Nitric Oxide/Cgmp Pathway Signaling Actively Down-Regulates Α4β1-Integrin Affinity: an Unexpected Mechanism for Inducing Cell De- Adhesion Chigaev Et Al
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Bacillus Anthracis Edema Factor Substrate Specificity: Evidence for New Modes of Action
Toxins 2012, 4, 505-535; doi:10.3390/toxins4070505 OPEN ACCESS toxins ISSN 2072–6651 www.mdpi.com/journal/toxins Review Bacillus anthracis Edema Factor Substrate Specificity: Evidence for New Modes of Action Martin Göttle 1,*, Stefan Dove 2 and Roland Seifert 3 1 Department of Neurology, Emory University School of Medicine, 6302 Woodruff Memorial Research Building, 101 Woodruff Circle, Atlanta, GA 30322, USA 2 Department of Medicinal/Pharmaceutical Chemistry II, University of Regensburg, D-93040 Regensburg, Germany; E-Mail: [email protected] 3 Institute of Pharmacology, Medical School of Hannover, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany; E-Mail: [email protected] * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +1-404-727-1678; Fax: +1-404-727-3157. Received: 23 April 2012; in revised form: 15 June 2012 / Accepted: 27 June 2012 / Published: 6 July 2012 Abstract: Since the isolation of Bacillus anthracis exotoxins in the 1960s, the detrimental activity of edema factor (EF) was considered as adenylyl cyclase activity only. Yet the catalytic site of EF was recently shown to accomplish cyclization of cytidine 5′-triphosphate, uridine 5′-triphosphate and inosine 5′-triphosphate, in addition to adenosine 5′-triphosphate. This review discusses the broad EF substrate specificity and possible implications of intracellular accumulation of cyclic cytidine 3′:5′-monophosphate, cyclic uridine 3′:5′-monophosphate and cyclic inosine 3′:5′-monophosphate on cellular functions vital for host defense. In particular, cAMP-independent mechanisms of action of EF on host cell signaling via protein kinase A, protein kinase G, phosphodiesterases and CNG channels are discussed. -
Supporting Table S3 for PDF Maker
Supplemental Table S3. Annotation of identified proteins. Number of Sequence Accession Number of Theoretical Subcellular Number Locus ID Gene Name Protein Name Identified Coverage Theoretical pI Protein Family NSAF Number Amino Acid MW (Da) Location of TMD Peptides (%) (O08539) Myc box-dependent-interacting protein 1 O08539 BIN1_MOUSE BIN1 (Bridging integrator 1) (Amphiphysin-like protein) 3 10.5 588 64470 5 Nucleus other NONE 5.73E-05 (Amphiphysin II) (SH3-domain-containing protein 9) (O08547) Vesicle-trafficking protein SEC22b (SEC22 O08547 SC22B_MOUSE SEC22B 5 30.4 214 24609 8.5 Cytoplasm other 2 0.000262 vesicle-trafficking protein-like 1) (O08553) Dihydropyrimidinase-related protein 2 (DRP-2) O08553 DPYL2_MOUSE DPYSL2 5 19.4 572 62278 6.4 Cytoplasm enzyme NONE 7.85E-05 (ULIP 2 protein) O08579 EMD_MOUSE EMD (O08579) Emerin 1 5.8 259 29436 5 Nucleus other 1 8.67E-05 (O08583) THO complex subunit 4 (Tho4) (RNA and transcription O08583 THOC4_MOUSE THOC4 export factor-binding protein 1) (REF1-I) (Ally of AML-1 1 9.8 254 26809 11.2 Nucleus NONE 2.21E-05 regulator and LEF-1) (Aly/REF) O08585 CLCA_MOUSE CLTA (O08585) Clathrin light chain A (Lca) 2 4.7 235 25557 4.5 Plasma Membrane other NONE 0.000287 (O08600) Endonuclease G, mitochondrial precursor (EC O08600 NUCG_MOUSE ENDOG 4 23.1 294 32191 9.5 Cytoplasm enzyme NONE 0.000134 3.1.30.-) (Endo G) (O08638) Myosin-11 (Myosin heavy chain, smooth O08638 MYH11_MOUSE MYH11 8 6.6 1972 227026 5.5 Cytoplasm other NONE 3.13E-05 muscle isoform) (SMMHC) (O08648) Mitogen-activated protein kinase kinase -
Dependent Protein Kinase to Cyclic CMP Agarose
