4/8/2020 Mapping Genomes - Genomes - NCBI Bookshelf
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Brown TA. Genomes. 2nd edition. Oxford: Wiley-Liss; 2002.
Chapter 5 Mapping Genomes
Learning outcomes When you have read Chapter 5, you should be able to:
Explain why a map is an important aid to genome sequencing
Distinguish between the terms ‘genetic map’ and ‘physical map’
Describe the different types of marker used to construct genetic maps, and state how each type of marker is scored
Summarize the principles of inheritance as discovered by Mendel, and show how subsequent genetic research led to the development of linkage analysis
Explain how linkage analysis is used to construct genetic maps, giving details of how the analysis is carried out in various types of organism, including humans and bacteria
State the limitations of genetic mapping
Evaluate the strengths and weaknesses of the various methods used to construct physical maps of genomes
Describe how restriction mapping is carried out
Describe how fluorescent in situ hybridization (FISH) is used to construct a physical map, including the modifications used to increase the sensitivity of this technique
Explain the basis of sequence tagged site (STS) mapping, and list the various DNA sequences that can be used as STSs
Describe how radiation hybrids and clone libraries are used in STS mapping