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United States Patent (10) Patent No.: US 9,572,363 B2 Langan Et Al

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United States Patent (10) Patent No.: US 9,572,363 B2 Langan Et Al USOO9572363B2 (12) United States Patent (10) Patent No.: US 9,572,363 B2 Langan et al. (45) Date of Patent: *Feb. 21, 2017 (54) METHODS FOR THE PRODUCTION AND 6,569.475 B2 5/2003 Song USE OF MYCELIAL LIQUID TISSUE 9,068,171 B2 6/2015 Kelly et al. CULTURE 2002/0082418 A1* 6/2002 Ikewaki ................... A61K 8,73 536,123.12 2002.01371.55 A1 9, 2002 Wasser et al. (71) Applicant: Mycotechnology, Inc., Aurora, CO 2003/0208796 A1* 11/2003 Song ......................... A23L 1.28 (US) 800,297 2004.0009143 A1 1/2004 Golz-Berner et al. (72) Inventors: James Patrick Langan, Denver, CO 29:59: A. 658. E.tametS et al. (US);S. RHuntington eyes Davis, Broomfield, o 2005/02551262005/0180989 A1 11/20058/2005 TSubakiMatsunaga et al. CO (US) 2005/0273875 A1 12, 2005 Elias 2006, OO14267 A1 1/2006 Cleaver et al. (73) Assignee: MYCOTECHNOLOGY, INC., Aurora, 2006/0134294 A1* 6/2006 McKee .................... A23G 1/32 CO (US) 426,548 2006/0280753 A1 12/2006 McNeary - r 2007/O160726 A1 7/2007 Fujii (*) Notice: Subject to any disclaimer, the term of this 2008/0031892 A1 2/2008 Kristiansen patent is extended or adjusted under 35 2008/0057162 A1 3/2008 Brucker et al. U.S.C. 154(b) by 0 days. 2008/O107783 A1 5/2008 Anijs et al. This patent is Subject to a terminal dis- (Continued) claimer. FOREIGN PATENT DOCUMENTS (21) Appl. No.: 14/836,830 CN 102860541. A 1, 2013 DE 4341316 6, 1995 (22) Filed: Aug. 26, 2015 (Continued) (65) Prior Publication Data OTHER PUBLICATIONS US 2016/0058049 A1 Mar. 3, 2016 Beuchat "Indigenous Fermented Foods', in Biotechnology Set, Second Edition (eds H.-J. Rehm and G. Reed), Wiley-VCH Verlag Related U.S. Application Data GmbH. Weinheim, Germany, p. 505-559, 2001.* AV Nowrousian et al. “The novel ER membrane protein PRO41 is (60) Provisional application No. 62/042,071, filed on Aug. essential for sexual development in the filamentous fungus Sordaria 26, 2014. macrospora' Molecular microbiology 64(4): 923-937, 2007.* JP S6291161A machine translated by EPO website.* (51) Int. Cl. Office Action issued Feb. 19, 2014 for U.S. Appl. No. 13/844,685. A2.3L I/28 (2006.01) Autoclave Search (2014) www.meriam-Webster.com/medlineplus/ autoclave. A2.3L. I./22 (2006.01) Berovic et al. (2003) “Submerged cultivation of Ganoderma A2.3L I/236 (2006.01) luciucidum biomass and immunostimulatory effects of fungal polysac A2.3L I/23 (2006.01) charides' J. Biotechnol. 103(1): 77-86. (52) U.S. Cl. (Continued) CPC ............ A2.3L I/22083 (2013.01); A23K 10/12 (2016.05); A23K 10/16 (2016.05); A23K 10/30 Primary Examiner — Emily Cordas (2016.05); A23K 20/163 (2016.05); A23L (74) Attorney, Agent, or Firm — Swanson & Bratschun, 27/24 (2016.08); A23L 27/33 (2016.08); A23L L.L.C. 27/86 (2016.08); A23L 27/88 (2016.08); A23L 31/00 (2016.08); A23 V 2002/00 (2013.01) (57) ABSTRACT (58) Field of Classification Search The present invention includes a method for enhancing the S taste of a food product, which includes the steps of culturing ee application file for complete search history. a submerged mycelial- - liquid- - - - tissue- - culture in a media, (56) References Cited collecting the Supernatant fluid of the Submerged mycelial liquid tissue culture, and adding the collected Supernatant U.S. PATENT DOCUMENTS fluid to a food product in an amount Sufficient to enhance the food products taste. In one embodiment, the mycelial liquid 1,822.227 A 9, 1931 Lendrich et al. tissue culture comprises C. Sinensis, and the culture step is 39: A $84. RChan et al carried out for between about one and sixty days. The food 3810997 A 5, 1974 Chien products include Stevia plant parts, Steviol glycoside, aspar 4,590,160 A 5, 1986 Nishihashi et al. tame, acesulfame-K. Sucralose, carbohydrates, monk fruit, 4,891,220 A 1/1990 Donzis cacao, cacao liquor, tea, ginseng, pea protein, Sugar alcohol, 5,934,012 A 1999 Holtz et coffee, cranberry, grapefruit, pomegranate, coconut, wine, 95. f 2.99 E. et al. beer, liquor and spirits. The present invention also includes 6,476.003 B1 1/2002 Jordan et al. products made by the disclosed methods. 6,490,824 B1 12/2002 Intabon et al. 6,558,943 B1 5, 2003 Li et al. 18 Claims, No Drawings US 9,572,363 B2 Page 2 (56) References Cited NL 7322 C 4, 1921 WO WO O1/32830 5, 2001 U.S. PATENT DOCUMENTS WO WO 2006,107208 10, 2006 WO WO 2007/031186 3, 2007 2008/0171104 A1 7, 2008 Zhu WO WO 2011/O1268O 2, 2011 2008/0193595 A1 8/2008 De Vuyst et al. WO WO 2011/032244 3, 2011 2008/0264858 A1 10, 2008 Stamets WO WO 2011, 151831 12/2011 2008/0274234 A1 11, 2008 Miller WO WO 2013, 171194 11 2013 2008/0296,223 A1 12, 2008 Hiromoto WO WO 2014f145256 A1 9, 2014 2008/0299645 A1 12/2008 Holliday WO WO 2014f145265 A2 9, 2014 2009/0047236 A1 2/2009 Stamets WO PCT/US16, 19929 2, 2016 2009.0053363 A1 2/2009 An WO WO 2016/033241 3, 2016 2009/0098244 A1 4/2009 Schatzmayr et al. WO WO 2016, 138476 9, 2016 2009, O104310 A1 4/2009 Nakajima 2009,013 0138 A1 5, 2009 Stamets OTHER PUBLICATIONS 2009, 0220645 A1 9, 2009 Martinez 2009,0280212 A1 11/2009 Sugimoto et al. Bok et al. Phytochemistry (1999) “Antitumor sterols from the 2010.0055241 A1 3/2010 Nakano et al. mycelia of Cordyceps sinensis' 51: 891-898. 2010, 0183765 A1 7, 2010 Laan Van Der et al. “Can You Eat Mycelium?” (2014) wiki.answers.com. 2010/0203189 A1 8/2010 Holliday de Melo, Rodrigues et al. (2008) Influence of Flammulina velutipes 2010/0203194 A1 8/2010 Salminen et al. mycelia culture conditions on antimicrobial metabolite production 2010, 0221385 A1 9, 2010 Matsui et al. Mycoscience 50(1): 78-81. 2010/0239711 A1 9, 2010 Li "Eat Mycelium cakes?” (2014) Mycotopia.net. 2010/0266726 A1 10/2010 Ogura et al. "Eat Mycelium?” (2014) fungifun.com. 2010/0316763 A1 12/2010 Choi et al. "Eating mycelium to trip” (2014) shroomery.org. 2011 0008384 A1 1/2011 Stamets "Eating Mycelium?” (2014) Zoklet.net. 2011/OO52758 A1 3/2011 Greiner-Stoeffele "Eating Mycelium” (2014) wisegeek.com Conjecture Corporation. 