DOCSLIB.ORG

Diversity of Cultured Isolates and Field Populations of The

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Diversity of Cultured Isolates and Field Populations of The Diversity of cultured isolates and field populations of the arbuscular mycorrhizal fungus Glomus intraradices: Development and application of molecular detection methods for mitochondrial haplotypes Inauguraldissertation zur Erlangung der Würde eines Doktors der Philosophie vorgelegt der Philosophisch-Naturwissenschaftlichen Fakultät der Universität Basel von Boris Börstler aus Braunschweig, Deutschland Basel, 2010 Genehmigt von der Philosophisch-Naturwissenschaftlichen Fakultät auf Antrag von Prof. Dr. Thomas Boller, Prof. Dr. Dirk Redecker, Prof. Dr. Andres Wiemken Basel, den 22.06.2010 Prof. Dr. Eberhard Parlow, Dekan Table of contents Summary .............................................................................................................................. 1 Chapter 1: General introduction........................................................................................ 3 1.1 Mycorrhizas – origin, evolution and types ............................................................................... 3 1.2 The arbuscular mycorrhiza (AM) ............................................................................................. 7 1.2.1 Phylogeny and taxonomy of the Glomeromycota ............................................................. 7 1.2.2 Species concept and genetics of AM fungi (AMF) ......................................................... 12 1.2.3 Structural features of the AM and life cycle of AMF ..................................................... 13 1.2.4 AM partnerships: benefits and ecology ........................................................................... 17 1.2.4.1 Extent of plant – AMF associations in the plant kingdom ....................................... 17 1.2.4.2 Functional aspects of AM associations .................................................................... 19 1.2.4.3 Multitrophic interactions .......................................................................................... 22 1.2.4.4 Other ecological aspects: AMF and ecosystem functioning .................................... 23 1.2.5 Biodiversity studies of AMF communities and populations ........................................... 25 1.2.5.1 Different strategies and methods for the detection of AMF in the field................... 25 1.2.5.2 Marker genes for AMF community studies ............................................................. 26 1.2.5.3 Fingerprinting methods and quantitative approaches ............................................... 28 1.2.5.4 Marker genes available for the “species” or intraspecific level of AMF ................. 29 1.2.5.5 AMF communities in different environments .......................................................... 30 1.2.5.6 The first studies of intraspecific gene structure of AMF species in field settings .... 33 1.3 Mitochondria in AMF ............................................................................................................ 34 1.3.1 Mitochondrial genome structure and inheritance ............................................................ 34 1.3.2 The use of mitochondrial genes in phylogenetic analyses or as molecular markers ....... 36 1.4 Aims of this thesis .................................................................................................................. 38 Chapter 2: Genetic diversity of the arbuscular mycorrhizal fungus Glomus intraradices as determined by mitochondrial large subunit rRNA gene sequences is considerably higher than previously expected ................................................................ 41 2.1 Summary ................................................................................................................................ 42 2.2 Introduction ............................................................................................................................ 42 2.3 Materials and Methods ........................................................................................................... 45 2.3.1 Root organ cultures of G. intraradices ............................................................................ 45 2.3.2 Inocula and pot cultures of G. intraradices ..................................................................... 45 2.3.3 Field-collected root samples ............................................................................................ 46 2.3.4 DNA extraction ............................................................................................................... 46 2.3.5 PCR amplification of mtLSU .......................................................................................... 47 i Table of contents 2.3.6 PCR amplification of nuclear-encoded ITS rDNA ......................................................... 49 2.3.7 Cloning, sequencing and sequence analyses ................................................................... 50 2.3.8 Phylogenetic analyses ..................................................................................................... 50 2.3.9 RFLP analyses ................................................................................................................. 51 2.4 Results .................................................................................................................................... 52 2.4.1 Diversity of ITS sequences and mtLSU haplotypes in G. intraradices isolates ............. 52 2.4.2 MtLSU exon and intron phylogeny ................................................................................. 54 2.4.3 Intron stability in the mtLSU of G. intraradices ............................................................. 58 2.4.4 Detection of haplotypes in field-collected roots .............................................................. 