MSACL 2014 EU September 1 Austria 2-5, 2014 Salzburg, Con Dence in Results MSACL 2014 EU

Total Page:16

File Type:pdf, Size:1020Kb

MSACL 2014 EU September 1 Austria 2-5, 2014 Salzburg, Con Dence in Results MSACL 2014 EU The Association for MS Mass Spectrometry: ACL Applications to the Clinical Lab MSACL 2014 EU September 2-5, 2014 Salzburg, Austria 1 2014 EU September MSACL Condence in Results MSACL 2014 EU ™ high-performance medical devices for in vitro diagnostic use ™ Prelude MD ™ ™ Endura MD ™ mass spectrometer, and Thermo Salzburg, Austria ™ ClinQuan MD ™ LC-MS for in vitro diagnostic use September 2-5 The 1st Annual European Congress Visit us in booth #12 • st Annual European Congress Program Congress Annual European e licensed for limited use only to Thermo r d parties including Shutterstock, and a r by thi Supported in part by generous contributions from: For in vitro diagnostic use. Not available in all countries. San Jose, CA USA i s Endura MD Mass ISO 13485 Prelude MD HPLC Spectrometer ClinQuan MD Software AD64157-EN 0614S-Rev.B MSACLMASS SPECTROMETRY SOLUTIONS FOR IN2014 VITRO DIAGNOSTICS EU The variety of your clinical research Salzburg, Austria demands a variety of technologies September 2-5 The 1st Annual European Congress You’re not the only one who needs to trust the test results ACCURACY THE FIRST TIME No one analytical technology can adequately reveal everything you need ! GC-Triple Quadrupole to know about your sample. Bruker offers the broadest range of high ! LC-Triple Quadrupole Clinicians and patients count on the most reliable diagnostic answers. We want you to be confident that results are as timely performance, easy-to-use, and expertly supported analytical systems ! LC-QqTOF and accurate as possible. SCIEX Diagnostics offers an in vitro diagnostics solution with exceptional assay performance that helps to overcome any clinical research challenge. Our proven, extremely overcome the limitations of current testing methods. Our newly introduced assays for Vitamin D, Newborn Screening and robust GC-Triple Quads, LC-Triple Quads, Ion Traps and QqTOFs ensure Immunosuppressants accurately measure multiple analytes in a single run, and virtually eliminate the need for re-runs and confidence in your results for microbiology, biomarker discovery, tissue send-outs. And not only are SCIEX Diagnostics solutions backed by over 25 years of leadership in mass spectrometry, imaging, and drugs of abuse or drug monitoring research. they are surprisingly affordable, too. Get the right answer faster, more cost effectively, using the appropriate Find out how SCIEX Diagnostics can help improve your accuracy, turnaround and confidence analytical technology for the job – Bruker’s complementary solutions for at www.sciexdiagnostics.com. clinical research. Come and visit us at MSACL, Booth 10 and 11. Visit us at MSACL, Booth 15 and on the Web at www.bruker.com Diagnostics GC/LC/MS Systems For In Vitro Diagnostic Use. Not available in all countries. © 2014 AB SCIEX. SCIEX is part of AB SCIEX. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Innovation with Integrity IVD-MKT-13-1360-A For research use only. Not for use in diagnostic procedures. MSACL 2014 EU The First Annual European Congress of The Association for Mass Spectrometry: Applications to the Clinical Lab Salzburg, AUSTRIA September 2 - 5, 2014 Salzburg Congress Center The Association is a non-membership, non-profit 501(c)(3) tax-exempt California Corporation with the mission of furthering education in the field of mass spectrometry. Go Mobile! Mobile Program @ https://www.msacl.org/mobile Get Connected with BadgeScanTM. The Official Contact & Lead Capture App of MSACL 2014 EU. 1 MSACL 2015 US San Diego March 28 - April 1 The 7th Annual US Conference on Clinical Mass Spectrometry Travel Award & Podium Submission Deadline: December 4, 2014 Presented by: The Association for MS Mass Spectrometry: ACL Applications to the Clinical Lab Supported by: Table of Contents CORPORATE SPONSORS .................................................................................................................... 4 SCIENTIFIC COMMITTEE ................................................................................................................. 5 GENERAL INFORMATION ............................................................................................................... 17 PRESENTER INFO AND GUIDELINES ........................................................................................... 