Binding of Regulatory Subunits of Cyclic AMP- Dependent Protein Kinase to Cyclic CMP Agarose Andreas Hammerschmidt1., Bijon Chatterji2., Johannes Zeiser2, Anke Schro¨ der2, Hans- Gottfried Genieser3, Andreas Pich2, Volkhard Kaever1, Frank Schwede3, Sabine Wolter1", Roland Seifert1*" 1 Institute of Pharmacology, Hannover Medical School, Hannover, Germany, 2 Institute of Toxicology, Hannover Medical School, Hannover, Germany, 3 Biolog Life Science Institute, Bremen, Germany Abstract The bacterial adenylyl cyclase toxins CyaA from Bordetella pertussis and edema factor from Bacillus anthracis as well as soluble guanylyl cyclase a1b1 synthesize the cyclic pyrimidine nucleotide cCMP. These data raise the question to which effector proteins cCMP binds. Recently, we reported that cCMP activates the regulatory subunits RIa and RIIa of cAMP- dependent protein kinase. In this study, we used two cCMP agarose matrices as novel tools in combination with immunoblotting and mass spectrometry to identify cCMP-binding proteins. In agreement with our functional data, RIa and RIIa were identified as cCMP-binding proteins. These data corroborate the notion that cAMP-dependent protein kinase may serve as a cCMP target. Citation: Hammerschmidt A, Chatterji B, Zeiser J, Schro¨der A, Genieser H-G, et al. (2012) Binding of Regulatory Subunits of Cyclic AMP-Dependent Protein Kinase to Cyclic CMP Agarose. PLoS ONE 7(7): e39848. doi:10.1371/journal.pone.0039848 Editor: Andreas Hofmann, Griffith University, Australia Received May 11, 2012; Accepted May 31, 2012; Published July 9, 2012 Copyright: ß 2012 Hammerschmidt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. -
Mullergliaregnerationtranscriptome
gene.id fc1 fc2 fc3 fc4 p1 p2 p3 p4 FDR.pvalue-1 Gene Symbol Gene Title Pathway go biological process term go molecular function term go cellular component term Dr.10016.1.A1_at -2.33397 -3.86923 -4.38335 -2.39965 0.935201 0.320614 0.208 0.917227 0.208 zgc:77556 zgc:77556 --- proteolysis arylesterase activity /// metallopeptidase activity /// zinc ion --- binding Dr.10024.1.A1 at 1.483417 2.531269 2.089091 1.698761 1 0.613 0.998961 1 0.613 zgc:171808 zgc:171808--- cell-cell signaling --- --- Dr.10051.1.A1 at -1.78449 -2.22024 -1.70922 -1.99464 1 0.901663 1 0.999955 0.9017 ccng2 cyclin G2 --- --- --- --- Dr.10061.1.A1 at 2.065955 2.274632 2.248958 2.507754 0.992 0.718655 0.83 0.600609 0.6006 zgc:173506 zgc:173506--- --- --- --- Dr.10061.2.A1 at 2.131883 2.616483 2.49378 2.815337 0.983443 0.711513 0.805599 0.519115 0.5191 zgc:173506 Zgc:17350 --- --- --- --- Dr.10065.1.A1 at -1.02315 -2.01596 -2.29343 -1.88944 1 0.999955 0.957199 1 0.9572 zgc:114139 zgc:114139--- --- --- cytoplasm /// centrosome Dr.10070.1.A1_at -1.74365 -2.52206 -2.39093 -1.86817 1 0.741254 0.885401 1 0.7413 fbp1a fructose-1, --- carbohydrate metabolic process hydrolase activity /// phosphoric ester hydrolase activity --- Dr.10074.1.S1_at 6.035545 10.44051 7.880519 5.020371 0.1104 0.044 0.062491 0.144679 0.044 pdgfaa platelet-der--- multicellular organismal development /// cell proliferation growth factor activity membrane Dr.10095.1.A1 at -1.73408 -2.11615 -1.47234 -2.19919 1 0.997562 1 0.978177 0.9782 wu:fk95g04 wu:fk95g04--- --- --- --- Dr.10110.1.S1 a at 3.929761 5.798708 -
Discovery of Novel Functional Centers with Rationally Designed Amino Acid Motifs Aloysius Wong, Xuechen Tian, Chris Gehring, Claudius Marondedze
Discovery of Novel Functional Centers With Rationally Designed Amino Acid Motifs Aloysius Wong, Xuechen Tian, Chris Gehring, Claudius Marondedze To cite this version: Aloysius Wong, Xuechen Tian, Chris Gehring, Claudius Marondedze. Discovery of Novel Functional Centers With Rationally Designed Amino Acid Motifs. Computational and Structural Biotechnology Journal, Elsevier, 2018, 16, pp.70 - 76. 10.1016/j.csbj.2018.02.007. hal-01851991 HAL Id: hal-01851991 https://hal.archives-ouvertes.fr/hal-01851991 Submitted on 26 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License Computational and Structural Biotechnology Journal 16 (2018) 70–76 Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/csbj Discovery of Novel Functional Centers With Rationally Designed Amino Acid Motifs Aloysius Wong a,⁎, Xuechen Tian a,ChrisGehringb, Claudius Marondedze c a Department of Biology, Wenzhou-Kean University, 88 Daxue Road, Ouhai, Wenzhou, Zhejiang Province -
