DOCSLIB.ORG

Poster Session 2 17:20 - 18:20 Thursday, 24Th June, 2021 Tracks Poster Session

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Poster Session 2 17:20 - 18:20 Thursday, 24Th June, 2021 Tracks Poster Session Poster Session 2 17:20 - 18:20 Thursday, 24th June, 2021 Tracks Poster Session 9 Understanding the internal structure of amyloid superstructures with polarization analysis of two-photon excited fluorescence Maciej Lipok, Patryk Obstarczyk, Manuela Grelich-Mucha, Joanna Olesiak-Bańska Advanced Materials Engineering and Modelling Group, Wrocław University of Science and Technology Abstract Text The goal of our study is to develop label-free method for measuring the organization of amyloid fibrils based on their polarization-resolved two-photon excited autofluorescence. For our purpose, we have modified the model used for measuring fibril organization from polarization resolved one-photon fluorescence microscopy and applied it to successfully determine the internal structural organization of fibrils inside insulin spherulites. Amyloids are ordered protein aggregates and a hallmark of multiple neurodegenerative disorders such as Parkinson’s or Alzheimer’s. Understanding the origin and course of amyloidogenesis is a key issue for creation of effective curative methods. A plethora of information about this process could be gathered from studying the organization of amyloid structures in different conditions.[1] In previous years, it was shown that ordering of amyloids stained with Thioflavin T could be efficiently determined using polarization-resolved fluorescence microscopy. [2] However, a label influences the aggregation process [3] and the data obtained from stained structures may differ from the naturally occurring label-free ones. Moreover, correct determination of fibril ordering in densely packed superstructures like amyloid plaques needs high precision which could hardly be achieved using standard fluorescence microscopes. Our method is overcoming this drawbacks, allowing to determine the local ordering of label-free amyloid fibrils in spherulites, amyloid superstructures, using their intrinsic polarization-resolved two-photon excited autofluorescence. [4] However, a new model was needed to analyse the obtained data. We have adapted the theory used with polarization-resolved fluorescence microscopy to two-photon microscopy and successfully applied it for calculating the arrangement of fibrils and their fluorophores in densely packed amyloid spherulites. The sample preparation along with measurement setup and procedure is described in Supporting Information of our aforementioned paper. [4] To calculate the ordering of a spherulite structure we have successfully implemented theoretical models previously used by our group to calculate organization of DNA strands [5] which is a modification of model used for polarization-resolved fluorescence microscopy. [2] Data analysis was performed in Spyder Python 3 IDE. Fitting the theoretical models into experimental data was done using correlation of various model parameters such as shape, intensity, angle maxima location and checked for maximal R2 coefficient. Functions were based on NumPy and SciPy Python libraries. The model assumes that total intensity of two-photon excited fluorescence is dependent on the probability of emission and two-photon absorption, intertwined with molecular distribution function, which is dependent on three structural parameters: rotation of the fibril in XY sample plane, orientational distribution of the emission dipole with respect to the long fibril axis and emission dipole orientational distribution aberrations due to the molecular rotations in filaments (Fig.1). Thus, polarization resolved two-photon fluorescence from highly ordered spots on sample where the average fibril orientation is known could be used to calculate the orientational distribution of fibrils. Appling this model to insulin spherulites have already shown differences