58 2.4.5 RFLP analyses ................................................................................................................. 58 2.5 Discussion .............................................................................................................................. 60 2.6 Acknowledgements ................................................................................................................ 63 Chapter 3: Diversity of mitochondrial large subunit rDNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi-natural grasslands . 65 3.1 Abstract .................................................................................................................................. 66 3.2 Introduction ............................................................................................................................ 66 3.3 Materials and methods ........................................................................................................... 69 3.3.1 Field sites and sampling .................................................................................................. 69 3.3.2 DNA extraction ............................................................................................................... 71 3.3.3 PCR amplification of mtLSU .......................................................................................... 71 3.3.4 Specific PCR amplification of mtLSU haplotype I ......................................................... 72 3.3.5 RFLP analyses and definition of RFLP types ................................................................. 73 3.3.6 Sequencing, cloning and sequence analyses ................................................................... 74 3.3.7 Phylogenetic analyses ..................................................................................................... 77 3.3.8 Population genetics ......................................................................................................... 77 3.3.9 Statistical analyses ........................................................................................................... 77 3.4 Results .................................................................................................................................... 78 3.4.1 Amplification success ..................................................................................................... 78 3.4.2 RFLP analyses ................................................................................................................. 80 3.4.3 Sequence-based analyses ................................................................................................. 85 3.4.4 Phylogenetic analyses ..................................................................................................... 89 3.4.5 The specific approach to detect haplotype I .................................................................... 89 3.5 Discussion .............................................................................................................................. 90 3.6 Acknowledgements ................................................................................................................ 93 ii Table of contents Chapter 4: Development of specific mitochondral markers for the Glomus intraradices isolate BEG140 .............................................................................................. 95 4.1 Introduction ............................................................................................................................ 95 4.2 Characterization of mitochondrial haplotypes of BEG140 .................................................... 95 4.2.1 Materials and Methods ...................................................................................................
Load more

Recommended publications
  • The Obligate Endobacteria of Arbuscular Mycorrhizal Fungi Are Ancient Heritable Components Related to the Mollicutes
    The ISME Journal (2010) 4, 862–871 & 2010 International Society for Microbial Ecology All rights reserved 1751-7362/10 $32.00 www.nature.com/ismej ORIGINAL ARTICLE The obligate endobacteria of arbuscular mycorrhizal fungi are ancient heritable components related to the Mollicutes Maria Naumann1,2, Arthur Schu¨ ler2 and Paola Bonfante1 1Department of Plant Biology, University of Turin and IPP-CNR, Turin, Italy and 2Department of Biology, Inst. Genetics, University of Munich (LMU), Planegg-Martinsried, Germany Arbuscular mycorrhizal fungi (AMF) have been symbionts of land plants for at least 450 Myr. It is known that some AMF host in their cytoplasm Gram-positive endobacteria called bacterium-like organisms (BLOs), of unknown phylogenetic origin. In this study, an extensive inventory of 28 cultured AMF, from diverse evolutionary lineages and four continents, indicated that most of the AMF species investigated possess BLOs. Analyzing the 16S ribosomal DNA (rDNA) as a phylogenetic marker revealed that BLO sequences from divergent lineages all clustered in a well- supported monophyletic clade. Unexpectedly, the cell-walled BLOs were shown to likely represent a sister clade of the Mycoplasmatales and Entomoplasmatales, within the Mollicutes, whose members are lacking cell walls and show symbiotic or parasitic lifestyles. Perhaps BLOs maintained the Gram-positive trait whereas the sister groups lost it. The intracellular location of BLOs was revealed by fluorescent in situ hybridization (FISH), and confirmed by pyrosequencing. BLO DNA could only be amplified from AMF spores and not from spore washings. As highly divergent BLO sequences were found within individual fungal spores, amplicon libraries derived from Glomus etunicatum isolates from different geographic regions were pyrosequenced; they revealed distinct sequence compositions in different isolates.