18 SCHEDULE OVERVIEW .................................................................................................................... 19 TUESDAY ......................................................................................................................... 19 WEDNESDAY ................................................................................................................... 19 THURSDAY ...................................................................................................................... 20 FRIDAY ............................................................................................................................ 21 PLENARY SPEAKER SERIES ........................................................................................................... 23 YOUNG INVESTIGATOR AWARDS ................................................................................................ 25 TRAINEE AWARDS ............................................................................................................................ 26 SHORT COURSE OVERVIEW .......................................................................................................... 27 EXHIBITS SUMMARY ........................................................................................................................ 30 EXHIBITORS ........................................................................................................................................ 31 CORPORATE WORKSHOPS ............................................................................................................. 35 PODIUM PRESENTATIONS .............................................................................................................. 39 POSTER PRESENTATIONS ............................................................................................................... 53 POSTERS BY TOPIC ......................................................................................................... 54 POSTERS: WEDNESDAY .............................................................................................. 67 POSTERS: THURSDAY ................................................................................................. 77 POSTERS: FRIDAY ........................................................................................................ 91 PRESENTER INDEX ........................................................................................................................... 99 MAP: 2ND FLOOR ............................................................................................................................... 100 MAP: 1ST FLOOR - EXHIBIT HALL ................................................................................................ 101 MAP: GROUND FLOOR ................................................................................................................... 102 3 Corporate Sponsors 4 Scientific Committee Please take a moment to acknowledge the members of the Scientific Committee of this inaugural congress who were pivitoal in the development of the Scientific Program. Theodore Alexandrov, PhD University of Bremen, Germany / SCiLS, Germany / UCSD, USA Jean Armengaud, PhD CEA-Marcoule, France Olof Beck, PhD Karolinska Institutet, Sweden Karl-Siegfried Boos, PhD Professor of Clinical Chemistry Institute of Laboratory Medicine, Medical Center of the University, Munich, Germany Uta Ceglarek, PhD, EurClinChem Leipzig University Olivier Gaillot, PhD Centre de Biologie Pathologie, CHU de Lille, France Roland Geyer Tecan Switzerland AG Brian Keevil, PhD Department of Clinical Biochemistry, University Hospital of South Manchester, Manchester, UK Pierre Marquet, MD, PhD University of Limoges, France 5 Oleg Mayboroda, PhD Leiden University Medical Center, The Netherlands Prof. Dr. med. Soren Schubert Ludwig-Maximilians-Universitat (LMU) Munchen Christoph Seger, PhD University Hospital Innsbruck Zoltan Takats, PhD Imperial College, London, UK Prof. Dr. med. Michael Vogeser Institute of Laboratory Medicine, Hospital of the University of Munich, Germany for the working group LC-MS/MS of the DGKL Stefan Zimmermann, MD University Hospital Heidelberg, Germany 6 Geotrichum Fusobacterium Lodderomyces Anaerococcus Colletotrichum Edwardsiella Comamonas Pigmentiphaga Moellerella Nesterenkonia Listonella Udeniomyces Erysipelothrix Escherichia Streptomyces Bartonella Hafnia Terrimonas Pseudoxanthomonas Corynebacterium Streptococcus Aerococcus Saccharothrix Facklamia Schizosaccharomyces Tetragenococcus Lechevalieria Clavibacter Shimwellia Brachybacterium Leclercia Providencia Trabulsiella Xanthobacter Emericella Gardnerella Sporothrix Leuconostoc Pseudoclavibacter Alkalibacillus Debaryomyces Turicella Roseomonas Ruminococcus Scedosporium Dysgonomonas Staphylococcus Peptostreptococcus Paenibacillus Balneatrix Solibacillus Prototheca Cupriavidus Geobacillus Aspergillus Arthrobacter Mesorhizobium Acholeplasma Filobasidium Propioniferax Azohydromonas Chromobacterium Curtobacterium Kloeckera Austwickia Hyphomicrobium Cryptococcus Rummeliibacillus Budvicia Aquincola Enterobacter Sporobolomyces Brevundimonas Capnocytophaga