between the calculated emission dipole orientational distribution of label-free and stained aggregates or that increasing the salt concentration narrows the emission dipole moment orientational distribution, which was in agreement with calculations of single fibrils imaged using Atomic Force Microscope. In conclusion, we have successfully modified and implemented the model used for polarization resolved fluorescence microscopy to determine the orientational distribution of autofluorescence in amyloids, measured under two-photon microscopy and showed that it differs depending on salt concentration or presence of a dye. Moreover, the presence of polarization dependent alignment of autofluorescence dipole moments supports the mechanism of amyloid autofluorescence, proposed by Grisanti et al. [6] moving us one step closer to understand the origin of amyloid autofluorescence process. Figure 1. Open cone model of the conical distribution of the emission dipole of the dye (half angle, Ψ) in respect to the long fibril axis (dashed line). Rotation of the fibril in the XY microscope sample plane is described by the Φ angle. Aberrations of Ψ due to the molecular rotations in filaments are described by ΔΨ. References [1] M. G. Iadanza, M. P. Jackson, E. W. Hewitt, N. A. Ranson, and S. E. Radford, “A new era for understanding amyloid structures and disease,” Nat. Rev. Mol. Cell Biol., vol. 19, no. 12, pp. 755–773, 2018 [2] J. Duboisset, P. Ferrand, W. He, X. Wang, H. Rigneault, and S. Brasselet, “Thioflavine-T and Congo Red Reveal the Polymorphism of Insulin Amyloid Fibrils When Probed by Polarization-Resolved Fluorescence Microscopy,” J. Phys. Chem. B, vol. 117, no. 3, pp. 784–788, Jan. 2013 [3] M. I. Sulatsky, A. I. Sulatskaya, O. I. Povarova, I. A. Antifeeva, I. M. Kuznetsova, and K. K. Turoverov, “Effect of the fluorescent probes ThT and ANS on the mature amyloid fibrils,” Prion, vol. 14, no. 1, pp. 67–75, Dec. 2020 [4] P. Obstarczyk, M. Lipok, M. Grelich-Mucha, M. Samoć, and J. Olesiak-Bańska, “Two-Photon Excited Polarization-Dependent Autofluorescence of Amyloids as a Label-Free Method of Fibril Organization Imaging,” J. Phys. Chem. Lett., vol. 12, no. 5, pp. 1432–1437, Feb. 2021 [5] H. Mojzisova, J. Olesiak, M. Zielinski, K. Matczyszyn, D. Chauvat, and J. Zyss, “Polarization-Sensitive Two-Photon Microscopy Study of the Organization of Liquid-Crystalline DNA,” Biophys. J., vol. 97, no. 8, pp. 2348–2357, 2009 [6] L. Grisanti, M. Sapunar, A. Hassanali, and N. Došlić, “Toward Understanding Optical Properties of Amyloids: A Reaction Path and Nonadiabatic Dynamics Study,” J. Am. Chem. Soc., vol. 142, no. 42, pp. 18042– 18049, Oct. 2020 11 PYCALIBRATE: FULLY AUTOMATED PSF ANALYSIS Alexander Corbett University of Exeter Abstract Text STANDARDISING IMAGE ANALYSIS: One of the key barriers to data reproducibility is the lack of standardization in microscope quality control (QC). Microscope QC requires standardization of both the sample used to measure microscope performance and the software used to analyse the images acquired. Despite there being several commercial (e.g. SVI Huygens) and freely available (PSFJ, MetroloJ) software solutions available there is currently no single package that is widely used by the microscopy community. Moreover, the solutions that do exist are semi-automated, requiring user input to define acquisition parameters and providing a window for error. This lack of automation and standardization makes performance comparisons between different microscopes unreliable. FULLY AUTOMATED ANALYSIS: In response to the above problems, PyCalibrate was developed. PyCalibrate is able to provide fully automated analysis of images of point-like objects (e.g. beads or PSFcheck slide features) that describe the microscope point spread function (PSF). Using a “scale-space” approach to the image analysis, the PyCalibrate algorithm is able to automatically determine the feature size of the PSF features in a 3D data set. Once the