    [Show full text]
  • Fungal Evolution: Major Ecological Adaptations and Evolutionary Transitions
    Biol. Rev. (2019), pp. 000–000. 1 doi: 10.1111/brv.12510 Fungal evolution: major ecological adaptations and evolutionary transitions Miguel A. Naranjo-Ortiz1 and Toni Gabaldon´ 1,2,3∗ 1Department of Genomics and Bioinformatics, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain 2 Department of Experimental and Health Sciences, Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain 3ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain ABSTRACT Fungi are a highly diverse group of heterotrophic eukaryotes characterized by the absence of phagotrophy and the presence of a chitinous cell wall. While unicellular fungi are far from rare, part of the evolutionary success of the group resides in their ability to grow indefinitely as a cylindrical multinucleated cell (hypha). Armed with these morphological traits and with an extremely high metabolical diversity, fungi have conquered numerous ecological niches and have shaped a whole world of interactions with other living organisms. Herein we survey the main evolutionary and ecological processes that have guided fungal diversity. We will first review the ecology and evolution of the zoosporic lineages and the process of terrestrialization, as one of the major evolutionary transitions in this kingdom. Several plausible scenarios have been proposed for fungal terrestralization and we here propose a new scenario, which considers icy environments as a transitory niche between water and emerged land. We then focus on exploring the main ecological relationships of Fungi with other organisms (other fungi, protozoans, animals and plants), as well as the origin of adaptations to certain specialized ecological niches within the group (lichens, black fungi and yeasts).
    [Show full text]
  • Bodenmikrobiologie (Version: 07/2019)
    Langzeitmonitoring von Ökosystemprozessen - Methoden-Handbuch Modul 04: Bodenmikrobiologie (Version: 07/2019) www.hohetauern.at Impressum Impressum Für den Inhalt verantwortlich: Dr. Fernando Fernández Mendoza & Prof. Mag Dr. Martin Grube Institut für Biologie, Bereich Pflanzenwissenschaften, Universität Graz, Holteigasse 6, 8010 Graz Nationalparkrat Hohe Tauern, Kirchplatz 2, 9971 Matrei i.O. Titelbild: Ein Transekt im Untersuchungsgebiet Innergschlöss (2350 m üNN) wird im Jahr 2017 beprobt. © Newesely Zitiervorschlag: Fernández Mendoza F, Grube M (2019) Langzeitmonitoring von Ökosystemprozessen im Nationalpark Hohe Tauern. Modul 04: Mikrobiologie. Methoden-Handbuch. Verlag der Österreichischen Akademie der Wissenschaften, Wien. ISBN-Online: 978-3-7001-8752-3, doi: 10.1553/GCP_LZM_NPHT_Modul04 Weblink: https://verlag.oeaw.ac.at und http://www.parcs.at/npht/mmd_fullentry.php?docu_id=38612 Inhaltsverzeichnis Zielsetzung ...................................................................................................................................................... 1 Inhalt Vorbereitungsarbeit und benötigtes Material ................................................................................................... 2 a. Materialien für die Probenahme und Probenaufbewahrung ................................................................ 2 b. Materialien und Geräte für die Laboranalyse ...................................................................................... 2 Arbeitsablauf ...................................................................................................................................................