Recommended publications
  • The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine in This Issue
    June 2021 ISSN 1650-3414 Volume 32 Number 2 Communications and Publications Division (CPD) of the IFCC Editor-in-chief: Prof. János Kappelmayer, MD, PhD Faculty of Medicine, University of Debrecen, Hungary e-mail: [email protected] The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine In this issue Introducing the eJIFCC special issue on “POCT – making the point” Guest editor: Sergio Bernardini 116 Controlling reliability, interoperability and security of mobile health solutions Damien Gruson 118 Best laboratory practices regarding POCT in different settings (hospital and outside the hospital) Adil I. Khan 124 POCT accreditation ISO 15189 and ISO 22870: making the point Paloma Oliver, Pilar Fernandez-Calle, Antonio Buno 131 Utilizing point-of-care testing to optimize patient care James H. Nichols 140 POCT: an inherently ideal tool in pediatric laboratory medicine Siobhan Wilson, Mary Kathryn Bohn, Khosrow Adeli 145 Point of care testing of serum electrolytes and lactate in sick children Aashima Dabas, Shipra Agrawal, Vernika Tyagi, Shikha Sharma, Vandana Rastogi, Urmila Jhamb, Pradeep Kumar Dabla 158 Training and competency strategies for point-of-care testing Sedef Yenice 167 Leading POCT networks: operating POCT programs across multiple sites involving vast geographical areas and rural communities Edward W. Randell, Vinita Thakur 179 Connectivity strategies in managing a POCT service Rajiv Erasmus, Sumedha Sahni, Rania El-Sharkawy 190 POCT in developing countries Prasenjit Mitra, Praveen Sharma 195 In this issue How could POCT be a useful tool for migrant and refugee health? Sergio Bernardini, Massimo Pieri, Marco Ciotti 200 Direct to consumer laboratory testing (DTCT) – opportunities and concerns Matthias Orth 209 Clinical assessment of the DiaSorin LIAISON SARS-CoV-2 Ag chemiluminescence immunoassay Gian Luca Salvagno, Gianluca Gianfilippi, Giacomo Fiorio, Laura Pighi, Simone De Nitto, Brandon M.
    [Show full text]
  • 1995) Borrelia Burgdorferi-Specific T Lymphocytes Induce Severe Destructive Lyme Arthritis
    LTT (Lymphozyten-Proliferationstest, „Lymphozyten-Transformationstest“) Interferon Gamma Test, ELISpot (T-Cell Spot), dem Lymphozytentransformationstest bei Borreliose und bei anderen Infektionskrankheiten Im Lymphozyten-Proliferationstest, auch Lymphozyten-Transformationstest genannt (LTT), wird die Proliferationskapazität von Lymphozyten dargestellt. Der Test dient der Bewertung des Immunstatus oder der Diagnose einer speziellen Sensibilisierung. In the Lymphocyte proliferation assay, also known as lymphocyte transformation test (LTT), the proliferation capacity of lymphocytes is shown. The test is used to evaluate the immune status or the diagnosis of specific sensitization of an object. Im Interferon Gamma Test, dem ELISPOT (Enzyme-Linked ImmunoSpot), auch T- Cellspot genannt, wird durch die Bestimmung der Zytokin – Produktion die Quantität und die Qualität einer T – Zell - Immunantwort zeitnah dokumentiert. In the Interferone Gamma Test, ELISPOT (Enzyme-Linked ImmunoSpot), also called T-Cell spot, represented by the determination of cytokine – Production, the quantity and quality of T - cell - immune response in a timely manner is documented. Lim LC, England DM, DuChateau BK, Glowacki NJ, Schell RF (1995) Borrelia burgdorferi-specific T lymphocytes induce severe destructive Lyme arthritis. Infect. Immun. 63, 1400-1408. Dwyer JM et al. (1979) Behavior of human immunoregulatory cells in culture. L Variables requiring consideration for clinical studies. Clin Exp Immunol 38, 499-513 Johnson C, Dwyer JM (1981) Quantitation of spontaneous suppressor cell activity in human cord blood lymphocytes and their behavior in culture (abstract). Fed Proc 40, 1075 Czerkinsky C, Nilsson L, Nygren H, Ouchterlony O, Tarkowski A (1983) A solid-phase enzyme- linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells.J Immunol Methods 65 (1–2), 109–121.