individual features have been identified, the lateral and axial full width at half maxima (FWHM) values are determined. By applying a 2D Gaussian fit to the XY plane, major and minor widths, as well as orientation of the major axis can be quantified. GLOBALLY ACCESSIBLE HISTORY: To avoid problems associated with platform-dependent performance and maintaining the most recent software version, PyCalibrate has been developed as a web app. This requires only an internet connection to upload raw data to the web app and then download the analysis as a PDF or CSV file. As previous records are maintained in the cloud, this allows the full history of your microscope to be recorded to track sudden changes or slow drifts in performance. Using a cloud-based solution, data can be uploaded, processed and the results retrieved from anywhere in the world. References [1] www.psfcheck.com 14 JIPipe: Designing automated image analysis pipelines without programming Ruman Gerst1,2, Zoltán Cseresnyés1, Marc Thilo Figge1,2 1Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology – Hans-Knöll- Institute, Jena, Germany.. 2Faculty of Biological Sciences, Friedrich-Schiller-University Jena, Germany Abstract Text JIPipe is a powerful visual programming language that provides an intuitive way to create batch processing pipelines in ImageJ1 via an easy-to-use graphical interface that suitable for beginners and advanced users. Figure 1: JIPipe makes the development of image analysis pipelines intuitive and easy to follow. (a) The general workflow to analyze data like such a set of C. elegans time series images with the purpose to extract the maximum covered area per worm. The process involves importing the data, applying thresholding and postprocessing, generating statistics, and finally storing
Load more

Recommended publications
  • Indirik Kuleler(Castella, Praesidia) Veya Birçok Yerlerde De Yalnız Münferit Kuleler (Turres) Inşa Edilmiş Bulunmaktaydı
    6 KÜLTÜR BAKANLI(;IYAYINLARV1l93 ;i; Tırell,.Eli,., DlzJsU81 Dr. KONSTANTİN YOSİF İREÇEK BELGRAD-�TANBUL-ROMA ASKERiYOLU ir H.lt( j( li' ııphonesi �, - Mı.itlürü Bulgarcadan çeviren Ali Kemal BALKANLı Kapak Dliıeni: Nur OKAN ISBN 975 - 17 - 0768 - 4 © Kültür Bakanlı�ı, 1990 - Yayımlar Dairesi Başkanlı!ı'nın 4.1.1990tarih ve TERED 928-5 -1- 1715 sayılı makam onayı ile birinci defa olarak 5.000 adet bastınlmış­ br. Sistem Ofset - ANKARA (Dr. Konstantın Yosif) iREÇEK Çek asıllı Avusturyalıtarih ve coQrafyacl olup 1854 yılında Viyana'da doQmuştur; 1918 yılında yine Viyana'da ölen Ire­ çek, Balkan milletlerinin tarihleri ve Balkanların coQrafi özel­ likleri hakkında incelemeleriyle şÖhret kazanmıştır. Prag Üniversitesi'ni bitirmiş sonra aynı üniversitede Do­ çent olarak hizmet görmüştür. Eserleri 1876'da yazmış oldu­ Qu "Die Geschihte der Bulgaren (Bulgarlar'ın tarihi), bunu müteakip kaleme aldlQı "FGrstentum Bulgarien" (Bulgaristan PrensliQi), "Geschichte Serben" (Sırplar'ın tarihi) ve tercüme­ sini sunduQum VIA MILlTARIS (Roma askeri yolu) SINGIDUNUM - CONSTANlINOPOLlS (Belgrad- Istanbul) dur. 1879'da Bulgar hükümeti tarafından Sofya'ya davet edi­ len Ireçek, 5 yıl çalışarak, Bulgar milli eQitimini yeni bir düze­ ne koymuştur. V ONSOZ Tarihin en eski ve bugünkü insana henüz karanltk çabla­ rmdan beri, daha henüz az sayıda insanlardan oluşan toplu­ luklardan başlayarak �demobullart, bir birleriyle olan mal altşverişlerinde veya savaşlarda hep tabiatm geçişe elverişli kıldıbı yol ve geçit/erden gelip geçerek temasa gelmişlerdir. Özellikle, bütün uluslarm genel bir kaynaşmayla yurtlarmı bı­ rakarak batıya dobru akm ettikleri ortaçablarda, kalabaltk topluluklar halindeki dobu millet/eri geçiş ve akmlartna en uy­ gun bulduklart tabii yollart kullanmışlard". Bazııart Hazardenizi'yle Kara Deniz'i kuzeyden dolaşarak A vrupa'ya geçmiş, bir kısmı da Anadolu yartmadasma geçe­ rek kolay aşılabilen bobazlar yoluyla Avrupa'ya gelip yerleş­ mişler veya Ege adalarmdan basamak basamak sıçrayarak daha ötelere gitmişlerdir.