    [Show full text]
  • Checklist of the Species of the Genus Tricholoma (Agaricales, Agaricomycetes) in Estonia
    Folia Cryptog. Estonica, Fasc. 47: 27–36 (2010) Checklist of the species of the genus Tricholoma (Agaricales, Agaricomycetes) in Estonia Kuulo Kalamees Institute of Ecology and Earth Sciences, University of Tartu, 40 Lai St. 51005, Tartu, Estonia. Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, 181 Riia St., 51014 Tartu, Estonia E-mail: [email protected] Abstract: 42 species of genus Tricholoma (Agaricales, Agaricomycetes) have been recorded in Estonia. A checklist of these species with ecological, phenological and distribution data is presented. Kokkukvõte: Perekonna Tricholoma (Agaricales, Agaricomycetes) liigid Eestis Esitatakse kriitiline nimestik koos ökoloogiliste, fenoloogiliste ja levikuliste andmetega heiniku perekonna (Tricholoma) 42 liigi (Agaricales, Agaricomycetes) kohta Eestis. INTRODUCTION The present checklist contains 42 Tricholoma This checklist also provides data on the ecol- species recorded in Estonia. All the species in- ogy, phenology and occurrence of the species cluded (except T. gausapatum) correspond to the in Estonia (see also Kalamees, 1980a, 1980b, species conceptions established by Christensen 1982, 2000, 2001b, Kalamees & Liiv, 2005, and Heilmann-Clausen (2008) and have been 2008). The following data are presented on each proved by relevant exsiccates in the mycothecas taxon: (1) the Latin name with a reference to the TAAM of the Institute of Agricultural and Envi- initial source; (2) most important synonyms; (3) ronmental Sciences of the Estonian University reference to most important and representative of Life Sciences or TU of the Natural History pictures (iconography) in the mycological litera- Museum of the Tartu University. In this paper ture used in identifying Estonian species; (4) T. gausapatum is understand in accordance with data on the ecology, phenology and distribution; Huijsman, 1968 and Bon, 1991.
    [Show full text]
  • Funghi Campania
    Università degli Studi di Napoli “Federico II” Dipartimento di Arboricoltura, Botanica e Patologia vegetale I funghi della Campania Emmanuele Roca, Lello Capano, Fabrizio Marziano Coordinamento editoriale: Michele Bianco, Italo Santangelo Progetto grafico: Maurizio Cinque, Pasquale Ascione Testi: Emmanuele Roca, Lello Capano, Fabrizio Marziano Coordinamento fotografico: Lello Capano Collaborazione: Gennaro Casato Segreteria: Maria Raffaela Rizzo Iniziativa assunta nell’ambito del Progetto CRAA “Azioni integrate per lo sviluppo razionale della funghicol- tura in Campania”; Coordinatore scientifico Prof.ssa Marisa Di Matteo. Foto di copertina: Amanita phalloides (Fr.) Link A Umberto Violante (1937-2001) Micologo della Scuola Partenopea I funghi della Campania Indice Presentazione........................................................................................... pag. 7 Prefazione................................................................................................ pag. 9 1 Campania terra di funghi, cercatori e studiosi....................................... pag. 11 2 Elementi di biologia e morfologia.......................................................... pag. 23 3 Principi di classificazione e tecniche di determinazione....................... pag. 39 4 Elenco delle specie presenti in Campania.............................................. pag. 67 5 Schede descrittive delle principali specie.............................................. pag. 89 6 Glossario...............................................................................................
    [Show full text]
  • Occurrence of Glomeromycota Species in Aquatic Habitats: a Global Overview
    Occurrence of Glomeromycota species in aquatic habitats: a global overview MARIANA BESSA DE QUEIROZ1, KHADIJA JOBIM1, XOCHITL MARGARITO VISTA1, JULIANA APARECIDA SOUZA LEROY1, STEPHANIA RUTH BASÍLIO SILVA GOMES2, BRUNO TOMIO GOTO3 1 Programa de Pós-Graduação em Sistemática e Evolução, 2 Curso de Ciências Biológicas, and 3 Departamento de Botânica e Zoologia, Universidade Federal do Rio Grande do Norte, Campus Universitário, 59072-970, Natal, RN, Brazil * CORRESPONDENCE TO: [email protected] ABSTRACT — Arbuscular mycorrhizal fungi (AMF) are recognized in terrestrial and aquatic ecosystems. The latter, however, have received little attention from the scientific community and, consequently, are poorly known in terms of occurrence and distribution of this group of fungi. This paper provides a global list on AMF species inhabiting aquatic ecosystems reported so far by scientific community (lotic and lentic freshwater, mangroves, and wetlands). A total of 82 species belonging to 5 orders, 11 families, and 22 genera were reported in 8 countries. Lentic ecosystems have greater species richness. Most studies of the occurrence of AMF in aquatic ecosystems were conducted in the United States and India, which constitute 45% and 78% reports coming from temperate and tropical regions, respectively. KEY WORDS — checklist, flooded areas, mycorrhiza, taxonomy Introduction Aquatic ecosystems comprise about 77% of the planet surface (Rebouças 2006) and encompass a diversity of habitats favorable to many species from marine (ocean), transitional estuaries to continental (wetlands, lentic and lotic) environments (Reddy et al. 2018). Despite this territorial representativeness and biodiversity already recorded, there are gaps when considering certain types of organisms, e.g. fungi. Fungi are considered a common and important component of almost all trophic levels.