    [Show full text]
  • Ampath Medical Surveillance Guidelines Metal Chemicals
    AMPATH MEDICAL SURVEILLANCE GUIDELINES METAL CHEMICALS TABLE OF CONTENTS Introduction 3 Definitions 4 Biological monitoring 7 Biological effect monitoring 15 Health program 15 Food handler’s health program 16 Driver’s health program 16 Substance abuse in the workplace 17 Alcohol in the workplace 17 Target organs for alcohol abuse 21 Drugs in the workplace 22 Ampath drugs of abuse screening and confirmatory tests on urine 23 Conditions under which a pathologist will testify in court 23 Chain of custody 24 Hazardous chemical regulations 25 Designing and implementing a program of medical surveillance 25 Assessment of potential exposure 25 Table 3 substances 26 Available tests 27 Test interpretation 31 Ampath Quality Assurance 33 Designing exposure profiles 34 Work related hazardous chemical exposure 36 Chemical exposure: DNA adducts 37 Target organs for chemical exposure 39 Chemical exposure profiles 40 Aniline 40 Acetone 41 Benzene 42 Carbon disulphide 43 Carbon monoxide 44 Cyanide 45 Ethyl benzene 46 NN-dimethylformamide 47 Furfural 48 N-hexane 49 © Ampath Medical Surveillance Guideline 1 Isocyanate 50 Methanol 51 Methyl chloroform 52 Methyl ethyl ketone 53 Methyl isobutyl ketone 54 Nitrobenzene 55 Organophosphorous cholinesterase inhibitors 56 Paraquat 57 Parathion 58 Pentachlorophenol 59 Perchloroethylene 60 Phenol 61 Polycyclic aromatic hydrocarbon(PAH) 62 Styrene 64 Toluene 65 Trichloroethylene 67 Xylene 68 Metals 69 Diagnosis and investigation of occupational exposure to metals; a general review 69 Target organs for metal exposure
    [Show full text]
  • Ampath Desk Reference: Guide to Laboratory Tests
    Ampath Guide to Lab Tests front cover final repro outlines 15 March 2016.
