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Physical Mapping Technologies for the Identification and Characterization of Mutated Genes Contributing to Crop Quality

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Physical Mapping Technologies for the Identification and Characterization of Mutated Genes Contributing to Crop Quality to Crop Quality for the Identification for the Identification and Characterization of Mutated Genes Contributing Physical Mapping Technologies Physical Mapping Technologies IAEA-TECDOC-1664 spine: 7,75 mm - 120 pages IAEA-TECDOC-1664 n PHYSICAL MAPPING TECHNOLOGIES FOR THE IDENTIFICATION AND CHARACTERIZATION OF MUTATED GENES CONTRIBUTING TO CROP QUALITY VIENNA ISSN 1011–4289 ISBN 978–92–0–119610–1 INTERNATIONAL ATOMIC AGENCY ENERGY ATOMIC INTERNATIONAL Physical Mapping Technologies for the Identification and Characterization of Mutated Genes Contributing to Crop Quality The following States are Members of the International Atomic Energy Agency: AFGHANISTAN GHANA NORWAY ALBANIA GREECE OMAN ALGERIA GUATEMALA PAKISTAN ANGOLA HAITI PALAU ARGENTINA HOLY SEE PANAMA ARMENIA HONDURAS PARAGUAY AUSTRALIA HUNGARY PERU AUSTRIA ICELAND PHILIPPINES AZERBAIJAN INDIA POLAND BAHRAIN INDONESIA PORTUGAL BANGLADESH IRAN, ISLAMIC REPUBLIC OF QATAR BELARUS IRAQ REPUBLIC OF MOLDOVA BELGIUM IRELAND ROMANIA BELIZE ISRAEL RUSSIAN FEDERATION BENIN ITALY SAUDI ARABIA BOLIVIA JAMAICA BOSNIA AND HERZEGOVINA JAPAN SENEGAL BOTSWANA JORDAN SERBIA BRAZIL KAZAKHSTAN SEYCHELLES BULGARIA KENYA SIERRA LEONE BURKINA FASO KOREA, REPUBLIC OF SINGAPORE BURUNDI KUWAIT SLOVAKIA CAMBODIA KYRGYZSTAN SLOVENIA CAMEROON LATVIA SOUTH AFRICA CANADA LEBANON SPAIN CENTRAL AFRICAN LESOTHO SRI LANKA REPUBLIC LIBERIA SUDAN CHAD LIBYAN ARAB JAMAHIRIYA SWEDEN CHILE LIECHTENSTEIN SWITZERLAND CHINA LITHUANIA SYRIAN ARAB REPUBLIC COLOMBIA LUXEMBOURG TAJIKISTAN CONGO MADAGASCAR THAILAND COSTA RICA MALAWI THE FORMER YUGOSLAV CÔTE D’IVOIRE MALAYSIA REPUBLIC OF MACEDONIA CROATIA MALI TUNISIA CUBA MALTA TURKEY CYPRUS MARSHALL ISLANDS UGANDA CZECH REPUBLIC MAURITANIA UKRAINE DEMOCRATIC REPUBLIC MAURITIUS UNITED ARAB EMIRATES OF THE CONGO MEXICO UNITED KINGDOM OF DENMARK MONACO GREAT BRITAIN AND DOMINICAN REPUBLIC MONGOLIA NORTHERN IRELAND ECUADOR MONTENEGRO EGYPT MOROCCO UNITED REPUBLIC EL SALVADOR MOZAMBIQUE OF TANZANIA ERITREA MYANMAR UNITED STATES OF AMERICA ESTONIA NAMIBIA URUGUAY ETHIOPIA NEPAL UZBEKISTAN FINLAND NETHERLANDS VENEZUELA FRANCE NEW ZEALAND VIETNAM GABON NICARAGUA YEMEN GEORGIA NIGER ZAMBIA GERMANY NIGERIA ZIMBABWE The Agency’s Statute was approved on 23 October 1956 by the Conference on the Statute of the IAEA held at United Nations Headquarters, New York; it entered into force on 29 July 1957. The Headquarters of the Agency are situated in Vienna. Its principal objective is “to accelerate and enlarge the contribution of atomic energy to peace, health and prosperity throughout the world’’. IAEA-TECDOC-1664 PHYSICAL MAPPING TECHNOLOGIES FOR THE IDENTIFICATION AND CHARACTERIZATION OF MUTATED GENES CONTRIBUTING TO CROP QUALITY INTERNATIONAL ATOMIC ENERGY AGENCY VIENNA, 2011 COPYRIGHT NOTICE All IAEA scientific and technical publications are protected by the terms of the Universal Copyright Convention as adopted in 1952 (Berne) and as revised in 1972 (Paris). The copyright has since been extended by the World Intellectual Property Organization (Geneva) to include electronic and virtual intellectual property. Permission to use whole or parts of texts contained in IAEA publications in printed or electronic form must be obtained and is usually subject to royalty agreements. Proposals for non-commercial reproductions and translations are welcomed and considered on a case-by-case basis. Enquiries should be addressed to the IAEA Publishing Section at: Sales and Promotion, Publishing Section International Atomic Energy Agency Vienna International Centre PO Box 100 1400 Vienna, Austria fax: +43 1 2600 29302 tel.: +43 1 2600 22417 email: [email protected] http://www.iaea.org/books For further information on this publication, please contact: Plant Breeding and Genetics Section International Atomic Energy Agency Vienna International Centre PO Box 100 1400 Vienna, Austria Email: [email protected] PHYSICAL MAPPING TECHNOLOGIES FOR THE IDENTIFICATION AND CHARACTERIZATION OF MUTATED GENES CONTRIBUTING TO CROP QUALITY IAEA, VIENNA, 2011 IAEA-TECDOC-1664 ISBN 978-92-0-119610-1 ISSN 1011-4289 © IAEA, 2011 Printed by the IAEA in Austria September 2011 FOREWORD The improvement of quality traits in food and industrial crops is an important breeding objective for both developed and developing countries in order to add value to the crop and thereby increasing farmers’ income. It has been well established that the application of mutagens can be a very important approach for manipulating many crop characteristics including quality. While mutation induction using nuclear techniques such as gamma irradiation is a power tool in generating