    [Show full text]
  • Name, a Novel
    NAME, A NOVEL toadex hobogrammathon /ubu editions 2004 Name, A Novel Toadex Hobogrammathon Cover Ilustration: “Psycles”, Excerpts from The Bikeriders, Danny Lyon' book about the Chicago Outlaws motorcycle club. Printed in Aspen 4: The McLuhan Issue. Thefull text can be accessed in UbuWeb’s Aspen archive: ubu.com/aspen. /ubueditions ubu.com Series Editor: Brian Kim Stefans ©2004 /ubueditions NAME, A NOVEL toadex hobogrammathon /ubueditions 2004 name, a novel toadex hobogrammathon ade Foreskin stepped off the plank. The smell of turbid waters struck him, as though fro afar, and he thought of Spain, medallions, and cork. How long had it been, sussing reader, since J he had been in Spain with all those corkoid Spanish medallions, granted him by Generalissimo Hieronimo Susstro? Thirty, thirty-three years? Or maybe eighty-seven? Anyhow, as he slipped a whip clap down, he thought he might greet REVERSE BLOOD NUT 1, if only he could clear a wasp. And the plank was homely. After greeting a flock of fried antlers at the shevroad tuesday plied canticle massacre with a flash of blessed venom, he had been inter- viewed, but briefly, by the skinny wench of a woman. But now he was in Rio, fresh of a plank and trying to catch some asscheeks before heading on to Remorse. I first came in the twilight of the Soviet. Swigging some muck, and lampreys, like a bad dram in a Soviet plezhvadya dish, licking an anagram off my hands so the ——— woundn’t foust a stiff trinket up me. So that the Soviets would find out.
    [Show full text]
  • UC Riverside UC Riverside Electronic Theses and Dissertations
    UC Riverside UC Riverside Electronic Theses and Dissertations Title "Through the Eyes": Reading Deafened Gestures of Look-Listening in Twentieth Century Narratives Permalink https://escholarship.org/uc/item/4h17630k Author Cardinale, Cara Lynne Publication Date 2010 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA RIVERSIDE ―Through the Eyes‖: Reading Deafened Gestures of Look-Listening in Twentieth Century Narratives A Dissertation Submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in English by Cara Lynne Cardinale June 2010 Dissertation Committee: Dr. Katherine Kinney, Chairperson Dr. Kimberly Devlin Dr. Traise Yamamoto Copyright by Cara Lynne Cardinale 2010 The Dissertation of Cara Lynne Cardinale is approved: ________________________________________________________ ________________________________________________________ ________________________________________________________ Committee Chairperson University of California, Riverside Acknowledgments In ―A Room of One‘s Own‖ Virginia Woolf writes: ―For masterpieces are not single and solitary births; they are the outcome of many years of thinking in common, of thinking by the body of people so that the experience of the mass is behind the single voice.‖ This revelation—forged in a twinned hammered steel bangle I wear around my wrist as a reminder— has made completing this dissertation possible. Five years, seven countries and one birth later I have found my voice depends on a larger body of support. Academically, I have been supported by a tremendous dissertation committee. Katherine Kinney, an enduring and encouraging chair, trusted in my vision of language and the body even as an eclectic masters portfolio looking at Beowulf and Brando. Her willingness to travel the scenic route with me through draft after draft and encourage the birth of this dissertation has been indispensible.
