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Proceedings of The 2nd Global COE Program Symposium of Kinki University, 2009 “Sustainable Aquaculture of the Bluefin and Yellowfin Tuna – Closing the Life Cycle for Commercial Production” Edited by Dr Geoff Allan Dr Mark Booth Dr Graham Mair Mr Steven Clarke Dr Amal Biswas ISBN 978 1 74256 021 2 TABLE OF CONTENTS TABLE OF CONTENTS .......................................................................................................................4 PREFACE ...............................................................................................................................................6 ORAL PRESENTATIONS....................................................................................................................8 INTRODUCTION OF SELECTIVE BREEDING OF PACIFIC BLUEFIN TUNA Thunnus orientalis AND ITS MOLECULAR BIOLOGICAL APPROACHES..........................................................8 Yasuo Agawa, Tomoki Honroyo, Michio Kurata, Tokihiko Okada and Yoshifumi Sawada DEVELOPMENTS IN BLACKFIN TUNA Thunnus atlanticus AQUACULTURE ............................12 Daniel Benetti, John Steiglitz, Ronald Hoenig, Aaron Welch, Patrick Brown, Bruno Sardenberg and Sasa Miralao TOWARDS THE ESTABLISHMENT OF FORMULATED DIETS FOR PACIFIC BLUEFIN TUNA (Thunnus orientalis).....................................................................................................................15 Amal Biswas, Biswajit K. Biswas and Kenji Takii BROODSTOCK MONITORING, HANDLING AND INDUCTION TECHNIQUES – RECENT ADVANCES IN ATLANTIC BLUEFIN TUNA (Thunnus thynnus) REPRODUCTION .........20 Chris Bridges, F. Borutta, O. Krohn and S. Schulz RECENT DEVELOPMENTS IN LARVAL AND JUVENILE REARING OF ATLANTIC BLUEFIN TUNA Thunnus thynnus .............................................................................................................25 Massimo Caggiano, M. Campana, M. Moscato, A. Corriero, M. Delflorio, G. Grilli, A. Intini, M.A. Valenza and G. De Metrio STRATEGIES TO CONTROL REPRODUCTION IN SOUTHERN BLUEFIN TUNA (Thunnus maccoyii) IN SOUTH AUSTRALIA...........................................................................................31 Abigail Elizur, M. Diechmann, M. Wise, Y. Zohar, J. Nocillado, Y.Y. Lee, E. Abraham, C. Bridges, C. Mylonas, J. Biran, B. Sivan, W. Knibb, R. Stokoe, E. Bubner, P. Brooks, D. Sullivan, G. Yoshizaki, Y. Takeuchi, P. Thomas Chen and C. Foster ACHIEVEMENTS AND BOTTLENECKS FOR YELLOWFIN TUNA, Thunnus albacares PROPOGATION AT THE GONDOL RESEARCH INSTITUTE FOR MARICULTURE, BALI, INDONESIA ....................................................................................................................34 John Harianto Hutapea, I.G.N. Permana and I.N.A. Giri SOUTHERN BLUEFIN TUNA (Thunnus maccoyii) LARVAL REARING ADVANCES AT THE SOUTH AUSTRALIAN RESEARCH AND DEVELOPMENT INSTITUTE AND COLLABORATING INSTITUTIONS .......................................................................................38 Wayne Hutchinson RESEARCH ON THE REPRODUCTIVE BIOLOGY AND REARING OF LARVAE AND JUVENILES OF YELLOWFIN TUNA (Thunnus albacares) AT THE IATTC’s ACHOTINES LABORATORY, REPUBLIC OF PANAMA.............................................................................43 Dan Margulies, V.P. Scholey, J.B. Wexler and M.C. Santiago THE EFFECT OF A 24-HOUR PHOTOPERIOD ON THE GROWTH AND SURVIVAL OF PRE- FLEXION YELLOWFIN TUNA (Thunnus albacares) LARVAE.............................................46 Gavin Partridge, D. Benetti, J. Hutapea, A. McIntyre, B. Chen, W. Hutchinson, V. Scholey and D. Margulies YELLOWFIN TUNA (Thunnus albacares) AS A CANDIDATE FOR AQUACULTURE IN WESTERN AUSTRALIA...........................................................................................................47 Gavin Partridge and Greg Jenkins TUNA SYMPOSIUM PROCEEDINGS 4 COMPARISON OF LIPID AND FATTY ACID COMPOSITIONS IN DIFFERENT FLESH CUTS OF FARMED FED, FARMED FAST AND WILD PACIFIC BLUEFIN TUNA (Thunnus orientalis).....................................................................................................................................50 Bimol Chandra Roy, Masashi Ando, Ken-ichi Kawasaki and Yasuyuki Tsukamasa ENHANCEMENT OF SURVIVAL RATE OF PACIFIC BLUEFIN TUNA (Thunnus orientalis) LARVAE BY FLOW CONTROL IN REARING TANKS.........................................................55 Wataru Sakamoto and Yoshizumi Nakagawa A WORLD FIRST FOR TUNA: FORMULATED FEED SUCCESS FOR SOUTHERN BLUEFIN TUNA, Thunnus maccoyii ...........................................................................................................59 Richard Smullen TREND OF BLUEFIN TUNA CATCH, REGULATION AND THE PRICE IN THE JAPANESE MARKET ....................................................................................................................................62 