    [Show full text]
  • Abstracts of Poster Presentations
    Abstracts of Poster Presentations To view the full program, please visit the Congress website www.kenes.com/aps Downloaded from lup.sagepub.com at University Library Utrecht on March 17, 2015 loth ktertSic (Qn nsanAtiopuipdAMibocEes PFates 584 ANTIPHOSPHOLIPID ANTIBODIES IN PATIENTS WITH DEVELOPMENT OF ANTIPHOSDPHOLIPID-ANTIBODIES AND INTOLERANCE TO METALS IN THE ORAL CAVITY FACrOR V-INHIBITOR AFTER CIPROFLOXACIN IN TWO CASES H. Kucerova', J. Prochazkovat, I. Janatkova2, J. Bartova', T. Fucikova2 W.Miesbachl, J. Vogte, D. Peez3, B. Buehler', G. Asmelashl', 'Institute ofDental Research, 1st Medical Faculty, 2Institute ofClinical 1. Scharrerl Imnmunology andAllergology, General Faculty Hospital, Prague, 'J W.Goethe-University, Frankfurt, 25t. Vincenz Hospital, Mainz, Czech Republic 3Justus Liebig University, Frankfurt, Germany Dental alloys are not a physiological part of the organism. Metals can cause The development of factor V-inhibitor is very rare, especially in the production of autoantibodies in sensitive individuals as well as other combination with the antiphospholipid (aPL)-antibodies. The present paper undesired side effects. The aim of this presentation is to evaluate the describes two patients with factor V-inhibitor and aPL-antibodies after presence of antiphospholipid antibodies in sera of patients with oral trcatment with ciprofloxacin. Lupus anticoagulants (LA) were assayed discomfort and the occurrence of pathological galvanic currents in the oral according to the criteria of SSC of the ISTH, ACL by an ELISA, factor V- cavity. The patients were diagnosed by measurement of galvanic features inhibitor by the Bethesda method. with the device Odontologic accompanied by the modified method ofblastic Q.Oe.atirti 74 years old was treated with ciprofloxacin for a postoperative transfonnation for metals (Melisa) and by the anamnestic data.
    [Show full text]
  • XXI Fungal Genetics Conference Abstracts
    Fungal Genetics Reports Volume 48 Article 17 XXI Fungal Genetics Conference Abstracts Fungal Genetics Conference Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Fungal Genetics Conference. (2001) "XXI Fungal Genetics Conference Abstracts," Fungal Genetics Reports: Vol. 48, Article 17. https://doi.org/10.4148/1941-4765.1182 This Supplementary Material is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact [email protected]. XXI Fungal Genetics Conference Abstracts Abstract XXI Fungal Genetics Conference Abstracts This supplementary material is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol48/iss1/17 : XXI Fungal Genetics Conference Abstracts XXI Fungal Genetics Conference Abstracts Plenary sessions Cell Biology (1-87) Population and Evolutionary Biology (88-124) Genomics and Proteomics (125-179) Industrial Biology and Biotechnology (180-214) Host-Parasite Interactions (215-295) Gene Regulation (296-385) Developmental Biology (386-457) Biochemistry and Secondary Metabolism(458-492) Unclassified(493-502) Index to Abstracts Abstracts may be cited as "Fungal Genetics Newsletter 48S:abstract number" Plenary Abstracts COMPARATIVE AND FUNCTIONAL GENOMICS FUNGAL-HOST INTERACTIONS CELL BIOLOGY GENOME STRUCTURE AND MAINTENANCE COMPARATIVE AND FUNCTIONAL GENOMICS Genome reconstruction and gene expression for the rice blast fungus, Magnaporthe grisea. Ralph A. Dean. Fungal Genomics Laboratory, NC State University, Raleigh NC 27695 Rice blast disease, caused by Magnaporthe grisea, is one of the most devastating threats to food security worldwide.