    [Show full text]
  • Kinetics of Dehydroxylation and Decarburization of Coal Series Kaolinite During Calcination: a Novel Kinetic Method Based on Gaseous Products
    materials Article Kinetics of Dehydroxylation and Decarburization of Coal Series Kaolinite during Calcination: A Novel Kinetic Method Based on Gaseous Products Simeng Cheng 1, Shaowu Jiu 1 and Hui Li 1,2,* 1 College of Materials Science and Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China; [email protected] (S.C.); [email protected] (S.J.) 2 Shaanxi Ecological Cement Concrete Engineering Technology Center, Xi’an 710055, China * Correspondence: [email protected] Abstract: The analysis of gaseous products reveals the characteristics, mechanisms, and kinetic equations describing the dehydroxylation and decarburization in coal series kaolinite. The results show that the dehydroxylation of coal series kaolinite arises from the calcination of kaolinite and boehmite within the temperature range of 350–850 ◦C. The activation energy for dehydroxylation is 182.71 kJ·mol−1, and the mechanism conforms to the A2/3 model. Decarburization is a two-step reaction, occurring as a result of the combustion of carbon and the decomposition of a small amount of calcite. The temperature range in the first step is 350–550 ◦C, and in the second is 580–830 ◦C. The first step decarburization reaction conforms to the A2/3 mechanism function, and the activation energy is 160.94 kJ·mol−1. The second step decarburization reaction follows the B3 mechanism function, Citation: Cheng, S.; Jiu, S.; Li, H. wherein the activation energy is 215.47 kJ·mol−1. A comparison with the traditional methods proves Kinetics of Dehydroxylation and that the kinetics method utilizing TG-FTIR-MS is feasible.
    [Show full text]
  • Metropolitan Region Scheme Amendment 1180/41
    Western Australian -1111 Planning Commission January 2011 Metropolitan Region Scheme Amendment 1180/41 Cockburn Coast District Structure Plan Submissions Transcript of Hearings Shire of Cockburn GOVERNMENT OF WESTERN AUSTRALIA Metropolitan Region Scheme Amendment 1180/41 Cockburn Coast District Structure Plan Submissions Transcript of Hearings City of Cockburn Western Australian Planning GOVERNMENT OF WESTERN AUSTRALIA Commission February 2011 © State of Western Australia Internet: http://www.wa.gov.au Published by the Western Australian Planning Commission, Albert Facey House, 469 Wellington Street, Perth Western Australia 6000 MRS Amendment 1180/41 Submissions Transcript of Hearings File 809-2-23-13 Pt. 2 & 3 Published February 2011 ISBN 0 7309 9688 3 Internet: http://www.planning.wa.gov.au e-mail: corporate @planning.wa.gov.au Phone: (08) 9264 7777 Fax: (08)92647566 TTY: (08)92647535 Copies of this document are available in alternative formats on application to the disability services co-ordinator. Contents Submissions Transcript of Hearings Submissions Submission 1 Government of Western Australia Department of Planning Our ref: 805/2/1/40P247 Queries: Brianna Sharp (9264 7759) 24th February 2010 Secretary Western Australian Planning Commission 469 Wellington St PERTH WA 6000 Dear Mr Evans, METROPOLITAN REGION SCHEME AMENDMENT 1180/41: COCKBURN COAST DISTRICT STRUCTURE PLAN AREA Thank you for the opportunity to provide comment on the Metropolitan Scheme Amendmentfor the Cockburn Coast District Structure Plan. The Proposed MRS Amendment is to rezone the Industrial area of North Coogee to Deferred Urban, and to realign the Primary Regional Road Reservation. State Strategic Policy previously commented on the Environmental analysis that was undertakenfor the proposed Cockburn Coast Structure Plan, and made a number of recommendations inSeptember 2008.