new genotypes with favourable alleles for improving crop quality in plant breeding, a more thorough understanding of gene expression, gene interactions, and physical location will improve ability to manipulate and control genes, and directly lead to crop improvement. Physical mapping technologies, molecular markers and molecular cytogenetic techniques are tools available with the potential to enhance the ability to tag genes and gene complexes to facilitate the selection of desirable genotypes in breeding programmes, including those based on mutation breeding. This Coordinated Research Project (CRP) on ‘Physical Mapping Technologies for the Identification and Characterization of Mutated Genes Contributing to Crop Quality’ was conducted under the overall IAEA project objective of ‘Identification, Characterization and Transfer of Mutated Genes’. The specific objectives of the CRP were to assist Member States in accelerating crop breeding programmes through the application of physical mapping and complementary genomic approaches, and the characterization and utilization of induced mutants for improvement of crop quality. The IAEA-TECDOC describes the success obtained in the application of molecular cytology, molecular markers, physical mapping and mutation technologies since the inception of the CRP in 2003. The CRP also resulted in two book chapters, 35 peer reviewed papers, 25 conference proceedings, one PhD thesis, and 22 published abstracts. In addition, thirteen sequences were submitted to the worldwide Genbank.The CRP was initiated by S. Nielen, continued by M. Miranda, both formerly of the Joint FAO/IAEA Division. The IAEA Officer responsible for this IAEA- TECDOC was Y. Lokko. EDITORIAL NOTE The papers in these proceedings are reproduced as submitted by the authors and have not undergone rigorous editorial review by the IAEA. The views expressed do not necessarily reflect those of the IAEA, the governments of the nominating Member States or the nominating organizations. The use of particular designations of countries or territories does not imply any judgement by the publisher, the IAEA, as to the legal status of such countries or territories, of their authorities and institutions or of the delimitation of their boundaries. The mention of names of specific companies or products (whether or not indicated as registered) does not imply any intention to infringe proprietary rights, nor should it be construed as an endorsement or recommendation on the part of the IAEA. The authors are responsible for having obtained the necessary permission for the IAEA to reproduce, translate or use material from sources already protected by copyrights. CONTENTS Summary .................................................................................................................................................. 1 Comparative physical mapping of rearranged and normal plant chromosomes by high- resolution FISH and Megabase DNA Techniques ................................................................................... 9 T. Schmidt, D. Dechyeva, C. Fiege, T. Heitkam, G. Menzel, B. Weber, T. Wenke, F. Zakrzewski Exploitation of physical mapping technologies for breeding of canola mutants in oilseed brassicas .... 17 I. Ali, H.M. Ahmad, S.A. Shah, T. Ahmad, K. Rehman, M. Ahmad Marker assisted selection for fiber quality improvement in mutation breeding programme of cotton ............................................................................................ 27 M. Asif, M. Ur-Rahman, Y. Zafar Mutagenesis and physical mapping of genes in crops with small chromosomes .................................. .39 J. Juchmiuk-Kwasniewska, B. Kolano, R. Hasterok, M. Hosiawa, J. Maluszynska Molecular cytogenetics of triticeae polyploids: Lymegrass and wheat x lymegrass hybrids ................ .53 K. Anamthawat-Jónsson Develoment of physical cytogenetic maps for bananas and plantains ................................................... 61 E. Hribova, M. Dolezelova, P. Nemcova, J. Cizkova, L. Schillerova, J. Dolozel Molecular characterization of the grain gelatinization temperature trait in rice (Oryza Sativa L.) ........ 71 S.Q. Shen, X.L. Shu, Q.Y. Shu Physical mapping and relationship of genome-specific repetitive DNA sequence with genes contributing to crop quality in wild rice .......................................................................... 85 J. Chen, Y. Hong, N. Xiao, R. Xia Genomic characterization of the chili peppers (Capsicum, Solanaceae) germplasm by classical and molecular cytogenetics ................................................................................................. 97 E.A. Moscone, M.A. Scaldaferro, M. Grabiele, M.V. Romero, H. Debat, J.G. Seijo, M.C. Acosta, J.R. Daviña, G.E. Barboza, D.A. Ducasse Induction and study of tomato and pepper mutants with high nutritive quality ................................... 105 N. Tomlekova Fluorescent
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