DOCSLIB.ORG

Bacillus Pycnus Spa Nov. and Bacillus Neidei Spa Nov., Round-Spored

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Bacillus Pycnus Spa Nov. and Bacillus Neidei Spa Nov., Round-Spored 674 International Journal ofSystematic and Evolutionary Microbiology (2002),52,501-505 DOl: 10.1099/ijs.0.01836-0 Bacillus pycnus Spa nov. and Bacillus neidei Spa NOTE nov., round-spored bacteria from soil 1 Microbial Properties L. K. Nakamura,1 O. Shida/ H. Takagi2 and K. Komagata3 Research Unit, National Center for Agricultural Utilization Research, 1815 N. University Street, Peoria, Author for correspondence: L K. Nakamura. Tel: + 13096816395. Fax: + 13096816672. IL 61604, USA e-mail: nakamulki"mail.ncaur.usda.gov 2 Research Laboratory, Higeta Shoyu Co. Ltd, Bacillus sphaericus sensu lato currently consists of seven or more groups of Choshi, Chiba 288, Japan unrelated taxa, one of which is B. sphaericus sensu stricto and another of 3 Department of which is Bacillus fusiformis. Members of two groups (groups 6 and 7), in Agricultural Chemistry, Tokyo University of common with all other B. sphaericus-like organisms, are unable to grow Agriculture, Setagaya-ku, anaerobically or to use common hexoses, pentoses and hexitols as sources of Tokyo 156, Japan carbon, have G+C contents of 34-36 mol % and form round spores. Groups 6 and 7 can be differentiated from other B. sphaericus-like organisms by low DNA relatedness and by variations in whole-cell fatty acid composition. Unique characteristics of group 6 include the ability to oxidize fi-hydroxybutyrate, the non-requirement for biotin and thiamin and failure to grow in 5 % NaCI. Distinctive traits of group 7 include the inability to oxidize pyruvate and a requirement for biotin, thiamin and cystine for growth. These data show that groups 6 and 7 represent two novel species, for which the names Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., respectively, are proposed; the corresponding type strains are NRRL NRS-1691 T (= JCM 11075T) and NRRL BD-87T (= JCM 11077T). Keywords: Bacillus PYC/lUS, Bacillus /leidei, novel species, 165 rDNA The round-spored, mesophilic species Bacillus sphaeri­ comprised B. fils(jonnis: group I appeared to be a cus was first described by Neide (1904). Because early subgroup of 2. Groups 4-7 were unknown taxa. differentiation tests \vere ineffective, all round-spored In the present study, DNA relatedness evaluation and mesophiles were classified as B. sphaericus: the lack of phenotypic characterization were used to differentiate adequate differentiating tools deterred taxonomic groups 6 and 7 from each other, from representative study of mesophilic, round-spored bacteria. However, strains of groups 4 and 5 and from type strains of the discovery ofmosquitocidal activity among some of recognized round-spare-forming relatives. these organisms (Kellen et al., 1965) and the de­ velopment of molecular-biological techniques have DNA was extracted from 24-h cultures from groups I rekindled taxonomic and phylogenetic examination of (NRRL B-23269), 4 (NRRL NRS-593), 5 (NRRL T the round-spored organisms. Taxonomic heterogen­ B-1876), 6 (NRRL NRS-1691 , NRRL NRS-1693, eity of B. sphaericus had been suggested by variability NRRL NRS-1694, NRRL NRS-1695) and 7 (NRRL T of insecticidal activity among strains and was con­ BD-87 , NRRL BD-IOL NRRL BD-I03) and the firmed by comparative studies using DNA similarity following type cultures: 'Bacillus aminovorans' DSM T assessment (Krych et al., 1980), numerical taxonomy 4337, B. fils(jormis DSM 2898 , Sporosarcina globis­ T (Alexander & Priest, 1990: Priest et al., 1988), ran­ pora NRRL NRS-1533 , Bacillus insolitus NRRL T domly amplified polymorphic DNA fingerprinting NRS-1531 T, Marinibacillusmarinus DSM 1297 ,Sporo­ (Woodburn et al., 1995) and rRNA gene restriction sarcina psychrophila IFO 15381 T, B. silvestris DSM T T (Aquino de Muro et al., 1992). These developments led 