    [Show full text]
  • August 2021 Vectorborne Infectious Diseases
    1913.),Culebra Jonas( OilLie Cut, on(1880−1940) canvas, Panama 60 Canal in The x 50 Conquerors in/ 152.4 cm x 127 cm. InfectiousDiseases Vectorborne Image copyright © The Metropolitan Museum of Art, New York, NY, United States. Image source: Art Resource, New York, NY, United States. August 2021 ® Peer-Reviewed Journal Tracking and Analyzing Disease Trends Pages 2008–2250 ® EDITOR-IN-CHIEF D. Peter Drotman ASSOCIATE EDITORS EDITORIAL BOARD Charles Ben Beard, Fort Collins, Colorado, USA Barry J. Beaty, Fort Collins, Colorado, USA Ermias Belay, Atlanta, Georgia, USA Martin J. Blaser, New York, New York, USA David M. Bell, Atlanta, Georgia, USA Andrea Boggild, Toronto, Ontario, Canada Sharon Bloom, Atlanta, Georgia, USA Christopher Braden, Atlanta, Georgia, USA Richard Bradbury, Melbourne, Australia Arturo Casadevall, New York, New York, USA Corrie Brown, Athens, Georgia, USA Benjamin J. Cowling, Hong Kong, China Kenneth G. Castro, Atlanta, Georgia, USA Michel Drancourt, Marseille, France Christian Drosten, Charité Berlin, Germany Paul V. Effler, Perth, Australia Isaac Chun-Hai Fung, Statesboro, Georgia, USA Anthony Fiore, Atlanta, Georgia, USA Kathleen Gensheimer, College Park, Maryland, USA David O. Freedman, Birmingham, Alabama, USA Rachel Gorwitz, Atlanta, Georgia, USA Peter Gerner-Smidt, Atlanta, Georgia, USA Duane J. Gubler, Singapore Stephen Hadler, Atlanta, Georgia, USA Scott Halstead, Arlington, Virginia, USA Matthew J. Kuehnert, Edison, New Jersey, USA Nina Marano, Atlanta, Georgia, USA David L. Heymann, London, UK Martin I. Meltzer, Atlanta, Georgia, USA Keith Klugman, Seattle, Washington, USA David Morens, Bethesda, Maryland, USA S.K. Lam, Kuala Lumpur, Malaysia J. Glenn Morris, Jr., Gainesville, Florida, USA Shawn Lockhart, Atlanta, Georgia, USA Patrice Nordmann, Fribourg, Switzerland John S.
    [Show full text]
  • Immunomodulation of T Helper Cells by Tumor Microenvironment in Oral
    ORIGINAL RESEARCH published: 07 May 2021 doi: 10.3389/fimmu.2021.643298 Immunomodulation of T Helper Cells by Tumor Microenvironment in Oral Cancer Is Associated With CCR8 Expression and Rapid Membrane Edited by: Vitamin D Signaling Pathway Lewis Z. Shi, University of Alabama at Birmingham, 1 ´ 2 3,4,5 1 United States Marco Fraga , Milly Yañez , Macarena Sherman , Faryd Llerena , Mauricio Hernandez 6, Guillermo Nourdin 6, Francisco A´ lvarez 6, Joaqu´ın Urrizola 7, Reviewed by: Ce´ sar Rivera 8, Liliana Lamperti 1,9, Lorena Nova 10, Silvia Castro 1, Omar Zambrano 11, Hongru Zhang, 11 ´ 12 12 12 12 University of Pennsylvania, Alejandro Cifuentes , Leon Campos , Sergio Moya , Juan Pastor , Marcelo Nuñez , 12 12 ´ 1 ´ 13 14 United States Jorge Gatica , Jorge Figueroa , Felipe Zuñiga , Carlos Salomon , Gustavo Cerda , 5 1 15 16 Greg M. Delgoffe, Ricardo Puentes , Gonzalo Labarca , Mabel Vidal , Reuben McGregor 1 University of Pittsburgh, and Estefania Nova-Lamperti * United States 1 Molecular and Translational Immunology Laboratory, Clinical Biochemistry and Immunology Department, Pharmacy Faculty, *Correspondence: Universidad de Concepcio´ n, Concepcio´ n, Chile, 2 Anatomy Pathology Unit and Dental Service, Oral Pathology Department, Estefania Nova-Lamperti Hospital Las Higueras, Talcahuano, Chile, 3 Anatomy Pathology Unit, Hospital Guillermo Grant Benavente and Universidad [email protected]; de Concepcio´ n, Concepcio´ n, Chile, 4 Head and Neck Service, Hospital Guillermo Grant Benavente, Concepcio´ n, Chile, [email protected] 5 Dental
