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(12) United States Patent (10) Patent No.: US 9,057,109 B2 Chang (45) Date of Patent: Jun US009057109B2 (12) United States Patent (10) Patent No.: US 9,057,109 B2 Chang (45) Date of Patent: Jun. 16, 2015 (54) DIAGNOSIS OF MELANOMA AND SOLAR 5,989,815 A 11/1999 Skolnicket al. LENTIGO BY NUCLEC ACID ANALYSIS 6,054,277 A 4/2000 Furcht et al. 6,056,859 A 5/2000 Ramsey et al. 6,129,983 A 10/2000 Schumann et al. (71) Applicant: DermTech International, La Jolla, CA 6,203,987 B1 3/2001 Friend et al. (US) 6,312.909 B1 1 1/2001 Shyjan 6,355.439 B1 3/2002 Chung et al. (72) Inventor: Sherman H. Chang, San Diego, CA 6.410,019 B1 6/2002 DeSimone et al. 6,410,240 B1 6/2002 Hodge et al. (US) 6,551,799 B2 4/2003 Gurney et al. 6,720,145 B2 4/2004 Rheins et al. (73) Assignee: DERMTECH INTERNATIONAL, La 6,726,971 B1 4/2004 Wong Jolla, CA (US) 6,891,022 B1 5/2005 Steward et al. 6,949,338 B2 9, 2005 Rheins et al. 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(2002). CPC ......................................................... C12Q 1/68 (Continued) USPC ........................................................... 435/6.1 See application file for complete search history. Primary Examiner — Ardin Marschel (56) References Cited (74) Attorney, Agent, or Firm — Wilson Sonsini Goodrich & Rosati U.S. PATENT DOCUMENTS 4,365,409 A 12/1982 Riley et al. 4,458,066 A 7, 1984 Caruthers et al. (57) ABSTRACT 4,537,776 A 8/1985 Cooper 4,683, 195 A 7, 1987 Mullis et al. The present invention provides methods for diagnosing mela 4,683.202 A 7, 1987 Mullis et al. noma and/or Solar lentigo in a Subject by analyzing nucleic 4,836,217 A 6, 1989 Fischer et al. 4,851,510 A 7, 1989 Khan acid molecules obtained from the subject. The present inven 4,971,800 A 11, 1990 Chess et al. tion also provides methods for distinguishing melanoma from 5,460,939 A 10/1995 Hansborough et al. 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