ED Bobo Orcid.Org 0000-0002-1558-1704
Molecular identification and functional characterization of a novel adenylyl cyclase from Glycine max ED Bobo orcid.org 0000-0002-1558-1704 Thesis accepted in fulfilment of the requirements for the degree Doctor of Philosophy in Biology at the North-West University Promoter: Prof O Ruzvidzo Co-supervisor: Dr SS Mlambo Co-supervisor: Dr TD Kawadza Graduation ceremony: July 2020 Student number: 27537730 PREFACE AND ACKNOWLEDMENTS This research work is a ground-breaking discovery in the study of secondary messengers in plants commonly known as cyclic adenosine monophosphate (cAMP) which are important signalling molecules. cAMP synthesis is made possible through the action of adenylyl cyclase (AC) enzymes that are responsible for increasing their concentration in cells. This ground-breaking research focused on cloning and expression of the first ever AC in Glycine max; XP_003529590, gene ID Glyma.07G251000 against a background of only 8 cloned and expressed ACs in higher plants. Five of these are from Arabidopsis thaliana, the other three from Hippaestrum hybridium, Nicotiana tabacum and Zea mays. The goal of the research was to elucidate the functional roles of the novel AC in soybean through molecular and bioinformatic characterisation. A lot of experimental work was covered in the Plant Biotechnology laboratory at the North West University in Mafikeng, South Africa to make this project a success. First and fore-most I would want to express my sincere gratitude to my mentor and promoter Professor Oziniel Ruzvidzo for trusting and believing in me that I had it in me to handle a project of such great magnitude as I was coming from a purely ecological background. -
On the Neurobiology of Physical Activity in Mice and Human in Search of Eating Disorders Determinants
On the Neurobiology of Physical Activity in Mice and Human In Search of Eating Disorders Determinants Elżbieta Kostrzewa ISBN 9789-0393-6090-3 Printed by: CPI Koninklijke Wöhrmann Layout: Elżbieta Kostrzewa and Wouter Wiltenburg Cover design: Elżbieta Kostrzewa and Wouter Wiltenburg More artwork at surrella.wordpress.com . This cover was inspired by The Elephant in the Room , Banksy exhibition, 2006 Barely Legal show, Los Angeles. © Elżbieta Kostrzewa On the Neurobiology of Physical Activity in Mice and Human In Search of Eating Disorders Determinants Over de Neurobiologie van Lichaamsbeweging in Muizen en Mens Op Zoek naar Determinanten van Eetstoornissen (met een samenvatting in het Nederlands) Badania nad neurobiologicznymi podstawami aktywności fizycznej myszy i ludzi W poszukiwaniu determinantów zaburzeń odżywiania (ze streszczeniem w języku polskim) Proefschrift ter verkrijging van de graad van doctor aan de Universiteit Utrecht op gezag van de rector magnificus, prof.dr. G.J. van der Zwaan, ingevolge het besluit van het college voor promoties in het openbaar te verdedigen op donderdag 6 februari 2014 des ochtends te 10.30 uur door Elżbieta Kostrzewa geboren op 10 maart 1983 te Krakau, Polen Promotor: Prof. dr. R.A.H. Adan Co-promotor: Dr. M.J.H. Kas Table of Contents CHAPTER 1 07 General Introduction Physical Activity 08 Eating Disorders 29 Translational Approach in Genetic Studies 43 Scope of the Thesis 49 CHAPTER 2 53 A Candidate Syntenic Genetic Locus is Associated with Physical Activity Levels in Mice and Humans CHAPTER 3 77 -
An Unusual and Physiologically Vital Protein with Guanylate Cyclase and P-Type Atpase Like Domain in a Pathogenic Protist