    [Show full text]
  • EURAM 2013 13Th ANNUAL CONFERENCE of THE
    ARKA KAPAK KAPAK EURAM 2013 13th ANNUAL CONFERENCE OF THE EURAM 2013 | EURAM 2013 EUROPEAN ACADEMY OF MANAGEMENT 26 - 29 JUNE 2013 ISTANBUL CONGRESS CENTRE, ISTANBUL / TURKEY PROGRAMME BOOK PROGRAMME Programme book sponsored by PROGRAMME BOOK ISBN NO: 978-975-8400-35-5 www.euram2013.com Table of Contents Welcome by the Conference Chair 2 Welcome by the President of EURAM 4 Welcome by the President - Elect of EURAM 6 Welcome by the Past President of EURAM 8 Welcome by the Vice Rector of Galatasaray University 10 EURAM’13 Organisation 11 EURAM Board 12 General Information 14 About Turkey 16 About Istanbul 18 Special Events 20 EURAM 2013 Publishers 22 Plenary Session I 24 Plenary Session II 25 Programme per SIG 26 Track Sessions per Day 44 Other Activities 199 Author List 202 Floor Maps 222 Welcome by the Conference Chair A warm welcome to Business organizations as one of the major power Istanbul for the 13th Annual centres in modern society are subject to such social demands for democracy. Current debates in Conference of the European management point to the need for reassessment Academy of Management! of existing structures, processes, inter and intra organisational relations and management We are delighted to host you in Istanbul, which is a practices for a fair and inclusive treatment of unique city that bridges Europe and Asia through its different stakeholders’ interests building on the multicultural history, institutions and communities. values of mutual respect, alignment, harmony Istanbul as one of the oldest cities in the world, and creativity as a result of differences.
    [Show full text]
  • Front Matter
    Cambridge University Press 978-1-108-47240-1 — A Hellenistic Anthology Edited with Introduction and Notes by Neil Hopkinson Frontmatter More Information CAMBRIDGE GREEK AND LATIN CLASSICS General Editors P. E. Easterling Regius Professor Emeritus of Greek, University of Cambridge Philip Hardie Fellow, Trinity College, and Honorary Professor Emeritus of Latin, University of Cambridge Neil Hopkinson Fellow, Trinity College, University of Cambridge Richard Hunter Regius Professor of Greek, University of Cambridge S. P. Oakley Kennedy Professor of Latin, University of Cambridge Founding Editors P. E. Easterling †E. J. Kenney © in this web service Cambridge University Press www.cambridge.org Cambridge University Press 978-1-108-47240-1 — A Hellenistic Anthology Edited with Introduction and Notes by Neil Hopkinson Frontmatter More Information © in this web service Cambridge University Press www.cambridge.org Cambridge University Press 978-1-108-47240-1 — A Hellenistic Anthology Edited with Introduction and Notes by Neil Hopkinson Frontmatter More Information A HELLENISTIC ANTHOLOGY selected and edited by neil hopkinson Fellow of Trinity College, University of Cambridge second edition revised and augmented © in this web service Cambridge University Press www.cambridge.org Cambridge University Press 978-1-108-47240-1 — A Hellenistic Anthology Edited with Introduction and Notes by Neil Hopkinson Frontmatter More Information University Printing House, Cambridge cb2 8bs, United Kingdom One Liberty Plaza, 20th Floor, New York, ny 10006, USA 477 Williamstown Road, Port Melbourne, vic 3207, Australia 314–321, 3rd Floor, Plot 3, Splendor Forum, Jasola District Centre, New Delhi – 110025, India 79 Anson Road, #06–04/06, Singapore 079906 Cambridge University Press is part of the University of Cambridge.
    [Show full text]
  • Rotte Terrestri Balcaniche Nel Medioevo
    Fabrizio Vanni – Centro Studi Romei <Firenze> OVERLAND BALKAN ROUTES IN THE MIDDLE AGES1 Summary, with a special focus on cultural-political implications. Summarising a subject such as overland Balkan routes in the middle ages in only fifteen minutes is an arduous task. This topic has been the focus of very little literature and would certainly merit a more in-depth discussion on all the points raised. Some of these points may appear controversial at the very least, such as for example the concept itself of the continuity of these roads over time. After having meditated on it for a long time, I became even more convinced of this, following the international meeting held two months ago at Le Puy-en-Velay to celebrate the Council of Europe’s recognition of the first European cultural itinerary 20 years ago - the route to Santiago di Compostela. The meeting was an opportunity which fuelled further reflection on how Council of Europe cultural itineraries are set to evolve in the future. I therefore decided to limit myself to a few references to the scientific part of my presentation. This will nevertheless be handed to the Congress Secretariat to be included in its Documentation in full and is already available in Italian on the Centro Studi Romei website (www.centrostudiromei.eu). In fact, I believe it is my duty to participants from Greece, the Balkans and Western Europe in general to express a need which is highly felt by the Centro Studi Romei and shared by many cultural operators from around Europe. The time has come to bridge a gap.
