Product datasheet Anti-PNN/DRSP Antibody Catalog Number: A01590-2

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-PNN/DRSP Antibody Name PNN Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA Immunogen E.coli-derived human PNN/DRSP recombinant (Position: A2-L241). Purification Immunogen affinity purified. Observed MW 82KD Dilution Ratios Western blot: 1:500-2000 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Pinin is a protein that in humans is encoded by the PNN gene. By yeast 2-hybrid analysis of HeLa cells, followed by sequence analysis, it was found that epitope-tagged human PNN interacted with the serine/arginine (SR)-rich SRP75 (SRSF4; 601940), SRM300 (SRRM2; 606032), and SRRP130 (PNISR; 616653). Truncation analysis revealed that the polyserine/RS domain of PNN and flanking sequences participated in binding to these SR proteins. The 4 proteins colocalized in the nucleus of HCE-T cells, and overexpression of any of these proteins affected the distribution of the others between nuclear speckles and nucleoplasm.

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1 / 2 Product datasheet Anti-PNN/DRSP Antibody Catalog Number: A01590-2

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. Flow cytometry analysis of anti- PNN analysis of HEPG2 cell antibody (A01590-2).The (1x106) DyLight 488 sample well of each lane conjugated goat anti- was loaded with 50ug of rabbit IgG(blue) was used sample under reducing as secondary conditions. Lane 1: antibody.Isotype control Human HELA whole cell antibody (Green line) was lysates, Lane 2: Human rabbit IgG DyLight 488. HEPG2 whole cell lysates, Unlabelled sample (Red Lane 3: Human HL-60 line). whole cell lysates, Lane 4: Human K562 whole cell lysates, Lane 5: Rat liver tissue lysates, Lane 6: Rat kidney tissue lysates, Lane 7: Mouse Neuro-2a whole cell lysates. Use rabbit anti- PNN 1:1000, probed with a goat anti- rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PNN at approximately 82KD. The expected band size for PNN is at 82KD.

Figure 3. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).

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