    [Show full text]
  • Tricholoma (Fr.) Staude in the Aegean Region of Turkey
    Turkish Journal of Botany Turk J Bot (2019) 43: 817-830 http://journals.tubitak.gov.tr/botany/ © TÜBİTAK Research Article doi:10.3906/bot-1812-52 Tricholoma (Fr.) Staude in the Aegean region of Turkey İsmail ŞEN*, Hakan ALLI Department of Biology, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkey Received: 24.12.2018 Accepted/Published Online: 30.07.2019 Final Version: 21.11.2019 Abstract: The Tricholoma biodiversity of the Aegean region of Turkey has been determined and reported in this study. As a consequence of field and laboratory studies, 31 Tricholoma species have been identified, and five of them (T. filamentosum, T. frondosae, T. quercetorum, T. rufenum, and T. sudum) have been reported for the first time from Turkey. The identification key of the determined taxa is given with this study. Key words: Tricholoma, biodiversity, identification key, Aegean region, Turkey 1. Introduction & Intini (this species, called “sedir mantarı”, is collected by Tricholoma (Fr.) Staude is one of the classic genera of local people for both its gastronomic and financial value) Agaricales, and more than 1200 members of this genus and T. virgatum var. fulvoumbonatum E. Sesli, Contu & were globally recorded in Index Fungorum to date (www. Vizzini (Intini et al., 2003; Vizzini et al., 2015). Additionally, indexfungorum.org, access date 23 April 2018), but many Heilmann-Clausen et al. (2017) described Tricholoma of them are placed in other genera such as Lepista (Fr.) ilkkae Mort. Chr., Heilm.-Claus., Ryman & N. Bergius as W.G. Sm., Melanoleuca Pat., and Lyophyllum P. Karst. a new species and they reported that this species grows in (Christensen and Heilmann-Clausen, 2013).
    [Show full text]
  • Arbuscular Mycorrhizal Fungi (AMF) Communities Associated with Cowpea in Two Ecological Site Conditions in Senegal
    Vol. 9(21), pp. 1409-1418, 27 May, 2015 DOI: 10.5897/AJMR2015.7472 Article Number: 8E4CFF553277 ISSN 1996-0808 African Journal of Microbiology Research Copyright © 2015 Author(s) retain the copyright of this article http://www.academicjournals.org/AJMR Full Length Research Paper Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal Ibou Diop1,2*, Fatou Ndoye1,2, Aboubacry Kane1,2, Tatiana Krasova-Wade2, Alessandra Pontiroli3, Francis A Do Rego2, Kandioura Noba1 and Yves Prin3 1Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Sénégal. 2IRD, Laboratoire Commun de Microbiologie (LCM/IRD/ISRA/UCAD), Bel-Air BP 1386, CP 18524, Dakar, Sénégal. 3CIRAD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), TA A-82 / J, 34398 Montpellier Cedex 5, France. Received 10 March, 2015; Accepted 5 May, 2015 The objective of this study was to characterize the diversity of arbuscular mycorrhizal fungal (AMF) communities colonizing the roots of Vigna unguiculata (L.) plants cultivated in two different sites in Senegal. Roots of cowpea plants and soil samples were collected from two fields (Ngothie and Diokoul) in the rural community of Dya (Senegal). Microscopic observations of the stained roots indicated a high colonization rate in roots from Ngothie site as compared to those from Diokoul site. The partial small subunit of ribosomal DNA genes was amplified from the genomic DNA extracted from these roots by polymerase chain reaction (PCR) with the universal primer NS31 and a fungal-specific primer AML2. Nucleotide sequence analysis revealed that 22 sequences from Ngothie site and only four sequences from Diokoul site were close to those of known arbuscular mycorrhizal fungi.