    [Show full text]
  • IUPAC Glossary of Terms Used in Immunotoxicology (IUPAC Recommendations 2012)*
    Pure Appl. Chem., Vol. 84, No. 5, pp. 1113–1295, 2012. http://dx.doi.org/10.1351/PAC-REC-11-06-03 © 2012 IUPAC, Publication date (Web): 16 February 2012 IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)* Douglas M. Templeton1,‡, Michael Schwenk2, Reinhild Klein3, and John H. Duffus4 1Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada; 2In den Kreuzäckern 16, Tübingen, Germany; 3Immunopathological Laboratory, Department of Internal Medicine II, Otfried-Müller-Strasse, Tübingen, Germany; 4The Edinburgh Centre for Toxicology, Edinburgh, Scotland, UK Abstract: The primary objective of this “Glossary of Terms Used in Immunotoxicology” is to give clear definitions for those who contribute to studies relevant to immunotoxicology but are not themselves immunologists. This applies especially to chemists who need to under- stand the literature of immunology without recourse to a multiplicity of other glossaries or dictionaries. The glossary includes terms related to basic and clinical immunology insofar as they are necessary for a self-contained document, and particularly terms related to diagnos- ing, measuring, and understanding effects of substances on the immune system. The glossary consists of about 1200 terms as primary alphabetical entries, and Annexes of common abbre- viations, examples of chemicals with known effects on the immune system, autoantibodies in autoimmune disease, and therapeutic agents used in autoimmune disease and cancer. The authors hope that among the groups who will find this glossary helpful, in addition to chemists, are toxicologists, pharmacologists, medical practitioners, risk assessors, and regu- latory authorities. In particular, it should facilitate the worldwide use of chemistry in relation to occupational and environmental risk assessment.
    [Show full text]
  • MELISA-TEK ® RUMINANT KIT - Cat
    MMEELLIISSAA--TTEEKK®® SPECIATION KITS FOR MEAT & BONE MEALS AND ANIMAL FEEDS For the Detection of Animal Species Content Of Meat & Bone Meals and Animal Feeds by Monoclonal Enzyme Linked ImmunoSorbent Assay INSTRUCTIONS FOR USE MELISA-TEK ® RUMINANT KIT - Cat. No. SE110017 MELISA-TEK ® PORK KIT - Cat. No. SE110018 3050 Spruce Street, St. Louis, MO 63103 USA Tel: (800)521-8956 Fax: (800)325-5052 Sigma-Aldrich.com Manufactured By: ELISA Technologies, Inc. 2501 NW 66 th Court Gainesville, FL 32653 USA Tel: (352) 337-3929 Fax: (352) 337-3928 www.elisa-tek.com CONTENTS Page Number 2 TABLE OF CONTENTS 3 GENERAL DESCRIPTION 3 TEST PRINCIPLE 3 IMPORTANCE OF TROPONIN -I DETECTION 3 SAFETY INSTRUCTIONS 3 STORAGE AND STABILITY 4 MATERIA LS PROVIDED 4 MATERIALS NOT PROVIDED 5 PREPARING A PLATE PLAN 6 KIT COMPONENT PREPARATION 6 PREPARE SAMPLES TO BE TESTED 6 PREPARE THE TEST CONTROLS 7 ASSAY PROCEDURE 8 RESULTS WORKSHEET 8 PERFORMANCE CHARACTERISTICS 9 KIT SPECIFICITY 9 KIT LIMITATIONS 9 DISCLAIMER 10 SPECIES KITS LISTING 2 MELISA-TEK ® SPECIES ASSAY KIT INSTRUCTIONS General Description : The ELISA Technologies MELISA-TEK ® Species assay kit is an immunoassay for the detection of species specific troponin-I. Troponin-I is a heat stable, muscle specific protein and, as such, this kit is intended to detect muscle tissue in extracts made from cooked meat and feed products such as meat meals and meat and bone meals. Test Principle: This test is a sandwich ELISA that allows for detection of muscle tissue in extracts made from cooked meat or feed products (e.g., meat meals and meat and bone meals).