12223 , B. sphaericus JCM 2502 and Ureibacillus T to the discovery of two more mesophilic species, thermosphaericus DSM 10633 . DNA reassociation Bacillus fils(lormis (Priest et al., 1988) and Bacillus was carried out using the method ofEzaki et al. (1989). silvestris (Rheims et al., 1999). In a phylogenetic study Probes were prepared with DNA from B. sphaericus T T based on 16S rDNA sequences (Nakamura, 2000), B. JCM 2502 , B. tllsijormis DSM 2898 , NRRL NRS­ T T sphaericus-like species segregated into seven groups 1691 • NRRL NRS-1694. NRRL BD-87 and NRRL wherein group 3 represented B. sphaericus and group 2 BD-I03. 01836 Printed in Great Britain 501 L. K. Nakamura and others Group 1 !-=::::::::::==~-I Group 2 93 Bacillus fusiformis Group 3 99 B. sphaericus Group 4 65 95 L-----I Group 5 '--------- B. silvestris NRS·1695 (AF169535) ] NRS·1691 T (AF169531) Group 6 ,..-__--"""00'-1 NRS·1694 (AF169534) (B. pycnussp. nov.) NRS·1693 (AF169533) 77 '-----i100 80-101 (AF169508) ] 82 Group 7 '-----1110<00 80-103 (AF169509) (B. neidei sp. nov.) 80-87 (AF169520) 89 L...... _ B. thermosphaericus P·11 (X90640) ........----1'00 B. psychrophilus ATCC 23304 T (X60634) B. globisporus OSM 4 T (X68415) ,..----1'00 L..... B. pasteurii NCIMB 8841 (X60631) L..... B. insolitus OSM 5 T (X60642) ........------ 'B. aminovorans'NCIMB 8292 (X62178) '-------- B. marinus OSM 1297 T (AJ237708) B. atrophaeus NCIMB 12899 (X60607) ,...------j1GG 99 B. subtilis NCOO 1769 (X60646) B. licheniformis OSM 13 T (X68416) '-------- B. cereus NCTC 11143 (X55063) ,...------ B.lentus lAM 12466T (016272) UL------- B. circulans lAM 12462 T (078312) '------ B. megaterium lAM 13418T (016273) 76 ,...------------- B. stearothermophilus T10 (X57309) L- B. smithii OSM 4216T (X60643) L.- ''"'oo''-j B. laevo/acticus lAM 12322 (016269) 7' 'B. myxolacticus'IAM 12326 (016274) -----------r;;;;;--- Brevibacillus centrosporus NRRL NRS·664T (078458) ,... '00 T 72 B. brevis JCM 2503 (078457) T L------------j'oo Aneurinibacillus migulanus ATCC 9999 (078462) A. aneurinilyticus NCIMB 10056 (X60645) -------G~========'00 Paenibacillus larvae NCDO 1141 (X60036) ~ P. polymyxa NCOO 1774 (60632) L..... Alicyclobacillus cycloheptanicus 1457 (X51928) Fig_ 1. Neighbour-joining tree showing the phylogenetic position of groups 6 and 7 among the mesophilic and psychrophilic round-spored Bacillus species and selected oval-spored species. The tree is based on 1321-nt sequences. Confidence limits estimated from bootstrap analyses (500 replicates) appear at the nodes. This is a modification of the tree generated by Nakamura (2000). A maximum-parsimony tree generated from the sequence data showed a similar topology. Alicyclobacillus cycloheptanicus was designated the outgroup species for the analysis. B. thermosphaericus has been reclassified as Ureibacillus thermosphaericus (Fortina et al., 2001); B. globisporus, B. psychrophilus and B. pasteurii have been reclassified as Sporosarcina globispora, Sporosarcina psychrophila and Sporosarcina pasteurii, respectively (Yoon et al., 2001 a); B. marinus has been reclassified as Marinibacillus marinus (Yoon et al., 2001 b); and B. stearothermophilus has been reclassified as Geobacillus stearothermophilus (Nazina et al., 2001). Bar, 1 nt substitution per 10 nt. Substrate oxidation profiles were obtained using the mercially prepared antibiotic discs on TGY agar plates BIOLOG GP system under conditions prescribed by spread-inoculated with the test organisms. The test the manufacturer. Other physiological traits were plates were incubated at 28 DC and the extent of examined using the methods of Gordon et al. (1973). inhibition was determined at 48 h. Decomposition Antibiotic sensitivity was assessed by placing com- of Tweens 40 and 80 was determined by the method 502 International Journal ofSystematic and Evolutionary Microbiology 52 Bacillus PYCllllS sp. nov. and B. neidei sp. nov. of Breuil & Gounot (1972). Vitamin and amino acid