-NP5 Maleic acid 8.42 9.50 8.53 8.56 / / / / / / / Mobile phase: H2O Malic acid 9.03 12.03 9.80 9.24 / / / / / / / Flow rate:0.6 mL/min Maltotetraose 7.42 / / / 7.78 / / / 9.40 12.11 10.31 Detector:RID Maltitol 8.04 10.51 8.41 8.29 10.77 14.74 11.92 11.24 16.69 22.68 18.44 Column Tem.: 85℃ D-(+)-Maltose 7.85 10.01 8.29 8.23 8.56 10.43 9.02 9.09 10.79 13.04 11.47 : Maltotriose 7.54 8.94 7.70 7.90 8.08 9.40 8.33 8.57 10.23 12.49 10.87 Injection Volume 10 μL (3 injections) D-Mannitol 9.40 12.99 10.53 9.79 15.54 19.57 17.34 15.60 23.24 28.05 24.89 D-(+)-Mannose 9.11 12.55 10.13 9.48 11.00 14.09 12.05 11.45 15.01 18.43 16.09 Carbomix Pb-NP10 D-(+)-Melezitose 7.55 / / / 7.99 9.14 8.18 8.49 9.43 11.23 10.00 Mobile phase: H2O Methanol 17.99 20.60 19.21 18.28 15.32 17.74 16.08 15.64 15.82 17.19 16.31 Flow rate:0.6 mL/min Oxalic acid : 7.61 7.72 7.44 7.72 / / / / / / / Detector RID dihydrate Column Tem.: 75℃ 1-Propanol 25.83 27.83 26.56 25.16 19.08 21.58 20.44 18.95 21.40 21.48 21.21 Injection Volume:10 μL (3 injections) 1,2-Propylene 15.43 / / / 16.23 / / / 19.68 20.88 20.10 glycol Carbomix Pb-NP5 Pyruvic acid 8.99 10.72 10.37 9.24 / / / / / / / : D-(-)-Ribose 9.95 13.73 11.16 10.25 19.37 24.21 20.70 19.23 29.79 35.80 31.07 Mobile phase H2O D-Sorbitol 9.49 13.12 10.64 9.86 18.92 23.74 20.22 18.71 32.87 39.86 34.96 Flow rate:0.6 mL/min Succinic acid 10.46 14.45 11.54 10.47 / / / / / / / Detector:RID D-(+)-Sucrose / / / / 8.49 10.31 8.93 9.03 10.34 12.38 10.97 Column Tem.:75℃ Tartartic 8.41 10.74 8.94 8.64 / / / / / / / Injection Volume:10 μL (3 injections) Triethylene glycol 14.42 19.60 16.12 14.17 17.51 20.01 17.51 17.21 35.95 34.98 34.53 Xylitol 10.21 14.03 11.47 10.53 18.79 23.17 21.08 18.66 30.06 35.71 31.75 D-Xylose 9.19 12.61 10.24 9.60 10.72 13.49 11.63 11.19 12.93 15.24 13.70 www.sepax-tech.com Toll Free: 1-877-SEPAX-US CC1027 Mixed polysaccharides Analysis on Carbomix Pb-NP5:8% ( 7815 ) Column: Carbomix Pb-NP5:8% ( 5 µm, 8%, 7.8 x 150 mm ) Mobile phase: Water, Flow rate: 0.6 mL/min, Detector: RI ( 35 ℃ ) Column temperature: 80 ℃, Injection volume: 10 µL Samples: Cellobiose + Glucose + Mannose ( 5.0 mg/mL ), Pressure: 10 bar nRIU 6.842 40000 6.056 8.223 30000 20000 10000 0 0 2 4 6 8 10 12 min Cellobiose Glucose Mannose CC1027 Mixed polysaccharides Analysis on Carbomix Pb-NP5:8% ( 7815 ) Column: Carbomix Pb-NP5:8% ( 5 µm, 8%, 7.8 x 150 mm ) Mobile phase: Water, Flow rate: 0.6 mL/min, Detector: RI ( 35 ℃ ) Column temperature: 80 ℃, Injection volume: 10 µL Samples: Cellobiose + Glucose + Xylose + Arabinose ( 5.0 mg/mL ) Pressure: 10 bar nRIU 8.110 6.841 7.268 40000 6.053 30000 20000 10000 0 0 2 4 6 8 10 12 min Cellobiose Glucose Xylose Arabinose CC1027 Mixed polysaccharides Analysis on Carbomix Pb-NP5:8% ( 7815 ) Column: Carbomix Pb-NP5:8% ( 5 µm, 8%, 7.8 x 150 mm ) Mobile phase: Water, Flow rate: 0.6 mL/min, Detector: RI ( 35 ℃ ) Column temperature: 80 ℃, Injection volume: 10 µL Samples: Cellobiose + Glucose + Mannose + Xylose + Arabinose ( 5.0 mg/mL ) Pressure: 10 bar nRIU 60000 Xylose 50000 Mannose Arabinose Glucose Cellobiose 40000 30000 20000 10000 0 2 4 6 8 10 12 min Cellobiose Glucose Xylose Arabinose/ Mannose CC1027 Mixed polysaccharides Analysis on Carbomix Pb-NP5:8% ( 7830 ) Column: Carbomix Pb-NP5:8% ( 5 µm, 8%, 7.8 x 300 mm ) Mobile phase: Water, Flow rate: 0.6 mL/min, Detector: RI ( 35 ℃ ), Column temperature: 80 ℃ Injection volume: 10 µL, Samples: Cellobiose + Glucose + Mannose ( 5.0 mg/mL ), Pressure: 27 bar nRIU 25000 11.794 10.053 20000 15.066 15000 10000 5000 0 0 2.5 5 7.5 10 12.5 15 17.5 min Cellobiose Glucose Mannose CC1027 Mixed polysaccharides Analysis on Carbomix Pb-NP5:8% ( 7830 ) Column: Carbomix Pb-NP5:8% ( 5 µm, 8%, 7.8 x 300 mm ) Mobile phase: Water, Flow rate: 0.6 mL/min, Detector: RI ( 35 ℃ ), Column temperature: 80 ℃ Injection volume: 10 µL, Samples: Cellobiose + Glucose + Xylose + Arabinose ( 5.0 mg/mL ) Pressure: 27 bar nRIU 12.796 14.681 11.797 30000 Xylose Glucose Arabinose 10.056 Cellobiose 20000 10000 0 0 2 4 6 8 10 12 14 16 18 min Cellobiose Glucose Xylose Arabinose CC1027 Mixed polysaccharides Analysis on Carbomix Pb-NP5:8% ( 7830 ) Column: Carbomix Pb-NP5:8% ( 5 µm, 8%, 7.8 x 300 mm ) Mobile phase: Water, Flow rate: 0.6 mL/min, Detector: RI ( 35 ℃ ), Column temperature: 80 ℃ Injection volume: 10 µL, Samples: Cellobiose + Glucose + Mannose + Xylose + Arabinose ( 5.0 mg/mL ) Pressure: 27 bar nRIU 12.793 14.703 Xylose Glucose Mannose Arabinose 30000 Cellobiose 11.794 10.054 20000 10000 0 0 2 4 6 8 10 12 14 16 18 min Cellobiose Glucose Xylose Arabinose /Mannose Tremella polysaccharide analysis on Zenix ( 7830 ) ZC1001 Column: Zenix-100 ( 3 µm, 100 Å, 7.8 x 300 mm), Zenix-80 ( 3 µm, 80 Å, 7.8 x 300 mm) Mobile phase: 0.7% Na2SO4 , Flow rate: 0.5 mL/min Detector: RI ( 35℃ ), Column temperature: 35℃, Injection volume: 10 µL Sample: 5 mg/mL Tremella polysaccharide in mobile phase nRIU 7000 10kD-100kD 6000 5000 4000 3000 Zenix-100 2000 1000 Zenix-80 0 0 5 10 15 20 25 min www.sepax-tech.com 1 Better Surface Chemistry for Better Separation © Sepax Technologies, Inc.