    [Show full text]
  • A Novel Lymphocyte Transformation Test (LTT-MELISAR) for Lyme Borreliosis
    Diagnostic Microbiology and Infectious Disease 57 (2007) 27–34 www.elsevier.com/locate/diagmicrobio A novel lymphocyte transformation test (LTT-MELISAR) for Lyme borreliosis Elizabeth Valentine-Thona,4, Karsten Ilsemannb, Martin Sandkampc aDepartment of Immunology, Laboratory Center Bremen, 28205 Bremen, Germany bDepartment of Serology in Laboratory Center Bremen, 28205 Bremen, Germany cLaboratory Center Bremen, 28205 Bremen, Germany Received 18 April 2006; accepted 7 June 2006 Abstract Diagnosis of active Lyme borreliosis (LB) remains a challenge in clinically ambiguous, serologically indeterminant, and polymerase chain reaction-negative patients. Lymphocyte transformation tests (LTTs) have been applied to detect specific cellular immune reactivity, but their clinical application has been severely hampered by the poorly defined Borrelia antigens and nonstandardized LTT formats used. In this study, we describe the development and clinical relevance of a novel LTT using a validated format (MELISAR) together with well-defined recombinant Borrelia-specific antigens. From an initial screening of 244 patients with suspected Borrelia infection or disease, 4 informative recombinant antigens were selected: OspC (Borrelia afzelii), p41-1 (Borrelia garinii), p41-2 (B. afzelii), and p100 (B. afzelii). Thereafter, 30 seronegative healthy controls were tested in LTT-MELISAR to determine specificity, 68 patients were tested in parallel to determine reproducibility, and 54 lymphocyte-reactive symptomatic patients were tested before and after antibiotic therapy to assess clinical relevance. Most (86.2%) of the 36.9% (90/244) LTT-MELISAR-positive patients were seropositive and showed symptoms of active LB. Specificity was 96.7% and reproducibility 92.6%. After therapy, most patients (90.7%) showed negative or markedly reduced lymphocyte reactivity correlating with clinical improvement.
    [Show full text]
  • Use of Monoclonal Antibodies in an ELISA to Detect Igm Class Antibodies Specific for Toxoplasma Gondii
    J Clin Pathol: first published as 10.1136/jcp.40.8.853 on 1 August 1987. Downloaded from J Clin Pathol 1987;40:853-857 Use of monoclonal antibodies in an ELISA to detect IgM class antibodies specific for Toxoplasma gondii A H BALFOUR, J P HARFORD, MARGARET GOODALL* From the Toxoplasma Unit, The Regional Public Health Laboratory, Leeds, and the *Department of Immunology, The Medical School, Birmingham SUMMARY Two monoclonal antibodies CH6 and Cl E3 were used in an antibody class capture assay for the detection of IgM antibodies specific for Toxoplasma gondii. CH6 was used on the solid phase to capture human IgM. After a Toxoplasma gondii antigen had been added, specifically bound material was detected using Cl E3 coupled to horseradish peroxidase. The assay was compared with an established system using polyclonal antisera at both the capture and antigen detection stages. A good correlation was found, with 97 3% (125 of 128) of sera giving the same classification in both assays. Three sera were positive only in the polyclonal system. No false positive results were found when 118 negative sera were examined. The two monoclonal antibodies provide a viable alternative to the use of polyclonal sera at the capture and antigen detection stages in the antibody class capture assay for the measurement of specific IgM against Tgondii. copyright. In many infectious diseases the presence of specific two reverse immunosorbent methods for specific IgM antibodies of the IgM class allows a diagnosis to be detection, it was not used in an enzyme linked immu- made from a single serum specimen taken early in nosorbent assay (ELISA) system.7 8 In this study an infection.
    [Show full text]