USOO9572363B2 (12) United States Patent (10) Patent No.: US 9,572,363 B2 Langan et al. (45) Date of Patent: *Feb. 21, 2017 (54) METHODS FOR THE PRODUCTION AND 6,569.475 B2 5/2003 Song USE OF MYCELIAL LIQUID TISSUE 9,068,171 B2 6/2015 Kelly et al. CULTURE 2002/0082418 A1* 6/2002 Ikewaki ................... A61K 8,73 536,123.12 2002.01371.55 A1 9, 2002 Wasser et al. (71) Applicant: Mycotechnology, Inc., Aurora, CO 2003/0208796 A1* 11/2003 Song ......................... A23L 1.28 (US) 800,297 2004.0009143 A1 1/2004 Golz-Berner et al. (72) Inventors: James Patrick Langan, Denver, CO 29:59: A. 658. E.tametS et al. (US);S. RHuntington eyes Davis, Broomfield, o 2005/02551262005/0180989 A1 11/20058/2005 TSubakiMatsunaga et al. CO (US) 2005/0273875 A1 12, 2005 Elias 2006, OO14267 A1 1/2006 Cleaver et al. (73) Assignee: MYCOTECHNOLOGY, INC., Aurora, 2006/0134294 A1* 6/2006 McKee .................... A23G 1/32 CO (US) 426,548 2006/0280753 A1 12/2006 McNeary - r 2007/O160726 A1 7/2007 Fujii (*) Notice: Subject to any disclaimer, the term of this 2008/0031892 A1 2/2008 Kristiansen patent is extended or adjusted under 35 2008/0057162 A1 3/2008 Brucker et al. U.S.C. 154(b) by 0 days. 2008/O107783 A1 5/2008 Anijs et al. This patent is Subject to a terminal dis- (Continued) claimer. FOREIGN PATENT DOCUMENTS (21) Appl. No.: 14/836,830 CN 102860541. A 1, 2013 DE 4341316 6, 1995 (22) Filed: Aug. 26, 2015 (Continued) (65) Prior Publication Data OTHER PUBLICATIONS US 2016/0058049 A1 Mar. 3, 2016 Beuchat "Indigenous Fermented Foods', in Biotechnology Set, Second Edition (eds H.-J. Rehm and G. Reed), Wiley-VCH Verlag Related U.S. Application Data GmbH. Weinheim, Germany, p. 505-559, 2001.* AV Nowrousian et al. “The novel ER membrane protein PRO41 is (60) Provisional application No. 62/042,071, filed on Aug. essential for sexual development in the filamentous fungus Sordaria 26, 2014. macrospora' Molecular microbiology 64(4): 923-937, 2007.* JP S6291161A machine translated by EPO website.* (51) Int. Cl. Office Action issued Feb. 19, 2014 for U.S. Appl. No. 13/844,685. A2.3L I/28 (2006.01) Autoclave Search (2014) www.meriam-Webster.com/medlineplus/ autoclave. A2.3L. I./22 (2006.01) Berovic et al. (2003) “Submerged cultivation of Ganoderma A2.3L I/236 (2006.01) luciucidum biomass and immunostimulatory effects of fungal polysac A2.3L I/23 (2006.01) charides' J. Biotechnol. 103(1): 77-86. (52) U.S. Cl. (Continued) CPC ............ A2.3L I/22083 (2013.01); A23K 10/12 (2016.05); A23K 10/16 (2016.05); A23K 10/30 Primary Examiner — Emily Cordas (2016.05); A23K 20/163 (2016.05); A23L (74) Attorney, Agent, or Firm — Swanson & Bratschun, 27/24 (2016.08); A23L 27/33 (2016.08); A23L L.L.C. 27/86 (2016.08); A23L 27/88 (2016.08); A23L 31/00 (2016.08); A23 V 2002/00 (2013.01) (57) ABSTRACT (58) Field of Classification Search The present invention includes a method for enhancing the S taste of a food product, which includes the steps of culturing ee application file for complete search history. a submerged mycelial- - liquid- - - - tissue- - culture in a media, (56) References Cited collecting the Supernatant fluid of the Submerged mycelial liquid tissue culture, and adding the collected Supernatant U.S. PATENT DOCUMENTS fluid to a food product in an amount Sufficient to enhance the food products taste. In one embodiment, the mycelial liquid 1,822.227 A 9, 1931 Lendrich et al. tissue culture comprises C. 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