Table 1. Traits useful for differentiating the requirements were determined using the method of B. sphaericus-like groups Proom el al. (1955). ~ The BIOLOG system was used to evaluate substrate The extent of similarity among strains based on fatty oxidation. Substrates listed are those givin a 0 or 100 % acid composition data (Nakamura, 2000) was esti­ reactions. L-Glutamate was used bv all stn~ins: 64 substrates mated using the simple matching coefficient and were not used at all and variable r~actions were obtained with 26 substrates. AII organisms tested were sensitive to clustering was based on the unweighted pair group er~thromvcin. method with arithmetic averages (Sneath & SokaL chloramphenicol, tetracvcline and tobramvcin. Groups: 1 and 2 (n ~ 20), B..fils(fbrll1i~·: 3 (n = 7), . 1973). All cultures were grown on trypticase soy agar B..ljJ!IaericlIs: 4 (n = 10), unknown: 5 (n = 7). unknown: for 24 hat 30°C. Cell-wall peptidoglycan composition 6 (n = 3). B. pycnlls sp. nov.: and 7 (n = 4), B. neidei sp. nov. was analysed using the method described by Suzuki el al. (1993). Character Group In a phylogenetic tree based on 16S rDNA sequences, strains of groups 4-7 were positioned within a clade I and 2 3 4 5 6 7 that consisted generally of mesophilic, round-spored species, namely B. sphaericlls, B. jils(jorillis and Substrate oxidized: B. silveslris; the thermophile, U. lherillosphaericlls, is Pyruvate T + + + + situated outside the clade [Fig. 1; this tree is a fJ-Hydroxybutyrate + modification of one generated by Nakamura (2000)]. L-Alanine + + + + High bootstrap values validate the uniqueness of Glycyl L-glutamate + + + + + groups 4-7 within the clade of round-spored organ­ 2'-Deoxyadenosine + + + + + isms. Groups 6 and 7 were more closely related to Inosine + + + + each other than to the other mesophilic groups. AlvIP + + + UMP + + + High levels of
Load more

Recommended publications
  • The Role of Earthworm Gut-Associated Microorganisms in the Fate of Prions in Soil
    THE ROLE OF EARTHWORM GUT-ASSOCIATED MICROORGANISMS IN THE FATE OF PRIONS IN SOIL Von der Fakultät für Lebenswissenschaften der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Taras Jur’evič Nechitaylo aus Krasnodar, Russland 2 Acknowledgement I would like to thank Prof. Dr. Kenneth N. Timmis for his guidance in the work and help. I thank Peter N. Golyshin for patience and strong support on this way. Many thanks to my other colleagues, which also taught me and made the life in the lab and studies easy: Manuel Ferrer, Alex Neef, Angelika Arnscheidt, Olga Golyshina, Tanja Chernikova, Christoph Gertler, Agnes Waliczek, Britta Scheithauer, Julia Sabirova, Oleg Kotsurbenko, and other wonderful labmates. I am also grateful to Michail Yakimov and Vitor Martins dos Santos for useful discussions and suggestions. I am very obliged to my family: my parents and my brother, my parents on low and of course to my wife, which made all of their best to support me. 3 Summary.....................................................………………………………………………... 5 1. Introduction...........................................................................................................……... 7 Prion diseases: early hypotheses...………...………………..........…......…......……….. 7 The basics of the prion concept………………………………………………….……... 8 Putative prion dissemination pathways………………………………………….……... 10 Earthworms: a putative factor of the dissemination of TSE infectivity in soil?.………. 11 Objectives of the study…………………………………………………………………. 16 2. Materials and Methods.............................…......................................................……….. 17 2.1 Sampling and general experimental design..................................................………. 17 2.2 Fluorescence in situ Hybridization (FISH)………..……………………….………. 18 2.2.1 FISH with soil, intestine, and casts samples…………………………….……... 18 Isolation of cells from environmental samples…………………………….……….
