Enumeration and Identification of Microflora in “Leben”, a Traditional Tunisian Dairy Beverage
International Food Research Journal 24(3): 927-932 (June 2017) Journal homepage: http://www.ifrj.upm.edu.my Enumeration and identification of microflora in “Leben”, a traditional Tunisian dairy beverage *Samet-Bali, O., Felfoul, I., Lajnaf, R., Attia, H. and Ayadi, M.A. Département de biologie, Laboratoire Valorisation, Analyse et Sécurité des Aliments (LAVASA), Ecole Nationale d’Ingénieurs de Sfax, Route de Soukra, B.P.W, 3038 Sfax, Tunisia Article history Abstract Received: 15 April 2016 The microflora involved in production of Leben, a Tunisian traditional fermented cow milk Received in revised form: product, were enumerated and identified. 15 samples of traditional Leben were analyzed. Total 10 May 2016 viable microorganisms, lactic acid bacteria (LAB), yeasts and moulds, and coliforms were Accepted: 17 May 2016 enumerated. A total of 45 LAB and 30 yeast isolates were isolated from the 15 Leben samples and identified by API 50 CHL and API 20C AUX identification systems, respectively. The LAB counts were 7.8 log10 CFU/mL, while yeast and mould counts were relatively lower (4.7 Keywords log10 CFU/ml). Low coliform numbers were encountered (1.8 log10 CFU/ml). The LAB species were identified as Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris and Fermented cow milk Leuconostoc mesenteroides. The isolated yeasts were identified as Candida krusei, Candida Lactic acid bacteria tropicalis and Candida lusitania. The most frequently isolated species was found to be Yeasts Lactococcus lactis subsp. lactis (28% of total isolates), followed by Lactococcus lactis subsp. Identification cremoris (20%) and Candida krusei (18%). © All Rights Reserved Introduction product fermentation.
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