    [Show full text]
  • The Revised Classification of Eukaryotes
    See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/231610049 The Revised Classification of Eukaryotes Article in Journal of Eukaryotic Microbiology · September 2012 DOI: 10.1111/j.1550-7408.2012.00644.x · Source: PubMed CITATIONS READS 961 2,825 25 authors, including: Sina M Adl Alastair Simpson University of Saskatchewan Dalhousie University 118 PUBLICATIONS 8,522 CITATIONS 264 PUBLICATIONS 10,739 CITATIONS SEE PROFILE SEE PROFILE Christopher E Lane David Bass University of Rhode Island Natural History Museum, London 82 PUBLICATIONS 6,233 CITATIONS 464 PUBLICATIONS 7,765 CITATIONS SEE PROFILE SEE PROFILE Some of the authors of this publication are also working on these related projects: Biodiversity and ecology of soil taste amoeba View project Predator control of diversity View project All content following this page was uploaded by Smirnov Alexey on 25 October 2017. The user has requested enhancement of the downloaded file. The Journal of Published by the International Society of Eukaryotic Microbiology Protistologists J. Eukaryot. Microbiol., 59(5), 2012 pp. 429–493 © 2012 The Author(s) Journal of Eukaryotic Microbiology © 2012 International Society of Protistologists DOI: 10.1111/j.1550-7408.2012.00644.x The Revised Classification of Eukaryotes SINA M. ADL,a,b ALASTAIR G. B. SIMPSON,b CHRISTOPHER E. LANE,c JULIUS LUKESˇ,d DAVID BASS,e SAMUEL S. BOWSER,f MATTHEW W. BROWN,g FABIEN BURKI,h MICAH DUNTHORN,i VLADIMIR HAMPL,j AARON HEISS,b MONA HOPPENRATH,k ENRIQUE LARA,l LINE LE GALL,m DENIS H. LYNN,n,1 HILARY MCMANUS,o EDWARD A. D.
    [Show full text]
  • Downloaded from by IP: 199.133.24.106 On: Mon, 18 Sep 2017 10:43:32 Spatafora Et Al
    UC Riverside UC Riverside Previously Published Works Title The Fungal Tree of Life: from Molecular Systematics to Genome-Scale Phylogenies. Permalink https://escholarship.org/uc/item/4485m01m Journal Microbiology spectrum, 5(5) ISSN 2165-0497 Authors Spatafora, Joseph W Aime, M Catherine Grigoriev, Igor V et al. Publication Date 2017-09-01 DOI 10.1128/microbiolspec.funk-0053-2016 License https://creativecommons.org/licenses/by-nc-nd/4.0/ 4.0 Peer reviewed eScholarship.org Powered by the California Digital Library University of California The Fungal Tree of Life: from Molecular Systematics to Genome-Scale Phylogenies JOSEPH W. SPATAFORA,1 M. CATHERINE AIME,2 IGOR V. GRIGORIEV,3 FRANCIS MARTIN,4 JASON E. STAJICH,5 and MEREDITH BLACKWELL6 1Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331; 2Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907; 3U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA 94598; 4Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1136 Interactions Arbres/Microorganismes, Laboratoire d’Excellence Recherches Avancés sur la Biologie de l’Arbre et les Ecosystèmes Forestiers (ARBRE), Centre INRA-Lorraine, 54280 Champenoux, France; 5Department of Plant Pathology and Microbiology and Institute for Integrative Genome Biology, University of California–Riverside, Riverside, CA 92521; 6Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803 and Department of Biological Sciences, University of South Carolina, Columbia, SC 29208 ABSTRACT The kingdom Fungi is one of the more diverse INTRODUCTION clades of eukaryotes in terrestrial ecosystems, where they In 1996 the genome of Saccharomyces cerevisiae was provide numerous ecological services ranging from published and marked the beginning of a new era in decomposition of organic matter and nutrient cycling to beneficial and antagonistic associations with plants and fungal biology (1).