2011 0070332 A1 3/2011 Bernaert et al. Encyclopedia Britannica, Louis Pasteur, Datasheet online. Copy 2011/0081448 A1 4/2011 Dunphy et al. right 2014 Encyclopedia Britannica Inc. retrieved on Feb. 6, 2014). 2011/009 1579 A1 4/2011 Hausman Retrieved from the Internet: <URL: http://www.britannica.com/ 2011/O123675 A1 5, 2011 Bernaert et al. Ebchecked/topic/445964/Louis-Pasteurs. Specif. p. 3. 2011 O189220 A1 8/2011 Yang 2011 (0200551 A1 8, 2011 Stamets English translation.deOliverir, B. etal. WO 2011/032244A1. Flours 2011/0206721 A1 8, 2011 Nair produced from fungus myceliated grain. Published Mar. 24, 2011. 2011/02296.16 A1 9/2011 Anijs et al. pp. 1-24. Specif. pp. 2, 6-7. 2011/0262593 Al 10/2011 Binggeli et al. Firenzuoli et al. (2008) “The Medicinal Mushroom Agaricus blazei 2011/0268980 A1 11 2011 Kalisz et al. Murrill: Review of Literature and Pharmaco-Toxicological Prob 2012fOO27889 A1 2/2012 Portella lems' Evid. Based Complement Alternat. Med. 5(1): 3-15. 2012.0034339 A1 2/2012 Giuliani et al. Foster (2014) “What is Mycelium?' wisegeek.com. Conjecture 2012.0034344 A1 2/2012 Menon et al. Corporation. 2012,0082754 A1 4/2012 Holliday Han (2005) Solid-state fermentation of cornmeal with the basidi 2012/010O249 A1 4/2012 Laan et al. omycete Ganoderma lucidum for degrading starch and upgrading 2012/O128823 A1 5, 2012 Camu 2012/0171308 A1 7, 2012 Da Luz Moreira et al. nutritional value J. Appl. Micro. 2005, 99: 910-915. 2012/0177781 A1 7/2012 Hayashi Hashim, Puziah (1997) “Effect of Processing on Flavour Precursors, 2012/O190093 A1* 7/2012 Fukuda .................... C12N 1.14 Pyrazines and Flavour Quality of Malaysian Cocoa Beans”. PhD 435,165 thesis, Universiti Pertanian Malaysia. 2012/O231114 A1 9, 2012 Bezerra De Oliveira et al. Ikrawan, Yusep (2003) Influence of Carboxypeptidases on Cocoa 2012fO244254 A1 9, 2012 Takahashi Specific Aroma Precursors and Methylpyrazines in Under-Fer 2012/0321744 A1 12/2012 Chhun et al. mented Cocoa Beans. PhD thesis, Universiti Putra Malaysia. 2013/0209608 A1 8, 2013 Berends et al. Ishikawa et al. (2001) "Antimicrobial Cuparene-Type 2013/0209609 A1 8, 2013 Moreno et al. Sesquiterpenes, Enokipodins C and D, from a Mycelial Culture of 2013/03371 14 Al 12/2013 Binggeli et al. Flammulina velutipes' J. Nat. Prod. 64(7): 932-934. 2014/O105928 A1 4/2014 Stamets Kang (2005) Abstract of “Studies on chemical constituents in the 2014/0170264 A1 6/2014 Kelly et al. mycelia from fermented culture of Flammulina velutipes' Zhong 2014/0302560 A1 10/2014 Kelly guo Zhong Yao Za Zhi 30(30): 193-195. 2015,0257405 A1 9/2015 Kelly et al. Kang (2003) Abstract of “Studies on chemical constituents of the 2015,0257406 A1 9/2015 Kelly et al. mycelia from fermented culture of Flammulina velutipes' Zhong 2015/0272155 A1 10/2015 Kelly et al. guo Zhong Yao Za Zhi 28(11): 1038-1040. 2016, 0120201 A9 5/2016 Kelly et al. Konno et al. (2002) “Anticancer and Hypoglycemic Effects of 2016,0249660 A1 9/2016 Langan et al. Polysaccharides in edible and Medicinal Maitake Mushroom Grifola frondosa(Dicks.:Fr.) S. F. Gray” International Journal of FOREIGN PATENT DOCUMENTS Medicinal Mushrooms 4(3): 10-21. Kuo et al.
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