    [Show full text]
  • Biomineralization Mediated by Ureolytic Bacteria Applied to Water Treatment: a Review
    crystals Review Biomineralization Mediated by Ureolytic Bacteria Applied to Water Treatment: A Review Dayana Arias 1,2 ID , Luis A. Cisternas 2,3 ID and Mariella Rivas 1,3,* 1 Laboratory of Algal Biotechnology & Sustainability, Faculty of Marine Sciences and Biological Resources, University of Antofagasta, Antofagasta 1240000, Chile; [email protected] 2 Department of Chemical Engineering and Mineral Process, University of Antofagasta, Antofagasta 1240000, Chile; [email protected] 3 Science and Technology Research Center for Mining CICITEM, Antofagasta 1240000, Chile * Correspondence: [email protected] Academic Editor: Jolanta Prywer Received: 6 October 2017; Accepted: 4 November 2017; Published: 17 November 2017 Abstract: The formation of minerals such as calcite and struvite through the hydrolysis of urea catalyzed by ureolytic bacteria is a simple and easy way to control mechanisms, which has been extensively explored with promising applications in various areas such as the improvement of cement and sandy materials. This review presents the detailed mechanism of the biominerals production by ureolytic bacteria and its applications to the wastewater, groundwater and seawater treatment. In addition, an interesting application is the use of these ureolytic bacteria in the removal of heavy metals and rare earths from groundwater, the removal of calcium and recovery of phosphate from wastewater, and its potential use as a tool for partial biodesalination of seawater and saline aquifers. Finally, we discuss the benefits of using biomineralization processes in water treatment as well as the challenges to be solved in order to reach a successful commercialization of this technology. Keywords: biomineralization; calcite; seawater; wastewater; heavy metals removal; biodesalination 1.
    [Show full text]
  • Genome Diversity of Spore-Forming Firmicutes MICHAEL Y
    Genome Diversity of Spore-Forming Firmicutes MICHAEL Y. GALPERIN National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894 ABSTRACT Formation of heat-resistant endospores is a specific Vibrio subtilis (and also Vibrio bacillus), Ferdinand Cohn property of the members of the phylum Firmicutes (low-G+C assigned it to the genus Bacillus and family Bacillaceae, Gram-positive bacteria). It is found in representatives of four specifically noting the existence of heat-sensitive vegeta- different classes of Firmicutes, Bacilli, Clostridia, Erysipelotrichia, tive cells and heat-resistant endospores (see reference 1). and Negativicutes, which all encode similar sets of core sporulation fi proteins. Each of these classes also includes non-spore-forming Soon after that, Robert Koch identi ed Bacillus anthracis organisms that sometimes belong to the same genus or even as the causative agent of anthrax in cattle and the species as their spore-forming relatives. This chapter reviews the endospores as a means of the propagation of this orga- diversity of the members of phylum Firmicutes, its current taxon- nism among its hosts. In subsequent studies, the ability to omy, and the status of genome-sequencing projects for various form endospores, the specific purple staining by crystal subgroups within the phylum. It also discusses the evolution of the violet-iodine (Gram-positive staining, reflecting the pres- Firmicutes from their apparently spore-forming common ancestor ence of a thick peptidoglycan layer and the absence of and the independent loss of sporulation genes in several different lineages (staphylococci, streptococci, listeria, lactobacilli, an outer membrane), and the relatively low (typically ruminococci) in the course of their adaptation to the saprophytic less than 50%) molar fraction of guanine and cytosine lifestyle in a nutrient-rich environment.