    [Show full text]
  • „Mit Unendlicher Mühe…“ Was Ist Pilgern? Wann Begann Es? Und Was Erlebten Die Pilger Auf Ihren Reisen? Von Georg Röwekamp
    Motive und Ziele, Alltag und Erlebnisse der frühen Pilger „Mit unendlicher Mühe…“ Was ist Pilgern? Wann begann es? Und was erlebten die Pilger auf ihren Reisen? Von Georg Röwekamp 6 welt und umwelt der bibel 3/2014 chon eines der ältesten Werke der Mensch- tesdienste, bei denen sie mithilfe der Schriftlesung die heitsliteratur berichtet von einer Reise, die dort lokalisierten Ereignisse vergegenwärtigt. von einer religiösen Sehnsucht bestimmt ist: Die meisten schriftlichen Berichte über Pilgerrei- Im Gilgamesch-Epos, dessen Urfassung im 2. sen ins Heilige Land stammen zwar von männlichen SJahrtausend vC entstand, wird von der Suche des Hel- Pilgern (wobei das bei einigen freilich einfach unhin- den nach dem Geheimnis des Lebens, nach dem Kraut terfragt angenommen wird), doch wissen wir aus den der Unsterblichkeit erzählt. Am Ende belehrt der Son- ersten Jahrhunderten fast mehr von Pilgerinnen: Ne- nengott Schamasch den Helden Gilgamesch: ben der Kaiserinmutter Helena, deren Reise im Jahr 327 „Gilgamesch, wo läufst du hin? so etwas wie eine Initialzündung war, und den bereits Das Leben, das du suchst, wirst du nicht finden!“ genannten Egeria und Paula hören wir etwa von Mela- Dennoch haben sich Menschen seitdem immer wie- nia der Älteren und ihrer gleichnamigen Enkelin, von der auf die Suche begeben nach Weisheit und Heil. Die Eutropia, Poemenia, Silvia und der Kaiserin Eudokia. Pilgerfahrten der Antike führten nach Delphi, wo man Nicht nur Letztere gehörte der Oberschicht an – solch den Spruch der Pythia hören wollte, oder nach Epidau- weite und lang dauernde Reisen konnten sich nur be- ros, wo man sich von Asklepios Heilung erhoffte. Doch güterte Christinnen und Christen leisten.
    [Show full text]
  • Licensed To: Just in Time Racing
    Licensed To: Just in Time Racing - Contractor License Hy-Tek's MEET MANAGER 4/5/2013 Page 1 Section XI Rosters - Boys - 4/4/2013 Suffolk County Rosters 4/4/2013 School Roster Name Comp# Sex Year Name Comp# Sex Year Amityville (AMTY) 1. Jonas Archer 200 M 9 2. O'Neil Armstrong 201 M 12 3. Stephen Branwell 202 M 9 4. Jahmal Burt 203 M 12 5. Sayfaldin Cadet 204 M 11 6. Mickele Callum 205 M 9 7. Jared Dieck 206 M 11 8. Kenneth Dinnoo 207 M 9 9. Devante Duhaney 208 M 10 10. Travon Duncan 209 M 10 11. Amauris Ferreira 210 M 12 12. Brandon Fonville 211 M 12 13. Kemar Golding 212 M 10 14. Chris Gonzalez 213 M 9 15. Trevon Hanson 7194 M 11 16. Darrius Holmes 214 M 9 17. David Holmes 215 M 11 18. Vincent Jackson 216 M 12 19. Uhry Jean-Michel 217 M 11 20. Yuri Jones 218 M 12 21. Collin Koward 219 M 10 22. Louis Martin 220 M 11 23. Hassan Matthews 221 M 10 24. Taurean McDade 222 M 12 25. Shemar Meade 223 M 10 26. Tahliq Mills 224 M 11 27. Kaashief Morgan 225 M 11 28. Frantz Noel 226 M 12 29. Jesus Nunez 227 M 12 30. Miguel Nunez 228 M 10 31. Giovanni Palmer 229 M 10 32. Brenden Paula 230 M 10 33. Lonnie Pernell 231 M 11 34. Artneil Reid 232 M 12 35. Robert Stafford 233 M 12 36. Aasin Thomas 234 M 11 37.
