Characterization of the Feeding Behavior of Three Erythroneura Species on Grapevine by Histological and DC-Electrical Penetration Graph Techniques
DOI: 10.1111/eea.12353 Characterization of the feeding behavior of three Erythroneura species on grapevine by histological and DC-electrical penetration graph techniques Julien Saguez1*, Pierre Lemoyne1, Philippe Giordanengo2,3,ChrystelOlivier4, Jacques Lasnier5,YvesMauffette6 & Charles Vincent1 1Agriculture et Agroalimentaire Canada, 430 Boulevard Gouin, Saint-Jean-sur-Richelieu, Quebec J3B 3E6, Canada, 2Universite de Picardie Jules Verne, 33 Rue St Leu, 80039 Amiens Cedex, France, 3Institut Sophia Agrobiotech, UMR 1355 INRA/Universite Nice Sophia Antipolis/7254 CNRS, 400 route des Chappes, 06903 Sophia Antipolis Cedex, France, 4Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada, 5Co-Lab R&D div. Ag-Cord, 655 Rue Delorme, Granby, Quebec J2J 2H4, Canada, and 6UniversiteduQuebec a Montreal, 141 Rue du President-Kennedy, Montreal, Quebec H2X 3Y5, Canada Accepted: 22 July 2015 Key words: mesophyll-feeder, piercing-sucking insect, plant tissues, salivary sheath, stylet penetration, Vitis, xylem, Auchenorrhyncha, Hemiptera, Cicadellidae, Vitaceae, DC-EPG Abstract Feeding behavior of three leafhopper species – Erythroneura vitis (Harris), Erythroneura ziczac (Walsh), and Erythroneura elegantula (Say) (Hemiptera: Cicadellidae) – reared on grapevine, Vitis vinifera L. cv. ‘Seyval blanc’ (Vitaceae), was investigated using histological techniques and DC-electri- cal penetration graphs (DC-EPG). Histological studies revealed that the Erythroneura species induced white stipples on the leaves and that these leafhoppers produced thin salivary sheaths in grapevine leaf tissues. The DC-EPG system allowed the characterization of five waveforms associated with stylet penetration and feeding in leaf tissues. These waveforms were characteristic of feeding phases corre- sponding to epidermis penetration pathway, salivation, and ingestion. We calculated 28 parameters (e.g., number of probes, duration of phases, and time spent in the various tissues) to describe and compare the feeding behavior of the Erythroneura species.
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