    [Show full text]
  • 12092016 Ni Xiang Phd Dissertation.Pdf
    Biological Control Potential of Spore-forming Plant Growth-Promoting Rhizobacteria Suppressing Meloidogyne incognita on Cotton and Heterodera glycines on Soybean by Ni Xiang A dissertation submitted to the Graduate Faculty of Auburn University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy Auburn, Alabama December 10, 2016 Keywords: biological control, Plant Growth-Promoting Rhizobacteria (PGPR), Meloidogyne incognita, cotton, Heterodera glycines, soybean Copyright 2016 by Ni Xiang Approved by Kathy S. Lawrence, Chair, Professor of Entomology and Plant Pathology Joseph W. Kloepper, Professor of Entomology and Plant Pathology Edward J. Sikora, Professor of Entomology and Plant Pathology David B. Weaver, Professor of Crop, Soil, and Environmental Sciences Dennis P. Delaney, Extension Specialist of Crop, Soil, and Environmental Sciences Abstract The objective of this study was to screen a library of PGPR strains to determine activity to plant-parasitic nematodes with the ultimate goal of identifying new PGPR strains that could be developed into biological nematicide products. Initially a rapid assay was needed to distinguish between live and dead second stage juveniles (J2) of H. glycines and M. incognita. Once the assay was developed, PGPR strains were evaluated in vitro and selected for further evaluation in greenhouse, microplot, and field conditions. Three sodium solutions, sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3), and sodium hydroxide (NaOH) were evaluated to distinguish between viable live and dead H. glycines and M. incognita J2. The sodium solutions applied to the live J2 stimulated the J2 to twist their bodies in a curling shape and increased movement activity. Optimum movement of H. glycines was observed with the application of 1 µl of Na2CO3 (pH =10) added to the 100 µl suspension.
    [Show full text]
  • Characterization of Ureolytic Bacteria Isolated from Limestone Caves of Sarawak and Evaluation of Their Efficiency in Biocementation
    CHARACTERIZATION OF UREOLYTIC BACTERIA ISOLATED FROM LIMESTONE CAVES OF SARAWAK AND EVALUATION OF THEIR EFFICIENCY IN BIOCEMENTATION By ARMSTRONG IGHODALO OMOREGIE A thesis presented in fulfilment of the requirements for the degree of Master of Science (Research) Faculty of Engineering, Computing and Science SWINBURNE UNIVERSITY OF TECHNOLOGY 2016 ABSTRACT The aim of this study was to isolate, identify and characterise bacteria that are capable of producing urease enzyme, from limestone cave samples of Sarawak. Little is known about the diversity of bacteria inhabiting Sarawak’s limestone caves with the ability of hydrolyzing urea substrate through urease for microbially induced calcite precipitation (MICP) applications. Several studies have reported that the majority of ureolytic bacterial species involved in calcite precipitation are pathogenic. However, only a few non-pathogenic urease-producing bacteria have high urease activities, essential in MICP treatment for improvement of soil’s shear strength and stiffness. Enrichment culture technique was used in this study to target highly active urease- producing bacteria from limestone cave samples of Sarawak collected from Fairy and Wind Caves Nature Reserves. These isolates were subsequently subjected to an increased urea concentration for survival ability in conditions containing high urea substrates. Urea agar base media was used to screen for positive urease producers among the bacterial isolates. All the ureolytic bacteria were identified with the use of phenotypic and molecular characterizations. For determination of their respective urease activities, conductivity method was used and the highly active ureolytic bacteria isolated comparable with control strain used in this study were selected and used for the next subsequent experiments in this study.
    [Show full text]
  • Improving the Strength of Sandy Soils Via Ureolytic Caco3 Solidification by Sporosarcina Ureae
    Improving the Strength of Sandy Soils via Ureolytic CaCO3 Solidification by Sporosarcina ureae Justin Michael Whitaker1, Sai Vanapalli2, and Danielle Fortin1; 5 1Department of Earth and Environmental Sciences (413-ARC). University of Ottawa, K1N 6N5, Ottawa, ON, Canada 2Department of Civil Engineering (A015-CBY). University of Ottawa, K1N 6N5, Ottawa, ON, Canada 10 Correspondence to: D. Fortin ([email protected]) Key words: Urease, calcite precipitation, MICP, Sporosarcina, Bacillus, biomineralisation, biofilm Abstract 15 'Microbial induced carbonate precipitation' (MICP) is a biogeochemical process that can be applied to strengthen materials. The hydrolysis of urea by microbial catalysis to form carbonate is a commonly studied example of MICP. In this study, Sporosarcina ureae, a ureolytic organism, was compared to other ureolytic and non-ureolytic organisms of Bacillus and Sporosarcina in the assessment of its ability to produce carbonates by ureolytic MICP for 20 ground reinforcement. It was found that S. ureae grew optimally in alkaline (pH ~9.0) conditions which favoured MICP and could degrade urea (units [U] /mL = µmol/min.mL.OD600) at levels (30.28 U/mL) similar to S. pasteurii (32.76 U/mL), the model ureolytic MICP organism. When cells of S. ureae were concentrated (OD600 ~15-20) and mixed with cementation medium containing 0.5 M calcium chloride (CaCl2) and urea into a model sand, repeated treatments (3 x 24 h) were able to improve the confined direct shear strength of samples from 15.77 kPa to as much as 135.80 kPa. This was more 25 than any other organism observed in the study. Imaging of the reinforced samples with scanning electron microscopy and energy dispersive spectroscopy confirmed the successful precipitation of calcium carbonate (CaCO3), across sand particles by S.