bioRxiv preprint doi: https://doi.org/10.1101/475848; this version posted November 23, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. Cyclic GMP signaling in Toxoplasma gondii An unusual and physiologically vital protein with guanylate cyclase and P-type ATPase like domain in a pathogenic protist Özlem Günay-Esiyok1, Ulrike Scheib2, Matthias Noll1, Nishith Gupta1* 1 Institute of Biology, Department of Molecular Parasitology, Faculty of Life Sciences, Humboldt University, Berlin, Germany 2 Institute of Biology, Department of Experimental Biophysics, Faculty of Life Sciences, Humboldt University, Berlin, Germany Correspondence * Dr. Nishith Gupta [email protected] Running title: Cyclic GMP signaling in Toxoplasma gondii ABSTRACT Cyclic GMP is considered as one of the master regulators of diverse functions in eukaryotes; its architecture and functioning in protozoans remain poorly understood however. We characterized an unusual and extra-large guanylate cyclase (477-kDa) containing at least 4 putative P-type ATPase motifs and 21 transmembrane helices in a common parasitic protist, Toxoplasma gondii. This protein, termed as TgATPaseP-GC due to its anticipated multi-functionality, localizes in the plasma membrane at the apical pole, while the corresponding cGMP-dependent protein kinase (TgPKG) is distributed in cytomembranes. Both proteins are expressed constitutively during the entire lytic cycle of the parasite in human cells, which suggests a post-translational control of cGMP signaling. Homology modeling indicated an activation of guanylate cyclase by heterodimerization of its two cyclase domains. -
And TNP-Nucleotides for Inhibition of Rat Soluble Guanylyl Cyclase Α1β1
Molecular Pharmacology Fast Forward. Published on January 27, 2014 as DOI: 10.1124/mol.113.091017 Molecular PharmacologyThis article hasFast not beenForward. copyedited Published and formatted. on The January final version 27, may 2014 differ as from doi:10.1124/mol.113.091017 this version. MOL #91017 Structure/activity relationships of (M)ANT- and TNP-nucleotides for inhibition of rat soluble guanylyl cyclase α1β1 Stefan Dove, Kerstin Yvonne Danker, Johannes-Peter Stasch, Downloaded from Volkhard Kaever, Roland Seifert molpharm.aspetjournals.org Department of Medicinal Chemistry II, University of Regensburg, Regensburg, Germany (SD) Institute of Pharmacology, Hannover Medical School, Hannover, Germany (KYD, VK, RS) at ASPET Journals on September 24, 2021 Institute of Cardiovascular Research, Bayer HealthCare, Wuppertal, Germany (JPS) Research Core Unit Metabolomics, Hannover Medical School, Hannover, Germany (VK) Primary laboratory of origin: Institute of Pharmacology, Hannover Medical School, Carl- Neuberg-Str. 1, D-30625 Hannover, Germany. 1 Copyright 2014 by the American Society for Pharmacology and Experimental Therapeutics. Molecular Pharmacology Fast Forward. Published on January 27, 2014 as DOI: 10.1124/mol.113.091017 This article has not been copyedited and formatted. The final version may differ from this version. MOL #91017 Running title: Inhibitors of soluble guanylyl cyclase Corresponding author: Dr. Roland Seifert, Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Phone: -
XXI Fungal Genetics Conference Abstracts
Fungal Genetics Reports Volume 48 Article 17 XXI Fungal Genetics Conference Abstracts Fungal Genetics Conference Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Fungal Genetics Conference. (2001) "XXI Fungal Genetics Conference Abstracts," Fungal Genetics Reports: Vol. 48, Article 17. https://doi.org/10.4148/1941-4765.1182 This Supplementary Material is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact [email protected]. XXI Fungal Genetics Conference Abstracts Abstract XXI Fungal Genetics Conference Abstracts This supplementary material is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol48/iss1/17 : XXI Fungal Genetics Conference Abstracts XXI Fungal