Diptera) Endemic to Madeira – Three New Terricolous Species of Spelobia and Pullimosina
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2019 ACTA ENTOMOLOGICA 59(1): 107–124 MUSEI NATIONALIS PRAGAE doi: 10.2478/aemnp-2019-0009 ISSN 1804-6487 (online) – 0374-1036 (print) www.aemnp.eu RESEARCH PAPER First Sphaeroceridae (Diptera) endemic to Madeira – three new terricolous species of Spelobia and Pullimosina Jindřich ROHÁČEK Department of Entomology, Silesian Museum, Nádražní okruh 31, CZ-746 01 Opava, Czech Republic; e-mail: [email protected] Accepted: Abstract. Three new species of Diptera (Sphaeroceridae: Limosininae) from the Madeira 28th February 2019 Island (Portugal), viz. Spelobia polymorpha sp. nov., Pullimosina (Pullimosina) kesoni sp. Published online: nov., and P. (P.) dorae sp. nov. are described (both sexes), illustrated and their relationships, 13th March 2019 biology and distribution discussed. All these species are terricolous inhabitants of the leaf-litter stratum of the original Madeiran laurel forests (Laurisilva) and because of their poor fl ying ability they are considered endemic to Madeira. None of these species have relatives among endemic species of Sphaeroceridae known from other Macaronesian archipelagos inasmuch as the endemic species of Canary Islands and Azores belong to different genera of Limosininae. Key words. Diptera, Sphaeroceridae, Limosininae, Spelobia, Pullimosina, biology, new species, relationships, taxonomy, distribution, Madeira Zoobank: http://zoobank.org/urn:lsid:zoobank.org:pub:E5CFBE8E-3143-4E9B-AF74-713883BE737D © 2019 The Authors. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Licence. Introduction (1982, 1983a) and ROHÁČEK & MARSHALL (1986), largely As for other groups of two-winged fl ies (Diptera), the resulting from revision of Frey’s material now deposited acalyptrate family Sphaeroceridae has also received some in the Natural History Museum, Helsinki. Trachyopella attention in the Madeiran archipelago (Portugal). Although (Nudopella) hem Roháček & Marshall, 1986 was partly no representative of this family has been recorded from described from Madeiran specimens (paratypes). A total islands Porto Santo, Desertas and/or Selvagens the sphae- of 27 valid species have been recorded from Madeira as rocerid fauna of Madeira Is. is comparatively well known. catalogued by ROHÁČEK et al. (2001) and CARLES-TOLRÁ & BECKER (1908) was the fi rst to report on Sphaeroceridae BÁEZ (2002) but some of these records were considered from Madeira, recording a total of 12 species from this doubtful (see ROHÁČEK et al. 2001). Although four species island, two of which were described as new to science, were described from Madeira none of them is endemic to viz. Olina ferruginea Becker, 1908 and Limosina eximia the island and only two of them remain valid taxa, viz. Becker, 1908. However, DUDA (1918, 1925, 1938), who Spinilimosina brevicostata, Trachyopella hem. Most re- revised material in Becker’s collection, subsequently sy- cently, ROHÁČEK (2007) presented results of the study of nonymized these two species with Lotophila atra (Meigen, material of Sphaeroceridae obtained on a collecting trip 1930) and Trachyopella atomus (Rondani, 1880) respec- to Madeira in 2003. During this fi eld work a total of 39 tively. DUDA (1918) also rectifi ed some other of Becker’s species were found; 20 species were added to the dipterous misidentifi cations and recorded a few additional species fauna of Madeira, but four of them, considered to be new, from his material including Spinilimosina brevicostata remain unnamed. Based on critical evaluation of all pre- (Duda, 1918), described also from a syntype from Madeira. vious records ROHÁČEK (2007) deleted 4 species from the FREY (1949) studied a rich collection of Diptera obtained list of Sphaeroceridae of Madeira due to misidentifi cation on his expedition to Madeira and presented a list of 22 and confi rmed a total of 43 species reliably occurring on species of Sphaeroceridae based on his own material and the island. BECKER’s (1908) records but omitted those published by The present paper is aimed at description of two new DUDA (1918). Much latter, some additional species of species (one of Spelobia and one of Pullimosina) recognized Sphaeroceridae were published from Madeira by ROHÁČEK already by ROHÁČEK (2007) plus an additional new Pulli- Rohacek.indd 107 13.3.2019 10:52:03 108 ROHÁČEK: First Sphaeroceridae endemic to Madeira – three new Spelobia and Pullimosina (Diptera) mosina species found in the material collected by means of the antenna), index t2 : mt2 (= ratio of length of mid tibia : pitfall traps during the research of the laurel forest fauna length of mid basitarsus), wing length (from wing base to performed by the staff of the University of Madeira. All wing tip), wing width (maximum