    [Show full text]
  • Characterization and Identification of Some Aerobic Spore- Forming Bacteria Isolated from Saline Habitat, West Coastal Region, Saudi Arabia
    IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN:2278-3008, p-ISSN:2319-7676. Volume 12, Issue 2 Ver. II (Mar. - Apr.2017), PP 14-19 www.iosrjournals.org Characterization and Identification of Some Aerobic Spore- Forming Bacteria Isolated From Saline Habitat, West Coastal Region, Saudi Arabia 1* 1 2 1,3 Naheda Alshammari , Fatma Fahmy , Sahira Lari and Magda Aly 1Biology Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, 2Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia, 3Botany Department, Faculty of Science, Kafr el-Sheikh University, Egypt Abstract: Ten isolates of aerobic endospore- forming moderately halophilic bacteria were isolated fromsaline habitat at the west coastal region near Jeddah. All isolates that were Gram positive, catalase positive andshowing different colony morphology and shapes were studied. They were mesophilic, neutralophilic, with temperature range 20-40°C and pH range 7-9. The isolates were separating into two distinct groups facultative anaerobic strictly aerobic. One isolate was identified as Paenibacillus dendritiformis, two isolates as Bacillus oleronius, two isolates as P. alvei, three isolates belong to B. subtilis and B. atrophaeus, and finally two isolates was identified as Bacillus sp. Furthermore, two aerobic endospore-forming cocci, isolated from salt-march soil in Germany were tested for their taxonomical status and used as reference isolates and these isolates belong to the species Halobacillus halophilus. Chemotaxonomic characteristics represented by cell wall analysis and fatty acid profiles of some selected isolates were studied to determine the differences between species. Keywords: Halobacillus, Bacillus, spore, mesophilic, physiological, morphological I. Introduction Aerobic spore-forming bacteria represent a major microflora in many natural biotopes and play an important role in ecosystem development.
    [Show full text]
  • Bacteria Species NCBI Sequence ID Bacteria Species NCBI
    Bacteria Species NCBI Sequence ID Bacteria Species NCBI Sequence ID Achromobacter deleyi HG324053.1 Gordonia amarae AF020329.1 Achromobacter kerstersii HG324052.1 AF020330.1 Achromobacter pestifer HG324051.1 AF020331.1 Achromobacter spiritinus HE613447.1 AF020332.1 Acidithiobacillus ferrooxidans AM890075.2 NR_037032.1 AH001793.2 Halomonas denitrificans AM229317.1 Acinetobacter baumannii AB594765.1 Halomonas elongata AM941743.1 Acinetobacter calcoaceticus AB626122.1 Halomonas janggokensis AM229315.1 Acinetobacter lwoffii AB626125.1 Halomonas sp. BH1 FN433898.1 Acinetobacter sp. Z93454.1 Halomonas sp. SR4 JF794994.1 FM177774.1 Janthinobacterium agaricidamnosum AB681849.1 Agrobacterium radiobacter AJ389904.1 Janthinobacterium lividum AB021388.1 AJ130719.1 Janthinobacterium sp. AB252072.1 AM181758.1 AJ846273.1 Aminobacter aminovorans LT984904.1 AM071372.1 LN995690.1 Kocuria carniphila AJ622907.1 Aminobacter anthyllidis FR869633.2 Kocuria flava HQ331530.1 Aminobacter sp. AB905480.1 Kocuria polaris AJ278868.1 FM886907.1 Kocuria sp. LT674162.1 Arthrobacter oxydans LN880075.1 AB773222.1 LN774480.1 Labrys methylaminiphilus AB236172.1 Arthrobacter sp. KM362724.1 Labrys miyagiensis AB236170.1 Azospirillum brasilense AB681745.1 AB236171.1 DQ438999.1 Labrys okinawensis AB236169.1 Azospirillum lipoferum LN874293.1 Labrys sp. LC372609.1 Azospirillum oryzae AB185396.1 Lysinibacillus composti AB547124.1 Azospirillum zeae HE977584.1 Lysinibacillus pakistanensis AB558495.1 Azotobacter chroococcum AB430880.1 Lysinibacillus parviboronicapiens AB681953.1 LN874290.1 Lysinibacillus sp. HG931343.1 Azotobacter vinelandii LK391702.1 Lysinibacillus sphaericus JX485781.1 LN874283.1 Mycobacterium colombiense AM062764.1 LN874283.1 Mycobacterium haemophilum L24800.1 Bacillus caldolyticus EF472596.1 Mycobacterium phlei JX266706.1 Bacillus caldotenax EU484354.1 KF378762.1 AY952967.1 NR_041906.1 Bacillus cereus NR_074540.1 GU142920.1 Bacillus clarkii X76444.1 GU142927.1 Bacillus mucilaginosus AY571332.1 Mycobacterium ratisbonense AJ271863.1 DQ898310.1 Mycobacterium sp.