    [Show full text]
  • How to Cite Complete Issue More Information About This Article
    Revista mexicana de biodiversidad ISSN: 1870-3453 ISSN: 2007-8706 Instituto de Biología Álvarez-Lopeztello, Jonás; Hernández-Cuevas, Laura V.; Castillo, Rafael F. del; Robles, Celerino Second world record of Glomus trufemii (Glomeromycota: Fungi), an arbuscular mycorrhizal fungus from a Mexican savanna Revista mexicana de biodiversidad, vol. 89, no. 1, 2018, pp. 298-300 Instituto de Biología DOI: 10.22201/ib.20078706e.2018.1.2101 Available in: http://www.redalyc.org/articulo.oa?id=42559253025 How to cite Complete issue Scientific Information System Redalyc More information about this article Network of Scientific Journals from Latin America and the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Project academic non-profit, developed under the open access initiative Revista Mexicana de Biodiversidad 89 (2018): 298-300 Research note Second world record of Glomus trufemii (Glomeromycota: Fungi), an arbuscular mycorrhizal fungus from a Mexican savanna Segundo registro mundial de Glomus trufemii (Glomeromycota: Fungi), un hongo micorrízico arbuscular de una sabana mexicana Jonás Álvarez-Lopeztello a, Laura V. Hernández-Cuevas b, *, Rafael F. del Castillo a, Celerino Robles a a Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, Oaxaca, Instituto Politécnico Nacional, Hornos 1003, 71230 Santa Cruz Xoxocotlán, Oaxaca, Mexico b Centro de Investigación en Genética y Ambiente, Universidad Autónoma de Tlaxcala, Km 10.5 Autopista Texmelucan-Tlaxcala, 90120 Ixtacuixtla, Tlaxcala, Mexico *Corresponding author: [email protected] (L.V. Hernández-Cuevas) Received: 25 January 2017; accepted: 07 September 2017 Abstract In Mexico, studies of diversity of arbuscular mycorrhizal fungi (AMF) are still scarce. Here we report the second record in the world, and the first record in Mexico of Glomus trufemii (Glomeraceae) from a tropical humid savanna.
    [Show full text]
  • Long-Term Preservation of Arbuscular Mycorrhizal Fungi
    Université catholique de Louvain Faculté d’ingénierie biologique, agronomique et environnementale Earth and Life Institute Pole of Applied Microbiology (ELIM) Laboratory of Mycology Long-term preservation of Arbuscular mycorrhizal fungi Thèse de doctorat présentée en vue de l’obtention du grade de Docteur en Sciences agronomiques et ingénierie biologique Ismahen Lalaymia Promoteurs: Prof. Stéphane Declerck (UCL, Belgique) Dr. Sylvie Cranenbrouck (UCL, Belgique) 2013 Université catholique de Louvain Faculté d’ingénierie biologique, agronomique et environnementale Earth and Life Institute Pole of Applied Microbiology (ELIM) Laboratory of Mycology Long-term preservation of Arbuscular mycorrhizal fungi Thèse de doctorat présentée en vue de l’obtention du grade de Docteur en Sciences agronomiques et ingénierie biologique Ismahen Lalaymia Promoteurs : Prof. S. Declerck (UCL, Belgique) Dr. S. Cranenbrouck (UCL, Belgique) Membres du Jury : Prof. Y. Larondelle (UCL, Belgique), Président Prof. A. Legreve (UCL, Belgique) Prof. P. de Vos (UGent, Belgique) Dr. B. Panis (KUL, Belgique) Louvain-La-Neuve, 2013 Acknowledgements First and foremost, I would like to express my deep gratitude to my promoter, Professor Stéphane Declerck, for the opportunity he gave me to accomplish this PhD. Thank you for guidance, enthusiastic supervision, your confidence in me and the useful critiques of this research work. I am grateful to Dr. Sylvie Cranenbrouck. Thank you Sylvie for your continuous encouragements and for the numerous stimulating discussions. Without your knowledge and help this study would not have been successful. I am thankful to the European Community for financing of the VALORAM project and for providing the financial means and laboratory facilities to complete this project. Thanks are also addressed to the people involved in the VALORAM project.
    [Show full text]