width), C-index (Cs2 : these species were only found in the Laurisilva ecosystem Cs3) (= ratio of length of 2nd costal sector : length of 3rd and all seem to be terricolous animals living under decaying costal sector) and index rm\dm-cu : dm-cu (= ratio of length fallen leaves of laurel trees. With addition of these species, of section between rm and dm-cu on discal cell : length of the Madeiran fauna of Sphaeroceridae comprises now 44 dm-cu). If available a total of 10 males and 10 females (3 species but 2 of them (one belonging to the subgenus Ki- smallest, 4 medium, 3 largest examined specimens of each mosina Roháček, 1983 of Phthitia Enderlein, 1938 and one sex) of each species were measured; in species with fewer to subgenus Allolimosina Roháček, 1983 of Minilimosina specimens available, all were measured. Roháček, 1983, see ROHÁČEK 2007) remain unnamed becau- Presentation of faunistic data. Label data of prima- se the available material is unsuffi cient for their description. ry-type specimens are presented strictly verbatim, includ- ing information on form and colour of all associated labels. Material and methods Data from paratypes of the new species are standardized and presented in full. Phenological and other biological Material. The material examined is deposited in three information obtained from the material examined are institutional collections as follows: summarized in the Biology paragraph. NMPC Národní muzeum, Praha, Czech Republic; Nomenclature follows that used in the World catalog SMOC Slezské zemské muzeum, Opava, Czech Republic; of Sphaeroceridae (ROHÁČEK et al. 2001) and its update UMa Dpto. Biologie, Universidade da Madeira, Funchal, Madeira, Portugal. (MARSHALL et al. 2011). Methods. Specimens have been studied, drawn and measu- Morphological terminology follows that used for red using two types of binocular stereoscopic microscopes Sphaeroceridae by ROHÁČEK (1998) in the Manual of Pa- (Reichert, Olympus). During the course of this work a series laearctic Diptera and, most recently, in ROHÁČEK (2016) of specimens originally preserved in ethanol were dried including terms of the male hypopygium. The “hinge” and mounted on pinned triangular cards. Abdomens of a hypothesis of the origin of the eremoneuran hypopygium, number of specimens were detached (indicated by “genit. re-discovered and documented by ZATWARNICKI (1996), is prep.” in type material examined), cleared by boiling several followed here. Some of the alternate terms for parts of the minutes in a 10% solution of potassium hydroxide (KOH) male genitalia (emanating from other hypotheses) are as in water, then neutralized in a 10% solution of acetic acid follows (the preferred terms are listed fi rst): aedeagus (= phallus), ejacapodeme (= ejaculatory apodeme), epandrium (CH3COOH) in water, washed in water and subsequently transferred to glycerine. Postabdominal structures were dis- (= periandrium), gonostylus (= surstylus), medandrium sected and examined in a drop of glycerine under binocular (= bacilliform sclerite, subepandrial, intraepandrial or microscopes, detailed examinations of genital structures intraperiandrial sclerite), phallapodeme (= aedeagal apo- were performed with a compound microscope (JENAVAL). deme), phallophore (= basiphallus), postgonite (= gonite, After examination, all dissected parts were put into small paramere). Morphological terms of the male terminalia are plastic tubes containing glycerine, sealed with hot forceps depicted in Figs 13–18, 32, those of the female terminalia in and pinned below the respective specimen or put in a vial Figs 19, 21, 23, 46. Abbreviations of morphological terms with the ethanol-preserved specimen. used in text and illustrations are listed below. Drawing techniques and photography. Legs were Abbreviations of morphological terms used in text and/ drawn on squared paper using a Reichert binocular or fi gures: A1 anal vein microscope with an ocular screen. Details of the male and ac acrostichal (seta) female genitalia were drawn by means of Abbe’s drawing ads additional (setulae) on frons apparatus on a compound microscope (JENAVAL) at lar- asc additional sclerite ger magnifi cation (130–500×). Wings were photographed C costa on the same microscope with an attached digital camera ce cercus Cs , Cs 2nd, 3rd costal sector (Nikon COOLPIX 4500). Whole adult specimens and their 2 3 CuA1 cubitus details were photographed by M. Tkoč (National Museum, dc dorsocentral (seta) Praha) using a Canon 5DSR digital camera with a Nikon dm discal medial cell CFI Plan 10×/0.25NA 10.5mm WD objective attached to dm-cu discal medial-cubital (= posterior, tp) cross-vein an EF 70–200mm f/4L USM lens. The specimens were dp distiphallus ea ejacapodeme repositioned upwards between each exposure using a ep epandrium WeMacro Rail (http://www.wemacro.com/, each step