Minoru Tada and Sachiko Harada POSTER PRESENTATIONS .............................................................................................................66 USE OF DIFFERENT TYPES OF SOYBEAN MEAL AS ALTERNATIVE PROTEIN SOURCES FOR JUVENILE PACIFIC BLUEFIN TUNA, Thunnus orientalis............................................66 Biswajit K. Biswas, Sho Naito, Amal Biswas, Yang-Su Kim, Kenjii Takii THE CHALLENGE OF DOMESTICATION OF BLUEFIN TUNA Thunnus thynnus – HIGHLIGHTS OF THE SELFDOTT PROJECT FROM 2008-2009 ..................................................................70 F. de al Gandara, C.C. Mylonas, D. Coves, C.R. Bridges, A. Belmonte Rios, R. Vassallo- Agius, H. Rosenfeld, A. Medina, G. Demetrio, J. Falcon, K. Sveinsvoll, A. Ghysen, S. Deguara THE ATLANTIC BLUEFIN TUNA (Thunnus thynnus) SPAWNING IN CAPTIVITY......................73 de la Gandara, C.C. Mylonas, D. Coves, C.R. Bridges, A. Belmonte Rios, R. Vassallo- Agius, H. Rosenfeld, A. Medina, G. Demetrio, J. Falcon, K. Sveinsvoll, A. Ghysen, S. Deguara BIOSECURITY PROTOCOLS FOR TRANSLOCATION OF SOUTHERN BLUEFIN TUNA (Thunnus macoyii) EGGS FROM SOUTH AUSTRALIA TO NEW SOUTH WALES, PORT STEPHENS FISHERIES INSTITUTE........................................................................................79 Stewart Fielder, Geoff Allan, Jeffrey Go, Mike Thomson and Paul Hardy-Smith SKRETTING’S COMMITMENT TO BLUEFIN TUNA......................................................................81 Rhys Hauler, Gavin Shaw and Angus Denwood AGE AND GROWTH OF FATTENING BLUEFIN TUNA (Thunnus thynnus L., 1758) IN THE EASTERN MEDITERRANEAN SEA........................................................................................82 N. Milatour and P. Megalofonou INFECTION AND PARASITIC NEMATODES IN AUSTRALIAN MARINE FISH: PUBLIC HEALTH IMPORTANCE ..........................................................................................................85 Shokoofeh Shamsi and Andreas Lopata HATCHING SUCCESS AND EARLY LARVAL DEVELOPMENT OF SOUTHERN BLUEFIN TUNA (Thunnus maccoyii) .........................................................................................................88 L.D. Woolley, J.G. Qin, M. Thomson and A. Czypionka AUTHOR INDEX.................................................................................................................................93 TUNA SYMPOSIUM PROCEEDINGS 5 PREFACE It is my immense pleasure to welcome all participants to the 2nd Global COE Program Symposium of Kinki University “Sustainable Aquaculture of the Bluefin and Yellowfin Tuna—Closing the Life Cycle for Commercial Production”. The recent problems with avian influenza have encouraged people to increase their consumption of fish to reduce health risks. Moreover, the high meat quality and great taste of bluefin tuna make this species among the most important fish in the world. Consequently, the demand for this species has increased sharply during the last decades. To cope with the increasing demand, a cage culture industry, based on the collection of juveniles from the wild, has developed in different countries. However, this industry increases the risk of resource depletion and adds pressure to the fragile ecosystem on which tuna depend. Therefore, the world is now very eager to develop technologies for increasing bluefin tuna resources and produce fingerlings for culture purposes. Kinki University achieved a full culture cycle of the Pacific bluefin tuna for the first time in the world in 2002. In addition, research collaboration between Kinki University and Clean Seas Tuna Limited has seen the successful completion of fingerling production of Southern bluefin tuna for the first time in the world. It is believed that the tuna industry will benefit from these golden achievements with the Pacific and Southern bluefin tuna. This Symposium will cover talks on different aspects of Pacific and Southern bluefin tuna as well as yellowfin tuna. We hope that this symposium will meet all your expectations and provide you with the opportunity to learn about the latest advances in bluefin and yellowfin tuna aquaculture. Apart from the Global COE Program of Kinki University, we believe that this symposium would not have been possible without the enthusiastic support from Clean Seas Tuna Limited, South Australian Research and Development Institute (SARDI), Australian Seafood Cooperative Research Center, Industry and Investment NSW, and the Australian Fisheries Research and Development Cooperation. We thank all these sponsors as well as the Japanese and Australian organizing committees for their tireless and excellent contribution and support to make this symposium a great success. We also thank the editors of the Symposium Proceedings for their efforts