    [Show full text]
  • Can Microbially Induced Calcite Precipitation (MICP) Through a Ureolytic Pathway Be Successfully Applied for Removing Heavy Metals from Wastewaters?
    crystals Review Can Microbially Induced Calcite Precipitation (MICP) through a Ureolytic Pathway Be Successfully Applied for Removing Heavy Metals from Wastewaters? Álvaro Esteban Torres-Aravena 1,2,* , Carla Duarte-Nass 2, Laura Azócar 3, Rodrigo Mella-Herrera 2, Mariella Rivas 4,5 and David Jeison 6 1 Departamento de Ingeniería Química, Universidad de La Frontera, Temuco 4780000, Chile 2 Núcleo Científico y Tecnológico de Biorecursos (BIOREN), Universidad de La Frontera, Temuco 4780000, Chile; [email protected] (C.D.-N.); [email protected] (R.M.-H.) 3 Departamento de Química Ambiental, Facultad de Ciencias, Universidad Católica de la Santísima Concepción, Concepción 4090541, Chile; [email protected] 4 Centro de Investigación Científico Tecnológico para la Minería CICITEM, Antofagasta 1240000, Chile; [email protected] 5 Laboratorio de Biotecnología Algal y Sustentabilidad, Facultad de Ciencias del Mar y recursos Biológicos, Universidad de Antofagasta, Antofagasta 1240000, Chile 6 Escuela de Ingeniería Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, Valparaíso 2362803, Chile; [email protected] * Correspondence: [email protected] Received: 11 October 2018; Accepted: 2 November 2018; Published: 21 November 2018 Abstract: Microbially induced calcite precipitation (MICP) through a ureolytic pathway is a process that promotes calcite precipitation as a result of the urease enzymatic activity of several microorganisms. It has been studied for different technological applications, such as soil bio-consolidation, bio-cementation, CO2 sequestration, among others. Recently, this process has been proposed as a possible process for removing heavy metals from contaminated soils. However, no research has been reported dealing with the MICP process for heavy metal removal from wastewater/waters.
    [Show full text]
  • B. Cereus, Généralités
    Adaptation au froid de la bactérie pathogène Bacillus cereus : étude de mécanismes impliqués et exploitation de la diversité génétique Sara Esther Diomande To cite this version: Sara Esther Diomande. Adaptation au froid de la bactérie pathogène Bacillus cereus : étude de mécanismes impliqués et exploitation de la diversité génétique. Agronomie. Université d’Avignon, 2014. Français. NNT : 2014AVIG0666. tel-01408068 HAL Id: tel-01408068 https://tel.archives-ouvertes.fr/tel-01408068 Submitted on 3 Dec 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Ecole Doctorale Agrosciences et Sciences Thèse Pour obtenir le titre de Docteur de l’Université d’Avignon et des Pays de Vaucluse Discipline : Biotechnologie et Microbiologie Sara Esther DIOMANDE Adaptation au froid de la bactérie pathogène Bacillus cereus : étude de mécanismes impliqués et exploitation de la diversité génétique Soutenue publiquement le 02 Décembre 2014 devant le Jury composé de : Rapporteurs Dr. Emmanuelle BOUVERET Directrice de recherche, CNRS, Marseille Dr. Pascale SERROR Directrice de Recherche, INRA Jouy-en-Josas Examinateurs Pr. Catherine DUPORT Professeur de l’Université d’Avignon Pr. Marc FEUILLOLEY Professeur de l’Université de Rouen Dr. Vincent SANCHIS Directeur de recherche, INRA Jouy-en Josas Directeurs de thèse Dr.