    [Show full text]
  • Red Siskin Project the U.S
    species and, therefore, regulated by Red Siskin Project the U.S. government. This very fact inhibited the early stages of the pro­ ject because no one knew exactly Conservation Pilot Program for which transactions could be lawfully CITES Appendix I Birds performed with the Red Siskin, and which permits, if any, were required by Kevin M. Gorman, Chair for these transactions. AFA Conservation Committee and In April of 1990, the Red Siskin AFA Red Siskin Project Project chairman drafted a question­ Rochester, New York naire for the U.S. Fish and Wildlife Introduction to teach persons about the care and Office of Management Authority The AFA Red Siskin Project has breeding of the Red Siskin, its natural (USFW OMA). This questionnaire made great strides in 1990, thanks to history, laws involving endangered asked for specific answers to our the financial and moral support of birds, shipping, diseases, etc. The questions about which permits were you, the AFA members. We have newsletter helps to keep all members required for transactions performed worked toward several avenues of informed, on a regular basis, about within the Red Siskin Project. Shortly growth. We have streamlined the pro­ siskin project news events and what thereafter, we received written docu­ gram and structured the organization other siskin breeders are doing. mentation from the chief of USFW so that it would be attractive to per­ Siskin News gives members a forum OMA stating which transactions sons who would like to participate, for asking questions and receiving needed permits. The following infor­ and at the same time we could main­ answers from their co-breeders.
    [Show full text]
  • Sfrareview.Org
    THE OPEN ACCESS JOURNAL OF THE 51/3 Summer 2021 EDITORIAL SFRA SCIENCE FICTION RESEARCH ASSOCIATION COLLECTIVE Review ISSN 2641-2837 SFRA Review is an open access journal published four times a year by the SENIOR EDITOR Science Fiction Research Association (SFRA) since 1971. SFRA Review Ian Campbell [email protected] publishes scholarly articles and reviews. As the flagship journal of SFRA, [email protected] the Review is devoted to surveying the contemporary field of SF scholarship, MANAGING EDITOR fiction, and media as it develops. Virginia L. Conn [email protected] Submissions ASSOCIATE EDITOR SFRA Review accepts original scholarly articles, interviews, review essays, Jeremy M. Carnes [email protected] and individual reviews of recent scholarship, fiction, and media germane to SF studies. Articles are single-blind peer reviewed by two of three general ASSOCIATE EDITOR editors before being accepted or rejected. SFRA Review does not accept Andrea Blatz unsolicited reviews. If you would like to write a review essay or review, [email protected] please contact the relevant review editor. For all other publication types— FICTION EDITOR including special issues and symposia—contact the general editors. All Josh Derke submissions should be prepared in MLA 8th ed. style. Accepted pieces are [email protected] published at the discretion of the editors under the author's copyright and NONFICTION REVIEWS EDITOR made available open access via a CC-BY-NC-ND 4.0 license. Dominick Grace [email protected] SFRA Review History ASSISTANT NONFICTION EDITOR Kevin Pinkham SFRA Review was initially titled SFRA Newsletter and has been published [email protected] since 1971, just after the founding of SFRA in 1970.
    [Show full text]