PRB4 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science PRB4 Rabbit pAb Catalog No.: A16701 Basic Information Background Catalog No. This gene encodes a member of the heterogeneous family of basic, proline-rich, human A16701 salivary glycoproteins. The encoded preproprotein undergoes proteolytic processing to generate one or more mature peptides before secretion from the parotid glands. Observed MW Multiple alleles of this gene exhibiting variations in the length of the tandem repeats 31kDa have been identified. The reference genome encodes the "Small" allele. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 12. Calculated MW Alternative splicing results in multiple transcript variants encoding different isoforms 31kDa that may undergo similar proteolytic processing. Category Primary antibody Applications WB Cross-Reactivity Human, Mouse Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 5545 P10163 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 178-247 of human PRB4 (NP_002714.2). Synonyms PRB4;Po Contact Product Information 400-999-6126 Source Isotype Purification Rabbit IgG Affinity purification [email protected] www.abclonal.com.cn Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using PRB4 antibody (A16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Cow's Milk Allergy in Dutch Children
Petrus et al. Clin Transl Allergy (2016) 6:16 DOI 10.1186/s13601-016-0105-z Clinical and Translational Allergy RESEARCH Open Access Cow’s milk allergy in Dutch children: an epigenetic pilot survey Nicole C. M. Petrus1*†, Peter Henneman2†, Andrea Venema2, Adri Mul2, Femke van Sinderen2, Martin Haagmans2, Olaf Mook2, Raoul C. Hennekam1,2, Aline B. Sprikkelman1‡ and Marcel Mannens2‡ Abstract Background: Cow’s milk allergy (CMA) is a common disease in infancy. Early environmental factors are likely to con- tribute to CMA. It is known that epigenetic gene regulation can be altered by environmental factors. We have set up a proof of concept study, aiming to detect epigenetic associations specific with CMA. Methods: We studied children from the Dutch EuroPrevall birth cohort study (N 20 CMA, N 23 controls, N 10 tolerant boys), age and gender matched. CMA was challenge proven. Bisulfite converted= DNA =(blood) was analyzed= using the 450K infinium DNA-methylation array. Four groups (combined, girls, boys and tolerant boys) were analysed between CMA and controls. Statistical analysis and pathway-analysis were performed in “R” using IMA, Minfi and the global-test package. Differentially methylated regions in DHX58, ZNF281, EIF42A and HTRA2 genes were validated by quantitative amplicon sequencing (ROCHE 454®). Results: General hypermethylation was found in the CMA group compared to control children, while this effect was absent in the tolerant group. Methylation differences were, among others, found in regions of DHX58, ZNF281, EIF42A and HTRA2 genes. Several of these genes are known to be involved in immunological pathways and associated with other allergies. Conclusion: We show that epigenetic associations are involved in CMA.
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UI Health Care Letterhead Template
An international effort towards developing standards for best practices in analysis, interpretation and reporting of clinical genome sequencing results in the CLARITY Challenge The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Citation Brownstein, Catherine A. et al. "An international effort towards developing standards for best practices in analysis, interpretation and reporting of clinical genome sequencing results in the CLARITY Challenge." Genome Biology 15:.3 (2014). p.1-18. As Published http://dx.doi.org/10.1186/gb-2014-15-3-r53 Publisher BioMed Central Ltd. Version Final published version Citable link http://hdl.handle.net/1721.1/88017 Terms of Use Creative Commons Attribution Detailed Terms http://creativecommons.org/licenses/by/2.0 University of Iowa Hospitals and Clinics Department of Otolaryngology – Head & Neck Surgery 200 Hawkins Drive, 21151-PFP Iowa City, IA 52242-1078 319-356-3612 Tel 319-356-4108 Fax www.uihealthcare.com September 24, 2012 Katherine Flannery Program Manager Harvard Medical School Center for Biomedical Informatics Email: [email protected] RE: CLARITY Challenge Dear Katherine, Accompanying this letter is our CLARITY Challenge reports. As stipulated in the protocol and instructions, our reports are not focused on identifying unrelated findings associated with other health issues or diseases. Pursuant to these specific aims and objectives, we have completed the following: 1. Analysis of whole genome and whole exome sequence data on three families; 2. Identification of potential alterations associated with the phenotype in the proband; 3. Determination and identification of key components for reporting these results; 4.
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