    [Show full text]
  • Yoon Et Al (2001) Sporosarcina Aquimarina Sp.Nov.Pdf
    International Journal of Systematic and Evolutionary Microbiology (2001), 51, 1079–1086 Printed in Great Britain Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina 1 Korea Research Institute of Jung-Hoon Yoon,1 Keun-Chul Lee,1 Norbert Weiss,4 Yung Hee Kho,1 Bioscience and 3 1,2 Biotechnology (KRIBB), Kook Hee Kang and Yong-Ha Park PO Box 115, Yusong, Taejon, Korea Author for correspondence: Yong-Ha Park. Tel: 82 42 860 4620. Fax: 82 42 860 4598. 2 j j Probionic Corporation e-mail: yhpark!mail.kribb.re.kr Bio-venture Centre, Korea Research Institute of Bioscience and Biotechnology (KRIBB), A light-orange-coloured, facultatively anaerobic, rod-shaped bacterium (strain PO Box 115, Yusong, SW28T), which was isolated from seawater in Korea, was taxonomically studied Taejon, Korea by a polyphasic approach. This organism formed round terminal endospores in 3 Department of Food and swollen sporangia. The peptidoglycan type is A4α, based on L-Lys-L-Ala-D-Asp. Life Science, The predominant menaquinone is MK-7 and the major fatty acid is ante-C15:0. Sungkyunkwan University, The GMC content of the DNA is 40 mol%. Phylogenetic analysis based on 16S Chunchun-dong 300, rDNA sequences showed that strain SW28T falls within the radiation of a Jangan-gu, Suwon, Korea cluster comprising the rRNA group 2 bacilli and non-Bacillus-type organisms.
    [Show full text]
  • Metagenomics and Metatranscriptomics of Lake Erie Ice
    METAGENOMICS AND METATRANSCRIPTOMICS OF LAKE ERIE ICE Opeoluwa F. Iwaloye A Thesis Submitted to the Graduate College of Bowling Green State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE August 2021 Committee: Scott Rogers, Advisor Paul Morris Vipaporn Phuntumart © 2021 Opeoluwa Iwaloye All Rights Reserved iii ABSTRACT Scott Rogers, Lake Erie is one of the five Laurentian Great Lakes, that includes three basins. The central basin is the largest, with a mean volume of 305 km2, covering an area of 16,138 km2. The ice used for this research was collected from the central basin in the winter of 2010. DNA and RNA were extracted from this ice. cDNA was synthesized from the extracted RNA, followed by the ligation of EcoRI (NotI) adapters onto the ends of the nucleic acids. These were subjected to fractionation, and the resulting nucleic acids were amplified by PCR with EcoRI (NotI) primers. The resulting amplified nucleic acids were subject to PCR amplification using 454 primers, and then were sequenced. The sequences were analyzed using BLAST, and taxonomic affiliations were determined. Information about the taxonomic affiliations, important metabolic capabilities, habitat, and special functions were compiled. With a watershed of 78,000 km2, Lake Erie is used for agricultural, forest, recreational, transportation, and industrial purposes. Among the five great lakes, it has the largest input from human activities, has a long history of eutrophication, and serves as a water source for millions of people. These anthropogenic activities have significant influences on the biological community. Multiple studies have found diverse microbial communities in Lake Erie water and sediments, including large numbers of species from the Verrucomicrobia, Proteobacteria, Bacteroidetes, and Cyanobacteria, as well as a diverse set of eukaryotic taxa.
    [Show full text]