Genetics Conference Abstracts Plenary sessions Cell Biology (1-87) Population and Evolutionary Biology (88-124) Genomics and Proteomics (125-179) Industrial Biology and Biotechnology (180-214) Host-Parasite Interactions (215-295) Gene Regulation (296-385) Developmental Biology (386-457) Biochemistry and Secondary Metabolism(458-492) Unclassified(493-502) Index to Abstracts Abstracts may be cited as "Fungal Genetics Newsletter 48S:abstract number" Plenary Abstracts COMPARATIVE AND FUNCTIONAL GENOMICS FUNGAL-HOST INTERACTIONS CELL BIOLOGY GENOME STRUCTURE AND MAINTENANCE COMPARATIVE AND FUNCTIONAL GENOMICS Genome reconstruction and gene expression for the rice blast fungus, Magnaporthe grisea. Ralph A. Dean. Fungal Genomics Laboratory, NC State University, Raleigh NC 27695 Rice blast disease, caused by Magnaporthe grisea, is one of the most devastating threats to food security worldwide. -
12) United States Patent (10
US007635572B2 (12) UnitedO States Patent (10) Patent No.: US 7,635,572 B2 Zhou et al. (45) Date of Patent: Dec. 22, 2009 (54) METHODS FOR CONDUCTING ASSAYS FOR 5,506,121 A 4/1996 Skerra et al. ENZYME ACTIVITY ON PROTEIN 5,510,270 A 4/1996 Fodor et al. MICROARRAYS 5,512,492 A 4/1996 Herron et al. 5,516,635 A 5/1996 Ekins et al. (75) Inventors: Fang X. Zhou, New Haven, CT (US); 5,532,128 A 7/1996 Eggers Barry Schweitzer, Cheshire, CT (US) 5,538,897 A 7/1996 Yates, III et al. s s 5,541,070 A 7/1996 Kauvar (73) Assignee: Life Technologies Corporation, .. S.E. al Carlsbad, CA (US) 5,585,069 A 12/1996 Zanzucchi et al. 5,585,639 A 12/1996 Dorsel et al. (*) Notice: Subject to any disclaimer, the term of this 5,593,838 A 1/1997 Zanzucchi et al. patent is extended or adjusted under 35 5,605,662 A 2f1997 Heller et al. U.S.C. 154(b) by 0 days. 5,620,850 A 4/1997 Bamdad et al. 5,624,711 A 4/1997 Sundberg et al. (21) Appl. No.: 10/865,431 5,627,369 A 5/1997 Vestal et al. 5,629,213 A 5/1997 Kornguth et al. (22) Filed: Jun. 9, 2004 (Continued) (65) Prior Publication Data FOREIGN PATENT DOCUMENTS US 2005/O118665 A1 Jun. 2, 2005 EP 596421 10, 1993 EP 0619321 12/1994 (51) Int. Cl. EP O664452 7, 1995 CI2O 1/50 (2006.01) EP O818467 1, 1998 (52) U.S. -
( 12 ) United States Patent
US010363295B2 (12 ) United States Patent ( 10 ) Patent No. : US 10 , 363, 295 B2 Schoor et al. ( 45 ) Date of Patent: Jul. 30 , 2019 ( 54 ) PEPTIDES AND COMBINATION OF ( 58 ) Field of Classification Search PEPTIDES FOR USE IN IMMUNOTHERAPY ??? . .. A61K 39 /00 AGAINST NHL AND OTHER CANCERS USPC . .. .. .. .. 424 / 1 . 41 See application file for complete search history . (71 ) Applicant: Immatics Biotechnologies GmbH , Tuebingen (DE ) (56 ) References Cited ( 72 ) Inventors : Oliver Schoor , Tuebingen ( DE ) ; Andrea Mahr , Tuebingen (DE ); Toni U . S . PATENT DOCUMENTS Weinschenk , Aichwald (DE ) ; Anita 9 ,023 , 803 B2 5 / 2015 Singh et al. Wiebe , Ruebgarten (DE ) ; Jens 9 , 056 , 069 B2 6 / 2015 Singh et al . Fritsche , Dusslingen (DE ) ; Harpreet 9 , 791, 443 B2 10 / 2017 Weinschenk et al. Singh , Muenchen Schwabing (DE ) 9 ,791 , 444 B2 10 / 2017 Weinschenk et al. 2008/ 0039413 A1 2 / 2008 Morris et al. 2011/ 0257890 A1 10 / 2011 Weinschenk et al. ( 73 ) Assignee: IMMATICS BIOTECHNOLOGIES 2012 /0027684 A12 / 2012 Singh et al. GMBH , Tuebingen (DE ) 2013 / 0096016 A1 4 /2013 Weinschenk et al . 2013/ 0164254 A1* 6 / 2013 Pieczykolan .. CO7K 14 /70575 ( * ) Notice : Subject to any disclaimer, the term of this 424 / 85 . 1 patent is extended or adjusted under 35 2013 /0177525 A1 7 /2013 Singh et al . U . S . C . 154 ( b ) by 0 days . FOREIGN PATENT DOCUMENTS ( 21 ) Appl. No .: 16 / 193 , 334 EP 1589100 Al 10 /2005 WO 0157273 A2 8 / 2001 ( 22 ) Filed : Nov . 16 , 2018 WO 2011037827 A2 3 / 2011 WO 2011 / 128448 A 10 / 2011 (65 ) Prior Publication Data WO 2011/ 151403 Al 12 / 2011 WO 2015 / 150327 A1 10 / 2015 US 2019 /0076514 A1 Mar .